1qvu - Revision history

OCA at 10:05, 16 August 2023

OCA — Wed, 16 Aug 2023 10:05:51 GMT

←Older revision		Revision as of 10:05, 16 August 2023
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	<StructureSection load='1qvu' size='340' side='right'caption='[[1qvu]], [[Resolution\|resolution]] 2.96Å' scene=''>		<StructureSection load='1qvu' size='340' side='right'caption='[[1qvu]], [[Resolution\|resolution]] 2.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qvu]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1QVU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qvu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QVU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ET:ETHIDIUM'>ET</scene>~~, ~~<scene name='pdbligand=PRL:PROFLAVIN'>PRL</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.96Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1qvt\|1qvt]], [[1jty\|1jty]], [[1jt0\|1jt0]], [[1jus\|1jus]], [[1jt6\|1jt6]], [[1jtx\|1jtx]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ET:ETHIDIUM'>ET</scene>, <scene name='pdbligand=PRL:PROFLAVIN'>PRL</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">QACR OR SAVP031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qvu OCA], [https://pdbe.org/1qvu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qvu RCSB], [https://www.ebi.ac.uk/pdbsum/1qvu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qvu ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1qvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qvu OCA], [~~http~~://pdbe.org/1qvu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1qvu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1qvu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1qvu ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/QACR_STAAU QACR_STAAU]] Transcriptional repressor of qacA. Binds to IR1, an unusually long 28 bp operator, which is located downstream from the qacA promoter and overlaps its transcription start site. QacR is induced from its IR1 site by binding to one of many structurally dissimilar cationic lipophilic compounds, which are also substrates of QacA.	+	[https://www.uniprot.org/uniprot/QACR_STAAU QACR_STAAU] Transcriptional repressor of qacA. Binds to IR1, an unusually long 28 bp operator, which is located downstream from the qacA promoter and overlaps its transcription start site. QacR is induced from its IR1 site by binding to one of many structurally dissimilar cationic lipophilic compounds, which are also substrates of QacA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	==See Also==		==See Also==
	*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]		*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]
		+	*[[Transcriptional activator 3D structures\|Transcriptional activator 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Brennan, R G~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: ~~Miller, M C~~]]	+	[[Category: Brennan RG]]
-	[[Category: ~~Schumacher, M A~~]]	+	[[Category: Miller MC]]
-	[[Category: ~~Cooperative dna binding]]~~	+	[[Category: Schumacher MA]]
-	~~[[Category: Multidrug binding protein]]~~	+
-	~~[[Category: Qacr]]~~	+
-	~~[[Category: Repressor]]~~	+
-	~~[[Category: Transcription~~]]	+

OCA at 13:12, 16 December 2020

OCA — Wed, 16 Dec 2020 13:12:16 GMT

←Older revision		Revision as of 13:12, 16 December 2020
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	==Crystal structure of the multidrug binding transcriptional repressor QacR bound to two drugs: ethidium and proflavine==		==Crystal structure of the multidrug binding transcriptional repressor QacR bound to two drugs: ethidium and proflavine==
-	<StructureSection load='1qvu' size='340' side='right' caption='[[1qvu]], [[Resolution\|resolution]] 2.96Å' scene=''>	+	<StructureSection load='1qvu' size='340' side='right'caption='[[1qvu]], [[Resolution\|resolution]] 2.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qvu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1QVU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qvu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1QVU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ET:ETHIDIUM'>ET</scene>, <scene name='pdbligand=PRL:PROFLAVIN'>PRL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ET:ETHIDIUM'>ET</scene>, <scene name='pdbligand=PRL:PROFLAVIN'>PRL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qvt\|1qvt]], [[1jty\|1jty]], [[1jt0\|1jt0]], [[1jus\|1jus]], [[1jt6\|1jt6]], [[1jtx\|1jtx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1qvt\|1qvt]], [[1jty\|1jty]], [[1jt0\|1jt0]], [[1jus\|1jus]], [[1jt6\|1jt6]], [[1jtx\|1jtx]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QACR OR SAVP031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QACR OR SAVP031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1qvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qvu OCA], [http://pdbe.org/1qvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qvu RCSB], [http://www.ebi.ac.uk/pdbsum/1qvu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qvu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1qvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qvu OCA], [http://pdbe.org/1qvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qvu RCSB], [http://www.ebi.ac.uk/pdbsum/1qvu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qvu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1qvu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1qvu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Tetracycline repressor protein 3D structures\|Tetracycline repressor protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Brennan, R G]]		[[Category: Brennan, R G]]
	[[Category: Miller, M C]]		[[Category: Miller, M C]]

