1re6 - Revision history

OCA at 09:04, 22 May 2024

OCA — Wed, 22 May 2024 09:04:28 GMT

←Older revision		Revision as of 09:04, 22 May 2024
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1re6 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1re6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The dynein and myosin V motor complexes are multi-protein structures that function to transport molecules and organelles within the cell. DLC (dynein light-chain) proteins, found as components of both dynein and myosin V motor complexes, connect the complexes to their cargoes. One of the roles of these motor complexes is to selectively sequester the pro-apoptotic 'BH3-only' (Bcl-2 homology 3-only) proteins, Bim (Bcl-2-interacting mediator of cell death) and Bmf (Bcl-2-modifying factor), and so regulate their cell death-inducing function. In vivo DLC2 is found exclusively as a component of the myosin V motor complex and Bmf binds DLC2 selectively. On the other hand, Bim interacts with DLC1 (LC8), an integral component of the dynein motor complex. The two DLCs share 93% sequence identity yet show unambiguous in vivo specificity for their respective BH3-only ligands. To investigate this specificity the three-dimensional solution structure of DLC2 was elucidated using NMR spectroscopy. In vitro structural and mutagenesis studies show that Bmf and Bim have identical binding characteristics to recombinant DLC2 or DLC1. Thus the selectivity shown by Bmf and Bim for binding DLC1 or DLC2, respectively, does not reside in their DLC-binding domains. Remarkably, mutational analysis of DLC1 and DLC2 indicates that a single surface residue (residue 41) determines the specific localization of DLCs with their respective motor complexes. These results suggest a molecular mechanism for the specific compartmentalization of DLCs and their pro-apoptotic cargoes and implicate other protein(s) in defining the specificity between the cargoes and the DLC proteins.
		+
		+	Localization of dynein light chains 1 and 2 and their pro-apoptotic ligands.,Day CL, Puthalakath H, Skea G, Strasser A, Barsukov I, Lian LY, Huang DC, Hinds MG Biochem J. 2004 Feb 1;377(Pt 3):597-605. PMID:14561217<ref>PMID:14561217</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 1re6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Dynein 3D structures\|Dynein 3D structures]]		*[[Dynein 3D structures\|Dynein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 08:22, 1 May 2024

OCA — Wed, 01 May 2024 08:22:28 GMT

←Older revision		Revision as of 08:22, 1 May 2024
Line 1:		Line 1:

	==Localisation of Dynein Light Chains 1 and 2 and their Pro-apoptotic Ligands==		==Localisation of Dynein Light Chains 1 and 2 and their Pro-apoptotic Ligands==
-	<StructureSection load='1re6' size='340' side='right'caption='[[1re6~~]], [[NMR_Ensembles_of_Models \| 20 NMR models~~]]' scene=''>	+	<StructureSection load='1re6' size='340' side='right'caption='[[1re6]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>DLC2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~10090 LK3 transgenic mice])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [https://pdbe.org/1re6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [https://www.ebi.ac.uk/pdbsum/1re6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1re6 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [https://pdbe.org/1re6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [https://www.ebi.ac.uk/pdbsum/1re6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1re6 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DYL2_MOUSE DYL2_MOUSE] Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1re6 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1re6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The dynein and myosin V motor complexes are multi-protein structures that function to transport molecules and organelles within the cell. DLC (dynein light-chain) proteins, found as components of both dynein and myosin V motor complexes, connect the complexes to their cargoes. One of the roles of these motor complexes is to selectively sequester the pro-apoptotic 'BH3-only' (Bcl-2 homology 3-only) proteins, Bim (Bcl-2-interacting mediator of cell death) and Bmf (Bcl-2-modifying factor), and so regulate their cell death-inducing function. In vivo DLC2 is found exclusively as a component of the myosin V motor complex and Bmf binds DLC2 selectively. On the other hand, Bim interacts with DLC1 (LC8), an integral component of the dynein motor complex. The two DLCs share 93% sequence identity yet show unambiguous in vivo specificity for their respective BH3-only ligands. To investigate this specificity the three-dimensional solution structure of DLC2 was elucidated using NMR spectroscopy. In vitro structural and mutagenesis studies show that Bmf and Bim have identical binding characteristics to recombinant DLC2 or DLC1. Thus the selectivity shown by Bmf and Bim for binding DLC1 or DLC2, respectively, does not reside in their DLC-binding domains. Remarkably, mutational analysis of DLC1 and DLC2 indicates that a single surface residue (residue 41) determines the specific localization of DLCs with their respective motor complexes. These results suggest a molecular mechanism for the specific compartmentalization of DLCs and their pro-apoptotic cargoes and implicate other protein(s) in defining the specificity between the cargoes and the DLC proteins.
-
-	Localization of dynein light chains 1 and 2 and their pro-apoptotic ligands.,Day CL, Puthalakath H, Skea G, Strasser A, Barsukov I, Lian LY, Huang DC, Hinds MG Biochem J. 2004 Feb 1;377(Pt 3):597-605. PMID:14561217<ref>PMID:14561217</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1re6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Dynein 3D structures\|Dynein 3D structures]]		*[[Dynein 3D structures\|Dynein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Barsukov, I]]	+	[[Category: Barsukov I]]
-	[[Category: Day~~, C L~~]]	+	[[Category: Day CL]]
-	[[Category: Hinds~~, M G~~]]	+	[[Category: Hinds MG]]
-	[[Category: Huang~~, D C~~]]	+	[[Category: Huang DC]]
-	[[Category: Lian~~, L Y~~]]	+	[[Category: Lian LY]]
-	[[Category: Puthalakath, H]]	+	[[Category: Puthalakath H]]
-	[[Category: Skea, G]]	+	[[Category: Skea G]]
-	[[Category: Strasser, A]]	+	[[Category: Strasser A]]
-	~~[[Category: Apoptosis]]~~	+
-	~~[[Category: Contractile protein]]~~	+
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Dynein light chain~~]]	+

