1rcy - Revision history

OCA at 08:22, 14 February 2024

OCA — Wed, 14 Feb 2024 08:22:17 GMT

←Older revision		Revision as of 08:22, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1rcy' size='340' side='right'caption='[[1rcy]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1rcy' size='340' side='right'caption='[[1rcy]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"ferrobacillus_ferrooxidans"_leathen_and_braley_1954 "ferrobacillus~~ ferrooxidans~~" leathen and braley 1954~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RCY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RCY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcy OCA], [https://pdbe.org/1rcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rcy RCSB], [https://www.ebi.ac.uk/pdbsum/1rcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rcy ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcy OCA], [https://pdbe.org/1rcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rcy RCSB], [https://www.ebi.ac.uk/pdbsum/1rcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rcy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~RUS2_THIFE RUS2_THIFE]~~] Electron carrier from cytochrome c552 to the A-type oxidase.	+	[https://www.uniprot.org/uniprot/RUS2_ACIFI RUS2_ACIFI] Electron carrier from cytochrome c552 to the A-type oxidase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rcy ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rcy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The X-ray crystal structure of the oxidized form of the extremely stable and highly oxidizing cupredoxin rusticyanin from Thiobacillus ferrooxidans has been determined by the method of multiwavelength anomalous diffraction (MAD) and refined to 1.9 A resolution. Like other cupredoxins, rusticyanin is a copper-containing metalloprotein, which is composed of a core beta-sandwich fold. In rusticyanin the beta-sandwich is composed of a six- and a seven-stranded beta-sheet. Also like other cupredoxins, the copper ion is coordinated by a cluster of four conserved residues (His85, Cys138, His143, Met148) arranged in a distorted tetrahedron. Rusticyanin has a redox potential of 680 mV, roughly twice that of any other cupredoxin, and it is optimally active at pH values < or = 2. By comparison with other cupredoxins, the three-dimensional structure of rusticyanin reveals several possible sources of the chemical differences, including more ordered secondary structure and more intersheet connectivity than other cupredoxins. The acid stability and redox potential of rusticyanin may also be enhanced over other cupredoxins by a more extensive internal hydrogen bonding network and by more extensive hydrophobic interactions surrounding the copper binding site. Finally, reduction in the number of charged residues surrounding the active site may also make a major contribution to acid stability. We propose that the resulting rigid copper binding site, which is constrained by the surrounding hydrophobic environment, structurally and electronically favours Cu(I). We propose that the two extreme chemical properties of rusticyanin are interrelated; the same unique structural features that enhance acid stability also lead to elevated redox potential.
-
-	Multiple wavelength anomalous diffraction (MAD) crystal structure of rusticyanin: a highly oxidizing cupredoxin with extreme acid stability.,Walter RL, Ealick SE, Friedman AM, Blake RC 2nd, Proctor P, Shoham M J Mol Biol. 1996 Nov 15;263(5):730-51. PMID:8947572<ref>PMID:8947572</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1rcy" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Ferrobacillus~~ ferrooxidans ~~leathen and braley 1954~~]]	+	[[Category: Acidithiobacillus ferrooxidans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Ealick, S E~~]]	+	[[Category: Blake II RC]]
-	[[Category: ~~Friedman, A M~~]]	+	[[Category: Ealick SE]]
-	[[Category: ~~II, R C.Blake~~]]	+	[[Category: Friedman AM]]
-	[[Category: Proctor, P]]	+	[[Category: Proctor P]]
-	[[Category: Shoham, M]]	+	[[Category: Shoham M]]
-	[[Category: Walter~~, R L]]~~	+	[[Category: Walter RL]]
-	~~[[Category: Copper containing protein]]~~	+
-	~~[[Category: Metalloprotein]]~~	+
-	~~[[Category: Oxidation potential]]~~	+
-	~~[[Category: Ph stability]]~~	+
-	~~[[Category: Redox protein~~]]	+

OCA at 10:11, 15 September 2021

OCA — Wed, 15 Sep 2021 10:11:36 GMT

←Older revision		Revision as of 10:11, 15 September 2021
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	<StructureSection load='1rcy' size='340' side='right'caption='[[1rcy]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1rcy' size='340' side='right'caption='[[1rcy]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"ferrobacillus_ferrooxidans"_leathen_and_braley_1954 "ferrobacillus ferrooxidans" leathen and braley 1954]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1RCY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"ferrobacillus_ferrooxidans"_leathen_and_braley_1954 "ferrobacillus ferrooxidans" leathen and braley 1954]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RCY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1rcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcy OCA], [~~http~~://pdbe.org/1rcy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1rcy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1rcy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1rcy ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcy OCA], [https://pdbe.org/1rcy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rcy RCSB], [https://www.ebi.ac.uk/pdbsum/1rcy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rcy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/RUS2_THIFE RUS2_THIFE]] Electron carrier from cytochrome c552 to the A-type oxidase.	+	[[https://www.uniprot.org/uniprot/RUS2_THIFE RUS2_THIFE]] Electron carrier from cytochrome c552 to the A-type oxidase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:13, 4 December 2019

