1sqf - Revision history

OCA at 08:32, 14 February 2024

OCA — Wed, 14 Feb 2024 08:32:58 GMT

←Older revision		Revision as of 08:32, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1sqf' size='340' side='right'caption='[[1sqf]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1sqf' size='340' side='right'caption='[[1sqf]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sqf]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1SQF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sqf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SQF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SAM:S~~-~~ADENOSYLMETHIONINE'>SAM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>SUN, FMU, FMV, RSMB, B3289 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [~~http~~://pdbe.org/1sqf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1sqf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1sqf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [https://pdbe.org/1sqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [https://www.ebi.ac.uk/pdbsum/1sqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sqf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/RSMB_ECOLI RSMB_ECOLI]] Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.<ref>PMID:10194318</ref>	+	[https://www.uniprot.org/uniprot/RSMB_ECOLI RSMB_ECOLI] Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.<ref>PMID:10194318</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sqf ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sqf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structure of E. coli Fmu, determined at 1.65 A resolution for the apoenzyme and 2.1 A resolution in complex with AdoMet, is the first representative of the 5-methylcytosine RNA methyltransferase family that includes the human nucleolar proliferation-associated protein p120. Fmu contains three subdomains which share structural homology to DNA m(5)C methyltransferases and two RNA binding protein families. In the binary complex, the AdoMet cofactor is positioned within the active site near a novel arrangement of two conserved cysteines that function in cytosine methylation. The site is surrounded by a positively charged cleft large enough to bind its unique target stem loop within 16S rRNA. Docking of this stem loop RNA into the structure followed by molecular mechanics shows that the Fmu structure is consistent with binding to the folded RNA substrate.
-
-	The first structure of an RNA m5C methyltransferase, Fmu, provides insight into catalytic mechanism and specific binding of RNA substrate.,Foster PG, Nunes CR, Greene P, Moustakas D, Stroud RM Structure. 2003 Dec;11(12):1609-20. PMID:14656444<ref>PMID:14656444</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1sqf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Foster~~, P G~~]]	+	[[Category: Foster PG]]
-	[[Category: Greene, P]]	+	[[Category: Greene P]]
-	[[Category: Moustakas, D]]	+	[[Category: Moustakas D]]
-	[[Category: Nunes~~, C R~~]]	+	[[Category: Nunes CR]]
-	[[Category: Stroud~~, R M]]~~	+	[[Category: Stroud RM]]
-	~~[[Category: Methyltransferase-fold]]~~	+
-	~~[[Category: Mixed beta sheet]]~~	+
-	~~[[Category: Rna-binding domain]]~~	+
-	~~[[Category: Rossmann-fold]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 11:32, 24 December 2020

OCA — Thu, 24 Dec 2020 11:32:16 GMT

←Older revision		Revision as of 11:32, 24 December 2020
Line 1:		Line 1:

	==The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution==		==The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution==
-	<StructureSection load='1sqf' size='340' side='right' caption='[[1sqf]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1sqf' size='340' side='right'caption='[[1sqf]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1SQF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1SQF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SUN, FMU, FMV, RSMB, B3289 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SUN, FMU, FMV, RSMB, B3289 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [http://pdbe.org/1sqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [http://www.ebi.ac.uk/pdbsum/1sqf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sqf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [http://pdbe.org/1sqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [http://www.ebi.ac.uk/pdbsum/1sqf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sqf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Foster, P G]]		[[Category: Foster, P G]]
	[[Category: Greene, P]]		[[Category: Greene, P]]

OCA at 07:59, 14 March 2018

OCA — Wed, 14 Mar 2018 07:59:26 GMT

←Older revision		Revision as of 07:59, 14 March 2018
Line 1:		Line 1:
		+
	==The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution==		==The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution==
	<StructureSection load='1sqf' size='340' side='right' caption='[[1sqf]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1sqf' size='340' side='right' caption='[[1sqf]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SUN, FMU, FMV, RSMB, B3289 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SUN, FMU, FMV, RSMB, B3289 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [http://pdbe.org/1sqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [http://www.ebi.ac.uk/pdbsum/1sqf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [http://pdbe.org/1sqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [http://www.ebi.ac.uk/pdbsum/1sqf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sqf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sq/1sqf_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sq/1sqf_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sqf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 22:34, 11 September 2015

