1s4u - Revision history

OCA at 13:29, 13 March 2024

OCA — Wed, 13 Mar 2024 13:29:43 GMT

←Older revision		Revision as of 13:29, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='1s4u' size='340' side='right'caption='[[1s4u]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1s4u' size='340' side='right'caption='[[1s4u]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1s4u]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1S4U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1s4u]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1S4U FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~SKI8~~, ~~YGL213C (~~[~~http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=4932 ATCC 18824])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1s4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4u OCA], [~~http~~://pdbe.org/1s4u PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1s4u RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1s4u PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1s4u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1s4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4u OCA], [https://pdbe.org/1s4u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1s4u RCSB], [https://www.ebi.ac.uk/pdbsum/1s4u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1s4u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SKI8_YEAST SKI8_YEAST]] Involved in double-strand break (DSB) formation during meiotic recombination through stabilization of SPO11 association with meiotic chromosome and helping SPO11 to recruit other DSB proteins like REC102 and REC104 to meiotic chromosomes. Also component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for controlling the propagation of M double-stranded RNA (dsRNA) and thus for preventing virus-induced cytopathology.<ref>PMID:6371496</ref> <ref>PMID:3029964</ref> <ref>PMID:7739557</ref> <ref>PMID:9258672</ref> <ref>PMID:9482746</ref> <ref>PMID:10744028</ref> <ref>PMID:11532933</ref> <ref>PMID:11720286</ref> <ref>PMID:14671320</ref> <ref>PMID:15044957</ref> <ref>PMID:14992724</ref> <ref>PMID:16816949</ref>	+	[https://www.uniprot.org/uniprot/SKI8_YEAST SKI8_YEAST] Involved in double-strand break (DSB) formation during meiotic recombination through stabilization of SPO11 association with meiotic chromosome and helping SPO11 to recruit other DSB proteins like REC102 and REC104 to meiotic chromosomes. Also component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for controlling the propagation of M double-stranded RNA (dsRNA) and thus for preventing virus-induced cytopathology.<ref>PMID:6371496</ref> <ref>PMID:3029964</ref> <ref>PMID:7739557</ref> <ref>PMID:9258672</ref> <ref>PMID:9482746</ref> <ref>PMID:10744028</ref> <ref>PMID:11532933</ref> <ref>PMID:11720286</ref> <ref>PMID:14671320</ref> <ref>PMID:15044957</ref> <ref>PMID:14992724</ref> <ref>PMID:16816949</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s4u ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s4u ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Ski8p is a WD-repeat protein with an essential role for the Ski complex assembly in an exosome-dependent 3'-to-5' mRNA decay. In addition, Ski8p is involved in meiotic recombination by interacting with Spo11p protein. We have determined the crystal structure of Ski8p from Saccharomyces cerevisiae at 2.2 A resolution. The structure reveals that Ski8p folds into a seven-bladed beta propeller. Mapping sequence conservation and hydrophobicities of amino acids on the molecular surface of Ski8p reveals a prominent site on the top surface of the beta propeller, which is most likely involved in mediating interactions of Ski8p with Ski3p and Spo11p. Mutagenesis combined with yeast two-hybrid and GST pull-down assays identified the top surface of the beta propeller as being required for Ski8p binding to Ski3p and Spo11p. The functional implications for Ski8p function in both mRNA decay and meiotic recombination are discussed.
-
-	Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination.,Cheng Z, Liu Y, Wang C, Parker R, Song H Protein Sci. 2004 Oct;13(10):2673-84. Epub 2004 Aug 31. PMID:15340168<ref>PMID:15340168</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1s4u" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Cheng, Z~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: ~~Song, H~~]]	+	[[Category: Cheng Z]]
-	[[Category: ~~Antiviral protein]]~~	+	[[Category: Song H]]
-	~~[[Category: Wd repeat~~]]	+

