1t2i - Revision history

OCA at 06:24, 23 August 2023

OCA — Wed, 23 Aug 2023 06:24:25 GMT

←Older revision		Revision as of 06:24, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1t2i' size='340' side='right'caption='[[1t2i]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='1t2i' size='340' side='right'caption='[[1t2i]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t2i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_10762 Atcc 10762~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T2I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t2i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Kitasatospora_aureofaciens Kitasatospora aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T2I FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1rgg\|1rgg]], [[1box\|1box]], [[1i70\|1i70]], [[1i8v\|1i8v]], [[1t2h\|1t2h]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.1Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">U39467 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ATCC 10762])</td></tr>~~	+
-	~~<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ribonuclease_T(~~1~~) Ribonuclease T(1)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3~~.1~~.27.3 3.1.27.3] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [https://pdbe.org/1t2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [https://www.ebi.ac.uk/pdbsum/1t2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2i ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [https://pdbe.org/1t2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [https://www.ebi.ac.uk/pdbsum/1t2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2i ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RNSA_KITAU RNSA_KITAU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 10762~~]]	+	[[Category: Kitasatospora aureofaciens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Sevcik, J]]	+	[[Category: Sevcik J]]
-	[[Category: Urbanikova, L]]	+	[[Category: Urbanikova L]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Mutant]]~~	+
-	~~[[Category: Ribonuclease~~]]	+

OCA at 15:37, 27 October 2021

OCA — Wed, 27 Oct 2021 15:37:34 GMT

←Older revision		Revision as of 15:37, 27 October 2021
Line 3:		Line 3:
	<StructureSection load='1t2i' size='340' side='right'caption='[[1t2i]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='1t2i' size='340' side='right'caption='[[1t2i]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t2i]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_10762 Atcc 10762]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1T2I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t2i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_10762 Atcc 10762]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T2I FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rgg\|1rgg]], [[1box\|1box]], [[1i70\|1i70]], [[1i8v\|1i8v]], [[1t2h\|1t2h]]</div></td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rgg\|1rgg]], [[1box\|1box]], [[1i70\|1i70]], [[1i8v\|1i8v]], [[1t2h\|1t2h]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U39467 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ATCC 10762])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U39467 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ATCC 10762])</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [~~http~~://pdbe.org/1t2i PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1t2i PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2i ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [https://pdbe.org/1t2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [https://www.ebi.ac.uk/pdbsum/1t2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2i ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]		*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	*[[Temp\|Temp]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 11:40, 24 December 2020

OCA — Thu, 24 Dec 2020 11:40:19 GMT

←Older revision		Revision as of 11:40, 24 December 2020
Line 1:		Line 1:

	==T76W mutant of RNase Sa from Streptomyces aureofaciens==		==T76W mutant of RNase Sa from Streptomyces aureofaciens==
-	<StructureSection load='1t2i' size='340' side='right' caption='[[1t2i]], [[Resolution\|resolution]] 1.10Å' scene=''>	+	<StructureSection load='1t2i' size='340' side='right'caption='[[1t2i]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t2i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10762 Atcc 10762]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1T2I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t2i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10762 Atcc 10762]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1T2I FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rgg\|1rgg]], [[1box\|1box]], [[1i70\|1i70]], [[1i8v\|1i8v]], [[1t2h\|1t2h]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1rgg\|1rgg]], [[1box\|1box]], [[1i70\|1i70]], [[1i8v\|1i8v]], [[1t2h\|1t2h]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U39467 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ATCC 10762])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U39467 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ATCC 10762])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [http://pdbe.org/1t2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [http://www.ebi.ac.uk/pdbsum/1t2i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2i ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [http://pdbe.org/1t2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [http://www.ebi.ac.uk/pdbsum/1t2i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2i ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Ribonuclease\|Ribonuclease]]	+	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
	== References ==		== References ==
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 10762]]		[[Category: Atcc 10762]]
		+	[[Category: Large Structures]]
	[[Category: Sevcik, J]]		[[Category: Sevcik, J]]
	[[Category: Urbanikova, L]]		[[Category: Urbanikova, L]]

OCA at 07:36, 14 March 2018

OCA — Wed, 14 Mar 2018 07:36:05 GMT

←Older revision		Revision as of 07:36, 14 March 2018
Line 1:		Line 1:
		+
	==T76W mutant of RNase Sa from Streptomyces aureofaciens==		==T76W mutant of RNase Sa from Streptomyces aureofaciens==
	<StructureSection load='1t2i' size='340' side='right' caption='[[1t2i]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='1t2i' size='340' side='right' caption='[[1t2i]], [[Resolution\|resolution]] 1.10Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U39467 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ATCC 10762])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U39467 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ATCC 10762])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [http://pdbe.org/1t2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [http://www.ebi.ac.uk/pdbsum/1t2i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [http://pdbe.org/1t2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [http://www.ebi.ac.uk/pdbsum/1t2i PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2i ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t2/1t2i_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t2/1t2i_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 08:37, 7 February 2016

OCA — Sun, 07 Feb 2016 08:37:56 GMT

←Older revision		Revision as of 08:37, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t2i ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 23:42, 9 September 2015

