1szg - Revision history

OCA at 06:22, 23 August 2023

OCA — Wed, 23 Aug 2023 06:22:23 GMT

←Older revision		Revision as of 06:22, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1szg' size='340' side='right'caption='[[1szg]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1szg' size='340' side='right'caption='[[1szg]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1szg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_18824 Atcc 18824~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SZG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1szg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SZG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FNS:N~~-~~SULFO-FLAVIN+MONONUCLEOTIDE'>FNS</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.7Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1fcb\|1fcb]], [[1ltd\|1ltd]], [[1qcw\|1qcw]], [[1sze\|1sze]], [[1szf\|1szf]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FNS:N-SULFO-FLAVIN+MONONUCLEOTIDE'>FNS</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>CYB2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id~~=4932 ATCC 18824])</td></tr>~~	+
-	~~<tr id='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/L~~-~~lactate_dehydrogenase_(cytochrome) L~~-~~lactate dehydrogenase (cytochrome)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [https://pdbe.org/1szg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [https://www.ebi.ac.uk/pdbsum/1szg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1szg ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [https://pdbe.org/1szg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [https://www.ebi.ac.uk/pdbsum/1szg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1szg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CYB2_YEAST CYB2_YEAST]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 18824]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chapman~~, S K~~]]	+	[[Category: Saccharomyces cerevisiae]]
-	[[Category: Green~~, A J~~]]	+	[[Category: Chapman SK]]
-	[[Category: Mowat~~, C G~~]]	+	[[Category: Green AJ]]
-	[[Category: Reid~~, G A~~]]	+	[[Category: Mowat CG]]
-	[[Category: Walkinshaw~~, M D~~]]	+	[[Category: Reid GA]]
-	[[Category: Wehenkel, A]]	+	[[Category: Walkinshaw MD]]
-	~~[[Category: Flavocytochrome]]~~	+	[[Category: Wehenkel A]]
-	~~[[Category: L-lactate dehydrogenase]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Sulfite~~]]	+

OCA at 15:36, 27 October 2021

OCA — Wed, 27 Oct 2021 15:36:24 GMT

←Older revision		Revision as of 15:36, 27 October 2021
Line 3:		Line 3:
	<StructureSection load='1szg' size='340' side='right'caption='[[1szg]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1szg' size='340' side='right'caption='[[1szg]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1szg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1SZG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1szg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SZG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FNS:N-SULFO-FLAVIN+MONONUCLEOTIDE'>FNS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FNS:N-SULFO-FLAVIN+MONONUCLEOTIDE'>FNS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1fcb\|1fcb]], [[1ltd\|1ltd]], [[1qcw\|1qcw]], [[1sze\|1sze]], [[1szf\|1szf]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1fcb\|1fcb]], [[1ltd\|1ltd]], [[1qcw\|1qcw]], [[1sze\|1sze]], [[1szf\|1szf]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CYB2 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CYB2 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/L-lactate_dehydrogenase_(cytochrome) L-lactate dehydrogenase (cytochrome)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/L-lactate_dehydrogenase_(cytochrome) L-lactate dehydrogenase (cytochrome)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [~~http~~://pdbe.org/1szg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1szg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1szg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [https://pdbe.org/1szg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [https://www.ebi.ac.uk/pdbsum/1szg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1szg ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 11:37, 24 December 2020

OCA — Thu, 24 Dec 2020 11:37:17 GMT

←Older revision		Revision as of 11:37, 24 December 2020
Line 1:		Line 1:

	==A198G:L230A flavocytochrome b2 with sulfite bound==		==A198G:L230A flavocytochrome b2 with sulfite bound==
-	<StructureSection load='1szg' size='340' side='right' caption='[[1szg]], [[Resolution\|resolution]] 2.70Å' scene=''>	+	<StructureSection load='1szg' size='340' side='right'caption='[[1szg]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1szg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1SZG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1szg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1SZG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FNS:N-SULFO-FLAVIN+MONONUCLEOTIDE'>FNS</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FNS:N-SULFO-FLAVIN+MONONUCLEOTIDE'>FNS</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fcb\|1fcb]], [[1ltd\|1ltd]], [[1qcw\|1qcw]], [[1sze\|1sze]], [[1szf\|1szf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1fcb\|1fcb]], [[1ltd\|1ltd]], [[1qcw\|1qcw]], [[1sze\|1sze]], [[1szf\|1szf]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CYB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CYB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-lactate_dehydrogenase_(cytochrome) L-lactate dehydrogenase (cytochrome)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-lactate_dehydrogenase_(cytochrome) L-lactate dehydrogenase (cytochrome)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [http://pdbe.org/1szg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [http://www.ebi.ac.uk/pdbsum/1szg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1szg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [http://pdbe.org/1szg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [http://www.ebi.ac.uk/pdbsum/1szg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1szg ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 31:		Line 31:

	==See Also==		==See Also==
-	*[[Flavocytochrome\|Flavocytochrome]]	+	*[[Lactate dehydrogenase 3D structures\|Lactate dehydrogenase 3D structures]]
-	*[[Lactate ~~Dehydrogenase~~\|Lactate ~~Dehydrogenase~~]]	+
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 18824]]		[[Category: Atcc 18824]]
		+	[[Category: Large Structures]]
	[[Category: Chapman, S K]]		[[Category: Chapman, S K]]
	[[Category: Green, A J]]		[[Category: Green, A J]]

OCA at 07:57, 14 March 2018

OCA — Wed, 14 Mar 2018 07:57:15 GMT

←Older revision		Revision as of 07:57, 14 March 2018
Line 1:		Line 1:
		+
	==A198G:L230A flavocytochrome b2 with sulfite bound==		==A198G:L230A flavocytochrome b2 with sulfite bound==
	<StructureSection load='1szg' size='340' side='right' caption='[[1szg]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1szg' size='340' side='right' caption='[[1szg]], [[Resolution\|resolution]] 2.70Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CYB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CYB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-lactate_dehydrogenase_(cytochrome) L-lactate dehydrogenase (cytochrome)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-lactate_dehydrogenase_(cytochrome) L-lactate dehydrogenase (cytochrome)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [http://pdbe.org/1szg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [http://www.ebi.ac.uk/pdbsum/1szg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [http://pdbe.org/1szg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [http://www.ebi.ac.uk/pdbsum/1szg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1szg ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 13:		Line 14:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sz/1szg_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sz/1szg_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1szg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:33, 12 September 2015

OCA — Sat, 12 Sep 2015 02:33:28 GMT

←Older revision		Revision as of 02:33, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='1szg' size='340' side='right' caption='[[1szg]], [[Resolution\|resolution]] 2.70Å' scene=''>		<StructureSection load='1szg' size='340' side='right' caption='[[1szg]], [[Resolution\|resolution]] 2.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1szg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Saccharomyces_cerevisiae Saccharomyces cerevisiae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SZG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1szg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_18824 Atcc 18824]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SZG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FNS:N-SULFO-FLAVIN+MONONUCLEOTIDE'>FNS</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FNS:N-SULFO-FLAVIN+MONONUCLEOTIDE'>FNS</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fcb\|1fcb]], [[1ltd\|1ltd]], [[1qcw\|1qcw]], [[1sze\|1sze]], [[1szf\|1szf]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fcb\|1fcb]], [[1ltd\|1ltd]], [[1qcw\|1qcw]], [[1sze\|1sze]], [[1szf\|1szf]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CYB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ~~Saccharomyces cerevisiae~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CYB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-lactate_dehydrogenase_(cytochrome) L-lactate dehydrogenase (cytochrome)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-lactate_dehydrogenase_(cytochrome) L-lactate dehydrogenase (cytochrome)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [http://www.ebi.ac.uk/pdbsum/1szg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [http://pdbe.org/1szg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [http://www.ebi.ac.uk/pdbsum/1szg PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 27:		Line 27:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1szg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Saccharomyces cerevisiae~~]]	+	[[Category: Atcc 18824]]
	[[Category: Chapman, S K]]		[[Category: Chapman, S K]]
	[[Category: Green, A J]]		[[Category: Green, A J]]

