1snh - Revision history

OCA at 09:24, 6 December 2023

OCA — Wed, 06 Dec 2023 09:24:29 GMT

←Older revision		Revision as of 09:24, 6 December 2023
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	==Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer==		==Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer==
-	<StructureSection load='1snh' size='340' side='right'caption='[[1snh~~]], [[NMR_Ensembles_of_Models \| 12 NMR models~~]]' scene=''>	+	<StructureSection load='1snh' size='340' side='right'caption='[[1snh]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1snh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SNH FirstGlance]. <br>		<table><tr><td colspan='2'>[[1snh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SNH FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'>[[1pib\|1pib]]</div~~></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1snh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1snh OCA], [https://pdbe.org/1snh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1snh RCSB], [https://www.ebi.ac.uk/pdbsum/1snh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1snh ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1snh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1snh OCA], [https://pdbe.org/1snh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1snh RCSB], [https://www.ebi.ac.uk/pdbsum/1snh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1snh ProSAT]</span></td></tr>
	</table>		</table>
Line 21:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Akutsu, H]]	+	[[Category: Akutsu H]]
-	[[Category: Choi~~, B S~~]]	+	[[Category: Choi BS]]
-	[[Category: Ikegami, T]]	+	[[Category: Ikegami T]]
-	[[Category: Lee~~, J H~~]]	+	[[Category: Lee JH]]
-	[[Category: Park~~, C J~~]]	+	[[Category: Park CJ]]
-	[[Category: Shin~~, J S]]~~	+	[[Category: Shin JS]]
-	~~[[Category: Cpd]]~~	+
-	~~[[Category: Dna]]~~	+
-	~~[[Category: G-t mismatch]]~~	+
-	~~[[Category: Major groove widening~~]]	+

OCA at 07:34, 22 September 2021

OCA — Wed, 22 Sep 2021 07:34:41 GMT

←Older revision		Revision as of 07:34, 22 September 2021
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	<StructureSection load='1snh' size='340' side='right'caption='[[1snh]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>		<StructureSection load='1snh' size='340' side='right'caption='[[1snh]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1snh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1SNH FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1snh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SNH FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pib\|1pib]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1pib\|1pib]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1snh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1snh OCA], [~~http~~://pdbe.org/1snh PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1snh RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1snh PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1snh ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1snh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1snh OCA], [https://pdbe.org/1snh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1snh RCSB], [https://www.ebi.ac.uk/pdbsum/1snh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1snh ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 14:16, 18 December 2019

OCA — Wed, 18 Dec 2019 14:16:23 GMT

←Older revision		Revision as of 14:16, 18 December 2019
Line 1:		Line 1:

	==Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer==		==Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer==
-	<StructureSection load='1snh' size='340' side='right' caption='[[1snh]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>	+	<StructureSection load='1snh' size='340' side='right'caption='[[1snh]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1snh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SNH FirstGlance]. <br>		<table><tr><td colspan='2'>[[1snh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SNH FirstGlance]. <br>
Line 20:		Line 20:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Akutsu, H]]		[[Category: Akutsu, H]]
	[[Category: Choi, B S]]		[[Category: Choi, B S]]

OCA at 07:56, 14 March 2018

OCA — Wed, 14 Mar 2018 07:56:20 GMT

←Older revision		Revision as of 07:56, 14 March 2018
Line 1:		Line 1:
		+
	==Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer==		==Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer==
	<StructureSection load='1snh' size='340' side='right' caption='[[1snh]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>		<StructureSection load='1snh' size='340' side='right' caption='[[1snh]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1snh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SNH FirstGlance]. <br>		<table><tr><td colspan='2'>[[1snh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SNH FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pib\|1pib]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pib\|1pib]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1snh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1snh OCA], [http://pdbe.org/1snh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1snh RCSB], [http://www.ebi.ac.uk/pdbsum/1snh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1snh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1snh OCA], [http://pdbe.org/1snh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1snh RCSB], [http://www.ebi.ac.uk/pdbsum/1snh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1snh ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:14, 11 September 2015

OCA — Fri, 11 Sep 2015 04:14:12 GMT

←Older revision		Revision as of 04:14, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1snh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SNH FirstGlance]. <br>		<table><tr><td colspan='2'>[[1snh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SNH FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pib\|1pib]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pib\|1pib]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1snh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1snh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1snh RCSB], [http://www.ebi.ac.uk/pdbsum/1snh PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1snh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1snh OCA], [http://pdbe.org/1snh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1snh RCSB], [http://www.ebi.ac.uk/pdbsum/1snh PDBsum]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 14:		Line 14:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1snh" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 18:21, 15 January 2015

