1rz4 - Revision history

OCA at 08:27, 1 May 2024

OCA — Wed, 01 May 2024 08:27:51 GMT

←Older revision		Revision as of 08:27, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='1rz4' size='340' side='right'caption='[[1rz4]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1rz4' size='340' side='right'caption='[[1rz4]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1rz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RZ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1rz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RZ4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz4 OCA], [https://pdbe.org/1rz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rz4 RCSB], [https://www.ebi.ac.uk/pdbsum/1rz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rz4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz4 OCA], [https://pdbe.org/1rz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rz4 RCSB], [https://www.ebi.ac.uk/pdbsum/1rz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rz4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/EIF3K_HUMAN EIF3K_HUMAN]] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.	+	[https://www.uniprot.org/uniprot/EIF3K_HUMAN EIF3K_HUMAN] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rz4 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rz4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	eIF3k, the smallest subunit of eukaryotic initiation factor 3 (eIF3), interacts with several other subunits of eIF3 and the 40 S ribosomal subunit. eIF3k is conserved among high eukaryotes, including mammals, insects, and plants, and it is ubiquitously expressed in human tissues. Interestingly, eIF3k does not exist in some species of yeast. Thus, eIF3k may play a unique regulatory role in higher organisms. Here we report the crystal structure of human eIF3k, the first high-resolution structure of an eIF3 component. This novel structure contains two distinct domains, a HEAT (named for Huntington, elongation factor 3, A subunit of protein phosphatase 2A, target of rapamycin) repeat-like HAM (HEAT analogous motif) domain and a winged-helix-like WH domain. Through structural comparison and sequence conservation analysis, we show that eIF3k has three putative protein-binding surfaces and has potential RNA binding activity. The structure provides key information for understanding the structure and function of the eIF3 complex.
-
-	Crystal structure of human eIF3k, the first structure of eIF3 subunits.,Wei Z, Zhang P, Zhou Z, Cheng Z, Wan M, Gong W J Biol Chem. 2004 Aug 13;279(33):34983-90. Epub 2004 Jun 4. PMID:15180986<ref>PMID:15180986</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1rz4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Eukaryotic initiation factor 3D structures\|Eukaryotic initiation factor 3D structures]]		*[[Eukaryotic initiation factor 3D structures\|Eukaryotic initiation factor 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Gong, W]]	+	[[Category: Gong W]]
-	[[Category: Wei, Z]]	+	[[Category: Wei Z]]
-	[[Category: Zhang, P]]	+	[[Category: Zhang P]]
-	[[Category: Zhou, Z]]	+	[[Category: Zhou Z]]
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: Heat analogous motif]]~~	+
-	~~[[Category: Winged-helix~~]]	+

OCA at 07:24, 22 September 2021

OCA — Wed, 22 Sep 2021 07:24:26 GMT

←Older revision		Revision as of 07:24, 22 September 2021
Line 3:		Line 3:
	<StructureSection load='1rz4' size='340' side='right'caption='[[1rz4]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1rz4' size='340' side='right'caption='[[1rz4]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1rz4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1RZ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1rz4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1RZ4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1rz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz4 OCA], [~~http~~://pdbe.org/1rz4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1rz4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1rz4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1rz4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1rz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz4 OCA], [https://pdbe.org/1rz4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1rz4 RCSB], [https://www.ebi.ac.uk/pdbsum/1rz4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1rz4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/EIF3K_HUMAN EIF3K_HUMAN]] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.	+	[[https://www.uniprot.org/uniprot/EIF3K_HUMAN EIF3K_HUMAN]] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 19:32, 11 December 2019

OCA — Wed, 11 Dec 2019 19:32:33 GMT

←Older revision		Revision as of 19:32, 11 December 2019
Line 1:		Line 1:

	==Crystal Structure of Human eIF3k==		==Crystal Structure of Human eIF3k==
-	<StructureSection load='1rz4' size='340' side='right' caption='[[1rz4]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1rz4' size='340' side='right'caption='[[1rz4]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RZ4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RZ4 FirstGlance]. <br>
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Eukaryotic initiation factor\|Eukaryotic initiation factor]]	+	*[[Eukaryotic initiation factor 3D structures\|Eukaryotic initiation factor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Gong, W]]		[[Category: Gong, W]]
	[[Category: Wei, Z]]		[[Category: Wei, Z]]

OCA at 07:03, 8 March 2018

OCA — Thu, 08 Mar 2018 07:03:10 GMT

←Older revision		Revision as of 07:03, 8 March 2018
Line 1:		Line 1:
		+
	==Crystal Structure of Human eIF3k==		==Crystal Structure of Human eIF3k==
	<StructureSection load='1rz4' size='340' side='right' caption='[[1rz4]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1rz4' size='340' side='right' caption='[[1rz4]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz4 OCA], [http://pdbe.org/1rz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rz4 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz4 OCA], [http://pdbe.org/1rz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rz4 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1rz4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rz/1rz4_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rz/1rz4_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1rz4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:22, 11 September 2015

OCA — Fri, 11 Sep 2015 22:22:22 GMT

←Older revision		Revision as of 22:22, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1rz4' size='340' side='right' caption='[[1rz4]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1rz4' size='340' side='right' caption='[[1rz4]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1rz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RZ4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1rz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RZ4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rz4 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz4 OCA], [http://pdbe.org/1rz4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1rz4 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz4 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~IF3C_HUMAN IF3C_HUMAN~~]] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.~~[HAMAP-Rule:MF_03010]~~	+	[[http://www.uniprot.org/uniprot/EIF3K_HUMAN EIF3K_HUMAN]] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1rz4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Gong, W]]		[[Category: Gong, W]]
	[[Category: Wei, Z]]		[[Category: Wei, Z]]

