1sca - Revision history

OCA at 08:29, 14 February 2024

OCA — Wed, 14 Feb 2024 08:29:34 GMT

←Older revision		Revision as of 08:29, 14 February 2024
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	<StructureSection load='1sca' size='340' side='right'caption='[[1sca]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1sca' size='340' side='right'caption='[[1sca]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SCA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SCA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"><~~span class~~='~~plainlinks~~'>~~[https:~~/~~/en.wikipedia.org/wiki/Subtilisin Subtilisin]~~, ~~with EC number [https~~:~~//www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62]~~ </~~span~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sca OCA], [https://pdbe.org/1sca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sca RCSB], [https://www.ebi.ac.uk/pdbsum/1sca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sca ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sca OCA], [https://pdbe.org/1sca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sca RCSB], [https://www.ebi.ac.uk/pdbsum/1sca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sca ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~SUBT_BACLI SUBT_BACLI]~~] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.	+	[https://www.uniprot.org/uniprot/SUBC_BACLI SUBC_BACLI] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides (Ref.4, PubMed:11109488). Shows high specificity for aromatic and hydrophobic amino acids in the P1 substrate position (PubMed:11109488). May play an important role in the degradation of feather keratin (PubMed:11109488).<ref>PMID:11109488</ref> <ref>PMID:4967581</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sca ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sca ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structure of the serine protease subtilisin Carlsberg in anhydrous acetonitrile was determined at 2.3 A resolution. It was found to be essentially identical to the three-dimensional structure of the enzyme in water; the differences observed were smaller than those between two independently determined structures in aqueous solution. The hydrogen bond system of the catalytic triad is intact in acetonitrile. The majority (99 of 119) of enzyme-bound, structural water molecules have such a great affinity to subtilisin that they are not displaced even in anhydrous acetonitrile. Of the 12 enzyme-bound acetonitrile molecules, 4 displace water molecules and 8 bind where no water had been observed before. One-third of all subtilisin-bound acetonitrile molecules reside in the active center, occupying the same region (P1, P2, and P3 binding sites) as the specific protein inhibitor eglin c.
-
-	Enzyme crystal structure in a neat organic solvent.,Fitzpatrick PA, Steinmetz AC, Ringe D, Klibanov AM Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8653-7. PMID:8378343<ref>PMID:8378343</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1sca" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 27:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Clostridium licheniforme weigmann 1898~~]]	+	[[Category: Bacillus licheniformis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Subtilisin]]~~	+	[[Category: Fitzpatrick PA]]
-	[[Category: Fitzpatrick~~, P A~~]]	+	[[Category: Klibanov AM]]
-	[[Category: Klibanov~~, A M~~]]	+	[[Category: Ringe D]]
-	[[Category: Ringe, D]]	+	[[Category: Steinmetz ACU]]
-	[[Category: Steinmetz~~, A C.U]]~~	+
-	~~[[Category: Serine protease~~]]	+

OCA at 07:29, 22 September 2021

OCA — Wed, 22 Sep 2021 07:29:05 GMT

←Older revision		Revision as of 07:29, 22 September 2021
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	<StructureSection load='1sca' size='340' side='right'caption='[[1sca]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1sca' size='340' side='right'caption='[[1sca]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sca]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1SCA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SCA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1sca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sca OCA], [~~http~~://pdbe.org/1sca PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1sca RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1sca PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1sca ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sca OCA], [https://pdbe.org/1sca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sca RCSB], [https://www.ebi.ac.uk/pdbsum/1sca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sca ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SUBT_BACLI SUBT_BACLI]] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.	+	[[https://www.uniprot.org/uniprot/SUBT_BACLI SUBT_BACLI]] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Subtilisin\|Subtilisin]]	+	*[[Subtilisin 3D structures\|Subtilisin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:23, 4 December 2019

OCA — Wed, 04 Dec 2019 11:23:26 GMT

←Older revision		Revision as of 11:23, 4 December 2019
Line 1:		Line 1:

	==ENZYME CRYSTAL STRUCTURE IN A NEAT ORGANIC SOLVENT==		==ENZYME CRYSTAL STRUCTURE IN A NEAT ORGANIC SOLVENT==
-	<StructureSection load='1sca' size='340' side='right' caption='[[1sca]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1sca' size='340' side='right'caption='[[1sca]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1sca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SCA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1sca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SCA FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sc/1sca_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sc/1sca_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1sca" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1sca" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Subtilisin\|Subtilisin]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Clostridium licheniforme weigmann 1898]]		[[Category: Clostridium licheniforme weigmann 1898]]
		+	[[Category: Large Structures]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
	[[Category: Fitzpatrick, P A]]		[[Category: Fitzpatrick, P A]]

OCA at 06:56, 29 November 2017

OCA — Wed, 29 Nov 2017 06:56:24 GMT

←Older revision		Revision as of 06:56, 29 November 2017
Line 1:		Line 1:
		+
	==ENZYME CRYSTAL STRUCTURE IN A NEAT ORGANIC SOLVENT==		==ENZYME CRYSTAL STRUCTURE IN A NEAT ORGANIC SOLVENT==
	<StructureSection load='1sca' size='340' side='right' caption='[[1sca]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1sca' size='340' side='right' caption='[[1sca]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sca OCA], [http://pdbe.org/1sca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sca RCSB], [http://www.ebi.ac.uk/pdbsum/1sca PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sca OCA], [http://pdbe.org/1sca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sca RCSB], [http://www.ebi.ac.uk/pdbsum/1sca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sca ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</div>		</div>
	<div class="pdbe-citations 1sca" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1sca" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Subtilisin\|Subtilisin]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:48, 7 February 2016

