1qyd - Revision history

OCA at 08:18, 14 February 2024

OCA — Wed, 14 Feb 2024 08:18:48 GMT

←Older revision		Revision as of 08:18, 14 February 2024
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	<StructureSection load='1qyd' size='340' side='right'caption='[[1qyd]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1qyd' size='340' side='right'caption='[[1qyd]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Giant_arborvitae Giant arborvitae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QYD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thuja_plicata Thuja plicata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QYD FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>~~[[~~1qyc~~\|~~1qyc~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyd OCA], [https://pdbe.org/1qyd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qyd RCSB], [https://www.ebi.ac.uk/pdbsum/1qyd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qyd ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyd OCA], [https://pdbe.org/1qyd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qyd RCSB], [https://www.ebi.ac.uk/pdbsum/1qyd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qyd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/PILR1_THUPL PILR1_THUPL]] Reductase involved in lignan biosynthesis. Catalyzes the enantioselective sequential conversion of (-)-pinoresinol into (-)-lariciresinol and of (-)-lariciresinol into (+)-secoisolariciresinol. Can also convert with a lower efficiency (+)-pinoresinol into (+)-lariciresinol, but not (+)-lariciresinol into (-)-secoisolariciresinol. Abstracts the 4R-hydride from the NADPH cofactor during catalysis.<ref>PMID:9872995</ref>	+	[https://www.uniprot.org/uniprot/PILR1_THUPL PILR1_THUPL] Reductase involved in lignan biosynthesis. Catalyzes the enantioselective sequential conversion of (-)-pinoresinol into (-)-lariciresinol and of (-)-lariciresinol into (+)-secoisolariciresinol. Can also convert with a lower efficiency (+)-pinoresinol into (+)-lariciresinol, but not (+)-lariciresinol into (-)-secoisolariciresinol. Abstracts the 4R-hydride from the NADPH cofactor during catalysis.<ref>PMID:9872995</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qyd ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qyd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Despite the importance of plant lignans and isoflavonoids in human health protection (e.g. for both treatment and prevention of onset of various cancers) as well as in plant biology (e.g. in defense functions and in heartwood development), systematic studies on the enzymes involved in their biosynthesis have only recently begun. In this investigation, three NADPH-dependent aromatic alcohol reductases were comprehensively studied, namely pinoresinol-lariciresinol reductase (PLR), phenylcoumaran benzylic ether reductase (PCBER), and isoflavone reductase (IFR), which are involved in central steps to the various important bioactive lignans and isoflavonoids. Of particular interest was in determining how differing regio- and enantiospecificities are achieved with the different enzymes, despite each apparently going through similar enone intermediates. Initially, the three-dimensional x-ray crystal structures of both PLR_Tp1 and PCBER_Pt1 were solved and refined to 2.5 and 2.2 A resolutions, respectively. Not only do they share high gene sequence similarity, but their structures are similar, having a continuous alpha/beta NADPH-binding domain and a smaller substrate-binding domain. IFR (whose crystal structure is not yet obtained) was also compared (modeled) with PLR and PCBER and was deduced to have the same overall basic structure. The basis for the distinct enantio-specific and regio-specific reactions of PCBER, PLR, and IFR, as well as the reaction mechanism and participating residues involved (as identified by site-directed mutagenesis), are discussed.
-
-	Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases and their relationship to isoflavone reductases.,Min T, Kasahara H, Bedgar DL, Youn B, Lawrence PK, Gang DR, Halls SC, Park H, Hilsenbeck JL, Davin LB, Lewis NG, Kang C J Biol Chem. 2003 Dec 12;278(50):50714-23. Epub 2003 Sep 16. PMID:13129921<ref>PMID:13129921</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1qyd" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Giant arborvitae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bedgar~~, D L~~]]	+	[[Category: Thuja plicata]]
-	[[Category: Davin~~, L B~~]]	+	[[Category: Bedgar DL]]
-	[[Category: Gang~~, D R~~]]	+	[[Category: Davin LB]]
-	[[Category: Halls~~, S C~~]]	+	[[Category: Gang DR]]
-	[[Category: Hilsenbeck~~, J L~~]]	+	[[Category: Halls SC]]
-	[[Category: Kang, C]]	+	[[Category: Hilsenbeck JL]]
-	[[Category: Kasahara, H]]	+	[[Category: Kang C]]
-	[[Category: Lawrence~~, P K~~]]	+	[[Category: Kasahara H]]
-	[[Category: Min, T]]	+	[[Category: Lawrence PK]]
-	[[Category: Park, H]]	+	[[Category: Min T]]
-	[[Category: Youn, B]]	+	[[Category: Park H]]
-	~~[[Category: Ifr]]~~	+	[[Category: Youn B]]
-	~~[[Category: Isoflavonoid]]~~	+
-	~~[[Category: Lignan]]~~	+
-	~~[[Category: Nadph-dependent aromatic alcohol reductase]]~~	+
-	~~[[Category: Pcber]]~~	+
-	~~[[Category: Plant protein]]~~	+
-	~~[[Category: Plr~~]]	+

