1sxu - Revision history

OCA at 06:21, 23 August 2023

OCA — Wed, 23 Aug 2023 06:21:37 GMT

←Older revision		Revision as of 06:21, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1sxu' size='340' side='right'caption='[[1sxu]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1sxu' size='340' side='right'caption='[[1sxu]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sxu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Rhopr Rhopr~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SXU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sxu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SXU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~, ~~<scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.4Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1ikj\|1ikj]]~~, ~~[[1sxw\|1sxw]], [[1sxx\|1sxx]], [[1sxy\|1sxy]], [[1sy0\|1sy0]], [[1sy1\|1sy1]], [[1sy2\|1sy2]], [[1sy3\|1sy3]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxu OCA], [https://pdbe.org/1sxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sxu RCSB], [https://www.ebi.ac.uk/pdbsum/1sxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sxu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxu OCA], [https://pdbe.org/1sxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sxu RCSB], [https://www.ebi.ac.uk/pdbsum/1sxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sxu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/NP4_RHOPR NP4_RHOPR]] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity).	+	[https://www.uniprot.org/uniprot/NP4_RHOPR NP4_RHOPR] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Rhopr~~]]	+	[[Category: Rhodnius prolixus]]
-	[[Category: Andersen~~, J F~~]]	+	[[Category: Andersen JF]]
-	[[Category: Maes~~, E M~~]]	+	[[Category: Maes EM]]
-	[[Category: Montfort~~, W R~~]]	+	[[Category: Montfort WR]]
-	[[Category: Shepley, D]]	+	[[Category: Shepley D]]
-	[[Category: Weichsel, A]]	+	[[Category: Weichsel A]]
-	~~[[Category: Beta barrel]]~~	+
-	~~[[Category: Ferric heme]]~~	+
-	~~[[Category: Imidazole]]~~	+
-	~~[[Category: Lipocalin]]~~	+
-	~~[[Category: Transport protein~~]]	+

OCA at 09:02, 21 April 2021

OCA — Wed, 21 Apr 2021 09:02:14 GMT

←Older revision		Revision as of 09:02, 21 April 2021
Line 3:		Line 3:
	<StructureSection load='1sxu' size='340' side='right'caption='[[1sxu]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1sxu' size='340' side='right'caption='[[1sxu]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sxu]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1SXU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sxu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SXU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ikj\|1ikj]], [[1sxw\|1sxw]], [[1sxx\|1sxx]], [[1sxy\|1sxy]], [[1sy0\|1sy0]], [[1sy1\|1sy1]], [[1sy2\|1sy2]], [[1sy3\|1sy3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ikj\|1ikj]], [[1sxw\|1sxw]], [[1sxx\|1sxx]], [[1sxy\|1sxy]], [[1sy0\|1sy0]], [[1sy1\|1sy1]], [[1sy2\|1sy2]], [[1sy3\|1sy3]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1sxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxu OCA], [~~http~~://pdbe.org/1sxu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1sxu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1sxu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1sxu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxu OCA], [https://pdbe.org/1sxu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sxu RCSB], [https://www.ebi.ac.uk/pdbsum/1sxu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sxu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/NP4_RHOPR NP4_RHOPR]] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity).	+	[[https://www.uniprot.org/uniprot/NP4_RHOPR NP4_RHOPR]] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 13:06, 17 July 2019

OCA — Wed, 17 Jul 2019 13:06:29 GMT

←Older revision		Revision as of 13:06, 17 July 2019
Line 1:		Line 1:

	==1.4 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus Complexed with Imidazole==		==1.4 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus Complexed with Imidazole==
-	<StructureSection load='1sxu' size='340' side='right' caption='[[1sxu]], [[Resolution\|resolution]] 1.40Å' scene=''>	+	<StructureSection load='1sxu' size='340' side='right'caption='[[1sxu]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1sxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SXU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1sxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SXU FirstGlance]. <br>
Line 14:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sx/1sxu_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sx/1sxu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1sxu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1sxu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Nitrophorin\|Nitrophorin]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Rhopr]]		[[Category: Rhopr]]
	[[Category: Andersen, J F]]		[[Category: Andersen, J F]]

