1ri2 - Revision history

OCA at 08:23, 14 February 2024

OCA — Wed, 14 Feb 2024 08:23:16 GMT

←Older revision		Revision as of 08:23, 14 February 2024
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	<StructureSection load='1ri2' size='340' side='right'caption='[[1ri2]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1ri2' size='340' side='right'caption='[[1ri2]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ri2]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Enccn Enccn~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1RI2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ri2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RI2 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GTG:~~7~~-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1ri1\|1ri1]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]~~</~~div~~>~~</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ENCCN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ri2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri2 OCA], [https://pdbe.org/1ri2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ri2 RCSB], [https://www.ebi.ac.uk/pdbsum/1ri2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ri2 ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1ri2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri2 OCA], [~~http~~://pdbe.org/1ri2 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ri2 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ri2 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ri2 ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MCES_ENCCU MCES_ENCCU]] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.<ref>PMID:15760890</ref>	+	[https://www.uniprot.org/uniprot/MCES_ENCCU MCES_ENCCU] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.<ref>PMID:15760890</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ri2 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ri2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	A suite of crystal structures is reported for a cellular mRNA cap (guanine-N7) methyltransferase in complex with AdoMet, AdoHcy, and the cap guanylate. Superposition of ligand complexes suggests an in-line mechanism of methyl transfer, albeit without direct contacts between the enzyme and either the N7 atom of guanine (the attacking nucleophile), the methyl carbon of AdoMet, or the sulfur of AdoMet/AdoHcy (the leaving group). The structures indicate that catalysis of cap N7 methylation is accomplished by optimizing proximity and orientation of the substrates, assisted by a favorable electrostatic environment. The enzyme-ligand structures, together with new mutational data, fully account for the biochemical specificity of the cap guanine-N7 methylation reaction, an essential and defining step of eukaryotic mRNA synthesis.
-
-	Structure and mechanism of mRNA cap (guanine-N7) methyltransferase.,Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD Mol Cell. 2004 Jan 16;13(1):77-89. PMID:14731396<ref>PMID:14731396</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1ri2" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[MRNA capping enzyme\|MRNA capping enzyme]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Enccn~~]]	+	[[Category: Encephalitozoon cuniculi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Fabrega, C]]	+	[[Category: Fabrega C]]
-	[[Category: Hausmann, S]]	+	[[Category: Hausmann S]]
-	[[Category: Lima~~, C D~~]]	+	[[Category: Lima CD]]
-	[[Category: Shen, V]]	+	[[Category: Shen V]]
-	[[Category: Shuman, S]]	+	[[Category: Shuman S]]
-	~~[[Category: Cap]]~~	+
-	~~[[Category: M7g]]~~	+
-	~~[[Category: Messenger rna cap]]~~	+
-	~~[[Category: Methyltransferase]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 13:26, 16 December 2020

OCA — Wed, 16 Dec 2020 13:26:52 GMT

←Older revision		Revision as of 13:26, 16 December 2020
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	==Structure and mechanism of mRNA cap (guanine N-7) methyltransferase==		==Structure and mechanism of mRNA cap (guanine N-7) methyltransferase==
-	<StructureSection load='1ri2' size='340' side='right' caption='[[1ri2]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='1ri2' size='340' side='right'caption='[[1ri2]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ri2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enccn Enccn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1RI2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ri2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enccn Enccn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1RI2 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ri1\|1ri1]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ENCCN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ENCCN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ri2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri2 OCA], [http://pdbe.org/1ri2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ri2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ri2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ri2 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ri2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri2 OCA], [http://pdbe.org/1ri2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ri2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ri2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ri2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1ri2" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1ri2" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[MRNA capping enzyme\|MRNA capping enzyme]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Enccn]]		[[Category: Enccn]]
		+	[[Category: Large Structures]]
	[[Category: Fabrega, C]]		[[Category: Fabrega, C]]
	[[Category: Hausmann, S]]		[[Category: Hausmann, S]]

OCA at 08:12, 28 February 2018

OCA — Wed, 28 Feb 2018 08:12:04 GMT

←Older revision		Revision as of 08:12, 28 February 2018
Line 1:		Line 1:
		+
	==Structure and mechanism of mRNA cap (guanine N-7) methyltransferase==		==Structure and mechanism of mRNA cap (guanine N-7) methyltransferase==
	<StructureSection load='1ri2' size='340' side='right' caption='[[1ri2]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1ri2' size='340' side='right' caption='[[1ri2]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ENCCN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ENCCN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ri2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri2 OCA], [http://pdbe.org/1ri2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ri2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ri2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ri2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri2 OCA], [http://pdbe.org/1ri2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ri2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ri2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ri2 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ri/1ri2_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ri/1ri2_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ri2 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:53, 11 September 2015

