1rht - Revision history

OCA at 09:21, 6 December 2023

OCA — Wed, 06 Dec 2023 09:21:17 GMT

←Older revision		Revision as of 09:21, 6 December 2023
Line 1:		Line 1:

	==24-MER RNA HAIRPIN COAT PROTEIN BINDING SITE FOR BACTERIOPHAGE R17 (NMR, MINIMIZED AVERAGE STRUCTURE)==		==24-MER RNA HAIRPIN COAT PROTEIN BINDING SITE FOR BACTERIOPHAGE R17 (NMR, MINIMIZED AVERAGE STRUCTURE)==
-	<StructureSection load='1rht' size='340' side='right'caption='[[1rht~~]], [[NMR_Ensembles_of_Models \| 1 NMR models~~]]' scene=''>	+	<StructureSection load='1rht' size='340' side='right'caption='[[1rht]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RHT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RHT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rht OCA], [https://pdbe.org/1rht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rht RCSB], [https://www.ebi.ac.uk/pdbsum/1rht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rht ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rht OCA], [https://pdbe.org/1rht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rht RCSB], [https://www.ebi.ac.uk/pdbsum/1rht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rht ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 20:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Borer~~, P N~~]]	+	[[Category: Borer PN]]
-	[[Category: Gott~~, J M~~]]	+	[[Category: Gott JM]]
-	[[Category: Lin, Y]]	+	[[Category: Lin Y]]
-	[[Category: Pelczer, I]]	+	[[Category: Pelczer I]]
-	[[Category: Roggenbuck~~, M W~~]]	+	[[Category: Roggenbuck MW]]
-	[[Category: Uhlenbeck~~, O C~~]]	+	[[Category: Uhlenbeck OC]]
-	[[Category: Wang, S]]	+	[[Category: Wang S]]
-	~~[[Category: Bacteriophage r17]]~~	+
-	~~[[Category: Hairpin]]~~	+
-	~~[[Category: Rna~~]]	+

OCA at 10:13, 15 September 2021

OCA — Wed, 15 Sep 2021 10:13:39 GMT

←Older revision		Revision as of 10:13, 15 September 2021
Line 3:		Line 3:
	<StructureSection load='1rht' size='340' side='right'caption='[[1rht]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1rht' size='340' side='right'caption='[[1rht]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1RHT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RHT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1rht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rht OCA], [~~http~~://pdbe.org/1rht PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1rht RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1rht PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1rht ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rht OCA], [https://pdbe.org/1rht PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rht RCSB], [https://www.ebi.ac.uk/pdbsum/1rht PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rht ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:39, 4 December 2019

OCA — Wed, 04 Dec 2019 11:39:20 GMT

←Older revision		Revision as of 11:39, 4 December 2019
Line 1:		Line 1:

	==24-MER RNA HAIRPIN COAT PROTEIN BINDING SITE FOR BACTERIOPHAGE R17 (NMR, MINIMIZED AVERAGE STRUCTURE)==		==24-MER RNA HAIRPIN COAT PROTEIN BINDING SITE FOR BACTERIOPHAGE R17 (NMR, MINIMIZED AVERAGE STRUCTURE)==
-	<StructureSection load='1rht' size='340' side='right' caption='[[1rht]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>	+	<StructureSection load='1rht' size='340' side='right'caption='[[1rht]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RHT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RHT FirstGlance]. <br>
Line 19:		Line 19:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Borer, P N]]		[[Category: Borer, P N]]
	[[Category: Gott, J M]]		[[Category: Gott, J M]]

OCA at 08:03, 28 February 2018

OCA — Wed, 28 Feb 2018 08:03:41 GMT

←Older revision		Revision as of 08:03, 28 February 2018
Line 1:		Line 1:
		+
	==24-MER RNA HAIRPIN COAT PROTEIN BINDING SITE FOR BACTERIOPHAGE R17 (NMR, MINIMIZED AVERAGE STRUCTURE)==		==24-MER RNA HAIRPIN COAT PROTEIN BINDING SITE FOR BACTERIOPHAGE R17 (NMR, MINIMIZED AVERAGE STRUCTURE)==
	<StructureSection load='1rht' size='340' side='right' caption='[[1rht]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>		<StructureSection load='1rht' size='340' side='right' caption='[[1rht]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RHT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RHT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rht OCA], [http://pdbe.org/1rht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rht RCSB], [http://www.ebi.ac.uk/pdbsum/1rht PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rht OCA], [http://pdbe.org/1rht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rht RCSB], [http://www.ebi.ac.uk/pdbsum/1rht PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rht ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 13:59, 11 September 2015