OCA — Wed, 04 Dec 2019 11:13:53 GMT

←Older revision		Revision as of 11:13, 4 December 2019
Line 1:		Line 1:

	==RUSTICYANIN (RC) FROM THIOBACILLUS FERROOXIDANS==		==RUSTICYANIN (RC) FROM THIOBACILLUS FERROOXIDANS==
-	<StructureSection load='1rcy' size='340' side='right' caption='[[1rcy]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1rcy' size='340' side='right'caption='[[1rcy]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"ferrobacillus_ferrooxidans"_leathen_and_braley_1954 "ferrobacillus ferrooxidans" leathen and braley 1954]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RCY FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"ferrobacillus_ferrooxidans"_leathen_and_braley_1954 "ferrobacillus ferrooxidans" leathen and braley 1954]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RCY FirstGlance]. <br>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Ferrobacillus ferrooxidans leathen and braley 1954]]		[[Category: Ferrobacillus ferrooxidans leathen and braley 1954]]
		+	[[Category: Large Structures]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]
	[[Category: Friedman, A M]]		[[Category: Friedman, A M]]

OCA at 07:39, 28 February 2018

OCA — Wed, 28 Feb 2018 07:39:27 GMT

←Older revision		Revision as of 07:39, 28 February 2018
Line 1:		Line 1:
		+
	==RUSTICYANIN (RC) FROM THIOBACILLUS FERROOXIDANS==		==RUSTICYANIN (RC) FROM THIOBACILLUS FERROOXIDANS==
	<StructureSection load='1rcy' size='340' side='right' caption='[[1rcy]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1rcy' size='340' side='right' caption='[[1rcy]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"ferrobacillus_ferrooxidans"_leathen_and_braley_1954 "ferrobacillus ferrooxidans" leathen and braley 1954]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RCY FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"ferrobacillus_ferrooxidans"_leathen_and_braley_1954 "ferrobacillus ferrooxidans" leathen and braley 1954]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RCY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcy OCA], [http://pdbe.org/1rcy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rcy RCSB], [http://www.ebi.ac.uk/pdbsum/1rcy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcy OCA], [http://pdbe.org/1rcy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rcy RCSB], [http://www.ebi.ac.uk/pdbsum/1rcy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rcy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/1rcy_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/1rcy_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 23:22, 9 February 2016

OCA — Tue, 09 Feb 2016 23:22:38 GMT

←Older revision		Revision as of 23:22, 9 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rcy ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:03, 11 September 2015

OCA — Fri, 11 Sep 2015 03:03:59 GMT

←Older revision		Revision as of 03:03, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1rcy' size='340' side='right' caption='[[1rcy]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1rcy' size='340' side='right' caption='[[1rcy]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Acidithiobacillus_ferrooxidans Acidithiobacillus~~ ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RCY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"ferrobacillus_ferrooxidans"_leathen_and_braley_1954 "ferrobacillus ferrooxidans" leathen and braley 1954]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RCY FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rcy RCSB], [http://www.ebi.ac.uk/pdbsum/1rcy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcy OCA], [http://pdbe.org/1rcy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rcy RCSB], [http://www.ebi.ac.uk/pdbsum/1rcy PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1rcy" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Acidithiobacillus~~ ferrooxidans]]	+	[[Category: Ferrobacillus ferrooxidans leathen and braley 1954]]
	[[Category: Ealick, S E]]		[[Category: Ealick, S E]]
	[[Category: Friedman, A M]]		[[Category: Friedman, A M]]

OCA at 10:12, 25 December 2014

OCA — Thu, 25 Dec 2014 10:12:40 GMT

←Older revision		Revision as of 10:12, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RCY FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RCY FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rcy RCSB], [http://www.ebi.ac.uk/pdbsum/1rcy PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rcy RCSB], [http://www.ebi.ac.uk/pdbsum/1rcy PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RUS2_THIFE RUS2_THIFE]] Electron carrier from cytochrome c552 to the A-type oxidase.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Acidithiobacillus ferrooxidans]]		[[Category: Acidithiobacillus ferrooxidans]]
-	[[Category: Ealick, S E.]]	+	[[Category: Ealick, S E]]
-	[[Category: Friedman, A M.]]	+	[[Category: Friedman, A M]]
-	[[Category: II, R C.Blake.]]	+	[[Category: II, R C.Blake]]
-	[[Category: Proctor, P.]]	+	[[Category: Proctor, P]]
-	[[Category: Shoham, M.]]	+	[[Category: Shoham, M]]
-	[[Category: Walter, R L.]]	+	[[Category: Walter, R L]]
	[[Category: Copper containing protein]]		[[Category: Copper containing protein]]
	[[Category: Metalloprotein]]		[[Category: Metalloprotein]]