OCA at 08:19, 6 January 2015

OCA — Tue, 06 Jan 2015 08:19:12 GMT

←Older revision		Revision as of 08:19, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1szg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SZG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1szg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SZG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FNS:N-SULFO-FLAVIN+MONONUCLEOTIDE'>FNS</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FNS:N-SULFO-FLAVIN+MONONUCLEOTIDE'>FNS</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fcb\|1fcb]], [[1ltd\|1ltd]], [[1qcw\|1qcw]], [[1sze\|1sze]], [[1szf\|1szf]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fcb\|1fcb]], [[1ltd\|1ltd]], [[1qcw\|1qcw]], [[1sze\|1sze]], [[1szf\|1szf]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CYB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CYB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-lactate_dehydrogenase_(cytochrome) L-lactate dehydrogenase (cytochrome)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-lactate_dehydrogenase_(cytochrome) L-lactate dehydrogenase (cytochrome)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [http://www.ebi.ac.uk/pdbsum/1szg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [http://www.ebi.ac.uk/pdbsum/1szg PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
-	[[Category: Chapman, S K.]]	+	[[Category: Chapman, S K]]
-	[[Category: Green, A J.]]	+	[[Category: Green, A J]]
-	[[Category: Mowat, C G.]]	+	[[Category: Mowat, C G]]
-	[[Category: Reid, G A.]]	+	[[Category: Reid, G A]]
-	[[Category: Walkinshaw, M D.]]	+	[[Category: Walkinshaw, M D]]
-	[[Category: Wehenkel, A.]]	+	[[Category: Wehenkel, A]]
	[[Category: Flavocytochrome]]		[[Category: Flavocytochrome]]
	[[Category: L-lactate dehydrogenase]]		[[Category: L-lactate dehydrogenase]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Sulfite]]		[[Category: Sulfite]]

OCA at 00:07, 29 September 2014

OCA — Mon, 29 Sep 2014 00:07:00 GMT

←Older revision		Revision as of 00:07, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1szg.~~png~~\|~~left~~\|200px]]	+	==A198G:L230A flavocytochrome b2 with sulfite bound==
		+	<StructureSection load='1szg' size='340' side='right' caption='[[1szg]], [[Resolution\|resolution]] 2.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1szg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SZG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FNS:N-SULFO-FLAVIN+MONONUCLEOTIDE'>FNS</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fcb\|1fcb]], [[1ltd\|1ltd]], [[1qcw\|1qcw]], [[1sze\|1sze]], [[1szf\|1szf]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CYB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/L-lactate_dehydrogenase_(cytochrome) L-lactate dehydrogenase (cytochrome)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.2.3 1.1.2.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1szg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1szg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1szg RCSB], [http://www.ebi.ac.uk/pdbsum/1szg PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sz/1szg_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Flavocytochrome b(2) from Saccharomyces cerevisiae is a l-lactate/cytochrome c oxidoreductase belonging to a large family of 2-hydroxyacid-dependent flavoenzymes. The crystal structure of the enzyme, with pyruvate bound at the active site, has been determined [Xia, Z.-X., and Mathews, F. S. (1990) J. Mol. Biol. 212, 837-863]. The authors indicate that the methyl group of pyruvate is in close contact with Ala198 and Leu230. These two residues are not well-conserved throughout the family of (S)-2-hydroxy acid oxidases/dehydrogenases. Thus, to probe substrate specificity in flavocytochrome b(2), these residues have been substituted by glycine and alanine, respectively. Kinetic studies on the L230A mutant enzyme and the A198G/L230A double mutant enzyme indicate a change in substrate selectivity for the enzyme toward larger (S)-2-hydroxy acids. In particular, the L230A enzyme is more efficient at utilizing (S)-2-hydroxyoctanoate by a factor of 40 as compared to the wild-type enzyme [Daff, S., Manson, F. D. C., Reid, G. A., and Chapman, S. K. (1994) Biochem. J. 301, 829-834], and the A198G/L230A double mutant enzyme is 6-fold more efficient with the aromatic substrate l-mandelate than it is with l-lactate [Sinclair, R., Reid, G. A., and Chapman, S. K. (1998) Biochem. J. 333, 117-120]. To complement these solution studies, we have solved the structure of the A198G/L230A enzyme in complex with pyruvate and as the FMN-sulfite adduct (both to 2.7 A resolution). We have also obtained the structure of the L230A mutant enzyme in complex with phenylglyoxylate (the product of mandelate oxidation) to 3.0 A resolution. These structures reveal the increased active-site volume available for binding larger substrates, while also confirming that the integrity of the interactions important for catalysis is maintained. In addition to this, the mode of binding of the bulky phenylglyoxylate at the active site is in accordance with the operation of a hydride transfer mechanism for substrate oxidation/flavin reduction in flavocytochrome b(2), whereas a mechanism involving the formation of a carbanion intermediate would appear to be sterically prohibited.