OCA — Fri, 11 Sep 2015 13:59:15 GMT

←Older revision		Revision as of 13:59, 11 September 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RHT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RHT FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rht OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rht RCSB], [http://www.ebi.ac.uk/pdbsum/1rht PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rht OCA], [http://pdbe.org/1rht PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rht RCSB], [http://www.ebi.ac.uk/pdbsum/1rht PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 13:		Line 13:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1rht" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:50, 22 December 2014

OCA — Mon, 22 Dec 2014 05:50:00 GMT

←Older revision		Revision as of 05:50, 22 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RHT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RHT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rht OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rht RCSB], [http://www.ebi.ac.uk/pdbsum/1rht PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rht OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rht RCSB], [http://www.ebi.ac.uk/pdbsum/1rht PDBsum]</span></td></tr>
-	<table>	+	</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 17:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Borer, P N.]]	+	[[Category: Borer, P N]]
-	[[Category: Gott, J M.]]	+	[[Category: Gott, J M]]
-	[[Category: Lin, Y.]]	+	[[Category: Lin, Y]]
-	[[Category: Pelczer, I.]]	+	[[Category: Pelczer, I]]
-	[[Category: Roggenbuck, M W.]]	+	[[Category: Roggenbuck, M W]]
-	[[Category: Uhlenbeck, O C.]]	+	[[Category: Uhlenbeck, O C]]
-	[[Category: Wang, S.]]	+	[[Category: Wang, S]]
	[[Category: Bacteriophage r17]]		[[Category: Bacteriophage r17]]
	[[Category: Hairpin]]		[[Category: Hairpin]]
	[[Category: Rna]]		[[Category: Rna]]

OCA at 07:06, 9 June 2014

OCA — Mon, 09 Jun 2014 07:06:16 GMT

←Older revision		Revision as of 07:06, 9 June 2014
Line 1:		Line 1:
-	[[~~Image~~:1rht.~~png\|left\|200px~~]]	+	==24-MER RNA HAIRPIN COAT PROTEIN BINDING SITE FOR BACTERIOPHAGE R17 (NMR, MINIMIZED AVERAGE STRUCTURE)==
		+	<StructureSection load='1rht' size='340' side='right' caption='[[1rht]], [[NMR_Ensembles_of_Models \| 1 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RHT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rht FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rht OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rht RCSB], [http://www.ebi.ac.uk/pdbsum/1rht PDBsum]</span></td></tr>
		+	<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The three-dimensional conformation of a 24-nucleotide variant of the RNA binding sequence for the coat protein of bacteriophage R17 has been analyzed using NMR, molecular dynamics, and energy minimization. The imino proton spectrum is consistent with base pairing requirements for coat protein binding known from biochemical studies. All 185 of the nonexchangeable protons were assigned using a variety of homonuclear 2D and 3D NMR methods. Measurements of nuclear Overhauser enhancements and two-quantum correlations were made at 500 MHz. New procedures were developed to characterize as many resonances as possible, including deconvolution and path analysis methods. An average of 21 distance constraints per residue were used in molecular dynamics calculations to obtain preliminary folded structures for residues 3-21. The unpaired A8 residue is stacked in the stem, and the entire region from G7 to C15 in the upper stem and loop appears to be flexible. Several of these residues have a large fraction of S-puckered ribose rings, rather than the N-forms characteristic of RNA duplexes. There is considerable variation in the low-energy loop conformations that satisfy the distance constraints at this preliminary level of refinement. The Shine-Dalgarno ribosome binding site is exposed, and only two apparently weak base pairs would have to break for the 16S ribosomal RNA to bind and the ribosome to initiate translation of the replicase gene. Although the loop form must be regarded as tentative, the known interaction sites with the coat protein are easily accessible from the major groove side of the loop.

-	~~{{STRUCTURE_1rht\| PDB=1rht \| SCENE= }}~~	+	Proton NMR and structural features of a 24-nucleotide RNA hairpin.,Borer PN, Lin Y, Wang S, Roggenbuck MW, Gott JM, Uhlenbeck OC, Pelczer I Biochemistry. 1995 May 16;34(19):6488-503. PMID:7756280<ref>PMID:7756280</ref>

-	~~===24-MER RNA HAIRPIN COAT PROTEIN BINDING SITE FOR BACTERIOPHAGE R17 (NMR~~, ~~MINIMIZED AVERAGE STRUCTURE)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_7756280}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1rht]] is~~ a ~~1 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=1RHT OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:007756280<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Borer, P N.]]		[[Category: Borer, P N.]]
	[[Category: Gott, J M.]]		[[Category: Gott, J M.]]