OCA at 13:40, 16 December 2020

OCA — Wed, 16 Dec 2020 13:40:23 GMT

←Older revision		Revision as of 13:40, 16 December 2020
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	==Crystal Structure analysis of the beta-propeller protein Ski8p==		==Crystal Structure analysis of the beta-propeller protein Ski8p==
-	<StructureSection load='1s4u' size='340' side='right' caption='[[1s4u]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1s4u' size='340' side='right'caption='[[1s4u]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1s4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1S4U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1s4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1S4U FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SKI8, YGL213C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SKI8, YGL213C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1s4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4u OCA], [http://pdbe.org/1s4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1s4u RCSB], [http://www.ebi.ac.uk/pdbsum/1s4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1s4u ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1s4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4u OCA], [http://pdbe.org/1s4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1s4u RCSB], [http://www.ebi.ac.uk/pdbsum/1s4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1s4u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Cheng, Z]]		[[Category: Cheng, Z]]
	[[Category: Song, H]]		[[Category: Song, H]]
	[[Category: Antiviral protein]]		[[Category: Antiviral protein]]
	[[Category: Wd repeat]]		[[Category: Wd repeat]]

OCA at 06:56, 8 March 2018

OCA — Thu, 08 Mar 2018 06:56:34 GMT

←Older revision		Revision as of 06:56, 8 March 2018
Line 1:		Line 1:
		+
	==Crystal Structure analysis of the beta-propeller protein Ski8p==		==Crystal Structure analysis of the beta-propeller protein Ski8p==
	<StructureSection load='1s4u' size='340' side='right' caption='[[1s4u]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1s4u' size='340' side='right' caption='[[1s4u]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1s4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S4U FirstGlance]. <br>		<table><tr><td colspan='2'>[[1s4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S4U FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SKI8, YGL213C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SKI8, YGL213C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4u OCA], [http://pdbe.org/1s4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1s4u RCSB], [http://www.ebi.ac.uk/pdbsum/1s4u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4u OCA], [http://pdbe.org/1s4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1s4u RCSB], [http://www.ebi.ac.uk/pdbsum/1s4u PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1s4u ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s4/1s4u_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s4/1s4u_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:04, 7 February 2016

OCA — Sun, 07 Feb 2016 11:04:31 GMT

←Older revision		Revision as of 11:04, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1s4u ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:53, 10 September 2015

OCA — Thu, 10 Sep 2015 00:53:34 GMT

←Older revision		Revision as of 00:53, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1s4u' size='340' side='right' caption='[[1s4u]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1s4u' size='340' side='right' caption='[[1s4u]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1s4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S4U FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1s4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S4U FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SKI8, YGL213C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SKI8, YGL213C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s4u RCSB], [http://www.ebi.ac.uk/pdbsum/1s4u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4u OCA], [http://pdbe.org/1s4u PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1s4u RCSB], [http://www.ebi.ac.uk/pdbsum/1s4u PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1s4u" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Cheng, Z]]		[[Category: Cheng, Z]]
	[[Category: Song, H]]		[[Category: Song, H]]
	[[Category: Antiviral protein]]		[[Category: Antiviral protein]]
	[[Category: Wd repeat]]		[[Category: Wd repeat]]

OCA at 14:36, 24 December 2014

OCA — Wed, 24 Dec 2014 14:36:31 GMT

←Older revision		Revision as of 14:36, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1s4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S4U FirstGlance]. <br>		<table><tr><td colspan='2'>[[1s4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S4U FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SKI8, YGL213C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SKI8, YGL213C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s4u RCSB], [http://www.ebi.ac.uk/pdbsum/1s4u PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s4u RCSB], [http://www.ebi.ac.uk/pdbsum/1s4u PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SKI8_YEAST SKI8_YEAST]] Involved in double-strand break (DSB) formation during meiotic recombination through stabilization of SPO11 association with meiotic chromosome and helping SPO11 to recruit other DSB proteins like REC102 and REC104 to meiotic chromosomes. Also component of the SKI complex involved in 3'-mRNA degradation pathway. Represses dsRNA virus propagation by specifically blocking translation of viral mRNAs, perhaps recognizing the absence of CAP or poly(A). Essential for controlling the propagation of M double-stranded RNA (dsRNA) and thus for preventing virus-induced cytopathology.<ref>PMID:6371496</ref> <ref>PMID:3029964</ref> <ref>PMID:7739557</ref> <ref>PMID:9258672</ref> <ref>PMID:9482746</ref> <ref>PMID:10744028</ref> <ref>PMID:11532933</ref> <ref>PMID:11720286</ref> <ref>PMID:14671320</ref> <ref>PMID:15044957</ref> <ref>PMID:14992724</ref> <ref>PMID:16816949</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Cheng, Z.]]	+	[[Category: Cheng, Z]]
-	[[Category: Song, H.]]	+	[[Category: Song, H]]
	[[Category: Antiviral protein]]		[[Category: Antiviral protein]]
	[[Category: Wd repeat]]		[[Category: Wd repeat]]