OCA — Fri, 11 Sep 2015 14:53:17 GMT

←Older revision		Revision as of 14:53, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1ri2' size='340' side='right' caption='[[1ri2]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1ri2' size='340' side='right' caption='[[1ri2]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ri2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Encephalitozoon_cuniculi Encephalitozoon cuniculi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RI2 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ri2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Enccn Enccn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RI2 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ~~Encephalitozoon cuniculi~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 ENCCN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ri2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ri2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ri2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ri2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri2 OCA], [http://pdbe.org/1ri2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ri2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ri2 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ri2" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Encephalitozoon cuniculi~~]]	+	[[Category: Enccn]]
	[[Category: Fabrega, C]]		[[Category: Fabrega, C]]
	[[Category: Hausmann, S]]		[[Category: Hausmann, S]]

OCA at 17:59, 25 December 2014

OCA — Thu, 25 Dec 2014 17:59:51 GMT

←Older revision		Revision as of 17:59, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ri2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RI2 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ri2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RI2 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 Encephalitozoon cuniculi])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 Encephalitozoon cuniculi])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ri2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ri2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ri2 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ri2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ri2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ri2 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MCES_ENCCU MCES_ENCCU]] mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC.<ref>PMID:15760890</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Encephalitozoon cuniculi]]		[[Category: Encephalitozoon cuniculi]]
-	[[Category: Fabrega, C.]]	+	[[Category: Fabrega, C]]
-	[[Category: Hausmann, S.]]	+	[[Category: Hausmann, S]]
-	[[Category: Lima, C D.]]	+	[[Category: Lima, C D]]
-	[[Category: Shen, V.]]	+	[[Category: Shen, V]]
-	[[Category: Shuman, S.]]	+	[[Category: Shuman, S]]
	[[Category: Cap]]		[[Category: Cap]]
	[[Category: M7g]]		[[Category: M7g]]

OCA at 16:42, 29 September 2014

OCA — Mon, 29 Sep 2014 16:42:09 GMT

←Older revision		Revision as of 16:42, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ri2.~~png~~\|~~left~~\|200px]]	+	==Structure and mechanism of mRNA cap (guanine N-7) methyltransferase==
		+	<StructureSection load='1ri2' size='340' side='right' caption='[[1ri2]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ri2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RI2 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GTG:7-METHYL-GUANOSINE-5-TRIPHOSPHATE-5-GUANOSINE'>GTG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ri1\|1ri1]], [[1ri3\|1ri3]], [[1ri4\|1ri4]], [[1ri5\|1ri5]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ECU10_0380 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6035 Encephalitozoon cuniculi])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ri2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ri2 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ri2 RCSB], [http://www.ebi.ac.uk/pdbsum/1ri2 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ri/1ri2_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A suite of crystal structures is reported for a cellular mRNA cap (guanine-N7) methyltransferase in complex with AdoMet, AdoHcy, and the cap guanylate. Superposition of ligand complexes suggests an in-line mechanism of methyl transfer, albeit without direct contacts between the enzyme and either the N7 atom of guanine (the attacking nucleophile), the methyl carbon of AdoMet, or the sulfur of AdoMet/AdoHcy (the leaving group). The structures indicate that catalysis of cap N7 methylation is accomplished by optimizing proximity and orientation of the substrates, assisted by a favorable electrostatic environment. The enzyme-ligand structures, together with new mutational data, fully account for the biochemical specificity of the cap guanine-N7 methylation reaction, an essential and defining step of eukaryotic mRNA synthesis.

-	~~{{STRUCTURE_1ri2\| PDB=1ri2 \| SCENE= }}~~	+	Structure and mechanism of mRNA cap (guanine-N7) methyltransferase.,Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD Mol Cell. 2004 Jan 16;13(1):77-89. PMID:14731396<ref>PMID:14731396</ref>

-	~~===Structure and mechanism~~ of ~~mRNA cap (guanine N-7) methyltransferase===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14731396}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1ri2]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:014731396<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Encephalitozoon cuniculi]]		[[Category: Encephalitozoon cuniculi]]
	[[Category: Fabrega, C.]]		[[Category: Fabrega, C.]]

