1sr4 - Revision history

OCA at 08:34, 1 May 2024

OCA — Wed, 01 May 2024 08:34:31 GMT

←Older revision		Revision as of 08:34, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='1sr4' size='340' side='right'caption='[[1sr4]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1sr4' size='340' side='right'caption='[[1sr4]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sr4]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_ulceris_cancrosi"_kruse_1896 "bacillus ulceris cancrosi" kruse 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1SR4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sr4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SR4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>CDTA, HD0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~730~~ "~~Bacillus ulceris cancrosi~~" ~~Kruse 1896])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [~~http~~://pdbe.org/1sr4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1sr4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1sr4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [https://pdbe.org/1sr4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [https://www.ebi.ac.uk/pdbsum/1sr4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sr4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CDTA_HAEDU CDTA_HAEDU]] CDTs are cytotoxins which induce host cell distension, growth arrest in G2/M phase, nucleus swelling, and chromatin fragmentation in HeLa cells. CdtA, along with CdtC, probably forms a heterodimeric subunit required for the delivery of CdtB.	+	[https://www.uniprot.org/uniprot/CDTA_HAEDU CDTA_HAEDU] CDTs are cytotoxins which induce host cell distension, growth arrest in G2/M phase, nucleus swelling, and chromatin fragmentation in HeLa cells. CdtA, along with CdtC, probably forms a heterodimeric subunit required for the delivery of CdtB.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 20:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sr4 ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sr4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The tripartite cytolethal distending toxin (CDT) induces cell cycle arrest and apoptosis in eukaryotic cells. The subunits CdtA and CdtC associate with the nuclease CdtB to form a holotoxin that translocates CdtB into the host cell, where it acts as a genotoxin by creating DNA lesions. Here we show that the crystal structure of the holotoxin from Haemophilus ducreyi reveals that CDT consists of an enzyme of the DNase-I family, bound to two ricin-like lectin domains. CdtA, CdtB and CdtC form a ternary complex with three interdependent molecular interfaces, characterized by globular, as well as extensive non-globular, interactions. The lectin subunits form a deeply grooved, highly aromatic surface that we show to be critical for toxicity. The holotoxin possesses a steric block of the CdtB active site by means of a non-globular extension of the CdtC subunit, and we identify putative DNA binding residues in CdtB that are essential for toxin activity.
-
-	Assembly and function of a bacterial genotoxin.,Nesic D, Hsu Y, Stebbins CE Nature. 2004 May 27;429(6990):429-33. PMID:15164065<ref>PMID:15164065</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1sr4" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ulceris cancrosi kruse 1896]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hsu, Y]]	+	[[Category: Hsu Y]]
-	[[Category: Nesic, D]]	+	[[Category: Nesic D]]
-	[[Category: Stebbins~~, C E]]~~	+	[[Category: Stebbins CE]]
-	~~[[Category: Apoptosis]]~~	+
-	~~[[Category: Bacterial]]~~	+
-	~~[[Category: Cell cycle]]~~	+
-	~~[[Category: Cytotoxin]]~~	+
-	~~[[Category: Deoxyribonuclease i]]~~	+
-	~~[[Category: Dna damage]]~~	+
-	~~[[Category: Genotoxin]]~~	+
-	~~[[Category: Haemophilus ducreyi]]~~	+
-	~~[[Category: Lectin]]~~	+
-	~~[[Category: Toxin]]~~	+
-	~~[[Category: Virulence~~]]	+

OCA at 11:32, 24 December 2020

OCA — Thu, 24 Dec 2020 11:32:45 GMT

←Older revision		Revision as of 11:32, 24 December 2020
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	==Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin==		==Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin==
-	<StructureSection load='1sr4' size='340' side='right' caption='[[1sr4]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1sr4' size='340' side='right'caption='[[1sr4]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sr4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_ulceris_cancrosi"_kruse_1896 "bacillus ulceris cancrosi" kruse 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1SR4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sr4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_ulceris_cancrosi"_kruse_1896 "bacillus ulceris cancrosi" kruse 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1SR4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDTA, HD0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=730 "Bacillus ulceris cancrosi" Kruse 1896])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDTA, HD0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=730 "Bacillus ulceris cancrosi" Kruse 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [http://pdbe.org/1sr4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [http://www.ebi.ac.uk/pdbsum/1sr4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sr4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [http://pdbe.org/1sr4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [http://www.ebi.ac.uk/pdbsum/1sr4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sr4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus ulceris cancrosi kruse 1896]]		[[Category: Bacillus ulceris cancrosi kruse 1896]]
		+	[[Category: Large Structures]]
	[[Category: Hsu, Y]]		[[Category: Hsu, Y]]
	[[Category: Nesic, D]]		[[Category: Nesic, D]]