OCA at 16:02, 29 September 2014

OCA — Mon, 29 Sep 2014 16:02:04 GMT

←Older revision		Revision as of 16:02, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1s4u.~~png\|left~~\|200px]]	+	==Crystal Structure analysis of the beta-propeller protein Ski8p==
		+	<StructureSection load='1s4u' size='340' side='right' caption='[[1s4u]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1s4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1S4U FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SKI8, YGL213C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1s4u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1s4u OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1s4u RCSB], [http://www.ebi.ac.uk/pdbsum/1s4u PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/s4/1s4u_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ski8p is a WD-repeat protein with an essential role for the Ski complex assembly in an exosome-dependent 3'-to-5' mRNA decay. In addition, Ski8p is involved in meiotic recombination by interacting with Spo11p protein. We have determined the crystal structure of Ski8p from Saccharomyces cerevisiae at 2.2 A resolution. The structure reveals that Ski8p folds into a seven-bladed beta propeller. Mapping sequence conservation and hydrophobicities of amino acids on the molecular surface of Ski8p reveals a prominent site on the top surface of the beta propeller, which is most likely involved in mediating interactions of Ski8p with Ski3p and Spo11p. Mutagenesis combined with yeast two-hybrid and GST pull-down assays identified the top surface of the beta propeller as being required for Ski8p binding to Ski3p and Spo11p. The functional implications for Ski8p function in both mRNA decay and meiotic recombination are discussed.

-	~~{{STRUCTURE_1s4u\| PDB=1s4u \| SCENE= }}~~	+	Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination.,Cheng Z, Liu Y, Wang C, Parker R, Song H Protein Sci. 2004 Oct;13(10):2673-84. Epub 2004 Aug 31. PMID:15340168<ref>PMID:15340168</ref>

-	~~===Crystal Structure analysis~~ of the ~~beta-propeller protein Ski8p===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15340168}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1s4u]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015340168<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Cheng, Z.]]		[[Category: Cheng, Z.]]

OCA: Protected "1s4u" [edit=sysop:move=sysop]

OCA — Fri, 04 Jan 2013 10:25:42 GMT

Protected "1s4u" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:25, 4 January 2013

OCA at 10:25, 4 January 2013

OCA — Fri, 04 Jan 2013 10:25:40 GMT

←Older revision		Revision as of 10:25, 4 January 2013
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-	{{Seed}}
	[[Image:1s4u.png\|left\|200px]]		[[Image:1s4u.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1s4u\| PDB=1s4u \| SCENE= }}		{{STRUCTURE_1s4u\| PDB=1s4u \| SCENE= }}

	===Crystal Structure analysis of the beta-propeller protein Ski8p===		===Crystal Structure analysis of the beta-propeller protein Ski8p===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15340168}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15340168 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_15340168}}		{{ABSTRACT_PUBMED_15340168}}

	==About this Structure==		==About this Structure==
-	~~1S4U~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA].	+	[[1s4u]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15340168~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015340168</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Cheng, Z.]]		[[Category: Cheng, Z.]]
	[[Category: Song, H.]]		[[Category: Song, H.]]
		+	[[Category: Antiviral protein]]
	[[Category: Wd repeat]]		[[Category: Wd repeat]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 13:22:41 2009''

OCA at 11:22, 16 February 2009

OCA — Mon, 16 Feb 2009 11:22:41 GMT

←Older revision		Revision as of 11:22, 16 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1S4U is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA].	+	1S4U is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1S4U OCA].

	==Reference==		==Reference==
-	Crystal structure of Ski8p, a WD-repeat protein with dual roles in mRNA metabolism and meiotic recombination., Cheng Z, Liu Y, Wang C, Parker R, Song H, Protein Sci. 2004 Oct;13(10):2673-84. Epub 2004 Aug 31. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15340168 15340168]~~	+	<ref group="xtra">PMID:15340168</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Single protein]]
	[[Category: Cheng, Z.]]		[[Category: Cheng, Z.]]
	[[Category: Song, H.]]		[[Category: Song, H.]]
	[[Category: Wd repeat]]		[[Category: Wd repeat]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27 15~~:47:~~18 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 13:22:41 2009''