OCA — Wed, 09 Sep 2015 23:42:19 GMT

←Older revision		Revision as of 23:42, 9 September 2015
Line 2:		Line 2:
	<StructureSection load='1t2i' size='340' side='right' caption='[[1t2i]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='1t2i' size='340' side='right' caption='[[1t2i]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t2i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_aureofaciens Streptomyces aureofaciens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T2I FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t2i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_10762 Atcc 10762]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T2I FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rgg\|1rgg]], [[1box\|1box]], [[1i70\|1i70]], [[1i8v\|1i8v]], [[1t2h\|1t2h]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rgg\|1rgg]], [[1box\|1box]], [[1i70\|1i70]], [[1i8v\|1i8v]], [[1t2h\|1t2h]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U39467 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ~~Streptomyces aureofaciens~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U39467 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 ATCC 10762])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [http://www.ebi.ac.uk/pdbsum/1t2i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [http://pdbe.org/1t2i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [http://www.ebi.ac.uk/pdbsum/1t2i PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1t2i" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
-	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Streptomyces aureofaciens~~]]	+	[[Category: Atcc 10762]]
	[[Category: Sevcik, J]]		[[Category: Sevcik, J]]
	[[Category: Urbanikova, L]]		[[Category: Urbanikova, L]]

OCA at 07:51, 6 January 2015

OCA — Tue, 06 Jan 2015 07:51:45 GMT

←Older revision		Revision as of 07:51, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1t2i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T2I FirstGlance]. <br>		<table><tr><td colspan='2'>[[1t2i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T2I FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rgg\|1rgg]], [[1box\|1box]], [[1i70\|1i70]], [[1i8v\|1i8v]], [[1t2h\|1t2h]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rgg\|1rgg]], [[1box\|1box]], [[1i70\|1i70]], [[1i8v\|1i8v]], [[1t2h\|1t2h]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U39467 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 Streptomyces aureofaciens])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U39467 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 Streptomyces aureofaciens])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [http://www.ebi.ac.uk/pdbsum/1t2i PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [http://www.ebi.ac.uk/pdbsum/1t2i PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
		+	*[[Temp\|Temp]]
		+	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Streptomyces aureofaciens]]		[[Category: Streptomyces aureofaciens]]
-	[[Category: Sevcik, J.]]	+	[[Category: Sevcik, J]]
-	[[Category: Urbanikova, L.]]	+	[[Category: Urbanikova, L]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Mutant]]		[[Category: Mutant]]
	[[Category: Ribonuclease]]		[[Category: Ribonuclease]]

OCA at 21:39, 28 September 2014

OCA — Sun, 28 Sep 2014 21:39:27 GMT

←Older revision		Revision as of 21:39, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1t2i.~~png~~\|~~left~~\|200px]]	+	==T76W mutant of RNase Sa from Streptomyces aureofaciens==
		+	<StructureSection load='1t2i' size='340' side='right' caption='[[1t2i]], [[Resolution\|resolution]] 1.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1t2i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T2I FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1rgg\|1rgg]], [[1box\|1box]], [[1i70\|1i70]], [[1i8v\|1i8v]], [[1t2h\|1t2h]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">U39467 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1894 Streptomyces aureofaciens])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2i OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t2i RCSB], [http://www.ebi.ac.uk/pdbsum/1t2i PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t2/1t2i_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ribonuclease Sa (RNase Sa) contains no tryptophan (Trp) residues. We have added single Trp residues to RNase Sa at sites where Trp is found in four other microbial ribonucleases, yielding the following variants of RNase Sa: Y52W, Y55W, T76W, and Y81W. We have determined crystal structures of T76W and Y81W at 1.1 and 1.0 A resolution, respectively. We have studied the fluorescence properties and stabilities of the four variants and compared them to wild-type RNase Sa and the other ribonucleases on which they were based. Our results should help others in selecting sites for adding Trp residues to proteins. The most interesting findings are: 1), Y52W is 2.9 kcal/mol less stable than RNase Sa and the fluorescence intensity emission maximum is blue-shifted to 309 nm. Only a Trp in azurin is blue-shifted to a greater extent (308 nm). This blue shift is considerably greater than observed for Trp71 in barnase, the Trp on which Y52W is based. 2), Y55W is 2.1 kcal/mol less stable than RNase Sa and the tryptophan fluorescence is almost completely quenched. In contrast, Trp59 in RNase T1, on which Y55W is based, has a 10-fold greater fluorescence emission intensity. 3), T76W is 0.7 kcal/mol more stable than RNase Sa, indicating that the Trp side chain has more favorable interactions with the protein than the threonine side chain. The fluorescence properties of folded Y76W are similar to those of the unfolded protein, showing that the tryptophan side chain in the folded protein is largely exposed to solvent. This is confirmed by the crystal structure of the T76W which shows that the side chain of the Trp is only approximately 7% buried. 4), Y81W is 0.4 kcal/mol less stable than RNase Sa. Based on the crystal structure of Y81W, the side chain of the Trp is 87% buried. Although all of the Trp side chains in the variants contribute to the unusual positive circular dichroism band observed near 235 nm for RNase Sa, the contribution is greatest for Y81W.

-	~~{{STRUCTURE_1t2i\| PDB=1t2i \| SCENE= }}~~	+	Contribution of single tryptophan residues to the fluorescence and stability of ribonuclease Sa.,Alston RW, Urbanikova L, Sevcik J, Lasagna M, Reinhart GD, Scholtz JM, Pace CN Biophys J. 2004 Dec;87(6):4036-47. Epub 2004 Sep 17. PMID:15377518<ref>PMID:15377518</ref>

-	~~===T76W mutant~~ of ~~RNase Sa from Streptomyces aureofaciens===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15377518}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1t2i]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA].~~	+

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015377518<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Streptomyces aureofaciens]]		[[Category: Streptomyces aureofaciens]]
	[[Category: Sevcik, J.]]		[[Category: Sevcik, J.]]

OCA at 06:07, 20 October 2012

OCA — Sat, 20 Oct 2012 06:07:50 GMT

←Older revision		Revision as of 06:07, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1t2i]] is a 1 chain structure ~~of [[Ribonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA].	+	[[1t2i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2I OCA].

	==See Also==		==See Also==

OCA: Protected "1t2i" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 12:13:17 GMT

Protected "1t2i" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:13, 25 July 2012