OCA at 07:52, 28 February 2018

OCA — Wed, 28 Feb 2018 07:52:33 GMT

←Older revision		Revision as of 07:52, 28 February 2018
Line 1:		Line 1:
		+
	==Crystal structure of the multidrug binding transcriptional repressor QacR bound to two drugs: ethidium and proflavine==		==Crystal structure of the multidrug binding transcriptional repressor QacR bound to two drugs: ethidium and proflavine==
	<StructureSection load='1qvu' size='340' side='right' caption='[[1qvu]], [[Resolution\|resolution]] 2.96Å' scene=''>		<StructureSection load='1qvu' size='340' side='right' caption='[[1qvu]], [[Resolution\|resolution]] 2.96Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qvt\|1qvt]], [[1jty\|1jty]], [[1jt0\|1jt0]], [[1jus\|1jus]], [[1jt6\|1jt6]], [[1jtx\|1jtx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qvt\|1qvt]], [[1jty\|1jty]], [[1jt0\|1jt0]], [[1jus\|1jus]], [[1jt6\|1jt6]], [[1jtx\|1jtx]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QACR OR SAVP031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QACR OR SAVP031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qvu OCA], [http://pdbe.org/1qvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qvu RCSB], [http://www.ebi.ac.uk/pdbsum/1qvu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qvu OCA], [http://pdbe.org/1qvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qvu RCSB], [http://www.ebi.ac.uk/pdbsum/1qvu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qvu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qv/1qvu_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qv/1qvu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:27, 8 February 2016

OCA — Mon, 08 Feb 2016 11:27:19 GMT

←Older revision		Revision as of 11:27, 8 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qvu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:05, 10 September 2015

OCA — Thu, 10 Sep 2015 11:05:46 GMT

←Older revision		Revision as of 11:05, 10 September 2015
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	<StructureSection load='1qvu' size='340' side='right' caption='[[1qvu]], [[Resolution\|resolution]] 2.96Å' scene=''>		<StructureSection load='1qvu' size='340' side='right' caption='[[1qvu]], [[Resolution\|resolution]] 2.96Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qvu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus Staphylococcus~~ aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QVU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qvu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QVU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ET:ETHIDIUM'>ET</scene>, <scene name='pdbligand=PRL:PROFLAVIN'>PRL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ET:ETHIDIUM'>ET</scene>, <scene name='pdbligand=PRL:PROFLAVIN'>PRL</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qvt\|1qvt]], [[1jty\|1jty]], [[1jt0\|1jt0]], [[1jus\|1jus]], [[1jt6\|1jt6]], [[1jtx\|1jtx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qvt\|1qvt]], [[1jty\|1jty]], [[1jt0\|1jt0]], [[1jus\|1jus]], [[1jt6\|1jt6]], [[1jtx\|1jtx]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QACR OR SAVP031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 ~~Staphylococcus~~ aureus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QACR OR SAVP031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qvu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qvu RCSB], [http://www.ebi.ac.uk/pdbsum/1qvu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qvu OCA], [http://pdbe.org/1qvu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qvu RCSB], [http://www.ebi.ac.uk/pdbsum/1qvu PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1qvu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Staphylococcus aureus]]
	[[Category: Brennan, R G]]		[[Category: Brennan, R G]]
	[[Category: Miller, M C]]		[[Category: Miller, M C]]

OCA at 22:58, 24 December 2014

OCA — Wed, 24 Dec 2014 22:58:49 GMT

←Older revision		Revision as of 22:58, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qvu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QVU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qvu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QVU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ET:ETHIDIUM'>ET</scene>, <scene name='pdbligand=PRL:PROFLAVIN'>PRL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ET:ETHIDIUM'>ET</scene>, <scene name='pdbligand=PRL:PROFLAVIN'>PRL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qvt\|1qvt]], [[1jty\|1jty]], [[1jt0\|1jt0]], [[1jus\|1jus]], [[1jt6\|1jt6]], [[1jtx\|1jtx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qvt\|1qvt]], [[1jty\|1jty]], [[1jt0\|1jt0]], [[1jus\|1jus]], [[1jt6\|1jt6]], [[1jtx\|1jtx]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QACR OR SAVP031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QACR OR SAVP031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qvu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qvu RCSB], [http://www.ebi.ac.uk/pdbsum/1qvu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qvu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qvu RCSB], [http://www.ebi.ac.uk/pdbsum/1qvu PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/QACR_STAAU QACR_STAAU]] Transcriptional repressor of qacA. Binds to IR1, an unusually long 28 bp operator, which is located downstream from the qacA promoter and overlaps its transcription start site. QacR is induced from its IR1 site by binding to one of many structurally dissimilar cationic lipophilic compounds, which are also substrates of QacA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Brennan, R G.]]	+	[[Category: Brennan, R G]]
-	[[Category: Miller, M C.]]	+	[[Category: Miller, M C]]
-	[[Category: Schumacher, M A.]]	+	[[Category: Schumacher, M A]]
	[[Category: Cooperative dna binding]]		[[Category: Cooperative dna binding]]
	[[Category: Multidrug binding protein]]		[[Category: Multidrug binding protein]]