OCA — Fri, 11 Sep 2015 22:34:25 GMT

←Older revision		Revision as of 22:34, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1sqf' size='340' side='right' caption='[[1sqf]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1sqf' size='340' side='right' caption='[[1sqf]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SQF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SQF FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SUN, FMU, FMV, RSMB, B3289 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SUN, FMU, FMV, RSMB, B3289 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [http://www.ebi.ac.uk/pdbsum/1sqf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [http://pdbe.org/1sqf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [http://www.ebi.ac.uk/pdbsum/1sqf PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1sqf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Foster, P G]]		[[Category: Foster, P G]]
	[[Category: Greene, P]]		[[Category: Greene, P]]

OCA at 20:20, 25 December 2014

OCA — Thu, 25 Dec 2014 20:20:39 GMT

←Older revision		Revision as of 20:20, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1sqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SQF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1sqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SQF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SUN, FMU, FMV, RSMB, B3289 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SUN, FMU, FMV, RSMB, B3289 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [http://www.ebi.ac.uk/pdbsum/1sqf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [http://www.ebi.ac.uk/pdbsum/1sqf PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/RSMB_ECOLI RSMB_ECOLI]] Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA.<ref>PMID:10194318</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Foster, P G.]]	+	[[Category: Foster, P G]]
-	[[Category: Greene, P.]]	+	[[Category: Greene, P]]
-	[[Category: Moustakas, D.]]	+	[[Category: Moustakas, D]]
-	[[Category: Nunes, C R.]]	+	[[Category: Nunes, C R]]
-	[[Category: Stroud, R M.]]	+	[[Category: Stroud, R M]]
	[[Category: Methyltransferase-fold]]		[[Category: Methyltransferase-fold]]
	[[Category: Mixed beta sheet]]		[[Category: Mixed beta sheet]]

OCA at 15:15, 29 September 2014

OCA — Mon, 29 Sep 2014 15:15:22 GMT

←Older revision		Revision as of 15:15, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1sqf.~~png~~\|~~left~~\|200px]]	+	==The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution==
		+	<StructureSection load='1sqf' size='340' side='right' caption='[[1sqf]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1sqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SQF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SUN, FMU, FMV, RSMB, B3289 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sqf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sqf RCSB], [http://www.ebi.ac.uk/pdbsum/1sqf PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sq/1sqf_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of E. coli Fmu, determined at 1.65 A resolution for the apoenzyme and 2.1 A resolution in complex with AdoMet, is the first representative of the 5-methylcytosine RNA methyltransferase family that includes the human nucleolar proliferation-associated protein p120. Fmu contains three subdomains which share structural homology to DNA m(5)C methyltransferases and two RNA binding protein families. In the binary complex, the AdoMet cofactor is positioned within the active site near a novel arrangement of two conserved cysteines that function in cytosine methylation. The site is surrounded by a positively charged cleft large enough to bind its unique target stem loop within 16S rRNA. Docking of this stem loop RNA into the structure followed by molecular mechanics shows that the Fmu structure is consistent with binding to the folded RNA substrate.

-	~~{{STRUCTURE_1sqf\| PDB=1sqf \| SCENE= }}~~	+	The first structure of an RNA m5C methyltransferase, Fmu, provides insight into catalytic mechanism and specific binding of RNA substrate.,Foster PG, Nunes CR, Greene P, Moustakas D, Stroud RM Structure. 2003 Dec;11(12):1609-20. PMID:14656444<ref>PMID:14656444</ref>

-	~~===The crystal structure~~ of E. ~~coli Fmu binary complex with~~ S~~-Adenosylmethionine at 2~~.~~1 A resolution===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14656444}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1sqf]] is a 1 chain structure with sequence from [http:~~/~~/en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:014656444<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Foster, P G.]]		[[Category: Foster, P G.]]

