1t0w - Revision history

OCA at 08:37, 1 May 2024

OCA — Wed, 01 May 2024 08:37:17 GMT

←Older revision		Revision as of 08:37, 1 May 2024
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	==25 NMR structures of Truncated Hevein of 32 aa (Hevein-32) complex with N,N,N-triacetylglucosamina==		==25 NMR structures of Truncated Hevein of 32 aa (Hevein-32) complex with N,N,N-triacetylglucosamina==
-	<StructureSection load='1t0w' size='340' side='right'caption='[[1t0w~~]], [[NMR_Ensembles_of_Models \| 25 NMR models~~]]' scene=''>	+	<StructureSection load='1t0w' size='340' side='right'caption='[[1t0w]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t0w]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T0W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t0w]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Hevea_brasiliensis Hevea brasiliensis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T0W FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~NH2~~:~~AMINO+GROUP~~'>~~NH2~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=PRD_900017:triacetyl-beta-chitotriose'>PRD_900017</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1hev\|1hev]], [[1q9b\|1q9b]], [[1mmc\|1mmc]]~~</~~div~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0w OCA], [https://pdbe.org/1t0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t0w RCSB], [https://www.ebi.ac.uk/pdbsum/1t0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t0w ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0w OCA], [https://pdbe.org/1t0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t0w RCSB], [https://www.ebi.ac.uk/pdbsum/1t0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t0w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HEVE_HEVBR HEVE_HEVBR]] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin. Can inhibit fungal growth.	+	[https://www.uniprot.org/uniprot/HEVE_HEVBR HEVE_HEVBR] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin. Can inhibit fungal growth.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t0w ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t0w ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	HEV32, a 32-residue, truncated hevein lacking eleven C-terminal amino acids, was synthesized by solid-phase methodology and correctly folded with three cysteine bridge pairs. The affinities of HEV32 for small chitin fragments--in the forms of N,N',N"-triacetylchitotriose ((GlcNAc)3) (millimolar) and N,N',N",N"',N"",N""'-hexaacetylchitohexaose ((GlcNAc)6) (micromolar)--as measured by NMR and fluorescence methods, are comparable with those of native hevein. The HEV32 ligand-binding process is enthalpy driven, while entropy opposes binding. The NMR structure of ligand-bound HEV32 in aqueous solution was determined to be highly similar to the NMR structure of ligand-bound hevein. Solvated molecular-dynamics simulations were performed in order to monitor the changes in side-chain conformation of the binding site of HEV32 and hevein upon interaction with ligands. The calculations suggest that the Trp21 side-chain orientation of HEV32 in the free form differs from that in the bound state; this agrees with fluorescence and thermodynamic data. HEV32 provides a simple molecular model for studying protein-carbohydrate interactions and for understanding the physiological relevance of small native hevein domains lacking C-terminal residues.
-
-	NMR and modeling studies of protein-carbohydrate interactions: synthesis, three-dimensional structure, and recognition properties of a minimum hevein domain with binding affinity for chitooligosaccharides.,Aboitiz N, Vila-Perello M, Groves P, Asensio JL, Andreu D, Canada FJ, Jimenez-Barbero J Chembiochem. 2004 Sep 6;5(9):1245-55. PMID:15368576<ref>PMID:15368576</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1t0w" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hevea brasiliensis]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aboitiz, N]]	+	[[Category: Aboitiz N]]
-	[[Category: Andreu, D]]	+	[[Category: Andreu D]]
-	[[Category: Asensio~~, J L~~]]	+	[[Category: Asensio JL]]
-	[[Category: Canada~~, F J~~]]	+	[[Category: Canada FJ]]
-	[[Category: Groves, P]]	+	[[Category: Groves P]]
-	[[Category: Jimenez-Barbero, J]]	+	[[Category: Jimenez-Barbero J]]
-	[[Category: Vila-Perello, M]]	+	[[Category: Vila-Perello M]]
-	~~[[Category: Alpha-helix]]~~	+
-	~~[[Category: Anti-parallel beta-sheet]]~~	+
-	~~[[Category: Sugar binding protein~~]]	+

OCA at 07:40, 22 September 2021

OCA — Wed, 22 Sep 2021 07:40:20 GMT

←Older revision		Revision as of 07:40, 22 September 2021
Line 3:		Line 3:
	<StructureSection load='1t0w' size='340' side='right'caption='[[1t0w]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>		<StructureSection load='1t0w' size='340' side='right'caption='[[1t0w]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t0w]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1T0W FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t0w]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T0W FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hev\|1hev]], [[1q9b\|1q9b]], [[1mmc\|1mmc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hev\|1hev]], [[1q9b\|1q9b]], [[1mmc\|1mmc]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1t0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0w OCA], [~~http~~://pdbe.org/1t0w PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1t0w RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1t0w PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1t0w ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0w OCA], [https://pdbe.org/1t0w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t0w RCSB], [https://www.ebi.ac.uk/pdbsum/1t0w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t0w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HEVE_HEVBR HEVE_HEVBR]] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin. Can inhibit fungal growth.	+	[[https://www.uniprot.org/uniprot/HEVE_HEVBR HEVE_HEVBR]] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin. Can inhibit fungal growth.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 14:13, 18 December 2019

