1qzp - Revision history

OCA at 09:02, 22 May 2024

OCA — Wed, 22 May 2024 09:02:41 GMT

←Older revision		Revision as of 09:02, 22 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qzp ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qzp ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dematin (band 4.9) is found in the junctional complex of the spectrin cytoskeleton that supports the erythrocyte cell membrane. Dematin is a member of the larger class of cytoskeleton-associated proteins that contain a modular "headpiece" domain at their extreme C termini. The dematin headpiece domain provides the second F-actin-binding site required for in vitro F-actin bundling. The dematin headpiece is found in two forms in the cell, one of 68 residues (DHP) and one containing a 22-amino acid insert near its N terminus (DHP+22). In addition, dematin contains the only headpiece domain that is phosphorylated, in vivo. The 22-amino acid insert in DHP+22 appeared unstructured in NMR spectra; therefore, we have determined the three-dimensional structure of DHP by multidimensional NMR methods. Although the overall three-dimensional structure of DHP is similar to that of the villin headpiece, there are two novel characteristics revealed by this structure. First, unlike villin headpiece that contains a single buried salt bridge, DHP contains a buried charged cluster comprising residues Glu(39), Arg(66), Lys(70), and the C-terminal carboxylate of Phe(76). Second, (15)N relaxation experiments indicate that the longer "variable loop" region near the N terminus of DHP (residues 20-29) is dynamic, undergoing significantly greater motions that the rest of the structure. Furthermore, NMR chemical shift changes indicate that the conformation of the dynamic variable loop is altered by phosphorylation of serine 74, which is far in the sequence from the variable loop region. Our results suggest that phosphorylation of the dematin headpiece acts as a conformational switch within this headpiece domain.
		+
		+	The NMR structure of dematin headpiece reveals a dynamic loop that is conformationally altered upon phosphorylation at a distal site.,Frank BS, Vardar D, Chishti AH, McKnight CJ J Biol Chem. 2004 Feb 27;279(9):7909-16. Epub 2003 Dec 2. PMID:14660664<ref>PMID:14660664</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1qzp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:08, 17 April 2024

OCA — Wed, 17 Apr 2024 06:08:22 GMT

←Older revision		Revision as of 06:08, 17 April 2024
Line 1:		Line 1:

	==NMR structure of the human dematin headpiece domain==		==NMR structure of the human dematin headpiece domain==
-	<StructureSection load='1qzp' size='340' side='right'caption='[[1qzp~~]], [[NMR_Ensembles_of_Models \| 13 NMR models~~]]' scene=''>	+	<StructureSection load='1qzp' size='340' side='right'caption='[[1qzp]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZP FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1qqv\|1qqv]], [[1vii\|1vii]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [https://pdbe.org/1qzp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [https://www.ebi.ac.uk/pdbsum/1qzp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzp ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [https://pdbe.org/1qzp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [https://www.ebi.ac.uk/pdbsum/1qzp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/DEMA_HUMAN DEMA_HUMAN]] Actin-bundling protein. May function in mitogen-activated protein kinase pathway.<ref>PMID:11856323</ref>	+	[https://www.uniprot.org/uniprot/DEMA_HUMAN DEMA_HUMAN] Actin-bundling protein. May function in mitogen-activated protein kinase pathway.<ref>PMID:11856323</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qzp ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qzp ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Dematin (band 4.9) is found in the junctional complex of the spectrin cytoskeleton that supports the erythrocyte cell membrane. Dematin is a member of the larger class of cytoskeleton-associated proteins that contain a modular "headpiece" domain at their extreme C termini. The dematin headpiece domain provides the second F-actin-binding site required for in vitro F-actin bundling. The dematin headpiece is found in two forms in the cell, one of 68 residues (DHP) and one containing a 22-amino acid insert near its N terminus (DHP+22). In addition, dematin contains the only headpiece domain that is phosphorylated, in vivo. The 22-amino acid insert in DHP+22 appeared unstructured in NMR spectra; therefore, we have determined the three-dimensional structure of DHP by multidimensional NMR methods. Although the overall three-dimensional structure of DHP is similar to that of the villin headpiece, there are two novel characteristics revealed by this structure. First, unlike villin headpiece that contains a single buried salt bridge, DHP contains a buried charged cluster comprising residues Glu(39), Arg(66), Lys(70), and the C-terminal carboxylate of Phe(76). Second, (15)N relaxation experiments indicate that the longer "variable loop" region near the N terminus of DHP (residues 20-29) is dynamic, undergoing significantly greater motions that the rest of the structure. Furthermore, NMR chemical shift changes indicate that the conformation of the dynamic variable loop is altered by phosphorylation of serine 74, which is far in the sequence from the variable loop region. Our results suggest that phosphorylation of the dematin headpiece acts as a conformational switch within this headpiece domain.
-
-	The NMR structure of dematin headpiece reveals a dynamic loop that is conformationally altered upon phosphorylation at a distal site.,Frank BS, Vardar D, Chishti AH, McKnight CJ J Biol Chem. 2004 Feb 27;279(9):7909-16. Epub 2003 Dec 2. PMID:14660664<ref>PMID:14660664</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1qzp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chishti~~, A H~~]]	+	[[Category: Chishti AH]]
-	[[Category: Frank~~, B S~~]]	+	[[Category: Frank BS]]
-	[[Category: McKnight~~, C J~~]]	+	[[Category: McKnight CJ]]
-	[[Category: Vardar, D]]	+	[[Category: Vardar D]]
-	~~[[Category: Actin binding domain]]~~	+
-	~~[[Category: Dematin headpiece]]~~	+
-	~~[[Category: Protein binding]]~~	+
-	~~[[Category: Villin headpiece~~]]	+

