1r9c - Revision history

OCA at 06:02, 23 August 2023

OCA — Wed, 23 Aug 2023 06:02:16 GMT

←Older revision		Revision as of 06:02, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1r9c' size='340' side='right'caption='[[1r9c]], [[Resolution\|resolution]] 1.83Å' scene=''>		<StructureSection load='1r9c' size='340' side='right'caption='[[1r9c]], [[Resolution\|resolution]] 1.83Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1r9c]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_700743 Atcc 700743~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1R9C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1r9c]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1R9C FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.83Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>fosfomycin resistance protein ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~381 ATCC 700743])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1r9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9c OCA], [https://pdbe.org/1r9c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1r9c RCSB], [https://www.ebi.ac.uk/pdbsum/1r9c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1r9c ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1r9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9c OCA], [~~http~~://pdbe.org/1r9c PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1r9c RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1r9c PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1r9c ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/FOSX_RHILO FOSX_RHILO]] Catalyzes the hydration of fosfomycin.	+	[https://www.uniprot.org/uniprot/FOSX_RHILO FOSX_RHILO] Catalyzes the hydration of fosfomycin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 700743]]
-	[[Category: Dimethylallyltranstransferase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Armstrong, R N~~]]	+	[[Category: Mesorhizobium loti]]
-	[[Category: ~~Fillgrove, K L~~]]	+	[[Category: Armstrong RN]]
-	[[Category: ~~Newcomer, M E~~]]	+	[[Category: Fillgrove KL]]
-	[[Category: ~~Pakhomova, S~~]]	+	[[Category: Newcomer ME]]
-	[[Category: ~~Antibiotic resistance]]~~	+	[[Category: Pakhomova S]]
-	~~[[Category: Fosfomycin resistance protein]]~~	+
-	~~[[Category: Mn binding]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 13:21, 16 December 2020

OCA — Wed, 16 Dec 2020 13:21:49 GMT

←Older revision		Revision as of 13:21, 16 December 2020
Line 1:		Line 1:

	==Crystal Structure of Fosfomycin Resistance Protein FosX from Mesorhizobium Loti==		==Crystal Structure of Fosfomycin Resistance Protein FosX from Mesorhizobium Loti==
-	<StructureSection load='1r9c' size='340' side='right' caption='[[1r9c]], [[Resolution\|resolution]] 1.83Å' scene=''>	+	<StructureSection load='1r9c' size='340' side='right'caption='[[1r9c]], [[Resolution\|resolution]] 1.83Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1r9c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_700743 Atcc 700743]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1R9C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1r9c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_700743 Atcc 700743]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1R9C FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fosfomycin resistance protein ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381 ATCC 700743])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fosfomycin resistance protein ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381 ATCC 700743])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1r9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9c OCA], [http://pdbe.org/1r9c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r9c RCSB], [http://www.ebi.ac.uk/pdbsum/1r9c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1r9c ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1r9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9c OCA], [http://pdbe.org/1r9c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r9c RCSB], [http://www.ebi.ac.uk/pdbsum/1r9c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1r9c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 36:		Line 36:
	[[Category: Atcc 700743]]		[[Category: Atcc 700743]]
	[[Category: Dimethylallyltranstransferase]]		[[Category: Dimethylallyltranstransferase]]
		+	[[Category: Large Structures]]
	[[Category: Armstrong, R N]]		[[Category: Armstrong, R N]]
	[[Category: Fillgrove, K L]]		[[Category: Fillgrove, K L]]

OCA at 07:51, 28 February 2018

OCA — Wed, 28 Feb 2018 07:51:29 GMT

←Older revision		Revision as of 07:51, 28 February 2018
Line 1:		Line 1:
		+
	==Crystal Structure of Fosfomycin Resistance Protein FosX from Mesorhizobium Loti==		==Crystal Structure of Fosfomycin Resistance Protein FosX from Mesorhizobium Loti==
	<StructureSection load='1r9c' size='340' side='right' caption='[[1r9c]], [[Resolution\|resolution]] 1.83Å' scene=''>		<StructureSection load='1r9c' size='340' side='right' caption='[[1r9c]], [[Resolution\|resolution]] 1.83Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1r9c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Atcc_33669~~ Atcc ~~33669~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R9C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1r9c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_700743 Atcc 700743]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R9C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fosfomycin resistance protein ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381 ATCC ~~33669~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fosfomycin resistance protein ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381 ATCC 700743])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9c OCA], [http://pdbe.org/1r9c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r9c RCSB], [http://www.ebi.ac.uk/pdbsum/1r9c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9c OCA], [http://pdbe.org/1r9c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r9c RCSB], [http://www.ebi.ac.uk/pdbsum/1r9c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1r9c ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r9/1r9c_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r9/1r9c_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc ~~33669~~]]	+	[[Category: Atcc 700743]]
	[[Category: Dimethylallyltranstransferase]]		[[Category: Dimethylallyltranstransferase]]
	[[Category: Armstrong, R N]]		[[Category: Armstrong, R N]]