OCA — Wed, 18 Dec 2019 14:13:47 GMT

←Older revision		Revision as of 14:13, 18 December 2019
Line 1:		Line 1:

	==25 NMR structures of Truncated Hevein of 32 aa (Hevein-32) complex with N,N,N-triacetylglucosamina==		==25 NMR structures of Truncated Hevein of 32 aa (Hevein-32) complex with N,N,N-triacetylglucosamina==
-	<StructureSection load='1t0w' size='340' side='right' caption='[[1t0w]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>	+	<StructureSection load='1t0w' size='340' side='right'caption='[[1t0w]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1t0w]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T0W FirstGlance]. <br>		<table><tr><td colspan='2'>[[1t0w]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T0W FirstGlance]. <br>
Line 34:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Aboitiz, N]]		[[Category: Aboitiz, N]]
	[[Category: Andreu, D]]		[[Category: Andreu, D]]

OCA at 07:53, 14 March 2018

OCA — Wed, 14 Mar 2018 07:53:49 GMT

←Older revision		Revision as of 07:53, 14 March 2018
Line 1:		Line 1:
		+
	==25 NMR structures of Truncated Hevein of 32 aa (Hevein-32) complex with N,N,N-triacetylglucosamina==		==25 NMR structures of Truncated Hevein of 32 aa (Hevein-32) complex with N,N,N-triacetylglucosamina==
	<StructureSection load='1t0w' size='340' side='right' caption='[[1t0w]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>		<StructureSection load='1t0w' size='340' side='right' caption='[[1t0w]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>
Line 6:		Line 7:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hev\|1hev]], [[1q9b\|1q9b]], [[1mmc\|1mmc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hev\|1hev]], [[1q9b\|1q9b]], [[1mmc\|1mmc]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0w OCA], [http://pdbe.org/1t0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t0w RCSB], [http://www.ebi.ac.uk/pdbsum/1t0w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0w OCA], [http://pdbe.org/1t0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t0w RCSB], [http://www.ebi.ac.uk/pdbsum/1t0w PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t0w ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t0/1t0w_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t0/1t0w_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:43, 10 February 2016

OCA — Wed, 10 Feb 2016 06:43:49 GMT

←Older revision		Revision as of 06:43, 10 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t0w ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 06:22, 11 September 2015

OCA — Fri, 11 Sep 2015 06:22:49 GMT

←Older revision		Revision as of 06:22, 11 September 2015
Line 6:		Line 6:
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hev\|1hev]], [[1q9b\|1q9b]], [[1mmc\|1mmc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hev\|1hev]], [[1q9b\|1q9b]], [[1mmc\|1mmc]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t0w RCSB], [http://www.ebi.ac.uk/pdbsum/1t0w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0w OCA], [http://pdbe.org/1t0w PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t0w RCSB], [http://www.ebi.ac.uk/pdbsum/1t0w PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1t0w" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:30, 25 December 2014

OCA — Thu, 25 Dec 2014 12:30:58 GMT

←Older revision		Revision as of 12:30, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1t0w]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T0W FirstGlance]. <br>		<table><tr><td colspan='2'>[[1t0w]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T0W FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hev\|1hev]], [[1q9b\|1q9b]], [[1mmc\|1mmc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hev\|1hev]], [[1q9b\|1q9b]], [[1mmc\|1mmc]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t0w RCSB], [http://www.ebi.ac.uk/pdbsum/1t0w PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t0w RCSB], [http://www.ebi.ac.uk/pdbsum/1t0w PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HEVE_HEVBR HEVE_HEVBR]] N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin. Can inhibit fungal growth.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 30:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aboitiz, N.]]	+	[[Category: Aboitiz, N]]
-	[[Category: Andreu, D.]]	+	[[Category: Andreu, D]]
-	[[Category: Asensio, J L.]]	+	[[Category: Asensio, J L]]
-	[[Category: Canada, F J.]]	+	[[Category: Canada, F J]]
-	[[Category: Groves, P.]]	+	[[Category: Groves, P]]
-	[[Category: Jimenez-Barbero, J.]]	+	[[Category: Jimenez-Barbero, J]]
-	[[Category: Vila-Perello, M.]]	+	[[Category: Vila-Perello, M]]
	[[Category: Alpha-helix]]		[[Category: Alpha-helix]]
	[[Category: Anti-parallel beta-sheet]]		[[Category: Anti-parallel beta-sheet]]
	[[Category: Sugar binding protein]]		[[Category: Sugar binding protein]]

