1qwq - Revision history

OCA at 06:07, 17 April 2024

OCA — Wed, 17 Apr 2024 06:07:54 GMT

←Older revision		Revision as of 06:07, 17 April 2024
Line 1:		Line 1:

	==Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease==		==Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease==
-	<StructureSection load='1qwq' size='340' side='right'caption='[[1qwq~~]], [[NMR_Ensembles_of_Models \| 16 NMR models~~]]' scene=''>	+	<StructureSection load='1qwq' size='340' side='right'caption='[[1qwq]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qwq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bovin Bovin~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QWQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qwq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QWQ FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1bsr\|1bsr]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRN ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [https://pdbe.org/1qwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [https://www.ebi.ac.uk/pdbsum/1qwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [https://pdbe.org/1qwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [https://www.ebi.ac.uk/pdbsum/1qwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/RNS_BOVIN RNS_BOVIN]] This enzyme hydrolyzes both single- and double-stranded RNA.	+	[https://www.uniprot.org/uniprot/RNS_BOVIN RNS_BOVIN] This enzyme hydrolyzes both single- and double-stranded RNA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qwq ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qwq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Bovine seminal ribonuclease (BS-RNase), the only dimeric protein among the pancreatic-like ribonucleases, is endowed with special structural features and with biological functions beyond enzymatic activity. In solution, the protein exists as an equilibrium mixture of two forms, with or without exchange (or swapping) of the N-terminal arms. After selective reduction and alkylation of the two intrachain disulfide bridges, the dimeric protein can be transformed into a monomeric derivative that has a ribonuclease activity higher than that of the parent dimeric protein but is devoid of the special biological functions. A detailed investigation of the structural features of this protein in solution, in comparison with those of other monomeric ribonucleases, may help unveil the structural details which induce swapping of the N-terminal arms of BS-RNase. The solution structure of the recombinant monomeric form of BS-RNase, as determined by 3D heteronuclear NMR, shows close similarity with that of bovine pancreatic ribonuclease (RNase A) in all regions characterized by regular elements of secondary structure. However, significant differences are present in the flexible regions, which could account for the different behavior of the two proteins. To characterize in detail these regions, we have measured H/D exchange rate constants, temperature coefficients and heteronuclear NOEs of backbone amides for both RNase A and monomeric BS-RNase. The results indicate a large difference in the backbone flexibility of the hinge peptide segment 16-22 of the two proteins, which could provide the molecular basis to explain the ability of BS-RNase subunits to swap their N-terminal arms.
-
-	The swapping of terminal arms in ribonucleases: comparison of the solution structure of monomeric bovine seminal and pancreatic ribonucleases.,Avitabile F, Alfano C, Spadaccini R, Crescenzi O, D'Ursi AM, D'Alessio G, Tancredi T, Picone D Biochemistry. 2003 Jul 29;42(29):8704-11. PMID:12873130<ref>PMID:12873130</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1qwq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]		*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Pancreatic ribonuclease]]~~	+	[[Category: Alfano C]]
-	~~[[Category: Alessio, G D]]~~	+	[[Category: Avitabile F]]
-	[[Category: Alfano, C]]	+	[[Category: Crescenzi O]]
-	[[Category: Avitabile, F]]	+	[[Category: D'Alessio G]]
-	[[Category: Crescenzi, O]]	+	[[Category: D'Ursi AM]]
-	[[Category: ~~Picone,~~ D]]	+	[[Category: Picone D]]
-	[[Category: ~~Spadaccini, R~~]]	+	[[Category: Spadaccini R]]
-	[[Category: ~~Tancredi, T]]~~	+	[[Category: Tancredi T]]
-	~~[[Category: Ursi, A M.~~D]]	+
-	[[Category: ~~Alpha-beta-protein~~]]	+
-	[[Category: ~~Hydrolase~~]]	+