OCA — Thu, 15 Jan 2015 18:21:44 GMT

←Older revision		Revision as of 18:21, 15 January 2015
Line 18:		Line 18:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Akutsu, H.]]	+	[[Category: Akutsu, H]]
-	[[Category: Choi, B S.]]	+	[[Category: Choi, B S]]
-	[[Category: Ikegami, T.]]	+	[[Category: Ikegami, T]]
-	[[Category: Lee, J H.]]	+	[[Category: Lee, J H]]
-	[[Category: Park, C J.]]	+	[[Category: Park, C J]]
-	[[Category: Shin, J S.]]	+	[[Category: Shin, J S]]
	[[Category: Cpd]]		[[Category: Cpd]]
	[[Category: Dna]]		[[Category: Dna]]
	[[Category: G-t mismatch]]		[[Category: G-t mismatch]]
	[[Category: Major groove widening]]		[[Category: Major groove widening]]

OCA at 08:24, 8 October 2014

OCA — Wed, 08 Oct 2014 08:24:35 GMT

←Older revision		Revision as of 08:24, 8 October 2014
Line 1:		Line 1:
-	[[~~Image:~~1snh.~~png~~\|~~left~~\|~~200px~~]]	+	==Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer==
		+	<StructureSection load='1snh' size='340' side='right' caption='[[1snh]], [[NMR_Ensembles_of_Models \| 12 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1snh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SNH FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1pib\|1pib]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1snh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1snh OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1snh RCSB], [http://www.ebi.ac.uk/pdbsum/1snh PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cis-syn cyclobutane pyrimidine dimer (CPD) is a cytotoxic, mutagenic and carcinogenic DNA photoproduct and is repaired by the nucleotide excision repair (NER) pathway in mammalian cells. The XPC-hHR23B complex as the initiator of global genomic NER binds to sites of certain kinds of DNA damage. Although CPDs are rarely recognized by the XPC-hHR23B complex, the presence of mismatched bases opposite a CPD significantly increased the binding affinity of the XPC-hHR23B complex to the CPD. In order to decipher the properties of the DNA structures that determine the binding affinity for XPC-hHR23B to DNA, we carried out structural analyses of the various types of CPDs by NMR spectroscopy. The DNA duplex which contains a single 3' TG wobble pair in a CPD (CPD/GA duplex) induces little conformational distortion. However, severe distortion of the helical conformation occurs when a CPD contains double TG wobble pairs (CPD/GG duplex) even though the T residues of the CPD form stable hydrogen bonds with the opposite G residues. The helical bending angle of the CPD/GG duplex was larger than those of the CPD/GA duplex and properly matched CPD/AA duplex. The fluctuation of the backbone conformation and significant changes in the widths of the major and minor grooves at the double T*G wobble paired site were also observed in the CPD/GG duplex. These structural features were also found in a duplex that contains the (6-4) adduct, which is efficiently recognized by the XPC-hHR23B complex. Thus, we suggest that the unique structural features of the DNA double helix (that is, helical bending, flexible backbone conformation, and significant changes of the major and/or minor grooves) might be important factors in determining the binding affinity of the XPC-hHR23B complex to DNA.

-	~~{{STRUCTURE_1snh\| PDB=1snh \| SCENE= }}~~	+	NMR structure of the DNA decamer duplex containing double T*G mismatches of cis-syn cyclobutane pyrimidine dimer: implications for DNA damage recognition by the XPC-hHR23B complex.,Lee JH, Park CJ, Shin JS, Ikegami T, Akutsu H, Choi BS Nucleic Acids Res. 2004 Apr 30;32(8):2474-81. Print 2004. PMID:15121904<ref>PMID:15121904</ref>

-	~~===Solution structure~~ of the ~~DNA Decamer Duplex Containing Double TG Mismatches~~ of ~~Cis-syn Cyclobutane Pyrimidine Dimer===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15121904}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1snh]] is a 2 chain structure. Full experimental information is available from [http:~~//~~oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA].~~	+	</StructureSection>
	[[Category: Akutsu, H.]]		[[Category: Akutsu, H.]]
	[[Category: Choi, B S.]]		[[Category: Choi, B S.]]