OCA at 08:17, 12 October 2017

OCA — Thu, 12 Oct 2017 08:17:56 GMT

←Older revision		Revision as of 08:17, 12 October 2017
Line 1:		Line 1:
		+
	==1.4 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus Complexed with Imidazole==		==1.4 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus Complexed with Imidazole==
	<StructureSection load='1sxu' size='340' side='right' caption='[[1sxu]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1sxu' size='340' side='right' caption='[[1sxu]], [[Resolution\|resolution]] 1.40Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ikj\|1ikj]], [[1sxw\|1sxw]], [[1sxx\|1sxx]], [[1sxy\|1sxy]], [[1sy0\|1sy0]], [[1sy1\|1sy1]], [[1sy2\|1sy2]], [[1sy3\|1sy3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ikj\|1ikj]], [[1sxw\|1sxw]], [[1sxx\|1sxx]], [[1sxy\|1sxy]], [[1sy0\|1sy0]], [[1sy1\|1sy1]], [[1sy2\|1sy2]], [[1sy3\|1sy3]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxu OCA], [http://pdbe.org/1sxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sxu RCSB], [http://www.ebi.ac.uk/pdbsum/1sxu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxu OCA], [http://pdbe.org/1sxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sxu RCSB], [http://www.ebi.ac.uk/pdbsum/1sxu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1sxu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 09:08, 8 February 2016

OCA — Mon, 08 Feb 2016 09:08:13 GMT

←Older revision		Revision as of 09:08, 8 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sxu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:02, 10 September 2015

OCA — Thu, 10 Sep 2015 10:02:44 GMT

←Older revision		Revision as of 10:02, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1sxu' size='340' side='right' caption='[[1sxu]], [[Resolution\|resolution]] 1.40Å' scene=''>		<StructureSection load='1sxu' size='340' side='right' caption='[[1sxu]], [[Resolution\|resolution]] 1.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1sxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Rhodnius_prolixus Rhodnius prolixus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SXU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1sxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhopr Rhopr]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SXU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ikj\|1ikj]], [[1sxw\|1sxw]], [[1sxx\|1sxx]], [[1sxy\|1sxy]], [[1sy0\|1sy0]], [[1sy1\|1sy1]], [[1sy2\|1sy2]], [[1sy3\|1sy3]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ikj\|1ikj]], [[1sxw\|1sxw]], [[1sxx\|1sxx]], [[1sxy\|1sxy]], [[1sy0\|1sy0]], [[1sy1\|1sy1]], [[1sy2\|1sy2]], [[1sy3\|1sy3]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sxu RCSB], [http://www.ebi.ac.uk/pdbsum/1sxu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxu OCA], [http://pdbe.org/1sxu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sxu RCSB], [http://www.ebi.ac.uk/pdbsum/1sxu PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1sxu" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Rhodnius prolixus~~]]	+	[[Category: Rhopr]]
	[[Category: Andersen, J F]]		[[Category: Andersen, J F]]
	[[Category: Maes, E M]]		[[Category: Maes, E M]]

OCA at 22:11, 24 December 2014

OCA — Wed, 24 Dec 2014 22:11:25 GMT

←Older revision		Revision as of 22:11, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1sxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SXU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1sxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SXU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ikj\|1ikj]], [[1sxw\|1sxw]], [[1sxx\|1sxx]], [[1sxy\|1sxy]], [[1sy0\|1sy0]], [[1sy1\|1sy1]], [[1sy2\|1sy2]], [[1sy3\|1sy3]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ikj\|1ikj]], [[1sxw\|1sxw]], [[1sxx\|1sxx]], [[1sxy\|1sxy]], [[1sy0\|1sy0]], [[1sy1\|1sy1]], [[1sy2\|1sy2]], [[1sy3\|1sy3]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sxu RCSB], [http://www.ebi.ac.uk/pdbsum/1sxu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sxu RCSB], [http://www.ebi.ac.uk/pdbsum/1sxu PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/NP4_RHOPR NP4_RHOPR]] Heme-based protein that deliver nitric oxide gas (NO) to the victim while feeding, resulting in vasodilation and inhibition of platelet aggregation. Also bind tightly to histamine, which is released by the host to induce wound healing (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Rhodnius prolixus]]		[[Category: Rhodnius prolixus]]
-	[[Category: Andersen, J F.]]	+	[[Category: Andersen, J F]]
-	[[Category: Maes, E M.]]	+	[[Category: Maes, E M]]
-	[[Category: Montfort, W R.]]	+	[[Category: Montfort, W R]]
-	[[Category: Shepley, D.]]	+	[[Category: Shepley, D]]
-	[[Category: Weichsel, A.]]	+	[[Category: Weichsel, A]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Ferric heme]]		[[Category: Ferric heme]]