OCA: Protected "1sqf" [edit=sysop:move=sysop]

OCA — Fri, 04 Jan 2013 10:45:08 GMT

Protected "1sqf" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:45, 4 January 2013

OCA at 10:45, 4 January 2013

OCA — Fri, 04 Jan 2013 10:45:04 GMT

←Older revision		Revision as of 10:45, 4 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1sqf.png\|left\|200px]]		[[Image:1sqf.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1sqf", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1sqf\| PDB=1sqf \| SCENE= }}		{{STRUCTURE_1sqf\| PDB=1sqf \| SCENE= }}

	===The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution===		===The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_14656444}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 14656444 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_14656444}}		{{ABSTRACT_PUBMED_14656444}}

	==About this Structure==		==About this Structure==
-	~~1SQF~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA].	+	[[1sqf]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~14656444~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:014656444</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Foster, P G.]]		[[Category: Foster, P G.]]
Line 34:		Line 22:
	[[Category: Rna-binding domain]]		[[Category: Rna-binding domain]]
	[[Category: Rossmann-fold]]		[[Category: Rossmann-fold]]
-		+	[[Category: Transferase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 06:01:24 2009''~~	+

OCA at 04:01, 18 February 2009

OCA — Wed, 18 Feb 2009 04:01:25 GMT

←Older revision		Revision as of 04:01, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1SQF is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA].	+	1SQF is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SQF OCA].

	==Reference==		==Reference==
-	The first structure of an RNA m5C methyltransferase, Fmu, provides insight into catalytic mechanism and specific binding of RNA substrate., Foster PG, Nunes CR, Greene P, Moustakas D, Stroud RM, Structure. 2003 Dec;11(12):1609-20. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/14656444 14656444]~~	+	<ref group="xtra">PMID:14656444</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Foster, P G.]]		[[Category: Foster, P G.]]
	[[Category: Greene, P.]]		[[Category: Greene, P.]]
Line 36:		Line 35:
	[[Category: Rossmann-fold]]		[[Category: Rossmann-fold]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 12~~:45:~~03 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 06:01:24 2009''

OCA at 09:45, 29 July 2008

OCA — Tue, 29 Jul 2008 09:45:05 GMT

←Older revision		Revision as of 09:45, 29 July 2008
Line 1:		Line 1:
-	[[Image:1sqf.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1sqf.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1sqf\| PDB=1sqf \| SCENE= }}		{{STRUCTURE_1sqf\| PDB=1sqf \| SCENE= }}

-	~~'''~~The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution~~'''~~	+	===The crystal structure of E. coli Fmu binary complex with S-Adenosylmethionine at 2.1 A resolution===


-	~~==Overview==~~	+	<!--
-	The ~~crystal structure of E. coli Fmu~~, ~~determined at 1.65 A resolution for the apoenzyme and 2.1 A resolution in complex with AdoMet~~, is the ~~first representative of~~ the ~~5-methylcytosine RNA methyltransferase family that includes the human nucleolar proliferation-associated protein p120~~. ~~Fmu contains three subdomains which share structural homology to DNA m(5~~)~~C methyltransferases and two RNA binding protein families. In the binary complex~~, ~~the AdoMet cofactor~~ is ~~positioned within~~ the active site near a novel arrangement of two conserved cysteines that function in cytosine methylation. The site is surrounded by a positively charged cleft large enough to bind its unique target stem loop within 16S rRNA. Docking of this stem loop RNA into the structure followed by molecular mechanics shows that the Fmu structure is consistent with binding to the folded RNA substrate.	+	The line below this paragraph, {{ABSTRACT_PUBMED_14656444}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 14656444 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_14656444}}

	==About this Structure==		==About this Structure==
Line 31:		Line 35:
	[[Category: Rna-binding domain]]		[[Category: Rna-binding domain]]
	[[Category: Rossmann-fold]]		[[Category: Rossmann-fold]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 09~~:01:05 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:45:03 2008''