1swu - Revision history

OCA at 06:21, 23 August 2023

OCA — Wed, 23 Aug 2023 06:21:20 GMT

←Older revision		Revision as of 06:21, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1swu' size='340' side='right'caption='[[1swu]], [[Resolution\|resolution]] 1.14Å' scene=''>		<StructureSection load='1swu' size='340' side='right'caption='[[1swu]], [[Resolution\|resolution]] 1.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~As_4.1583 As 4.1583~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SWU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SWU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.14Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swu OCA], [https://pdbe.org/1swu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1swu RCSB], [https://www.ebi.ac.uk/pdbsum/1swu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1swu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swu OCA], [https://pdbe.org/1swu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1swu RCSB], [https://www.ebi.ac.uk/pdbsum/1swu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1swu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Freitag, S~~]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: ~~Klumb, L A]]~~	+	[[Category: Freitag S]]
-	~~[[Category: Stayton, P~~ S]]	+	[[Category: Klumb LA]]
-	[[Category: ~~Stenkamp, R E~~]]	+	[[Category: Le Trong I]]
-	[[Category: Trong, I Le]]	+	[[Category: Stayton PS]]
-	[[Category: ~~Biotin binding protein~~]]	+	[[Category: Stenkamp RE]]
-	[[Category: ~~Protein binding~~]]	+

OCA at 07:38, 22 September 2021

OCA — Wed, 22 Sep 2021 07:38:28 GMT

←Older revision		Revision as of 07:38, 22 September 2021
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	<StructureSection load='1swu' size='340' side='right'caption='[[1swu]], [[Resolution\|resolution]] 1.14Å' scene=''>		<StructureSection load='1swu' size='340' side='right'caption='[[1swu]], [[Resolution\|resolution]] 1.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1SWU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SWU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swu OCA], [~~http~~://pdbe.org/1swu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1swu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1swu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1swu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swu OCA], [https://pdbe.org/1swu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1swu RCSB], [https://www.ebi.ac.uk/pdbsum/1swu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1swu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 14:21, 18 December 2019

OCA — Wed, 18 Dec 2019 14:21:34 GMT

←Older revision		Revision as of 14:21, 18 December 2019
Line 1:		Line 1:

	==STREPTAVIDIN MUTANT Y43F==		==STREPTAVIDIN MUTANT Y43F==
-	<StructureSection load='1swu' size='340' side='right' caption='[[1swu]], [[Resolution\|resolution]] 1.14Å' scene=''>	+	<StructureSection load='1swu' size='340' side='right'caption='[[1swu]], [[Resolution\|resolution]] 1.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SWU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SWU FirstGlance]. <br>
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Avidin\|Avidin]]	+	*[[Avidin 3D structures\|Avidin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: As 4 1583]]		[[Category: As 4 1583]]
		+	[[Category: Large Structures]]
	[[Category: Freitag, S]]		[[Category: Freitag, S]]
	[[Category: Klumb, L A]]		[[Category: Klumb, L A]]

OCA at 08:01, 14 March 2018

OCA — Wed, 14 Mar 2018 08:01:14 GMT

←Older revision		Revision as of 08:01, 14 March 2018
Line 1:		Line 1:
		+
	==STREPTAVIDIN MUTANT Y43F==		==STREPTAVIDIN MUTANT Y43F==
	<StructureSection load='1swu' size='340' side='right' caption='[[1swu]], [[Resolution\|resolution]] 1.14Å' scene=''>		<StructureSection load='1swu' size='340' side='right' caption='[[1swu]], [[Resolution\|resolution]] 1.14Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SWU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SWU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swu OCA], [http://pdbe.org/1swu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1swu RCSB], [http://www.ebi.ac.uk/pdbsum/1swu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swu OCA], [http://pdbe.org/1swu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1swu RCSB], [http://www.ebi.ac.uk/pdbsum/1swu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1swu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sw/1swu_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sw/1swu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1swu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:35, 11 September 2015

OCA — Fri, 11 Sep 2015 15:35:56 GMT

←Older revision		Revision as of 15:35, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1swu' size='340' side='right' caption='[[1swu]], [[Resolution\|resolution]] 1.14Å' scene=''>		<StructureSection load='1swu' size='340' side='right' caption='[[1swu]], [[Resolution\|resolution]] 1.14Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_avidinii Streptomyces avidinii~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SWU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SWU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1swu RCSB], [http://www.ebi.ac.uk/pdbsum/1swu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swu OCA], [http://pdbe.org/1swu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1swu RCSB], [http://www.ebi.ac.uk/pdbsum/1swu PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1swu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces avidinii~~]]	+	[[Category: As 4 1583]]
	[[Category: Freitag, S]]		[[Category: Freitag, S]]
	[[Category: Klumb, L A]]		[[Category: Klumb, L A]]