OCA at 14:50, 8 February 2016

OCA — Mon, 08 Feb 2016 14:50:31 GMT

←Older revision		Revision as of 14:50, 8 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1r9c ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 12:39, 10 September 2015

OCA — Thu, 10 Sep 2015 12:39:44 GMT

←Older revision		Revision as of 12:39, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1r9c' size='340' side='right' caption='[[1r9c]], [[Resolution\|resolution]] 1.83Å' scene=''>		<StructureSection load='1r9c' size='340' side='right' caption='[[1r9c]], [[Resolution\|resolution]] 1.83Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1r9c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mesorhizobium_loti Mesorhizobium loti~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R9C FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1r9c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33669 Atcc 33669]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R9C FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fosfomycin resistance protein ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381 ~~Mesorhizobium loti~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fosfomycin resistance protein ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381 ATCC 33669])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1r9c RCSB], [http://www.ebi.ac.uk/pdbsum/1r9c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9c OCA], [http://pdbe.org/1r9c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1r9c RCSB], [http://www.ebi.ac.uk/pdbsum/1r9c PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1r9c" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 33669]]
	[[Category: Dimethylallyltranstransferase]]		[[Category: Dimethylallyltranstransferase]]
-	[[Category: Mesorhizobium loti]]
	[[Category: Armstrong, R N]]		[[Category: Armstrong, R N]]
	[[Category: Fillgrove, K L]]		[[Category: Fillgrove, K L]]

OCA at 00:08, 25 December 2014

OCA — Thu, 25 Dec 2014 00:08:38 GMT

←Older revision		Revision as of 00:08, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1r9c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R9C FirstGlance]. <br>		<table><tr><td colspan='2'>[[1r9c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R9C FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fosfomycin resistance protein ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381 Mesorhizobium loti])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fosfomycin resistance protein ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381 Mesorhizobium loti])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1r9c RCSB], [http://www.ebi.ac.uk/pdbsum/1r9c PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1r9c RCSB], [http://www.ebi.ac.uk/pdbsum/1r9c PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FOSX_RHILO FOSX_RHILO]] Catalyzes the hydration of fosfomycin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	[[Category: Dimethylallyltranstransferase]]		[[Category: Dimethylallyltranstransferase]]
	[[Category: Mesorhizobium loti]]		[[Category: Mesorhizobium loti]]
-	[[Category: Armstrong, R N.]]	+	[[Category: Armstrong, R N]]
-	[[Category: Fillgrove, K L.]]	+	[[Category: Fillgrove, K L]]
-	[[Category: Newcomer, M E.]]	+	[[Category: Newcomer, M E]]
-	[[Category: Pakhomova, S.]]	+	[[Category: Pakhomova, S]]
	[[Category: Antibiotic resistance]]		[[Category: Antibiotic resistance]]
	[[Category: Fosfomycin resistance protein]]		[[Category: Fosfomycin resistance protein]]
	[[Category: Mn binding]]		[[Category: Mn binding]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 15:32, 29 September 2014

OCA — Mon, 29 Sep 2014 15:32:03 GMT

←Older revision		Revision as of 15:32, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1r9c.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Fosfomycin Resistance Protein FosX from Mesorhizobium Loti==
		+	<StructureSection load='1r9c' size='340' side='right' caption='[[1r9c]], [[Resolution\|resolution]] 1.83Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1r9c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1R9C FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">fosfomycin resistance protein ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=381 Mesorhizobium loti])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dimethylallyltranstransferase Dimethylallyltranstransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.1 2.5.1.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1r9c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1r9c OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1r9c RCSB], [http://www.ebi.ac.uk/pdbsum/1r9c PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/r9/1r9c_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microbial resistance to the antibiotic fosfomycin [(1R,2S)-epoxypropylphosphonic acid, 1] is known to be mediated by thiol transferase enzymes FosA and FosB, which catalyze the addition of glutathione and l-cysteine to C1 of the oxirane, respectively. A probe of the microbial genome database reveals a related group of enzymes (FosX). The genes mlr3345 from Mesorhizobium loti and lmo1702 from Listeria monocytogenes were cloned and the proteins expressed. This heretofore unrecognized group of enzymes is shown to catalyze the Mn(II)-dependent addition of water to C1 of the oxirane. The ability of each enzyme to confer resistance in Escherichia coli is correlated with their catalytic efficiency, such that the M. loti protein confers low resistance while the Listeria enzyme confers very robust resistance. The crystal structure of the FosX from M. loti was solved at a resolution of 1.83 A. The structure reveals an active-site carboxylate (E44) located about 5 A from the expected position of the substrate that appears to be poised to participate in catalysis. Single turnover experiments in H218O and kinetic analysis of the E44G mutant of the FosX enzymes indicate that the carboxylate of E44 acts as a general base in the direct addition of water to 1. The FosX from M. loti also catalyzes the addition of glutathione to the antibiotic. The catalytic promiscuity and low efficiency of the M. loti protein suggest that it may be an intermediate in the evolution of clinically relevant fosfomycin resistance proteins such as the FosX from Listeria monocytogenese.