OCA at 06:27, 10 November 2021

OCA — Wed, 10 Nov 2021 06:27:55 GMT

←Older revision		Revision as of 06:27, 10 November 2021
Line 3:		Line 3:
	<StructureSection load='1qwq' size='340' side='right'caption='[[1qwq]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='1qwq' size='340' side='right'caption='[[1qwq]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qwq]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bovin Bovin]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1QWQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qwq]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QWQ FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bsr\|1bsr]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bsr\|1bsr]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRN ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRN ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [~~http~~://pdbe.org/1qwq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1qwq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [https://pdbe.org/1qwq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [https://www.ebi.ac.uk/pdbsum/1qwq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RNS_BOVIN RNS_BOVIN]] This enzyme hydrolyzes both single- and double-stranded RNA.	+	[[https://www.uniprot.org/uniprot/RNS_BOVIN RNS_BOVIN]] This enzyme hydrolyzes both single- and double-stranded RNA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	==See Also==		==See Also==
	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]		*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	*[[Temp\|Temp]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 09:31, 26 February 2020

OCA — Wed, 26 Feb 2020 09:31:23 GMT

←Older revision		Revision as of 09:31, 26 February 2020
Line 1:		Line 1:

	==Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease==		==Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease==
-	<StructureSection load='1qwq' size='340' side='right' caption='[[1qwq]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>	+	<StructureSection load='1qwq' size='340' side='right'caption='[[1qwq]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qwq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QWQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qwq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QWQ FirstGlance]. <br>
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Ribonuclease\|Ribonuclease]]	+	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
	== References ==		== References ==
Line 39:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Bovin]]		[[Category: Bovin]]
		+	[[Category: Large Structures]]
	[[Category: Pancreatic ribonuclease]]		[[Category: Pancreatic ribonuclease]]
	[[Category: Alessio, G D]]		[[Category: Alessio, G D]]

OCA at 08:03, 28 February 2018

OCA — Wed, 28 Feb 2018 08:03:30 GMT

←Older revision		Revision as of 08:03, 28 February 2018
Line 1:		Line 1:
		+
	==Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease==		==Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease==
	<StructureSection load='1qwq' size='340' side='right' caption='[[1qwq]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='1qwq' size='340' side='right' caption='[[1qwq]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [http://pdbe.org/1qwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [http://www.ebi.ac.uk/pdbsum/1qwq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [http://pdbe.org/1qwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [http://www.ebi.ac.uk/pdbsum/1qwq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/1qwq_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/1qwq_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 17:20, 7 February 2016

OCA — Sun, 07 Feb 2016 17:20:39 GMT

←Older revision		Revision as of 17:20, 7 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qwq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:35, 10 September 2015

OCA — Thu, 10 Sep 2015 03:35:09 GMT

←Older revision		Revision as of 03:35, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1qwq' size='340' side='right' caption='[[1qwq]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>		<StructureSection load='1qwq' size='340' side='right' caption='[[1qwq]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qwq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QWQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qwq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QWQ FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bsr\|1bsr]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bsr\|1bsr]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 ~~Bos taurus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [http://www.ebi.ac.uk/pdbsum/1qwq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [http://pdbe.org/1qwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [http://www.ebi.ac.uk/pdbsum/1qwq PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1qwq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
-	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bos taurus~~]]	+	[[Category: Bovin]]
	[[Category: Pancreatic ribonuclease]]		[[Category: Pancreatic ribonuclease]]
	[[Category: Alessio, G D]]		[[Category: Alessio, G D]]