OCA at 10:06, 15 September 2021

OCA — Wed, 15 Sep 2021 10:06:36 GMT

←Older revision		Revision as of 10:06, 15 September 2021
Line 3:		Line 3:
	<StructureSection load='1qzp' size='340' side='right'caption='[[1qzp]], [[NMR_Ensembles_of_Models \| 13 NMR models]]' scene=''>		<StructureSection load='1qzp' size='340' side='right'caption='[[1qzp]], [[NMR_Ensembles_of_Models \| 13 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1QZP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1QZP FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qqv\|1qqv]], [[1vii\|1vii]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1qqv\|1qqv]], [[1vii\|1vii]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [~~http~~://pdbe.org/1qzp PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1qzp PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzp ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [https://pdbe.org/1qzp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [https://www.ebi.ac.uk/pdbsum/1qzp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DEMA_HUMAN DEMA_HUMAN]] Actin-bundling protein. May function in mitogen-activated protein kinase pathway.<ref>PMID:11856323</ref>	+	[[https://www.uniprot.org/uniprot/DEMA_HUMAN DEMA_HUMAN]] Actin-bundling protein. May function in mitogen-activated protein kinase pathway.<ref>PMID:11856323</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:29, 4 December 2019

OCA — Wed, 04 Dec 2019 11:29:28 GMT

←Older revision		Revision as of 11:29, 4 December 2019
Line 1:		Line 1:

	==NMR structure of the human dematin headpiece domain==		==NMR structure of the human dematin headpiece domain==
-	<StructureSection load='1qzp' size='340' side='right' caption='[[1qzp]], [[NMR_Ensembles_of_Models \| 13 NMR models]]' scene=''>	+	<StructureSection load='1qzp' size='340' side='right'caption='[[1qzp]], [[NMR_Ensembles_of_Models \| 13 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZP FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZP FirstGlance]. <br>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Chishti, A H]]		[[Category: Chishti, A H]]
	[[Category: Frank, B S]]		[[Category: Frank, B S]]

OCA at 07:53, 28 February 2018

OCA — Wed, 28 Feb 2018 07:53:52 GMT

←Older revision		Revision as of 07:53, 28 February 2018
Line 1:		Line 1:
		+
	==NMR structure of the human dematin headpiece domain==		==NMR structure of the human dematin headpiece domain==
	<StructureSection load='1qzp' size='340' side='right' caption='[[1qzp]], [[NMR_Ensembles_of_Models \| 13 NMR models]]' scene=''>		<StructureSection load='1qzp' size='340' side='right' caption='[[1qzp]], [[NMR_Ensembles_of_Models \| 13 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZP FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZP FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qqv\|1qqv]], [[1vii\|1vii]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qqv\|1qqv]], [[1vii\|1vii]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [http://pdbe.org/1qzp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [http://www.ebi.ac.uk/pdbsum/1qzp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [http://pdbe.org/1qzp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [http://www.ebi.ac.uk/pdbsum/1qzp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qzp ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/1qzp_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/1qzp_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 02:43, 8 February 2016

OCA — Mon, 08 Feb 2016 02:43:50 GMT

←Older revision		Revision as of 02:43, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1qzp ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:07, 10 September 2015