-	~~{{STRUCTURE_1r9c\| PDB=1r9c \| SCENE= }}~~	+	Mechanistic diversity of fosfomycin resistance in pathogenic microorganisms.,Fillgrove KL, Pakhomova S, Newcomer ME, Armstrong RN J Am Chem Soc. 2003 Dec 24;125(51):15730-1. PMID:14677948<ref>PMID:14677948</ref>

-	~~===Crystal Structure~~ of ~~Fosfomycin Resistance Protein FosX from Mesorhizobium Loti===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14677948}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1r9c]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mesorhizobium_loti Mesorhizobium loti]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:014677948<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Dimethylallyltranstransferase]]		[[Category: Dimethylallyltranstransferase]]
	[[Category: Mesorhizobium loti]]		[[Category: Mesorhizobium loti]]

OCA: Protected "1r9c" [edit=sysop:move=sysop]

OCA — Fri, 04 Jan 2013 10:22:27 GMT

Protected "1r9c" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:22, 4 January 2013

OCA at 10:22, 4 January 2013

OCA — Fri, 04 Jan 2013 10:22:23 GMT

←Older revision		Revision as of 10:22, 4 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1r9c.png\|left\|200px]]		[[Image:1r9c.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1r9c", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1r9c\| PDB=1r9c \| SCENE= }}		{{STRUCTURE_1r9c\| PDB=1r9c \| SCENE= }}

	===Crystal Structure of Fosfomycin Resistance Protein FosX from Mesorhizobium Loti===		===Crystal Structure of Fosfomycin Resistance Protein FosX from Mesorhizobium Loti===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_14677948}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 14677948 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_14677948}}		{{ABSTRACT_PUBMED_14677948}}

	==About this Structure==		==About this Structure==
-	~~1R9C~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA].	+	[[1r9c]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~14677948~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014677948</ref><references group="xtra"/>
	[[Category: Dimethylallyltranstransferase]]		[[Category: Dimethylallyltranstransferase]]
	[[Category: Mesorhizobium loti]]		[[Category: Mesorhizobium loti]]
Line 33:		Line 21:
	[[Category: Fosfomycin resistance protein]]		[[Category: Fosfomycin resistance protein]]
	[[Category: Mn binding]]		[[Category: Mn binding]]
-		+	[[Category: Transferase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 23:47:30 2009''~~	+

OCA at 21:47, 16 February 2009

OCA — Mon, 16 Feb 2009 21:47:32 GMT

←Older revision		Revision as of 21:47, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1R9C is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA].	+	1R9C is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Mesorhizobium_loti Mesorhizobium loti]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9C OCA].

	==Reference==		==Reference==
-	~~Mechanistic diversity of fosfomycin resistance in pathogenic microorganisms., Fillgrove KL, Pakhomova S, Newcomer ME, Armstrong RN, J Am Chem Soc. 2003 Dec 24;125(51):15730-1.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/14677948 14677948]~~	+	<ref group="xtra">PMID:14677948</ref><references group="xtra"/>
	[[Category: Dimethylallyltranstransferase]]		[[Category: Dimethylallyltranstransferase]]
	[[Category: Mesorhizobium loti]]		[[Category: Mesorhizobium loti]]
-	[[Category: Single protein]]
	[[Category: Armstrong, R N.]]		[[Category: Armstrong, R N.]]
	[[Category: Fillgrove, K L.]]		[[Category: Fillgrove, K L.]]
Line 35:		Line 34:
	[[Category: Mn binding]]		[[Category: Mn binding]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon ~~Jul 28 04~~:06:~~58 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 23:47:30 2009''