OCA at 06:30, 2 March 2022

OCA — Wed, 02 Mar 2022 06:30:24 GMT

←Older revision		Revision as of 06:30, 2 March 2022
Line 3:		Line 3:
	<StructureSection load='1re6' size='340' side='right'caption='[[1re6]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1re6' size='340' side='right'caption='[[1re6]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DLC2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DLC2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [~~http~~://pdbe.org/1re6 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1re6 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1re6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [https://pdbe.org/1re6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [https://www.ebi.ac.uk/pdbsum/1re6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1re6 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 13:24, 16 December 2020

OCA — Wed, 16 Dec 2020 13:24:27 GMT

←Older revision		Revision as of 13:24, 16 December 2020
Line 1:		Line 1:

	==Localisation of Dynein Light Chains 1 and 2 and their Pro-apoptotic Ligands==		==Localisation of Dynein Light Chains 1 and 2 and their Pro-apoptotic Ligands==
-	<StructureSection load='1re6' size='340' side='right' caption='[[1re6]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>	+	<StructureSection load='1re6' size='340' side='right'caption='[[1re6]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DLC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DLC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [http://pdbe.org/1re6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [http://www.ebi.ac.uk/pdbsum/1re6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1re6 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [http://pdbe.org/1re6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [http://www.ebi.ac.uk/pdbsum/1re6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1re6 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 1re6" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1re6" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Dynein 3D structures\|Dynein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Barsukov, I]]		[[Category: Barsukov, I]]

OCA at 07:32, 28 February 2018

OCA — Wed, 28 Feb 2018 07:32:57 GMT

←Older revision		Revision as of 07:32, 28 February 2018
Line 1:		Line 1:
		+
	==Localisation of Dynein Light Chains 1 and 2 and their Pro-apoptotic Ligands==		==Localisation of Dynein Light Chains 1 and 2 and their Pro-apoptotic Ligands==
	<StructureSection load='1re6' size='340' side='right' caption='[[1re6]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1re6' size='340' side='right' caption='[[1re6]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DLC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DLC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [http://pdbe.org/1re6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [http://www.ebi.ac.uk/pdbsum/1re6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [http://pdbe.org/1re6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [http://www.ebi.ac.uk/pdbsum/1re6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1re6 ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/re/1re6_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/re/1re6_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 25:		Line 26:
	</div>		</div>
	<div class="pdbe-citations 1re6" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1re6" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Dynein\|Dynein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 17:55, 7 February 2016

OCA — Sun, 07 Feb 2016 17:55:13 GMT

←Older revision		Revision as of 17:55, 7 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1re6 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:49, 10 September 2015

OCA — Thu, 10 Sep 2015 03:49:07 GMT

←Older revision		Revision as of 03:49, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1re6' size='340' side='right' caption='[[1re6]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>		<StructureSection load='1re6' size='340' side='right' caption='[[1re6]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DLC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DLC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [http://www.ebi.ac.uk/pdbsum/1re6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [http://pdbe.org/1re6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [http://www.ebi.ac.uk/pdbsum/1re6 PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1re6" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 31:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Barsukov, I]]		[[Category: Barsukov, I]]
	[[Category: Day, C L]]		[[Category: Day, C L]]