OCA at 07:55, 14 March 2018

OCA — Wed, 14 Mar 2018 07:55:07 GMT

←Older revision		Revision as of 07:55, 14 March 2018
Line 1:		Line 1:
		+
	==Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin==		==Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin==
	<StructureSection load='1sr4' size='340' side='right' caption='[[1sr4]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1sr4' size='340' side='right' caption='[[1sr4]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDTA, HD0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=730 "Bacillus ulceris cancrosi" Kruse 1896])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDTA, HD0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=730 "Bacillus ulceris cancrosi" Kruse 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [http://pdbe.org/1sr4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [http://www.ebi.ac.uk/pdbsum/1sr4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [http://pdbe.org/1sr4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [http://www.ebi.ac.uk/pdbsum/1sr4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sr4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sr/1sr4_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sr/1sr4_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 13:36, 7 February 2016

OCA — Sun, 07 Feb 2016 13:36:08 GMT

←Older revision		Revision as of 13:36, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sr4 ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 01:58, 10 September 2015

OCA — Thu, 10 Sep 2015 01:58:36 GMT

←Older revision		Revision as of 01:58, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1sr4' size='340' side='right' caption='[[1sr4]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1sr4' size='340' side='right' caption='[[1sr4]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sr4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Haemophilus_ducreyi Haemophilus ducreyi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SR4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sr4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_ulceris_cancrosi"_kruse_1896 "bacillus ulceris cancrosi" kruse 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SR4 FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDTA, HD0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=730 ~~Haemophilus ducreyi~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDTA, HD0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=730 "Bacillus ulceris cancrosi" Kruse 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [http://www.ebi.ac.uk/pdbsum/1sr4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [http://pdbe.org/1sr4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [http://www.ebi.ac.uk/pdbsum/1sr4 PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1sr4" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Haemophilus ducreyi~~]]	+	[[Category: Bacillus ulceris cancrosi kruse 1896]]
	[[Category: Hsu, Y]]		[[Category: Hsu, Y]]
	[[Category: Nesic, D]]		[[Category: Nesic, D]]
Line 42:		Line 43:
	[[Category: Dna damage]]		[[Category: Dna damage]]
	[[Category: Genotoxin]]		[[Category: Genotoxin]]
		+	[[Category: Haemophilus ducreyi]]
	[[Category: Lectin]]		[[Category: Lectin]]
	[[Category: Toxin]]		[[Category: Toxin]]
	[[Category: Virulence]]		[[Category: Virulence]]

OCA at 15:27, 24 December 2014

OCA — Wed, 24 Dec 2014 15:27:37 GMT

←Older revision		Revision as of 15:27, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1sr4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SR4 FirstGlance]. <br>		<table><tr><td colspan='2'>[[1sr4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SR4 FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDTA, HD0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=730 Haemophilus ducreyi])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDTA, HD0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=730 Haemophilus ducreyi])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [http://www.ebi.ac.uk/pdbsum/1sr4 PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [http://www.ebi.ac.uk/pdbsum/1sr4 PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CDTA_HAEDU CDTA_HAEDU]] CDTs are cytotoxins which induce host cell distension, growth arrest in G2/M phase, nucleus swelling, and chromatin fragmentation in HeLa cells. CdtA, along with CdtC, probably forms a heterodimeric subunit required for the delivery of CdtB.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Haemophilus ducreyi]]		[[Category: Haemophilus ducreyi]]
-	[[Category: Hsu, Y.]]	+	[[Category: Hsu, Y]]
-	[[Category: Nesic, D.]]	+	[[Category: Nesic, D]]
-	[[Category: Stebbins, C E.]]	+	[[Category: Stebbins, C E]]
	[[Category: Apoptosis]]		[[Category: Apoptosis]]
	[[Category: Bacterial]]		[[Category: Bacterial]]
Line 40:		Line 42:
	[[Category: Dna damage]]		[[Category: Dna damage]]
	[[Category: Genotoxin]]		[[Category: Genotoxin]]
-	[[Category: Haemophilus ducreyi]]
	[[Category: Lectin]]		[[Category: Lectin]]
	[[Category: Toxin]]		[[Category: Toxin]]
	[[Category: Virulence]]		[[Category: Virulence]]

