1t1i - Revision history

OCA at 08:35, 14 February 2024

OCA — Wed, 14 Feb 2024 08:35:27 GMT

←Older revision		Revision as of 08:35, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1t1i' size='340' side='right'caption='[[1t1i]], [[Resolution\|resolution]] 1.28Å' scene=''>		<StructureSection load='1t1i' size='340' side='right'caption='[[1t1i]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t1i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bacillus_novosp~~.~~_mn~~-32 Bacillus ~~novosp~~. mn-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T1I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t1i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_sp._MN-32 Bacillus sp. MN-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T1I FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.28Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1gt9\|1gt9]], [[1gtg\|1gtg]], [[1gtj\|1gtj]], [[1gtl\|1gtl]], [[1t1e\|1t1e]], [[1t1g\|1t1g]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">KSCP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198803 Bacillus novosp. MN-32])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [https://pdbe.org/1t1i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [https://www.ebi.ac.uk/pdbsum/1t1i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [https://pdbe.org/1t1i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [https://www.ebi.ac.uk/pdbsum/1t1i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1i ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q8RR56_9BACI Q8RR56_9BACI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t1i ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t1i ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Kumamolisin, an extracellular proteinase derived from an acido/thermophilic Bacillus, belongs to the sedolisin family of endopeptidases characterized by a subtilisin-like fold and a Ser-Glu-Asp catalytic triad. In kumamolisin, the Asp82 carboxylate hydrogen bonds to Glu32-Trp129, which might act as a proton sink stabilizing the catalytic residues. The 1.2/1.3 A crystal structures of the Glu32-->Ala and Trp129-->Ala mutants show that both mutations affect the active-site conformation, causing a 95% activity decrease. In addition, the 1.2 A crystal structure of the Ser278-->Ala mutant of pro-kumamolisin was determined. The prodomain exhibits a half-beta sandwich core docking to the catalytic domain similarly as the equivalent subtilisin prodomains in their catalytic-domain complexes. This pro-kumamolisin structure displays, for the first time, the uncleaved linker segment running across the active site and connecting the prodomain with the properly folded catalytic domain. The structure strongly points to an initial intramolecular activation cleavage in subtilases, as presumed for pro-subtilisin and pro-furin.
-
-	1.2 A crystal structure of the serine carboxyl proteinase pro-kumamolisin; structure of an intact pro-subtilase.,Comellas-Bigler M, Maskos K, Huber R, Oyama H, Oda K, Bode W Structure. 2004 Jul;12(7):1313-23. PMID:15242607<ref>PMID:15242607</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1t1i" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus ~~novosp~~. mn-32]]	+	[[Category: Bacillus sp. MN-32]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bode, W]]	+	[[Category: Bode W]]
-	[[Category: Comellas-Bigler, M]]	+	[[Category: Comellas-Bigler M]]
-	[[Category: Huber, R]]	+	[[Category: Huber R]]
-	[[Category: Maskos, K]]	+	[[Category: Maskos K]]
-	[[Category: Oda, K]]	+	[[Category: Oda K]]
-	[[Category: Oyama, H]]	+	[[Category: Oyama H]]
-	~~[[Category: Catalytic mechanism]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Kumamolisin]]~~	+
-	~~[[Category: Sedolisin]]~~	+
-	~~[[Category: Serine-carboxyl proteinase]]~~	+
-	~~[[Category: Subtilase~~]]	+

OCA at 15:37, 27 October 2021

OCA — Wed, 27 Oct 2021 15:37:13 GMT

←Older revision		Revision as of 15:37, 27 October 2021
Line 3:		Line 3:
	<StructureSection load='1t1i' size='340' side='right'caption='[[1t1i]], [[Resolution\|resolution]] 1.28Å' scene=''>		<StructureSection load='1t1i' size='340' side='right'caption='[[1t1i]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t1i]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bacillus_novosp._mn-32 Bacillus novosp. mn-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1T1I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t1i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_novosp._mn-32 Bacillus novosp. mn-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T1I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gt9\|1gt9]], [[1gtg\|1gtg]], [[1gtj\|1gtj]], [[1gtl\|1gtl]], [[1t1e\|1t1e]], [[1t1g\|1t1g]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gt9\|1gt9]], [[1gtg\|1gtg]], [[1gtj\|1gtj]], [[1gtl\|1gtl]], [[1t1e\|1t1e]], [[1t1g\|1t1g]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KSCP ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198803 Bacillus novosp. MN-32])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KSCP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198803 Bacillus novosp. MN-32])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [~~http~~://pdbe.org/1t1i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1t1i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1i ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [https://pdbe.org/1t1i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [https://www.ebi.ac.uk/pdbsum/1t1i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1i ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 11:38, 24 December 2020