OCA at 14:36, 29 September 2014

OCA — Mon, 29 Sep 2014 14:36:21 GMT

←Older revision		Revision as of 14:36, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1qvu.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of the multidrug binding transcriptional repressor QacR bound to two drugs: ethidium and proflavine==
		+	<StructureSection load='1qvu' size='340' side='right' caption='[[1qvu]], [[Resolution\|resolution]] 2.96Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1qvu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QVU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ET:ETHIDIUM'>ET</scene>, <scene name='pdbligand=PRL:PROFLAVIN'>PRL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qvt\|1qvt]], [[1jty\|1jty]], [[1jt0\|1jt0]], [[1jus\|1jus]], [[1jt6\|1jt6]], [[1jtx\|1jtx]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">QACR OR SAVP031 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qvu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qvu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qvu RCSB], [http://www.ebi.ac.uk/pdbsum/1qvu PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qv/1qvu_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The structural basis of simultaneous binding of two or more different drugs by any multidrug-binding protein is unknown and also how this can lead to a noncompetitive, uncompetitive or cooperative binding mechanism. Here, we describe the crystal structure of the Staphylococcus aureus multidrug-binding transcription repressor, QacR, bound simultaneously to ethidium (Et) and proflavin (Pf). The structure underscores the plasticity of the multidrug-binding pocket and reveals an alternative, Pf-induced binding mode for Et. To monitor the simultaneous binding of Pf and Et to QacR, as well as to determine the effects on the binding affinity of one drug when the other drug is prebound, a novel application of near-ultraviolet circular dichroism (UVCD) was developed. The UVCD equilibrium-binding studies revealed identical affinities of Pf for QacR in the presence or absence of Et, but significantly diminished affinity of Et for QacR when Pf is prebound, findings that are readily explicable by their structures. The principles for simultaneous binding of two different drugs discerned here are likely employed by the multidrug efflux transporters.

-	~~{{STRUCTURE_1qvu\| PDB=1qvu \| SCENE= }}~~	+	Structural mechanism of the simultaneous binding of two drugs to a multidrug-binding protein.,Schumacher MA, Miller MC, Brennan RG EMBO J. 2004 Aug 4;23(15):2923-30. Epub 2004 Jul 15. PMID:15257299<ref>PMID:15257299</ref>

-	~~===Crystal structure~~ of the ~~multidrug binding transcriptional repressor QacR bound to two drugs: ethidium and proflavine===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15257299}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1qvu]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Staphylococcus_aureus Staphylococcus aureus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015257299<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Brennan, R G.]]		[[Category: Brennan, R G.]]

OCA: Protected "1qvu" [edit=sysop:move=sysop]

OCA — Wed, 02 Jan 2013 10:10:54 GMT

Protected "1qvu" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:10, 2 January 2013

OCA at 10:10, 2 January 2013

OCA — Wed, 02 Jan 2013 10:10:52 GMT

←Older revision		Revision as of 10:10, 2 January 2013
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-	{{Seed}}
	[[Image:1qvu.png\|left\|200px]]		[[Image:1qvu.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1qvu", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1qvu\| PDB=1qvu \| SCENE= }}		{{STRUCTURE_1qvu\| PDB=1qvu \| SCENE= }}

	===Crystal structure of the multidrug binding transcriptional repressor QacR bound to two drugs: ethidium and proflavine===		===Crystal structure of the multidrug binding transcriptional repressor QacR bound to two drugs: ethidium and proflavine===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15257299}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15257299 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15257299}}		{{ABSTRACT_PUBMED_15257299}}

	==About this Structure==		==About this Structure==
-	~~1QVU~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA].	+	[[1qvu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15257299~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015257299</ref><references group="xtra"/>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Brennan, R G.]]		[[Category: Brennan, R G.]]
Line 33:		Line 21:
	[[Category: Repressor]]		[[Category: Repressor]]
	[[Category: Transcription]]		[[Category: Transcription]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:39:07 2009''

OCA at 19:39, 16 February 2009

OCA — Mon, 16 Feb 2009 19:39:08 GMT

←Older revision		Revision as of 19:39, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1QVU is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA].	+	1QVU is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVU OCA].

	==Reference==		==Reference==
-	~~Structural mechanism of the simultaneous binding of two drugs to a multidrug-binding protein., Schumacher MA, Miller MC, Brennan RG, EMBO J. 2004 Aug 4;23(15):2923-30. Epub 2004 Jul 15.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15257299 15257299]~~	+	<ref group="xtra">PMID:15257299</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Brennan, R G.]]		[[Category: Brennan, R G.]]
Line 35:		Line 34:
	[[Category: Transcription]]		[[Category: Transcription]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon ~~Jul 28 02~~:34:~~21 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 21:39:07 2009''