OCA at 10:06, 15 September 2021

OCA — Wed, 15 Sep 2021 10:06:08 GMT

←Older revision		Revision as of 10:06, 15 September 2021
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	<StructureSection load='1qyd' size='340' side='right'caption='[[1qyd]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1qyd' size='340' side='right'caption='[[1qyd]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Giant_arborvitae Giant arborvitae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1QYD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Giant_arborvitae Giant arborvitae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QYD FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qyc\|1qyc]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1qyc\|1qyc]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1qyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyd OCA], [~~http~~://pdbe.org/1qyd PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1qyd RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1qyd PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1qyd ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyd OCA], [https://pdbe.org/1qyd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qyd RCSB], [https://www.ebi.ac.uk/pdbsum/1qyd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qyd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/PILR1_THUPL PILR1_THUPL]] Reductase involved in lignan biosynthesis. Catalyzes the enantioselective sequential conversion of (-)-pinoresinol into (-)-lariciresinol and of (-)-lariciresinol into (+)-secoisolariciresinol. Can also convert with a lower efficiency (+)-pinoresinol into (+)-lariciresinol, but not (+)-lariciresinol into (-)-secoisolariciresinol. Abstracts the 4R-hydride from the NADPH cofactor during catalysis.<ref>PMID:9872995</ref>	+	[[https://www.uniprot.org/uniprot/PILR1_THUPL PILR1_THUPL]] Reductase involved in lignan biosynthesis. Catalyzes the enantioselective sequential conversion of (-)-pinoresinol into (-)-lariciresinol and of (-)-lariciresinol into (+)-secoisolariciresinol. Can also convert with a lower efficiency (+)-pinoresinol into (+)-lariciresinol, but not (+)-lariciresinol into (-)-secoisolariciresinol. Abstracts the 4R-hydride from the NADPH cofactor during catalysis.<ref>PMID:9872995</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:46, 4 December 2019

OCA — Wed, 04 Dec 2019 11:46:13 GMT

←Older revision		Revision as of 11:46, 4 December 2019
Line 1:		Line 1:

	==Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases, and their relationship to isoflavone reductases==		==Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases, and their relationship to isoflavone reductases==
-	<StructureSection load='1qyd' size='340' side='right' caption='[[1qyd]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='1qyd' size='340' side='right'caption='[[1qyd]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Giant_arborvitae Giant arborvitae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QYD FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Giant_arborvitae Giant arborvitae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QYD FirstGlance]. <br>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Giant arborvitae]]		[[Category: Giant arborvitae]]
		+	[[Category: Large Structures]]
	[[Category: Bedgar, D L]]		[[Category: Bedgar, D L]]
	[[Category: Davin, L B]]		[[Category: Davin, L B]]

OCA at 08:10, 28 February 2018

OCA — Wed, 28 Feb 2018 08:10:27 GMT

←Older revision		Revision as of 08:10, 28 February 2018
Line 1:		Line 1:
		+
	==Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases, and their relationship to isoflavone reductases==		==Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases, and their relationship to isoflavone reductases==
	<StructureSection load='1qyd' size='340' side='right' caption='[[1qyd]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1qyd' size='340' side='right' caption='[[1qyd]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Giant_arborvitae Giant arborvitae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QYD FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Giant_arborvitae Giant arborvitae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QYD FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qyc\|1qyc]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qyc\|1qyc]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyd OCA], [http://pdbe.org/1qyd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qyd RCSB], [http://www.ebi.ac.uk/pdbsum/1qyd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyd OCA], [http://pdbe.org/1qyd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qyd RCSB], [http://www.ebi.ac.uk/pdbsum/1qyd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qyd ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qy/1qyd_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qy/1qyd_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 17:37, 7 February 2016