OCA: Protected "1ri2" [edit=sysop:move=sysop]

OCA — Fri, 04 Jan 2013 10:49:24 GMT

Protected "1ri2" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:49, 4 January 2013

OCA at 10:49, 4 January 2013

OCA — Fri, 04 Jan 2013 10:49:22 GMT

←Older revision		Revision as of 10:49, 4 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1ri2.png\|left\|200px]]		[[Image:1ri2.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1ri2", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1ri2\| PDB=1ri2 \| SCENE= }}		{{STRUCTURE_1ri2\| PDB=1ri2 \| SCENE= }}

	===Structure and mechanism of mRNA cap (guanine N-7) methyltransferase===		===Structure and mechanism of mRNA cap (guanine N-7) methyltransferase===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_14731396}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 14731396 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_14731396}}		{{ABSTRACT_PUBMED_14731396}}

	==About this Structure==		==About this Structure==
-	~~1RI2~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA].	+	[[1ri2]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~14731396~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014731396</ref><references group="xtra"/>
	[[Category: Encephalitozoon cuniculi]]		[[Category: Encephalitozoon cuniculi]]
	[[Category: Fabrega, C.]]		[[Category: Fabrega, C.]]
Line 35:		Line 23:
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Rna]]		[[Category: Rna]]
-		+	[[Category: Transferase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 00:54:01 2009''~~	+

OCA at 22:54, 17 February 2009

OCA — Tue, 17 Feb 2009 22:54:01 GMT

←Older revision		Revision as of 22:54, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1RI2 is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA].	+	1RI2 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Encephalitozoon_cuniculi Encephalitozoon cuniculi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RI2 OCA].

	==Reference==		==Reference==
-	~~Structure and mechanism of mRNA cap (guanine-N7) methyltransferase., Fabrega C, Hausmann S, Shen V, Shuman S, Lima CD, Mol Cell. 2004 Jan 16;13(1):77-89.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/14731396 14731396]~~	+	<ref group="xtra">PMID:14731396</ref><references group="xtra"/>
	[[Category: Encephalitozoon cuniculi]]		[[Category: Encephalitozoon cuniculi]]
-	[[Category: Single protein]]
	[[Category: Fabrega, C.]]		[[Category: Fabrega, C.]]
	[[Category: Hausmann, S.]]		[[Category: Hausmann, S.]]
Line 37:		Line 36:
	[[Category: Rna]]		[[Category: Rna]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 08~~:20:~~17 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 00:54:01 2009''

OCA at 05:20, 29 July 2008

OCA — Tue, 29 Jul 2008 05:20:19 GMT

←Older revision		Revision as of 05:20, 29 July 2008
Line 1:		Line 1:
-	[[Image:1ri2.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1ri2.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1ri2\| PDB=1ri2 \| SCENE= }}		{{STRUCTURE_1ri2\| PDB=1ri2 \| SCENE= }}

-	~~'''~~Structure and mechanism of mRNA cap (guanine N-7) methyltransferase~~'''~~	+	===Structure and mechanism of mRNA cap (guanine N-7) methyltransferase===


-	~~==Overview==~~	+	<!--
-	~~A suite of crystal structures is reported for a cellular mRNA cap (guanine~~-~~N7) methyltransferase in complex with AdoMet, AdoHcy, and the cap guanylate. Superposition of ligand complexes suggests an in~~-line ~~mechanism of methyl transfer~~, ~~albeit without direct contacts between~~ the ~~enzyme and either~~ the ~~N7 atom of guanine~~ (~~the attacking nucleophile~~), ~~the methyl carbon of AdoMet, or the sulfur of AdoMet/AdoHcy (the leaving group). The structures indicate that catalysis of cap N7 methylation~~ is ~~accomplished by optimizing proximity and orientation of~~ the ~~substrates, assisted by a favorable electrostatic environment~~. ~~The enzyme~~-~~ligand structures, together with new mutational data, fully account for the biochemical specificity of the cap guanine~~-~~N7 methylation reaction, an essential and defining step of eukaryotic mRNA synthesis.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_14731396}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 14731396 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_14731396}}

	==About this Structure==		==About this Structure==
Line 32:		Line 36:
	[[Category: Methyltransferase]]		[[Category: Methyltransferase]]
	[[Category: Rna]]		[[Category: Rna]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 07~~:31:27 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:20:17 2008''