OCA — Sun, 07 Feb 2016 14:48:39 GMT

←Older revision		Revision as of 14:48, 7 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sca ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:31, 10 September 2015

OCA — Thu, 10 Sep 2015 02:31:20 GMT

←Older revision		Revision as of 02:31, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1sca' size='340' side='right' caption='[[1sca]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1sca' size='340' side='right' caption='[[1sca]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_licheniformis Bacillus licheniformis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SCA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"clostridium_licheniforme"_weigmann_1898 "clostridium licheniforme" weigmann 1898]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SCA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sca OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sca RCSB], [http://www.ebi.ac.uk/pdbsum/1sca PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sca OCA], [http://pdbe.org/1sca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sca RCSB], [http://www.ebi.ac.uk/pdbsum/1sca PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1sca" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus licheniformis~~]]	+	[[Category: Clostridium licheniforme weigmann 1898]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
	[[Category: Fitzpatrick, P A]]		[[Category: Fitzpatrick, P A]]

OCA at 16:01, 24 December 2014

OCA — Wed, 24 Dec 2014 16:01:34 GMT

←Older revision		Revision as of 16:01, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1sca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SCA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1sca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SCA FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sca OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sca RCSB], [http://www.ebi.ac.uk/pdbsum/1sca PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sca OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sca RCSB], [http://www.ebi.ac.uk/pdbsum/1sca PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SUBT_BACLI SUBT_BACLI]] Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 36:
	[[Category: Bacillus licheniformis]]		[[Category: Bacillus licheniformis]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]
-	[[Category: Fitzpatrick, P A.]]	+	[[Category: Fitzpatrick, P A]]
-	[[Category: Klibanov, A M.]]	+	[[Category: Klibanov, A M]]
-	[[Category: Ringe, D.]]	+	[[Category: Ringe, D]]
-	[[Category: Steinmetz, A C.U.]]	+	[[Category: Steinmetz, A C.U]]
	[[Category: Serine protease]]		[[Category: Serine protease]]

OCA at 23:20, 28 September 2014

OCA — Sun, 28 Sep 2014 23:20:29 GMT

←Older revision		Revision as of 23:20, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1sca.~~png\|left~~\|200px]]	+	==ENZYME CRYSTAL STRUCTURE IN A NEAT ORGANIC SOLVENT==
		+	<StructureSection load='1sca' size='340' side='right' caption='[[1sca]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1sca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SCA FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene><br>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sca OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sca RCSB], [http://www.ebi.ac.uk/pdbsum/1sca PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sc/1sca_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the serine protease subtilisin Carlsberg in anhydrous acetonitrile was determined at 2.3 A resolution. It was found to be essentially identical to the three-dimensional structure of the enzyme in water; the differences observed were smaller than those between two independently determined structures in aqueous solution. The hydrogen bond system of the catalytic triad is intact in acetonitrile. The majority (99 of 119) of enzyme-bound, structural water molecules have such a great affinity to subtilisin that they are not displaced even in anhydrous acetonitrile. Of the 12 enzyme-bound acetonitrile molecules, 4 displace water molecules and 8 bind where no water had been observed before. One-third of all subtilisin-bound acetonitrile molecules reside in the active center, occupying the same region (P1, P2, and P3 binding sites) as the specific protein inhibitor eglin c.

-	~~{{STRUCTURE_1sca\| PDB=1sca \| SCENE= }}~~	+	Enzyme crystal structure in a neat organic solvent.,Fitzpatrick PA, Steinmetz AC, Ringe D, Klibanov AM Proc Natl Acad Sci U S A. 1993 Sep 15;90(18):8653-7. PMID:8378343<ref>PMID:8378343</ref>

-	~~===ENZYME CRYSTAL STRUCTURE IN A NEAT ORGANIC SOLVENT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_8378343}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1sca]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_licheniformis Bacillus licheniformis]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA].~~	+

	==See Also==		==See Also==
	*[[Subtilisin\|Subtilisin]]		*[[Subtilisin\|Subtilisin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:008378343<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bacillus licheniformis]]		[[Category: Bacillus licheniformis]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]

OCA at 06:58, 20 October 2012

OCA — Sat, 20 Oct 2012 06:58:16 GMT

←Older revision		Revision as of 06:58, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1sca]] is a 1 chain structure ~~of [[Subtilisin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA].	+	[[1sca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_licheniformis Bacillus licheniformis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SCA OCA].

	==See Also==		==See Also==

OCA at 14:53, 25 July 2012

OCA — Wed, 25 Jul 2012 14:53:14 GMT

←Older revision		Revision as of 14:53, 25 July 2012
Line 1:		Line 1:
	[[Image:1sca.png\|left\|200px]]		[[Image:1sca.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1sca", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1sca\| PDB=1sca \| SCENE= }}		{{STRUCTURE_1sca\| PDB=1sca \| SCENE= }}

	===ENZYME CRYSTAL STRUCTURE IN A NEAT ORGANIC SOLVENT===		===ENZYME CRYSTAL STRUCTURE IN A NEAT ORGANIC SOLVENT===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_8378343}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 8378343 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_8378343}}		{{ABSTRACT_PUBMED_8378343}}

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	==See Also==		==See Also==
-	*[[Subtilisin]]	+	*[[Subtilisin\|Subtilisin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~8378343~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008378343</ref><references group="xtra"/>
	[[Category: Bacillus licheniformis]]		[[Category: Bacillus licheniformis]]
	[[Category: Subtilisin]]		[[Category: Subtilisin]]