OCA — Thu, 24 Dec 2020 11:38:57 GMT

←Older revision		Revision as of 11:38, 24 December 2020
Line 1:		Line 1:

	==High Resolution Crystal Structure of Mutant W129A of Kumamolisin, a Sedolisin Type Proteinase (previously called Kumamolysin or KSCP)==		==High Resolution Crystal Structure of Mutant W129A of Kumamolisin, a Sedolisin Type Proteinase (previously called Kumamolysin or KSCP)==
-	<StructureSection load='1t1i' size='340' side='right' caption='[[1t1i]], [[Resolution\|resolution]] 1.28Å' scene=''>	+	<StructureSection load='1t1i' size='340' side='right'caption='[[1t1i]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t1i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_novosp._mn-32 Bacillus novosp. mn-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1T1I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t1i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_novosp._mn-32 Bacillus novosp. mn-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1T1I FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gt9\|1gt9]], [[1gtg\|1gtg]], [[1gtj\|1gtj]], [[1gtl\|1gtl]], [[1t1e\|1t1e]], [[1t1g\|1t1g]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1gt9\|1gt9]], [[1gtg\|1gtg]], [[1gtj\|1gtj]], [[1gtl\|1gtl]], [[1t1e\|1t1e]], [[1t1g\|1t1g]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KSCP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198803 Bacillus novosp. MN-32])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KSCP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198803 Bacillus novosp. MN-32])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [http://pdbe.org/1t1i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [http://www.ebi.ac.uk/pdbsum/1t1i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1i ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [http://pdbe.org/1t1i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [http://www.ebi.ac.uk/pdbsum/1t1i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1i ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	</StructureSection>		</StructureSection>
	[[Category: Bacillus novosp. mn-32]]		[[Category: Bacillus novosp. mn-32]]
		+	[[Category: Large Structures]]
	[[Category: Bode, W]]		[[Category: Bode, W]]
	[[Category: Comellas-Bigler, M]]		[[Category: Comellas-Bigler, M]]

OCA at 08:00, 14 March 2018

OCA — Wed, 14 Mar 2018 08:00:25 GMT

←Older revision		Revision as of 08:00, 14 March 2018
Line 1:		Line 1:
		+
	==High Resolution Crystal Structure of Mutant W129A of Kumamolisin, a Sedolisin Type Proteinase (previously called Kumamolysin or KSCP)==		==High Resolution Crystal Structure of Mutant W129A of Kumamolisin, a Sedolisin Type Proteinase (previously called Kumamolysin or KSCP)==
	<StructureSection load='1t1i' size='340' side='right' caption='[[1t1i]], [[Resolution\|resolution]] 1.28Å' scene=''>		<StructureSection load='1t1i' size='340' side='right' caption='[[1t1i]], [[Resolution\|resolution]] 1.28Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gt9\|1gt9]], [[1gtg\|1gtg]], [[1gtj\|1gtj]], [[1gtl\|1gtl]], [[1t1e\|1t1e]], [[1t1g\|1t1g]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gt9\|1gt9]], [[1gtg\|1gtg]], [[1gtj\|1gtj]], [[1gtl\|1gtl]], [[1t1e\|1t1e]], [[1t1g\|1t1g]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KSCP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198803 Bacillus novosp. MN-32])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KSCP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198803 Bacillus novosp. MN-32])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [http://pdbe.org/1t1i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [http://www.ebi.ac.uk/pdbsum/1t1i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [http://pdbe.org/1t1i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [http://www.ebi.ac.uk/pdbsum/1t1i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t1i ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t1/1t1i_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t1/1t1i_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 03:15, 8 February 2016