OCA — Thu, 10 Sep 2015 07:07:00 GMT

←Older revision		Revision as of 07:07, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1qzp' size='340' side='right' caption='[[1qzp]], [[NMR_Ensembles_of_Models \| 13 NMR models]]' scene=''>		<StructureSection load='1qzp' size='340' side='right' caption='[[1qzp]], [[NMR_Ensembles_of_Models \| 13 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZP FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZP FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qqv\|1qqv]], [[1vii\|1vii]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qqv\|1qqv]], [[1vii\|1vii]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [http://www.ebi.ac.uk/pdbsum/1qzp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [http://pdbe.org/1qzp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [http://www.ebi.ac.uk/pdbsum/1qzp PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1qzp" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Chishti, A H]]		[[Category: Chishti, A H]]
	[[Category: Frank, B S]]		[[Category: Frank, B S]]

OCA at 19:59, 24 December 2014

OCA — Wed, 24 Dec 2014 19:59:52 GMT

←Older revision		Revision as of 19:59, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZP FirstGlance]. <br>		<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZP FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qqv\|1qqv]], [[1vii\|1vii]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qqv\|1qqv]], [[1vii\|1vii]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [http://www.ebi.ac.uk/pdbsum/1qzp PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [http://www.ebi.ac.uk/pdbsum/1qzp PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DEMA_HUMAN DEMA_HUMAN]] Actin-bundling protein. May function in mitogen-activated protein kinase pathway.<ref>PMID:11856323</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Chishti, A H.]]	+	[[Category: Chishti, A H]]
-	[[Category: Frank, B S.]]	+	[[Category: Frank, B S]]
-	[[Category: McKnight, C J.]]	+	[[Category: McKnight, C J]]
-	[[Category: Vardar, D.]]	+	[[Category: Vardar, D]]
	[[Category: Actin binding domain]]		[[Category: Actin binding domain]]
	[[Category: Dematin headpiece]]		[[Category: Dematin headpiece]]
	[[Category: Protein binding]]		[[Category: Protein binding]]
	[[Category: Villin headpiece]]		[[Category: Villin headpiece]]

OCA at 16:02, 29 September 2014

OCA — Mon, 29 Sep 2014 16:02:18 GMT

←Older revision		Revision as of 16:02, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1qzp.~~png~~\|~~left~~\|200px]]	+	==NMR structure of the human dematin headpiece domain==
		+	<StructureSection load='1qzp' size='340' side='right' caption='[[1qzp]], [[NMR_Ensembles_of_Models \| 13 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1qzp]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1QZP FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1qqv\|1qqv]], [[1vii\|1vii]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qzp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qzp OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1qzp RCSB], [http://www.ebi.ac.uk/pdbsum/1qzp PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qz/1qzp_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dematin (band 4.9) is found in the junctional complex of the spectrin cytoskeleton that supports the erythrocyte cell membrane. Dematin is a member of the larger class of cytoskeleton-associated proteins that contain a modular "headpiece" domain at their extreme C termini. The dematin headpiece domain provides the second F-actin-binding site required for in vitro F-actin bundling. The dematin headpiece is found in two forms in the cell, one of 68 residues (DHP) and one containing a 22-amino acid insert near its N terminus (DHP+22). In addition, dematin contains the only headpiece domain that is phosphorylated, in vivo. The 22-amino acid insert in DHP+22 appeared unstructured in NMR spectra; therefore, we have determined the three-dimensional structure of DHP by multidimensional NMR methods. Although the overall three-dimensional structure of DHP is similar to that of the villin headpiece, there are two novel characteristics revealed by this structure. First, unlike villin headpiece that contains a single buried salt bridge, DHP contains a buried charged cluster comprising residues Glu(39), Arg(66), Lys(70), and the C-terminal carboxylate of Phe(76). Second, (15)N relaxation experiments indicate that the longer "variable loop" region near the N terminus of DHP (residues 20-29) is dynamic, undergoing significantly greater motions that the rest of the structure. Furthermore, NMR chemical shift changes indicate that the conformation of the dynamic variable loop is altered by phosphorylation of serine 74, which is far in the sequence from the variable loop region. Our results suggest that phosphorylation of the dematin headpiece acts as a conformational switch within this headpiece domain.

-	~~{{STRUCTURE_1qzp\| PDB=1qzp \| SCENE= }}~~	+	The NMR structure of dematin headpiece reveals a dynamic loop that is conformationally altered upon phosphorylation at a distal site.,Frank BS, Vardar D, Chishti AH, McKnight CJ J Biol Chem. 2004 Feb 27;279(9):7909-16. Epub 2003 Dec 2. PMID:14660664<ref>PMID:14660664</ref>

-	~~===NMR structure~~ of the ~~human dematin headpiece domain===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14660664}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1qzp]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1QZP OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:014660664<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Chishti, A H.]]		[[Category: Chishti, A H.]]

OCA: Protected "1qzp" [edit=sysop:move=sysop]

OCA — Fri, 04 Jan 2013 10:26:09 GMT

Protected "1qzp" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:26, 4 January 2013