OCA at 16:50, 29 September 2014

OCA — Mon, 29 Sep 2014 16:50:08 GMT

←Older revision		Revision as of 16:50, 29 September 2014
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-	[[~~Image~~:1rcy.~~png\|left~~\|200px]]	+	==RUSTICYANIN (RC) FROM THIOBACILLUS FERROOXIDANS==
		+	<StructureSection load='1rcy' size='340' side='right' caption='[[1rcy]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1rcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RCY FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rcy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rcy OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rcy RCSB], [http://www.ebi.ac.uk/pdbsum/1rcy PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/1rcy_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The X-ray crystal structure of the oxidized form of the extremely stable and highly oxidizing cupredoxin rusticyanin from Thiobacillus ferrooxidans has been determined by the method of multiwavelength anomalous diffraction (MAD) and refined to 1.9 A resolution. Like other cupredoxins, rusticyanin is a copper-containing metalloprotein, which is composed of a core beta-sandwich fold. In rusticyanin the beta-sandwich is composed of a six- and a seven-stranded beta-sheet. Also like other cupredoxins, the copper ion is coordinated by a cluster of four conserved residues (His85, Cys138, His143, Met148) arranged in a distorted tetrahedron. Rusticyanin has a redox potential of 680 mV, roughly twice that of any other cupredoxin, and it is optimally active at pH values < or = 2. By comparison with other cupredoxins, the three-dimensional structure of rusticyanin reveals several possible sources of the chemical differences, including more ordered secondary structure and more intersheet connectivity than other cupredoxins. The acid stability and redox potential of rusticyanin may also be enhanced over other cupredoxins by a more extensive internal hydrogen bonding network and by more extensive hydrophobic interactions surrounding the copper binding site. Finally, reduction in the number of charged residues surrounding the active site may also make a major contribution to acid stability. We propose that the resulting rigid copper binding site, which is constrained by the surrounding hydrophobic environment, structurally and electronically favours Cu(I). We propose that the two extreme chemical properties of rusticyanin are interrelated; the same unique structural features that enhance acid stability also lead to elevated redox potential.

-	~~{{STRUCTURE_1rcy\| PDB=1rcy \| SCENE= }}~~	+	Multiple wavelength anomalous diffraction (MAD) crystal structure of rusticyanin: a highly oxidizing cupredoxin with extreme acid stability.,Walter RL, Ealick SE, Friedman AM, Blake RC 2nd, Proctor P, Shoham M J Mol Biol. 1996 Nov 15;263(5):730-51. PMID:8947572<ref>PMID:8947572</ref>

-	~~===RUSTICYANIN (RC) FROM THIOBACILLUS FERROOXIDANS===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_8947572}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1rcy]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:008947572<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Acidithiobacillus ferrooxidans]]		[[Category: Acidithiobacillus ferrooxidans]]
	[[Category: Ealick, S E.]]		[[Category: Ealick, S E.]]

OCA: Protected "1rcy" [edit=sysop:move=sysop]

OCA — Fri, 04 Jan 2013 10:06:38 GMT

Protected "1rcy" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:06, 4 January 2013

OCA at 10:06, 4 January 2013

OCA — Fri, 04 Jan 2013 10:06:35 GMT

←Older revision		Revision as of 10:06, 4 January 2013
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-	{{Seed}}
	[[Image:1rcy.png\|left\|200px]]		[[Image:1rcy.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1rcy", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1rcy\| PDB=1rcy \| SCENE= }}		{{STRUCTURE_1rcy\| PDB=1rcy \| SCENE= }}

	===RUSTICYANIN (RC) FROM THIOBACILLUS FERROOXIDANS===		===RUSTICYANIN (RC) FROM THIOBACILLUS FERROOXIDANS===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_8947572}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 8947572 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_8947572}}		{{ABSTRACT_PUBMED_8947572}}

	==About this Structure==		==About this Structure==
-	~~1RCY~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA].	+	[[1rcy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Acidithiobacillus_ferrooxidans Acidithiobacillus ferrooxidans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RCY OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~8947572~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008947572</ref><references group="xtra"/>
	[[Category: Acidithiobacillus ferrooxidans]]		[[Category: Acidithiobacillus ferrooxidans]]
	[[Category: Ealick, S E.]]		[[Category: Ealick, S E.]]
Line 36:		Line 24:
	[[Category: Ph stability]]		[[Category: Ph stability]]
	[[Category: Redox protein]]		[[Category: Redox protein]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 13:33:46 2009''