OCA at 06:34, 6 January 2015

OCA — Tue, 06 Jan 2015 06:34:38 GMT

←Older revision		Revision as of 06:34, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DLC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DLC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [http://www.ebi.ac.uk/pdbsum/1re6 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [http://www.ebi.ac.uk/pdbsum/1re6 PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Barsukov, I.]]	+	[[Category: Barsukov, I]]
-	[[Category: Day, C L.]]	+	[[Category: Day, C L]]
-	[[Category: Hinds, M G.]]	+	[[Category: Hinds, M G]]
-	[[Category: Huang, D C.]]	+	[[Category: Huang, D C]]
-	[[Category: Lian, L Y.]]	+	[[Category: Lian, L Y]]
-	[[Category: Puthalakath, H.]]	+	[[Category: Puthalakath, H]]
-	[[Category: Skea, G.]]	+	[[Category: Skea, G]]
-	[[Category: Strasser, A.]]	+	[[Category: Strasser, A]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Contractile protein]]		[[Category: Contractile protein]]
	[[Category: Dimer]]		[[Category: Dimer]]
	[[Category: Dynein light chain]]		[[Category: Dynein light chain]]

OCA at 19:14, 28 September 2014

OCA — Sun, 28 Sep 2014 19:14:05 GMT

←Older revision		Revision as of 19:14, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1re6.~~png\|left~~\|200px]]	+	==Localisation of Dynein Light Chains 1 and 2 and their Pro-apoptotic Ligands==
		+	<StructureSection load='1re6' size='340' side='right' caption='[[1re6]], [[NMR_Ensembles_of_Models \| 20 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1re6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RE6 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">DLC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1re6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1re6 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1re6 RCSB], [http://www.ebi.ac.uk/pdbsum/1re6 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/re/1re6_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The dynein and myosin V motor complexes are multi-protein structures that function to transport molecules and organelles within the cell. DLC (dynein light-chain) proteins, found as components of both dynein and myosin V motor complexes, connect the complexes to their cargoes. One of the roles of these motor complexes is to selectively sequester the pro-apoptotic 'BH3-only' (Bcl-2 homology 3-only) proteins, Bim (Bcl-2-interacting mediator of cell death) and Bmf (Bcl-2-modifying factor), and so regulate their cell death-inducing function. In vivo DLC2 is found exclusively as a component of the myosin V motor complex and Bmf binds DLC2 selectively. On the other hand, Bim interacts with DLC1 (LC8), an integral component of the dynein motor complex. The two DLCs share 93% sequence identity yet show unambiguous in vivo specificity for their respective BH3-only ligands. To investigate this specificity the three-dimensional solution structure of DLC2 was elucidated using NMR spectroscopy. In vitro structural and mutagenesis studies show that Bmf and Bim have identical binding characteristics to recombinant DLC2 or DLC1. Thus the selectivity shown by Bmf and Bim for binding DLC1 or DLC2, respectively, does not reside in their DLC-binding domains. Remarkably, mutational analysis of DLC1 and DLC2 indicates that a single surface residue (residue 41) determines the specific localization of DLCs with their respective motor complexes. These results suggest a molecular mechanism for the specific compartmentalization of DLCs and their pro-apoptotic cargoes and implicate other protein(s) in defining the specificity between the cargoes and the DLC proteins.

-	~~{{STRUCTURE_1re6\| PDB=1re6 \| SCENE= }}~~	+	Localization of dynein light chains 1 and 2 and their pro-apoptotic ligands.,Day CL, Puthalakath H, Skea G, Strasser A, Barsukov I, Lian LY, Huang DC, Hinds MG Biochem J. 2004 Feb 1;377(Pt 3):597-605. PMID:14561217<ref>PMID:14561217</ref>

-	~~===Localisation~~ of ~~Dynein Light Chains 1 and 2 and their Pro-apoptotic Ligands===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14561217}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1re6]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full experimental information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA].~~	+

	==See Also==		==See Also==
	*[[Dynein\|Dynein]]		*[[Dynein\|Dynein]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:014561217<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Barsukov, I.]]		[[Category: Barsukov, I.]]

OCA at 07:26, 20 October 2012

OCA — Sat, 20 Oct 2012 07:26:44 GMT

←Older revision		Revision as of 07:26, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1re6]] is a 2 chain structure ~~of [[Dynein]]~~ with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA].	+	[[1re6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RE6 OCA].

	==See Also==		==See Also==