OCA at 20:15, 25 December 2014

OCA — Thu, 25 Dec 2014 20:15:59 GMT

←Older revision		Revision as of 20:15, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1rz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RZ4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1rz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RZ4 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rz4 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rz4 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz4 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IF3C_HUMAN IF3C_HUMAN]] Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.[HAMAP-Rule:MF_03010]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Gong, W.]]	+	[[Category: Gong, W]]
-	[[Category: Wei, Z.]]	+	[[Category: Wei, Z]]
-	[[Category: Zhang, P.]]	+	[[Category: Zhang, P]]
-	[[Category: Zhou, Z.]]	+	[[Category: Zhou, Z]]
	[[Category: Biosynthetic protein]]		[[Category: Biosynthetic protein]]
	[[Category: Heat analogous motif]]		[[Category: Heat analogous motif]]
	[[Category: Winged-helix]]		[[Category: Winged-helix]]

OCA at 21:12, 28 September 2014

OCA — Sun, 28 Sep 2014 21:12:10 GMT

←Older revision		Revision as of 21:12, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1rz4.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of Human eIF3k==
		+	<StructureSection load='1rz4' size='340' side='right' caption='[[1rz4]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1rz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1RZ4 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1rz4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1rz4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1rz4 RCSB], [http://www.ebi.ac.uk/pdbsum/1rz4 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rz/1rz4_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	eIF3k, the smallest subunit of eukaryotic initiation factor 3 (eIF3), interacts with several other subunits of eIF3 and the 40 S ribosomal subunit. eIF3k is conserved among high eukaryotes, including mammals, insects, and plants, and it is ubiquitously expressed in human tissues. Interestingly, eIF3k does not exist in some species of yeast. Thus, eIF3k may play a unique regulatory role in higher organisms. Here we report the crystal structure of human eIF3k, the first high-resolution structure of an eIF3 component. This novel structure contains two distinct domains, a HEAT (named for Huntington, elongation factor 3, A subunit of protein phosphatase 2A, target of rapamycin) repeat-like HAM (HEAT analogous motif) domain and a winged-helix-like WH domain. Through structural comparison and sequence conservation analysis, we show that eIF3k has three putative protein-binding surfaces and has potential RNA binding activity. The structure provides key information for understanding the structure and function of the eIF3 complex.

-	~~{{STRUCTURE_1rz4\| PDB=1rz4 \| SCENE= }}~~	+	Crystal structure of human eIF3k, the first structure of eIF3 subunits.,Wei Z, Zhang P, Zhou Z, Cheng Z, Wan M, Gong W J Biol Chem. 2004 Aug 13;279(33):34983-90. Epub 2004 Jun 4. PMID:15180986<ref>PMID:15180986</ref>

-	~~===Crystal Structure~~ of ~~Human eIF3k===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15180986}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1rz4]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Homo_sapiens Homo sapiens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA].~~	+

	==See Also==		==See Also==
	*[[Eukaryotic initiation factor\|Eukaryotic initiation factor]]		*[[Eukaryotic initiation factor\|Eukaryotic initiation factor]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015180986<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Gong, W.]]		[[Category: Gong, W.]]

OCA: Protected "1rz4" [edit=sysop:move=sysop]

OCA — Sat, 20 Oct 2012 21:00:23 GMT

Protected "1rz4" [edit=sysop:move=sysop]

←Older revision

Revision as of 21:00, 20 October 2012

OCA at 21:00, 20 October 2012

OCA — Sat, 20 Oct 2012 21:00:21 GMT

←Older revision		Revision as of 21:00, 20 October 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1rz4.png\|left\|200px]]		[[Image:1rz4.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1rz4", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1rz4\| PDB=1rz4 \| SCENE= }}		{{STRUCTURE_1rz4\| PDB=1rz4 \| SCENE= }}

	===Crystal Structure of Human eIF3k===		===Crystal Structure of Human eIF3k===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15180986}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15180986 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15180986}}		{{ABSTRACT_PUBMED_15180986}}

	==About this Structure==		==About this Structure==
-	~~1RZ4~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA].	+	[[1rz4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA].
		+
		+	==See Also==
		+	*[[Eukaryotic initiation factor\|Eukaryotic initiation factor]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15180986~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015180986</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Gong, W.]]		[[Category: Gong, W.]]
Line 29:		Line 20:
	[[Category: Zhang, P.]]		[[Category: Zhang, P.]]
	[[Category: Zhou, Z.]]		[[Category: Zhou, Z.]]
		+	[[Category: Biosynthetic protein]]
	[[Category: Heat analogous motif]]		[[Category: Heat analogous motif]]
	[[Category: Winged-helix]]		[[Category: Winged-helix]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:52:58 2009''

OCA at 03:52, 18 February 2009

OCA — Wed, 18 Feb 2009 03:52:59 GMT

←Older revision		Revision as of 03:52, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1RZ4 is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA].	+	1RZ4 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RZ4 OCA].

	==Reference==		==Reference==
-	~~Crystal structure of human eIF3k, the first structure of eIF3 subunits., Wei Z, Zhang P, Zhou Z, Cheng Z, Wan M, Gong W, J Biol Chem. 2004 Aug 13;279(33):34983-90. Epub 2004 Jun 4.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15180986 15180986]~~	+	<ref group="xtra">PMID:15180986</ref><references group="xtra"/>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Single protein]]
	[[Category: Gong, W.]]		[[Category: Gong, W.]]
	[[Category: Wei, Z.]]		[[Category: Wei, Z.]]
Line 33:		Line 32:
	[[Category: Winged-helix]]		[[Category: Winged-helix]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 12~~:37:~~14 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 05:52:58 2009''