OCA at 15:11, 29 September 2014

OCA — Mon, 29 Sep 2014 15:11:16 GMT

←Older revision		Revision as of 15:11, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1t0w.~~png~~\|~~left~~\|200px]]	+	==25 NMR structures of Truncated Hevein of 32 aa (Hevein-32) complex with N,N,N-triacetylglucosamina==
		+	<StructureSection load='1t0w' size='340' side='right' caption='[[1t0w]], [[NMR_Ensembles_of_Models \| 25 NMR models]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1t0w]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T0W FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hev\|1hev]], [[1q9b\|1q9b]], [[1mmc\|1mmc]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t0w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t0w OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t0w RCSB], [http://www.ebi.ac.uk/pdbsum/1t0w PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t0/1t0w_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	HEV32, a 32-residue, truncated hevein lacking eleven C-terminal amino acids, was synthesized by solid-phase methodology and correctly folded with three cysteine bridge pairs. The affinities of HEV32 for small chitin fragments--in the forms of N,N',N"-triacetylchitotriose ((GlcNAc)3) (millimolar) and N,N',N",N"',N"",N""'-hexaacetylchitohexaose ((GlcNAc)6) (micromolar)--as measured by NMR and fluorescence methods, are comparable with those of native hevein. The HEV32 ligand-binding process is enthalpy driven, while entropy opposes binding. The NMR structure of ligand-bound HEV32 in aqueous solution was determined to be highly similar to the NMR structure of ligand-bound hevein. Solvated molecular-dynamics simulations were performed in order to monitor the changes in side-chain conformation of the binding site of HEV32 and hevein upon interaction with ligands. The calculations suggest that the Trp21 side-chain orientation of HEV32 in the free form differs from that in the bound state; this agrees with fluorescence and thermodynamic data. HEV32 provides a simple molecular model for studying protein-carbohydrate interactions and for understanding the physiological relevance of small native hevein domains lacking C-terminal residues.

-	~~{{STRUCTURE_1t0w\| PDB=1t0w \| SCENE= }}~~	+	NMR and modeling studies of protein-carbohydrate interactions: synthesis, three-dimensional structure, and recognition properties of a minimum hevein domain with binding affinity for chitooligosaccharides.,Aboitiz N, Vila-Perello M, Groves P, Asensio JL, Andreu D, Canada FJ, Jimenez-Barbero J Chembiochem. 2004 Sep 6;5(9):1245-55. PMID:15368576<ref>PMID:15368576</ref>

-	~~===25 NMR structures of Truncated Hevein of 32 aa (Hevein-32) complex with N~~,~~N,N-triacetylglucosamina===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15368576}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1t0w]] is~~ a ~~1 chain structure~~. ~~Full experimental information is available from [http://oca~~.~~weizmann~~.~~ac.il~~/~~oca-bin/ocashort?id=1T0W OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015368576<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Aboitiz, N.]]		[[Category: Aboitiz, N.]]
	[[Category: Andreu, D.]]		[[Category: Andreu, D.]]

OCA: Protected "1t0w" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 12:52:43 GMT

Protected "1t0w" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:52, 5 January 2013

OCA at 12:52, 5 January 2013

OCA — Sat, 05 Jan 2013 12:52:40 GMT

←Older revision		Revision as of 12:52, 5 January 2013
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-	{{Seed}}
	[[Image:1t0w.png\|left\|200px]]		[[Image:1t0w.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1t0w", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1t0w\| PDB=1t0w \| SCENE= }}		{{STRUCTURE_1t0w\| PDB=1t0w \| SCENE= }}

	===25 NMR structures of Truncated Hevein of 32 aa (Hevein-32) complex with N,N,N-triacetylglucosamina===		===25 NMR structures of Truncated Hevein of 32 aa (Hevein-32) complex with N,N,N-triacetylglucosamina===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15368576}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15368576 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15368576}}		{{ABSTRACT_PUBMED_15368576}}

	==About this Structure==		==About this Structure==
-	~~1T0W~~ is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA].	+	[[1t0w]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T0W OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15368576~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015368576</ref><references group="xtra"/>
	[[Category: Aboitiz, N.]]		[[Category: Aboitiz, N.]]
	[[Category: Andreu, D.]]		[[Category: Andreu, D.]]
Line 33:		Line 21:
	[[Category: Alpha-helix]]		[[Category: Alpha-helix]]
	[[Category: Anti-parallel beta-sheet]]		[[Category: Anti-parallel beta-sheet]]
-		+	[[Category: Sugar binding protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 16:14:20 2009''~~	+