-	~~{{STRUCTURE_1szg\| PDB=1szg \| SCENE= }}~~	+	Altered substrate specificity in flavocytochrome b2: structural insights into the mechanism of L-lactate dehydrogenation.,Mowat CG, Wehenkel A, Green AJ, Walkinshaw MD, Reid GA, Chapman SK Biochemistry. 2004 Jul 27;43(29):9519-26. PMID:15260495<ref>PMID:15260495</ref>

-	~~===A198G:L230A flavocytochrome b2 with sulfite bound===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15260495}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1szg]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA].~~	+

	==See Also==		==See Also==
	*[[Flavocytochrome\|Flavocytochrome]]		*[[Flavocytochrome\|Flavocytochrome]]
	*[[Lactate Dehydrogenase\|Lactate Dehydrogenase]]		*[[Lactate Dehydrogenase\|Lactate Dehydrogenase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015260495<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Chapman, S K.]]		[[Category: Chapman, S K.]]

OCA at 22:32, 20 October 2012

OCA — Sat, 20 Oct 2012 22:32:55 GMT

←Older revision		Revision as of 22:32, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1szg]] is a 2 chain structure ~~of [[Flavocytochrome]] and [[Lactate Dehydrogenase]]~~ with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA].	+	[[1szg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA].

	==See Also==		==See Also==

OCA: Protected "1szg" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 16:40:51 GMT

Protected "1szg" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:40, 27 July 2012

OCA at 16:40, 27 July 2012

OCA — Fri, 27 Jul 2012 16:40:50 GMT

←Older revision		Revision as of 16:40, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1szg.png\|left\|200px]]		[[Image:1szg.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1szg", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1szg\| PDB=1szg \| SCENE= }}		{{STRUCTURE_1szg\| PDB=1szg \| SCENE= }}

	===A198G:L230A flavocytochrome b2 with sulfite bound===		===A198G:L230A flavocytochrome b2 with sulfite bound===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15260495}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15260495 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15260495}}		{{ABSTRACT_PUBMED_15260495}}

	==About this Structure==		==About this Structure==
-	~~1SZG~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA].	+	[[1szg]] is a 2 chain structure of [[Flavocytochrome]] and [[Lactate Dehydrogenase]] with sequence from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SZG OCA].
		+
		+	==See Also==
		+	*[[Flavocytochrome\|Flavocytochrome]]
		+	*[[Lactate Dehydrogenase\|Lactate Dehydrogenase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15260495~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015260495</ref><references group="xtra"/>
	[[Category: Saccharomyces cerevisiae]]		[[Category: Saccharomyces cerevisiae]]
	[[Category: Chapman, S K.]]		[[Category: Chapman, S K.]]
Line 33:		Line 25:
	[[Category: Flavocytochrome]]		[[Category: Flavocytochrome]]
	[[Category: L-lactate dehydrogenase]]		[[Category: L-lactate dehydrogenase]]
		+	[[Category: Oxidoreductase]]
	[[Category: Sulfite]]		[[Category: Sulfite]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 10:05:38 2009''