OCA — Sun, 07 Feb 2016 17:37:00 GMT

←Older revision		Revision as of 17:37, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qyd ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:41, 10 September 2015

OCA — Thu, 10 Sep 2015 03:41:41 GMT

←Older revision		Revision as of 03:41, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1qyd' size='340' side='right' caption='[[1qyd]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1qyd' size='340' side='right' caption='[[1qyd]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thuja_plicata Thuja plicata~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QYD FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Giant_arborvitae Giant arborvitae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QYD FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qyc\|1qyc]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qyc\|1qyc]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qyd RCSB], [http://www.ebi.ac.uk/pdbsum/1qyd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyd OCA], [http://pdbe.org/1qyd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qyd RCSB], [http://www.ebi.ac.uk/pdbsum/1qyd PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q9LD14_THUPL Q9LD14_THUPL~~]] Reductase involved in lignan biosynthesis. Catalyzes the enantioselective sequential conversion of (-)-pinoresinol into (-)-lariciresinol and of (-)-lariciresinol into (+)-secoisolariciresinol. Can also convert with a lower efficiency (+)-pinoresinol into (+)-lariciresinol, but not (+)-lariciresinol into (-)-secoisolariciresinol. Abstracts the 4R-hydride from the NADPH cofactor during catalysis.<ref>PMID:9872995</ref>	+	[[http://www.uniprot.org/uniprot/PILR1_THUPL PILR1_THUPL]] Reductase involved in lignan biosynthesis. Catalyzes the enantioselective sequential conversion of (-)-pinoresinol into (-)-lariciresinol and of (-)-lariciresinol into (+)-secoisolariciresinol. Can also convert with a lower efficiency (+)-pinoresinol into (+)-lariciresinol, but not (+)-lariciresinol into (-)-secoisolariciresinol. Abstracts the 4R-hydride from the NADPH cofactor during catalysis.<ref>PMID:9872995</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1qyd" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thuja plicata~~]]	+	[[Category: Giant arborvitae]]
	[[Category: Bedgar, D L]]		[[Category: Bedgar, D L]]
	[[Category: Davin, L B]]		[[Category: Davin, L B]]

OCA at 17:07, 24 December 2014

OCA — Wed, 24 Dec 2014 17:07:46 GMT

←Older revision		Revision as of 17:07, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thuja_plicata Thuja plicata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QYD FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thuja_plicata Thuja plicata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QYD FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qyc\|1qyc]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qyc\|1qyc]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qyd RCSB], [http://www.ebi.ac.uk/pdbsum/1qyd PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qyd RCSB], [http://www.ebi.ac.uk/pdbsum/1qyd PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q9LD14_THUPL Q9LD14_THUPL]] Reductase involved in lignan biosynthesis. Catalyzes the enantioselective sequential conversion of (-)-pinoresinol into (-)-lariciresinol and of (-)-lariciresinol into (+)-secoisolariciresinol. Can also convert with a lower efficiency (+)-pinoresinol into (+)-lariciresinol, but not (+)-lariciresinol into (-)-secoisolariciresinol. Abstracts the 4R-hydride from the NADPH cofactor during catalysis.<ref>PMID:9872995</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Thuja plicata]]		[[Category: Thuja plicata]]
-	[[Category: Bedgar, D L.]]	+	[[Category: Bedgar, D L]]
-	[[Category: Davin, L B.]]	+	[[Category: Davin, L B]]
-	[[Category: Gang, D R.]]	+	[[Category: Gang, D R]]
-	[[Category: Halls, S C.]]	+	[[Category: Halls, S C]]
-	[[Category: Hilsenbeck, J L.]]	+	[[Category: Hilsenbeck, J L]]
-	[[Category: Kang, C.]]	+	[[Category: Kang, C]]
-	[[Category: Kasahara, H.]]	+	[[Category: Kasahara, H]]
-	[[Category: Lawrence, P K.]]	+	[[Category: Lawrence, P K]]
-	[[Category: Min, T.]]	+	[[Category: Min, T]]
-	[[Category: Park, H.]]	+	[[Category: Park, H]]
-	[[Category: Youn, B.]]	+	[[Category: Youn, B]]
	[[Category: Ifr]]		[[Category: Ifr]]
	[[Category: Isoflavonoid]]		[[Category: Isoflavonoid]]