OCA at 13:56, 29 September 2014

OCA — Mon, 29 Sep 2014 13:56:58 GMT

←Older revision		Revision as of 13:56, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1sxu.~~png~~\|~~left~~\|200px]]	+	==1.4 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus Complexed with Imidazole==
		+	<StructureSection load='1sxu' size='340' side='right' caption='[[1sxu]], [[Resolution\|resolution]] 1.40Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1sxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SXU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ikj\|1ikj]], [[1sxw\|1sxw]], [[1sxx\|1sxx]], [[1sxy\|1sxy]], [[1sy0\|1sy0]], [[1sy1\|1sy1]], [[1sy2\|1sy2]], [[1sy3\|1sy3]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sxu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sxu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1sxu RCSB], [http://www.ebi.ac.uk/pdbsum/1sxu PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sx/1sxu_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nitrophorins are ferric heme proteins that transport nitric oxide (NO) from blood-sucking insects to victims. NO binding is tighter at lower pH values, as found in the insect salivary gland, and weaker at the pH of the victim's tissue, facilitating NO release and subsequent vasodilation. Previous structural analyses of nitrophorin 4 (NP4) from Rhodnius prolixus revealed a substantial NO-induced conformational change involving the A-B and G-H loops, which rearrange to desolvate the distal pocket and pack nonpolar residues against the heme-ligated NO. Previous kinetic analyses revealed a slow, biphasic, and pH-dependent NO release, which was proposed to be associated with loop movements. In this study, we created NP4 mutants D30A and D30N (A-B loop), D129A/L130A (G-H loop), and T121V (distal pocket). Eight crystal structures were determined, including complexes with NO, NH(3), and imidazole, to resolutions as high as 1.0 A. The NO-induced conformational change is largely abolished in the loop mutants, but retained in T121V. Kinetic analyses using stopped-flow spectroscopy revealed the pH dependence for NO release is eliminated for D129A/L130A, considerably reduced for D30A and D30N, but retained for T121V. NO association rates were increased 2-5-fold for T121V, but were unchanged in the loop mutants. Taken together, our findings demonstrate that the pH dependency for NO release is linked to loop dynamics and that solvent reorganization is apparently rate-limiting for formation of the initial iron-nitrosyl bond. Interestingly, the multiphasic kinetic behavior of rNPs was not affected by mutations, and its cause remains unclear.

-	~~{{STRUCTURE_1sxu\| PDB=1sxu \| SCENE= }}~~	+	Role of binding site loops in controlling nitric oxide release: structure and kinetics of mutant forms of nitrophorin 4.,Maes EM, Weichsel A, Andersen JF, Shepley D, Montfort WR Biochemistry. 2004 Jun 1;43(21):6679-90. PMID:15157102<ref>PMID:15157102</ref>

-	~~===1.4 A Crystal Structure~~ of ~~D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus Complexed with Imidazole===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15157102}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1sxu]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Rhodnius_prolixus Rhodnius prolixus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015157102<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Rhodnius prolixus]]		[[Category: Rhodnius prolixus]]
	[[Category: Andersen, J F.]]		[[Category: Andersen, J F.]]

OCA: Protected "1sxu" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 11:02:44 GMT

Protected "1sxu" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:02, 5 January 2013

OCA at 11:02, 5 January 2013

OCA — Sat, 05 Jan 2013 11:02:40 GMT

←Older revision		Revision as of 11:02, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1sxu.png\|left\|200px]]		[[Image:1sxu.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1sxu", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1sxu\| PDB=1sxu \| SCENE= }}		{{STRUCTURE_1sxu\| PDB=1sxu \| SCENE= }}

	===1.4 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus Complexed with Imidazole===		===1.4 A Crystal Structure of D30N Mutant of Nitrophorin 4 from Rhodnius Prolixus Complexed with Imidazole===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15157102}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15157102 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15157102}}		{{ABSTRACT_PUBMED_15157102}}

	==About this Structure==		==About this Structure==
-	~~1SXU~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA].	+	[[1sxu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Rhodnius_prolixus Rhodnius prolixus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SXU OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15157102~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015157102</ref><references group="xtra"/>
	[[Category: Rhodnius prolixus]]		[[Category: Rhodnius prolixus]]
	[[Category: Andersen, J F.]]		[[Category: Andersen, J F.]]
Line 34:		Line 22:
	[[Category: Imidazole]]		[[Category: Imidazole]]
	[[Category: Lipocalin]]		[[Category: Lipocalin]]
-		+	[[Category: Transport protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 20:51:03 2009''~~	+