OCA — Mon, 08 Feb 2016 03:15:31 GMT

←Older revision		Revision as of 03:15, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t1i ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 07:20, 10 September 2015

OCA — Thu, 10 Sep 2015 07:20:41 GMT

←Older revision		Revision as of 07:20, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1t1i' size='340' side='right' caption='[[1t1i]], [[Resolution\|resolution]] 1.28Å' scene=''>		<StructureSection load='1t1i' size='340' side='right' caption='[[1t1i]], [[Resolution\|resolution]] 1.28Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t1i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bacillus_sp~~._mn-32 Bacillus sp. mn-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T1I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t1i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_novosp._mn-32 Bacillus novosp. mn-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T1I FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gt9\|1gt9]], [[1gtg\|1gtg]], [[1gtj\|1gtj]], [[1gtl\|1gtl]], [[1t1e\|1t1e]], [[1t1g\|1t1g]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gt9\|1gt9]], [[1gtg\|1gtg]], [[1gtj\|1gtj]], [[1gtl\|1gtl]], [[1t1e\|1t1e]], [[1t1g\|1t1g]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KSCP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198803 Bacillus sp. MN-32])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KSCP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198803 Bacillus novosp. MN-32])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [http://www.ebi.ac.uk/pdbsum/1t1i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [http://pdbe.org/1t1i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [http://www.ebi.ac.uk/pdbsum/1t1i PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1t1i" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus sp. mn-32]]	+	[[Category: Bacillus novosp. mn-32]]
	[[Category: Bode, W]]		[[Category: Bode, W]]
	[[Category: Comellas-Bigler, M]]		[[Category: Comellas-Bigler, M]]

OCA at 07:26, 6 January 2015

OCA — Tue, 06 Jan 2015 07:26:40 GMT

←Older revision		Revision as of 07:26, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1t1i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._mn-32 Bacillus sp. mn-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T1I FirstGlance]. <br>		<table><tr><td colspan='2'>[[1t1i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._mn-32 Bacillus sp. mn-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T1I FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gt9\|1gt9]], [[1gtg\|1gtg]], [[1gtj\|1gtj]], [[1gtl\|1gtl]], [[1t1e\|1t1e]], [[1t1g\|1t1g]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gt9\|1gt9]], [[1gtg\|1gtg]], [[1gtj\|1gtj]], [[1gtl\|1gtl]], [[1t1e\|1t1e]], [[1t1g\|1t1g]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KSCP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198803 Bacillus sp. MN-32])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KSCP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198803 Bacillus sp. MN-32])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [http://www.ebi.ac.uk/pdbsum/1t1i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [http://www.ebi.ac.uk/pdbsum/1t1i PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus sp. mn-32]]		[[Category: Bacillus sp. mn-32]]
-	[[Category: Bode, W.]]	+	[[Category: Bode, W]]
-	[[Category: Comellas-Bigler, M.]]	+	[[Category: Comellas-Bigler, M]]
-	[[Category: Huber, R.]]	+	[[Category: Huber, R]]
-	[[Category: Maskos, K.]]	+	[[Category: Maskos, K]]
-	[[Category: Oda, K.]]	+	[[Category: Oda, K]]
-	[[Category: Oyama, H.]]	+	[[Category: Oyama, H]]
	[[Category: Catalytic mechanism]]		[[Category: Catalytic mechanism]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 10:31, 3 October 2014