OCA: Protected "1rht" [edit=sysop:move=sysop]

OCA — Tue, 14 Aug 2012 16:15:17 GMT

Protected "1rht" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:15, 14 August 2012

OCA at 16:15, 14 August 2012

OCA — Tue, 14 Aug 2012 16:15:15 GMT

←Older revision		Revision as of 16:15, 14 August 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1rht.png\|left\|200px]]		[[Image:1rht.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1rht", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1rht\| PDB=1rht \| SCENE= }}		{{STRUCTURE_1rht\| PDB=1rht \| SCENE= }}

	===24-MER RNA HAIRPIN COAT PROTEIN BINDING SITE FOR BACTERIOPHAGE R17 (NMR, MINIMIZED AVERAGE STRUCTURE)===		===24-MER RNA HAIRPIN COAT PROTEIN BINDING SITE FOR BACTERIOPHAGE R17 (NMR, MINIMIZED AVERAGE STRUCTURE)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_7756280}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 7756280 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_7756280}}		{{ABSTRACT_PUBMED_7756280}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA].	+	[[1rht]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA].

	==Reference==		==Reference==
-	~~Proton NMR and structural features of a 24-nucleotide RNA hairpin., Borer PN, Lin Y, Wang S, Roggenbuck MW, Gott JM, Uhlenbeck OC, Pelczer I, Biochemistry. 1995 May 16;34(19):6488-503.~~ PMID:~~[http://www.ncbi.nlm.nih.gov~~/~~pubmed~~/~~7756280 7756280]~~	+	<ref group="xtra">PMID:007756280</ref><references group="xtra"/>
	[[Category: Borer, P N.]]		[[Category: Borer, P N.]]
	[[Category: Gott, J M.]]		[[Category: Gott, J M.]]
Line 33:		Line 21:
	[[Category: Bacteriophage r17]]		[[Category: Bacteriophage r17]]
	[[Category: Hairpin]]		[[Category: Hairpin]]
-	[[Category: Nmr]]
	[[Category: Rna]]		[[Category: Rna]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:26:46 2008''

OCA at 04:26, 29 July 2008

OCA — Tue, 29 Jul 2008 04:26:49 GMT

←Older revision		Revision as of 04:26, 29 July 2008
Line 1:		Line 1:
-	[[Image:1rht.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1rht.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1rht\| PDB=1rht \| SCENE= }}		{{STRUCTURE_1rht\| PDB=1rht \| SCENE= }}

-	~~'''~~24-MER RNA HAIRPIN COAT PROTEIN BINDING SITE FOR BACTERIOPHAGE R17 (NMR, MINIMIZED AVERAGE STRUCTURE)~~'''~~	+	===24-MER RNA HAIRPIN COAT PROTEIN BINDING SITE FOR BACTERIOPHAGE R17 (NMR, MINIMIZED AVERAGE STRUCTURE)===


-	~~==Overview==~~	+	<!--
-	~~The three~~-~~dimensional conformation of a 24~~-~~nucleotide variant of the RNA binding sequence for the coat protein of bacteriophage R17 has been analyzed using NMR, molecular dynamics, and energy minimization.~~ The imino proton spectrum is consistent with base pairing requirements for coat protein binding known from biochemical studies. All 185 of the nonexchangeable protons were assigned using a variety of homonuclear 2D and 3D NMR methods. Measurements of nuclear Overhauser enhancements and two-quantum correlations were made at 500 MHz. New procedures were developed to characterize as many resonances as possible, including deconvolution and path analysis methods. An average of 21 distance constraints per residue were used in molecular dynamics calculations to obtain preliminary folded structures for residues 3-21. The unpaired A8 residue is stacked in the stem, ~~and~~ the ~~entire region from G7~~ to ~~C15 in~~ the ~~upper stem and loop~~ appears ~~to be flexible~~. ~~Several of these residues have a large fraction of S-puckered ribose rings~~, ~~rather than the N-forms characteristic of RNA duplexes. There~~ is ~~considerable variation in~~ the ~~low-energy loop conformations that satisfy the distance constraints at this preliminary level of refinement~~. ~~The Shine~~-Dalgarno ribosome binding site is exposed, and only two apparently weak base pairs would have to break for the 16S ribosomal RNA to bind and the ribosome to initiate translation of the replicase gene. Although the loop form must be regarded as tentative, the known interaction sites with the coat protein are easily accessible from the major groove side of the loop.	+	The line below this paragraph, {{ABSTRACT_PUBMED_7756280}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 7756280 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_7756280}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RHT OCA].

	==Reference==		==Reference==
Line 31:		Line 35:
	[[Category: Nmr]]		[[Category: Nmr]]
	[[Category: Rna]]		[[Category: Rna]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3~~ 07:30:55 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:26:46 2008''