1qzg - Revision history

OCA at 08:19, 14 February 2024

OCA — Wed, 14 Feb 2024 08:19:11 GMT

←Older revision		Revision as of 08:19, 14 February 2024
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	<StructureSection load='1qzg' size='340' side='right'caption='[[1qzg]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1qzg' size='340' side='right'caption='[[1qzg]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qzg]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cbs_356 Cbs 356~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1QZG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qzg]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=TMP:THYMIDINE~~-~~5-PHOSPHATE'>TMP</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1qzh\|1qzh]]~~</~~div~~>~~</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TMP:THYMIDINE-5-PHOSPHATE'>TMP</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzg OCA], [https://pdbe.org/1qzg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qzg RCSB], [https://www.ebi.ac.uk/pdbsum/1qzg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzg ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1qzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzg OCA], [~~http~~://pdbe.org/1qzg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1qzg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1qzg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzg ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/POT1_SCHPO POT1_SCHPO]] Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length.<ref>PMID:11349150</ref> <ref>PMID:12463756</ref> <ref>PMID:18535244</ref>	+	[https://www.uniprot.org/uniprot/POT1_SCHPO POT1_SCHPO] Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length.<ref>PMID:11349150</ref> <ref>PMID:12463756</ref> <ref>PMID:18535244</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qzg ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qzg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Telomeres, specialized protein-DNA complexes that cap the ends of linear chromosomes, are essential for protecting chromosomes from degradation and end-to-end fusions. The Pot1 (protection of telomeres 1) protein is a widely distributed eukaryotic end-capping protein, having been identified in fission yeast, microsporidia, plants and animals. Schizosaccharomyces pombe Pot1p is essential for telomere maintenance, and human POT1 has been implicated in telomerase regulation. Pot1 binds telomeric single-stranded DNA (ssDNA) with exceptionally high sequence specificity, the molecular basis of which has been unknown. Here we describe the 1.9-A-resolution crystal structure of the amino-terminal DNA-binding domain of S. pombe Pot1p complexed with ssDNA. The protein adopts an oligonucleotide/oligosaccharide-binding (OB) fold with two loops that protrude to form a clamp for ssDNA binding. The structure explains the sequence specificity of binding: in the context of the Pot1 protein, DNA self-recognition involving base-stacking and unusual G-T base pairs compacts the DNA. Any sequence change disrupts the ability of the DNA to form this structure, preventing it from contacting the array of protein hydrogen-bonding groups. The structure also explains how Pot1p avoids binding the vast excess of RNA in the nucleus.
-
-	DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA.,Lei M, Podell ER, Baumann P, Cech TR Nature. 2003 Nov 13;426(6963):198-203. PMID:14614509<ref>PMID:14614509</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1qzg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cbs 356]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Baumann, P~~]]	+	[[Category: Schizosaccharomyces pombe]]
-	[[Category: ~~Cech, T R~~]]	+	[[Category: Baumann P]]
-	[[Category: ~~Lei, M~~]]	+	[[Category: Cech TR]]
-	[[Category: ~~Podell, E R~~]]	+	[[Category: Lei M]]
-	[[Category: ~~Dna binding protein-dna complex]]~~	+	[[Category: Podell ER]]
-	~~[[Category: Protrein-dna complex]]~~	+
-	~~[[Category: Single-stranded telomeric dna~~]]	+

OCA at 13:14, 16 December 2020

OCA — Wed, 16 Dec 2020 13:14:45 GMT

←Older revision		Revision as of 13:14, 16 December 2020
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	==Crystal structure of Pot1 (protection of telomere)- ssDNA complex==		==Crystal structure of Pot1 (protection of telomere)- ssDNA complex==
-	<StructureSection load='1qzg' size='340' side='right' caption='[[1qzg]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1qzg' size='340' side='right'caption='[[1qzg]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qzg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1QZG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qzg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1QZG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMP:THYMIDINE-5-PHOSPHATE'>TMP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TMP:THYMIDINE-5-PHOSPHATE'>TMP</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qzh\|1qzh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1qzh\|1qzh]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1qzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzg OCA], [http://pdbe.org/1qzg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qzg RCSB], [http://www.ebi.ac.uk/pdbsum/1qzg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1qzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzg OCA], [http://pdbe.org/1qzg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qzg RCSB], [http://www.ebi.ac.uk/pdbsum/1qzg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</StructureSection>		</StructureSection>
	[[Category: Cbs 356]]		[[Category: Cbs 356]]
		+	[[Category: Large Structures]]
	[[Category: Baumann, P]]		[[Category: Baumann, P]]
	[[Category: Cech, T R]]		[[Category: Cech, T R]]