OCA at 18:28, 25 December 2014

OCA — Thu, 25 Dec 2014 18:28:26 GMT

←Older revision		Revision as of 18:28, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SWU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SWU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1swu RCSB], [http://www.ebi.ac.uk/pdbsum/1swu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1swu RCSB], [http://www.ebi.ac.uk/pdbsum/1swu PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
-	[[Category: Freitag, S.]]	+	[[Category: Freitag, S]]
-	[[Category: Klumb, L A.]]	+	[[Category: Klumb, L A]]
-	[[Category: Stayton, P S.]]	+	[[Category: Stayton, P S]]
-	[[Category: Stenkamp, R E.]]	+	[[Category: Stenkamp, R E]]
-	[[Category: Trong, I Le.]]	+	[[Category: Trong, I Le]]
	[[Category: Biotin binding protein]]		[[Category: Biotin binding protein]]
	[[Category: Protein binding]]		[[Category: Protein binding]]

OCA at 23:34, 28 September 2014

OCA — Sun, 28 Sep 2014 23:34:44 GMT

←Older revision		Revision as of 23:34, 28 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1swu.~~png\|left~~\|200px]]	+	==STREPTAVIDIN MUTANT Y43F==
		+	<StructureSection load='1swu' size='340' side='right' caption='[[1swu]], [[Resolution\|resolution]] 1.14Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1swu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SWU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=MRD:(4R)-2-METHYLPENTANE-2,4-DIOL'>MRD</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1swu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1swu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1swu RCSB], [http://www.ebi.ac.uk/pdbsum/1swu PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sw/1swu_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The streptavidin-biotin system is an example of a high-affinity protein-ligand pair (Ka approximately 10(13) mol-1). The thermodynamic and structural properties have been extensively studied as a model system for protein-ligand interactions. Here, the X-ray crystal structure of a streptavidin mutant of a residue hydrogen bonding to biotin [Tyr43Phe (Y43F)] is reported at atomic resolution (1.14 A). The biotin-free structure was refined with anisotropic displacement parameters (SHELXL97 program package). The high-resolution data also allowed interpretation of side-chain and residue disorder in 41 residues where alternate conformations were refined. The Y43F mutation is unambiguously observed in difference maps, although only a single O atom per monomer is altered. The atomic resolution enabled the identification of 2-methyl-2, 4-pentanediol (MPD) molecules in the biotin-binding pocket for the first time. Electron density for MPD was observed in all four subunit binding sites of the tetrameric protein. This was not possible with data at lower resolution (1.8-2.3 A) for wild-type streptavidin or mutants in the same crystal form using MPD in the crystallization. The impact of MPD binding on these studies is discussed.

-	~~{{STRUCTURE_1swu\| PDB=1swu \| SCENE= }}~~	+	Atomic resolution structure of biotin-free Tyr43Phe streptavidin: what is in the binding site?,Freitag S, Le Trong I, Klumb LA, Stayton PS, Stenkamp RE Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1118-26. PMID:10329773<ref>PMID:10329773</ref>

-	~~===STREPTAVIDIN MUTANT Y43F===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10329773}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1swu]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_avidinii Streptomyces avidinii]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA].~~	+

	==See Also==		==See Also==
	*[[Avidin\|Avidin]]		*[[Avidin\|Avidin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010329773<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: Freitag, S.]]		[[Category: Freitag, S.]]

OCA at 19:59, 20 October 2012

OCA — Sat, 20 Oct 2012 19:59:34 GMT

←Older revision		Revision as of 19:59, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1swu]] is a 4 chain structure ~~of [[Avidin]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA].	+	[[1swu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:010329773~~</ref><ref group="xtra">PMID:012925786~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010329773</ref><references group="xtra"/>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: Freitag, S.]]		[[Category: Freitag, S.]]

OCA: Protected "1swu" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 09:05:55 GMT

Protected "1swu" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:05, 27 July 2012

OCA at 09:05, 27 July 2012

OCA — Fri, 27 Jul 2012 09:05:54 GMT

←Older revision		Revision as of 09:05, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1swu.png\|left\|200px]]		[[Image:1swu.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1swu", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1swu\| PDB=1swu \| SCENE= }}		{{STRUCTURE_1swu\| PDB=1swu \| SCENE= }}

	===STREPTAVIDIN MUTANT Y43F===		===STREPTAVIDIN MUTANT Y43F===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_10329773}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 10329773 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_10329773}}		{{ABSTRACT_PUBMED_10329773}}

	==About this Structure==		==About this Structure==
-	~~1SWU~~ is a 4 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA].	+	[[1swu]] is a 4 chain structure of [[Avidin]] with sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SWU OCA].
		+
		+	==See Also==
		+	*[[Avidin\|Avidin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~10329773~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:010329773</ref><ref group="xtra">PMID:012925786</ref><references group="xtra"/>
	[[Category: Streptomyces avidinii]]		[[Category: Streptomyces avidinii]]
	[[Category: Freitag, S.]]		[[Category: Freitag, S.]]
Line 32:		Line 23:
	[[Category: Biotin binding protein]]		[[Category: Biotin binding protein]]
	[[Category: Protein binding]]		[[Category: Protein binding]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 02:27:54 2009''