OCA at 16:36, 29 September 2014

OCA — Mon, 29 Sep 2014 16:36:36 GMT

←Older revision		Revision as of 16:36, 29 September 2014
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-	[[~~Image:~~1sr4.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin==
		+	<StructureSection load='1sr4' size='340' side='right' caption='[[1sr4]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1sr4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SR4 FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CDTA, HD0902 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=730 Haemophilus ducreyi])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sr4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sr4 OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sr4 RCSB], [http://www.ebi.ac.uk/pdbsum/1sr4 PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sr/1sr4_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The tripartite cytolethal distending toxin (CDT) induces cell cycle arrest and apoptosis in eukaryotic cells. The subunits CdtA and CdtC associate with the nuclease CdtB to form a holotoxin that translocates CdtB into the host cell, where it acts as a genotoxin by creating DNA lesions. Here we show that the crystal structure of the holotoxin from Haemophilus ducreyi reveals that CDT consists of an enzyme of the DNase-I family, bound to two ricin-like lectin domains. CdtA, CdtB and CdtC form a ternary complex with three interdependent molecular interfaces, characterized by globular, as well as extensive non-globular, interactions. The lectin subunits form a deeply grooved, highly aromatic surface that we show to be critical for toxicity. The holotoxin possesses a steric block of the CdtB active site by means of a non-globular extension of the CdtC subunit, and we identify putative DNA binding residues in CdtB that are essential for toxin activity.

-	~~{{STRUCTURE_1sr4\| PDB=1sr4 \| SCENE= }}~~	+	Assembly and function of a bacterial genotoxin.,Nesic D, Hsu Y, Stebbins CE Nature. 2004 May 27;429(6990):429-33. PMID:15164065<ref>PMID:15164065</ref>

-	~~===Crystal Structure~~ of the ~~Haemophilus ducreyi cytolethal distending toxin===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15164065}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1sr4]] is a 3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015164065<~~/~~ref~~><~~ref group="xtra">PMID:017009862</ref><references group="xtra"~~/>	+
	[[Category: Haemophilus ducreyi]]		[[Category: Haemophilus ducreyi]]
	[[Category: Hsu, Y.]]		[[Category: Hsu, Y.]]

OCA: Protected "1sr4" [edit=sysop:move=sysop]

OCA — Fri, 04 Jan 2013 10:38:34 GMT

Protected "1sr4" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:38, 4 January 2013

OCA at 10:38, 4 January 2013

OCA — Fri, 04 Jan 2013 10:38:31 GMT

←Older revision		Revision as of 10:38, 4 January 2013
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-	{{Seed}}
	[[Image:1sr4.png\|left\|200px]]		[[Image:1sr4.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1sr4", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1sr4\| PDB=1sr4 \| SCENE= }}		{{STRUCTURE_1sr4\| PDB=1sr4 \| SCENE= }}

	===Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin===		===Crystal Structure of the Haemophilus ducreyi cytolethal distending toxin===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15164065}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15164065 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_15164065}}		{{ABSTRACT_PUBMED_15164065}}

	==About this Structure==		==About this Structure==
-	~~1SR4~~ is a 3 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA].	+	[[1sr4]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15164065~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015164065</ref><ref group="xtra">PMID:017009862</ref><references group="xtra"/>
	[[Category: Haemophilus ducreyi]]		[[Category: Haemophilus ducreyi]]
	[[Category: Hsu, Y.]]		[[Category: Hsu, Y.]]
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	[[Category: Toxin]]		[[Category: Toxin]]
	[[Category: Virulence]]		[[Category: Virulence]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:09:34 2009''

OCA at 12:09, 16 February 2009

OCA — Mon, 16 Feb 2009 12:09:35 GMT

←Older revision		Revision as of 12:09, 16 February 2009
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	==About this Structure==		==About this Structure==
-	1SR4 is a ~~[[Protein complex]]~~ structure of sequences from [http://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA].	+	1SR4 is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Haemophilus_ducreyi Haemophilus ducreyi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SR4 OCA].

	==Reference==		==Reference==
-	~~Assembly and function of a bacterial genotoxin., Nesic D, Hsu Y, Stebbins CE, Nature. 2004 May 27;429(6990):429-33.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15164065 15164065]~~	+	<ref group="xtra">PMID:15164065</ref><references group="xtra"/>
	[[Category: Haemophilus ducreyi]]		[[Category: Haemophilus ducreyi]]
-	[[Category: Protein complex]]
	[[Category: Hsu, Y.]]		[[Category: Hsu, Y.]]
	[[Category: Nesic, D.]]		[[Category: Nesic, D.]]
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	[[Category: Virulence]]		[[Category: Virulence]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Jul 27~~ 16:43:~~54 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:09:34 2009''