OCA at 07:45, 28 February 2018

OCA — Wed, 28 Feb 2018 07:45:11 GMT

←Older revision		Revision as of 07:45, 28 February 2018
Line 1:		Line 1:
		+
	==Crystal structure of Pot1 (protection of telomere)- ssDNA complex==		==Crystal structure of Pot1 (protection of telomere)- ssDNA complex==
	<StructureSection load='1qzg' size='340' side='right' caption='[[1qzg]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1qzg' size='340' side='right' caption='[[1qzg]], [[Resolution\|resolution]] 1.90Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qzh\|1qzh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qzh\|1qzh]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzg OCA], [http://pdbe.org/1qzg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qzg RCSB], [http://www.ebi.ac.uk/pdbsum/1qzg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzg OCA], [http://pdbe.org/1qzg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qzg RCSB], [http://www.ebi.ac.uk/pdbsum/1qzg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/1qzg_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/1qzg_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:00, 9 February 2016

OCA — Tue, 09 Feb 2016 11:00:42 GMT

←Older revision		Revision as of 11:00, 9 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qzg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:39, 10 September 2015

OCA — Thu, 10 Sep 2015 21:39:15 GMT

←Older revision		Revision as of 21:39, 10 September 2015
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	<StructureSection load='1qzg' size='340' side='right' caption='[[1qzg]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1qzg' size='340' side='right' caption='[[1qzg]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qzg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Schizosaccharomyces_pombe Schizosaccharomyces pombe~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qzg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMP:THYMIDINE-5-PHOSPHATE'>TMP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMP:THYMIDINE-5-PHOSPHATE'>TMP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qzh\|1qzh]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qzh\|1qzh]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 ~~Schizosaccharomyces pombe~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 CBS 356])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qzg RCSB], [http://www.ebi.ac.uk/pdbsum/1qzg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzg OCA], [http://pdbe.org/1qzg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qzg RCSB], [http://www.ebi.ac.uk/pdbsum/1qzg PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1qzg" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Schizosaccharomyces pombe~~]]	+	[[Category: Cbs 356]]
	[[Category: Baumann, P]]		[[Category: Baumann, P]]
	[[Category: Cech, T R]]		[[Category: Cech, T R]]

OCA at 06:26, 25 December 2014

OCA — Thu, 25 Dec 2014 06:26:40 GMT

←Older revision		Revision as of 06:26, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qzg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qzg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMP:THYMIDINE-5-PHOSPHATE'>TMP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMP:THYMIDINE-5-PHOSPHATE'>TMP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qzh\|1qzh]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qzh\|1qzh]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qzg RCSB], [http://www.ebi.ac.uk/pdbsum/1qzg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qzg RCSB], [http://www.ebi.ac.uk/pdbsum/1qzg PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/POT1_SCHPO POT1_SCHPO]] Single-stranded telomeric DNA-binding protein that is required to protect the 3'-end telomeric overhang. It binds the consensus sequence 5'-GGTTAC-3'. Regulates telomerase and telomere length.<ref>PMID:11349150</ref> <ref>PMID:12463756</ref> <ref>PMID:18535244</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Schizosaccharomyces pombe]]		[[Category: Schizosaccharomyces pombe]]
-	[[Category: Baumann, P.]]	+	[[Category: Baumann, P]]
-	[[Category: Cech, T R.]]	+	[[Category: Cech, T R]]
-	[[Category: Lei, M.]]	+	[[Category: Lei, M]]
-	[[Category: Podell, E R.]]	+	[[Category: Podell, E R]]
	[[Category: Dna binding protein-dna complex]]		[[Category: Dna binding protein-dna complex]]
	[[Category: Protrein-dna complex]]		[[Category: Protrein-dna complex]]
	[[Category: Single-stranded telomeric dna]]		[[Category: Single-stranded telomeric dna]]