OCA at 17:36, 29 September 2014

OCA — Mon, 29 Sep 2014 17:36:12 GMT

←Older revision		Revision as of 17:36, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1qyd.~~png~~\|~~left~~\|200px]]	+	==Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases, and their relationship to isoflavone reductases==
		+	<StructureSection load='1qyd' size='340' side='right' caption='[[1qyd]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1qyd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thuja_plicata Thuja plicata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QYD FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qyc\|1qyc]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qyd OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qyd RCSB], [http://www.ebi.ac.uk/pdbsum/1qyd PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qy/1qyd_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Despite the importance of plant lignans and isoflavonoids in human health protection (e.g. for both treatment and prevention of onset of various cancers) as well as in plant biology (e.g. in defense functions and in heartwood development), systematic studies on the enzymes involved in their biosynthesis have only recently begun. In this investigation, three NADPH-dependent aromatic alcohol reductases were comprehensively studied, namely pinoresinol-lariciresinol reductase (PLR), phenylcoumaran benzylic ether reductase (PCBER), and isoflavone reductase (IFR), which are involved in central steps to the various important bioactive lignans and isoflavonoids. Of particular interest was in determining how differing regio- and enantiospecificities are achieved with the different enzymes, despite each apparently going through similar enone intermediates. Initially, the three-dimensional x-ray crystal structures of both PLR_Tp1 and PCBER_Pt1 were solved and refined to 2.5 and 2.2 A resolutions, respectively. Not only do they share high gene sequence similarity, but their structures are similar, having a continuous alpha/beta NADPH-binding domain and a smaller substrate-binding domain. IFR (whose crystal structure is not yet obtained) was also compared (modeled) with PLR and PCBER and was deduced to have the same overall basic structure. The basis for the distinct enantio-specific and regio-specific reactions of PCBER, PLR, and IFR, as well as the reaction mechanism and participating residues involved (as identified by site-directed mutagenesis), are discussed.

-	~~{{STRUCTURE_1qyd\| PDB=1qyd \| SCENE= }}~~	+	Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases and their relationship to isoflavone reductases.,Min T, Kasahara H, Bedgar DL, Youn B, Lawrence PK, Gang DR, Halls SC, Park H, Hilsenbeck JL, Davin LB, Lewis NG, Kang C J Biol Chem. 2003 Dec 12;278(50):50714-23. Epub 2003 Sep 16. PMID:13129921<ref>PMID:13129921</ref>

-	~~===Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases~~, ~~and their relationship to isoflavone reductases===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_13129921}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1qyd]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Thuja_plicata Thuja plicata]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:013129921<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Thuja plicata]]		[[Category: Thuja plicata]]
	[[Category: Bedgar, D L.]]		[[Category: Bedgar, D L.]]

OCA: Protected "1qyd" [edit=sysop:move=sysop]

OCA — Wed, 02 Jan 2013 10:04:33 GMT

Protected "1qyd" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:04, 2 January 2013

OCA at 10:04, 2 January 2013

OCA — Wed, 02 Jan 2013 10:04:32 GMT

←Older revision		Revision as of 10:04, 2 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1qyd.png\|left\|200px]]		[[Image:1qyd.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1qyd", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1qyd\| PDB=1qyd \| SCENE= }}		{{STRUCTURE_1qyd\| PDB=1qyd \| SCENE= }}

	===Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases, and their relationship to isoflavone reductases===		===Crystal structures of pinoresinol-lariciresinol and phenylcoumaran benzylic ether reductases, and their relationship to isoflavone reductases===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_13129921}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 13129921 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_13129921}}		{{ABSTRACT_PUBMED_13129921}}

	==About this Structure==		==About this Structure==
-	~~1QYD~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Thuja_plicata Thuja plicata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA].	+	[[1qyd]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thuja_plicata Thuja plicata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QYD OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~13129921~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:013129921</ref><references group="xtra"/>
	[[Category: Thuja plicata]]		[[Category: Thuja plicata]]
	[[Category: Bedgar, D L.]]		[[Category: Bedgar, D L.]]
Line 41:		Line 29:
	[[Category: Nadph-dependent aromatic alcohol reductase]]		[[Category: Nadph-dependent aromatic alcohol reductase]]
	[[Category: Pcber]]		[[Category: Pcber]]
		+	[[Category: Plant protein]]
	[[Category: Plr]]		[[Category: Plr]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 15:28:39 2009''