OCA: Protected "1snh" [edit=sysop:move=sysop]

OCA — Fri, 04 Jan 2013 10:40:23 GMT

Protected "1snh" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:40, 4 January 2013

OCA at 10:40, 4 January 2013

OCA — Fri, 04 Jan 2013 10:40:20 GMT

←Older revision		Revision as of 10:40, 4 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1snh.png\|left\|200px]]		[[Image:1snh.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1snh", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1snh\| PDB=1snh \| SCENE= }}		{{STRUCTURE_1snh\| PDB=1snh \| SCENE= }}

	===Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer===		===Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15121904}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15121904 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15121904}}		{{ABSTRACT_PUBMED_15121904}}

	==About this Structure==		==About this Structure==
-	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA].	+	[[1snh]] is a 2 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA].
-		+
-	~~==Reference==~~	+
-	NMR structure of the DNA decamer duplex containing double T*G mismatches of cis-syn cyclobutane pyrimidine dimer: implications for DNA damage recognition by the XPC-hHR23B complex., Lee JH, Park CJ, Shin JS, Ikegami T, Akutsu H, Choi BS, Nucleic Acids Res. 2004 Apr 30;32(8):2474-81. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15121904 15121904]	+
	[[Category: Akutsu, H.]]		[[Category: Akutsu, H.]]
	[[Category: Choi, B S.]]		[[Category: Choi, B S.]]
Line 34:		Line 19:
	[[Category: G-t mismatch]]		[[Category: G-t mismatch]]
	[[Category: Major groove widening]]		[[Category: Major groove widening]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:54:57 2008''

OCA at 16:55, 28 July 2008

OCA — Mon, 28 Jul 2008 16:55:08 GMT

←Older revision		Revision as of 16:55, 28 July 2008
Line 1:		Line 1:
-	[[Image:1snh.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1snh.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1snh\| PDB=1snh \| SCENE= }}		{{STRUCTURE_1snh\| PDB=1snh \| SCENE= }}

-	~~'''~~Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer~~'''~~	+	===Solution structure of the DNA Decamer Duplex Containing Double TG Mismatches of Cis-syn Cyclobutane Pyrimidine Dimer===


-	~~==Overview==~~	+	<!--
-	The ~~cis-syn cyclobutane pyrimidine dimer (CPD) is a cytotoxic~~, mutagenic and carcinogenic DNA photoproduct and is repaired by the nucleotide excision repair (NER) pathway in mammalian cells. The XPC-hHR23B complex as the initiator of global genomic NER binds to sites of certain kinds of DNA damage. Although CPDs are rarely recognized by the XPC-hHR23B complex, the ~~presence of mismatched bases opposite a CPD significantly increased the binding affinity of the XPC-hHR23B complex~~ to the CPD. In order to decipher the properties of the DNA structures that determine the binding affinity for XPC-hHR23B to DNA, we carried out structural analyses of the various types of CPDs by NMR spectroscopy. The DNA duplex which contains a single 3' TG wobble pair in a CPD (~~CPD~~/~~GA duplex) induces little conformational distortion. However, severe distortion of the helical conformation occurs when a CPD contains double TG wobble pairs (CPD~~/~~GG duplex) even though the T residues of the CPD form stable hydrogen bonds with the opposite G residues~~. ~~The helical bending angle of the CPD/GG duplex was larger than those of the CPD/GA duplex and properly matched CPD/AA duplex~~. The fluctuation of the backbone conformation and significant changes in the widths of the major and minor grooves at the double T*G wobble paired site were also observed in the CPD/GG duplex. These structural features were also found in a duplex that contains the (6-4) ~~adduct~~, ~~which~~ is ~~efficiently recognized by~~ the ~~XPC-hHR23B complex~~. Thus, we suggest that the unique structural features of the DNA double helix (that is, helical bending, flexible backbone conformation, and significant changes of the major and/or minor grooves) might be important factors in determining the binding affinity of the XPC-~~hHR23B complex to DNA.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_15121904}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15121904 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15121904}}

	==About this Structure==		==About this Structure==
-	Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA].	+	Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SNH OCA].

	==Reference==		==Reference==
Line 30:		Line 34:
	[[Category: G-t mismatch]]		[[Category: G-t mismatch]]
	[[Category: Major groove widening]]		[[Category: Major groove widening]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 08~~:55:19 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:54:57 2008''