OCA at 17:22, 29 September 2014

OCA — Mon, 29 Sep 2014 17:22:31 GMT

←Older revision		Revision as of 17:22, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1qzg.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of Pot1 (protection of telomere)- ssDNA complex==
		+	<StructureSection load='1qzg' size='340' side='right' caption='[[1qzg]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1qzg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TMP:THYMIDINE-5-PHOSPHATE'>TMP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qzh\|1qzh]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">POT1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qzg RCSB], [http://www.ebi.ac.uk/pdbsum/1qzg PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/1qzg_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Telomeres, specialized protein-DNA complexes that cap the ends of linear chromosomes, are essential for protecting chromosomes from degradation and end-to-end fusions. The Pot1 (protection of telomeres 1) protein is a widely distributed eukaryotic end-capping protein, having been identified in fission yeast, microsporidia, plants and animals. Schizosaccharomyces pombe Pot1p is essential for telomere maintenance, and human POT1 has been implicated in telomerase regulation. Pot1 binds telomeric single-stranded DNA (ssDNA) with exceptionally high sequence specificity, the molecular basis of which has been unknown. Here we describe the 1.9-A-resolution crystal structure of the amino-terminal DNA-binding domain of S. pombe Pot1p complexed with ssDNA. The protein adopts an oligonucleotide/oligosaccharide-binding (OB) fold with two loops that protrude to form a clamp for ssDNA binding. The structure explains the sequence specificity of binding: in the context of the Pot1 protein, DNA self-recognition involving base-stacking and unusual G-T base pairs compacts the DNA. Any sequence change disrupts the ability of the DNA to form this structure, preventing it from contacting the array of protein hydrogen-bonding groups. The structure also explains how Pot1p avoids binding the vast excess of RNA in the nucleus.

-	~~{{STRUCTURE_1qzg\| PDB=1qzg \| SCENE= }}~~	+	DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA.,Lei M, Podell ER, Baumann P, Cech TR Nature. 2003 Nov 13;426(6963):198-203. PMID:14614509<ref>PMID:14614509</ref>

-	~~===Crystal structure~~ of ~~Pot1 (protection of telomere)- ssDNA complex===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14614509}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1qzg]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:014614509<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Schizosaccharomyces pombe]]		[[Category: Schizosaccharomyces pombe]]
	[[Category: Baumann, P.]]		[[Category: Baumann, P.]]

OCA: Protected "1qzg" [edit=sysop:move=sysop]

OCA — Fri, 04 Jan 2013 10:14:22 GMT

Protected "1qzg" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:14, 4 January 2013

OCA at 10:14, 4 January 2013

OCA — Fri, 04 Jan 2013 10:14:19 GMT

←Older revision		Revision as of 10:14, 4 January 2013
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-	{{Seed}}
	[[Image:1qzg.png\|left\|200px]]		[[Image:1qzg.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1qzg", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1qzg\| PDB=1qzg \| SCENE= }}		{{STRUCTURE_1qzg\| PDB=1qzg \| SCENE= }}

	===Crystal structure of Pot1 (protection of telomere)- ssDNA complex===		===Crystal structure of Pot1 (protection of telomere)- ssDNA complex===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_14614509}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 14614509 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_14614509}}		{{ABSTRACT_PUBMED_14614509}}

	==About this Structure==		==About this Structure==
-	~~1QZG~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA].	+	[[1qzg]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~14614509~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014614509</ref><references group="xtra"/>
	[[Category: Schizosaccharomyces pombe]]		[[Category: Schizosaccharomyces pombe]]
	[[Category: Baumann, P.]]		[[Category: Baumann, P.]]
Line 29:		Line 17:
	[[Category: Lei, M.]]		[[Category: Lei, M.]]
	[[Category: Podell, E R.]]		[[Category: Podell, E R.]]
		+	[[Category: Dna binding protein-dna complex]]
	[[Category: Protrein-dna complex]]		[[Category: Protrein-dna complex]]
	[[Category: Single-stranded telomeric dna]]		[[Category: Single-stranded telomeric dna]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 09:20:24 2009''

OCA at 07:20, 17 February 2009

OCA — Tue, 17 Feb 2009 07:20:26 GMT

←Older revision		Revision as of 07:20, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1QZG is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA].	+	1QZG is a 4 chains structure of sequences from [http://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZG OCA].

	==Reference==		==Reference==
-	~~DNA self-recognition in the structure of Pot1 bound to telomeric single-stranded DNA., Lei M, Podell ER, Baumann P, Cech TR, Nature. 2003 Nov 13;426(6963):198-203.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/14614509 14614509]~~	+	<ref group="xtra">PMID:14614509</ref><references group="xtra"/>
	[[Category: Schizosaccharomyces pombe]]		[[Category: Schizosaccharomyces pombe]]
-	[[Category: Single protein]]
	[[Category: Baumann, P.]]		[[Category: Baumann, P.]]
	[[Category: Cech, T R.]]		[[Category: Cech, T R.]]
Line 33:		Line 32:
	[[Category: Single-stranded telomeric dna]]		[[Category: Single-stranded telomeric dna]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Mon Jul 28 12~~:53:~~57 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 09:20:24 2009''