OCA at 17:00, 24 December 2014

OCA — Wed, 24 Dec 2014 17:00:21 GMT

←Older revision		Revision as of 17:00, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qwq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QWQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qwq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QWQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bsr\|1bsr]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bsr\|1bsr]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [http://www.ebi.ac.uk/pdbsum/1qwq PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [http://www.ebi.ac.uk/pdbsum/1qwq PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RNS_BOVIN RNS_BOVIN]] This enzyme hydrolyzes both single- and double-stranded RNA.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
		+	*[[Temp\|Temp]]
		+	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 39:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Pancreatic ribonuclease]]		[[Category: Pancreatic ribonuclease]]
-	[[Category: Alessio, G D.]]	+	[[Category: Alessio, G D]]
-	[[Category: Alfano, C.]]	+	[[Category: Alfano, C]]
-	[[Category: Avitabile, F.]]	+	[[Category: Avitabile, F]]
-	[[Category: Crescenzi, O.]]	+	[[Category: Crescenzi, O]]
-	[[Category: Picone, D.]]	+	[[Category: Picone, D]]
-	[[Category: Spadaccini, R.]]	+	[[Category: Spadaccini, R]]
-	[[Category: Tancredi, T.]]	+	[[Category: Tancredi, T]]
-	[[Category: Ursi, A M.D.]]	+	[[Category: Ursi, A M.D]]
	[[Category: Alpha-beta-protein]]		[[Category: Alpha-beta-protein]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 00:05, 29 September 2014

OCA — Mon, 29 Sep 2014 00:05:01 GMT

←Older revision		Revision as of 00:05, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1qwq.~~png~~\|~~left~~\|200px]]	+	==Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease==
		+	<StructureSection load='1qwq' size='340' side='right' caption='[[1qwq]], [[NMR_Ensembles_of_Models \| 16 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1qwq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QWQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bsr\|1bsr]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SRN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 Bos taurus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [http://www.ebi.ac.uk/pdbsum/1qwq PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/1qwq_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bovine seminal ribonuclease (BS-RNase), the only dimeric protein among the pancreatic-like ribonucleases, is endowed with special structural features and with biological functions beyond enzymatic activity. In solution, the protein exists as an equilibrium mixture of two forms, with or without exchange (or swapping) of the N-terminal arms. After selective reduction and alkylation of the two intrachain disulfide bridges, the dimeric protein can be transformed into a monomeric derivative that has a ribonuclease activity higher than that of the parent dimeric protein but is devoid of the special biological functions. A detailed investigation of the structural features of this protein in solution, in comparison with those of other monomeric ribonucleases, may help unveil the structural details which induce swapping of the N-terminal arms of BS-RNase. The solution structure of the recombinant monomeric form of BS-RNase, as determined by 3D heteronuclear NMR, shows close similarity with that of bovine pancreatic ribonuclease (RNase A) in all regions characterized by regular elements of secondary structure. However, significant differences are present in the flexible regions, which could account for the different behavior of the two proteins. To characterize in detail these regions, we have measured H/D exchange rate constants, temperature coefficients and heteronuclear NOEs of backbone amides for both RNase A and monomeric BS-RNase. The results indicate a large difference in the backbone flexibility of the hinge peptide segment 16-22 of the two proteins, which could provide the molecular basis to explain the ability of BS-RNase subunits to swap their N-terminal arms.

-	~~{{STRUCTURE_1qwq\| PDB=1qwq \| SCENE= }}~~	+	The swapping of terminal arms in ribonucleases: comparison of the solution structure of monomeric bovine seminal and pancreatic ribonucleases.,Avitabile F, Alfano C, Spadaccini R, Crescenzi O, D'Ursi AM, D'Alessio G, Tancredi T, Picone D Biochemistry. 2003 Jul 29;42(29):8704-11. PMID:12873130<ref>PMID:12873130</ref>

-	~~===Solution structure~~ of the ~~monomeric N67D mutant of Bovine Seminal Ribonuclease===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_12873130}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1qwq]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus]~~. ~~This structure supersedes the now removed PDB entry [http:~~/~~/oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA].~~	+

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:012873130<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Pancreatic ribonuclease]]		[[Category: Pancreatic ribonuclease]]

OCA at 07:21, 20 October 2012

OCA — Sat, 20 Oct 2012 07:21:28 GMT

←Older revision		Revision as of 07:21, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1qwq]] is a 1 chain structure ~~of [[Ribonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA].	+	[[1qwq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1o0g 1o0g]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWQ OCA].

	==See Also==		==See Also==

OCA: Protected "1qwq" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 16:03:43 GMT

Protected "1qwq" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:03, 25 July 2012