OCA — Fri, 03 Oct 2014 10:31:05 GMT

←Older revision		Revision as of 10:31, 3 October 2014
Line 1:		Line 1:
-	[[~~Image:~~1t1i.~~png~~\|~~left~~\|200px]]	+	==High Resolution Crystal Structure of Mutant W129A of Kumamolisin, a Sedolisin Type Proteinase (previously called Kumamolysin or KSCP)==
		+	<StructureSection load='1t1i' size='340' side='right' caption='[[1t1i]], [[Resolution\|resolution]] 1.28Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1t1i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._mn-32 Bacillus sp. mn-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T1I FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1gt9\|1gt9]], [[1gtg\|1gtg]], [[1gtj\|1gtj]], [[1gtl\|1gtl]], [[1t1e\|1t1e]], [[1t1g\|1t1g]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">KSCP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198803 Bacillus sp. MN-32])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t1i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t1i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t1i RCSB], [http://www.ebi.ac.uk/pdbsum/1t1i PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t1/1t1i_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Kumamolisin, an extracellular proteinase derived from an acido/thermophilic Bacillus, belongs to the sedolisin family of endopeptidases characterized by a subtilisin-like fold and a Ser-Glu-Asp catalytic triad. In kumamolisin, the Asp82 carboxylate hydrogen bonds to Glu32-Trp129, which might act as a proton sink stabilizing the catalytic residues. The 1.2/1.3 A crystal structures of the Glu32-->Ala and Trp129-->Ala mutants show that both mutations affect the active-site conformation, causing a 95% activity decrease. In addition, the 1.2 A crystal structure of the Ser278-->Ala mutant of pro-kumamolisin was determined. The prodomain exhibits a half-beta sandwich core docking to the catalytic domain similarly as the equivalent subtilisin prodomains in their catalytic-domain complexes. This pro-kumamolisin structure displays, for the first time, the uncleaved linker segment running across the active site and connecting the prodomain with the properly folded catalytic domain. The structure strongly points to an initial intramolecular activation cleavage in subtilases, as presumed for pro-subtilisin and pro-furin.

-	~~{{STRUCTURE_1t1i\| PDB=1t1i \| SCENE= }}~~	+	1.2 A crystal structure of the serine carboxyl proteinase pro-kumamolisin; structure of an intact pro-subtilase.,Comellas-Bigler M, Maskos K, Huber R, Oyama H, Oda K, Bode W Structure. 2004 Jul;12(7):1313-23. PMID:15242607<ref>PMID:15242607</ref>

-	~~===High Resolution Crystal Structure of Mutant W129A of Kumamolisin~~, a ~~Sedolisin Type Proteinase (previously called Kumamolysin or KSCP)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15242607}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1t1i]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bacillus_sp~~.~~_mn-32 Bacillus sp. mn-32]. Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015242607<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Bacillus sp. mn-32]]		[[Category: Bacillus sp. mn-32]]
	[[Category: Bode, W.]]		[[Category: Bode, W.]]

OCA: Protected "1t1i" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 11:29:03 GMT

Protected "1t1i" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:29, 5 January 2013

OCA at 11:29, 5 January 2013

OCA — Sat, 05 Jan 2013 11:29:01 GMT

←Older revision		Revision as of 11:29, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1t1i.png\|left\|200px]]		[[Image:1t1i.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1t1i", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1t1i\| PDB=1t1i \| SCENE= }}		{{STRUCTURE_1t1i\| PDB=1t1i \| SCENE= }}

	===High Resolution Crystal Structure of Mutant W129A of Kumamolisin, a Sedolisin Type Proteinase (previously called Kumamolysin or KSCP)===		===High Resolution Crystal Structure of Mutant W129A of Kumamolisin, a Sedolisin Type Proteinase (previously called Kumamolysin or KSCP)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15242607}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15242607 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15242607}}		{{ABSTRACT_PUBMED_15242607}}

	==About this Structure==		==About this Structure==
-	~~1T1I~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/~~Bacteria Bacteria~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA].	+	[[1t1i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_sp._mn-32 Bacillus sp. mn-32]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T1I OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15242607~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015242607</ref><references group="xtra"/>
-	[[Category: ~~Bacteria~~]]	+	[[Category: Bacillus sp. mn-32]]
	[[Category: Bode, W.]]		[[Category: Bode, W.]]
	[[Category: Comellas-Bigler, M.]]		[[Category: Comellas-Bigler, M.]]
Line 32:		Line 20:
	[[Category: Oyama, H.]]		[[Category: Oyama, H.]]
	[[Category: Catalytic mechanism]]		[[Category: Catalytic mechanism]]
		+	[[Category: Hydrolase]]
	[[Category: Kumamolisin]]		[[Category: Kumamolisin]]
	[[Category: Sedolisin]]		[[Category: Sedolisin]]
	[[Category: Serine-carboxyl proteinase]]		[[Category: Serine-carboxyl proteinase]]
	[[Category: Subtilase]]		[[Category: Subtilase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:24:21 2009''