1t2o - Revision history

OCA at 08:37, 1 May 2024

OCA — Wed, 01 May 2024 08:37:46 GMT

←Older revision		Revision as of 08:37, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='1t2o' size='340' side='right'caption='[[1t2o]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1t2o' size='340' side='right'caption='[[1t2o]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t2o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus~~ aureus~~" (rosenbach 1884) zopf 1885~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T2O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t2o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T2O FirstGlance]. <br>
-	</td></tr><tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"~~><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	~~<tr~~ id~~='related'><td class~~="~~sblockLbl~~">~~<b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max~~-~~height: 3em;'>[[1t2p\|1t2p]]~~, [[~~1t2w~~\|~~1t2w~~]]~~</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>srt ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~1280~~ "~~Micrococcus aureus~~" ~~(Rosenbach 1884) Zopf 1885])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [https://pdbe.org/1t2o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [https://www.ebi.ac.uk/pdbsum/1t2o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2o ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [https://pdbe.org/1t2o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [https://www.ebi.ac.uk/pdbsum/1t2o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2o ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9S446_STAAU Q9S446_STAAU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t2o ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t2o ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The cell wall envelope of staphylococci and other Gram-positive pathogens is coated with surface proteins that interact with human host tissues. Surface proteins of Staphylococcus aureus are covalently linked to the cell wall envelope by a mechanism requiring C-terminal sorting signals with an LPXTG motif. Sortase (SrtA) cleaves surface proteins between the threonine (T) and the glycine (G) of the LPXTG motif and catalyzes the formation of an amide bond between threonine at the C-terminal end of polypeptides and cell wall cross-bridges. The active site architecture and catalytic mechanism of sortase A has hitherto not been revealed. Here we present the crystal structures of native SrtA, of an active site mutant of SrtA, and of the mutant SrtA complexed with its substrate LPETG peptide and describe the substrate binding pocket of the enzyme. Highly conserved proline (P) and threonine (T) residues of the LPXTG motif are held in position by hydrophobic contacts, whereas the glutamic acid residue (E) at the X position points out into the solvent. The scissile T-G peptide bond is positioned between the active site Cys(184) and Arg(197) residues and at a greater distance from the imidazolium side chain of His(120). All three residues, His(120), Cys(184), and Arg(197), are conserved in sortase enzymes from Gram-positive bacteria. Comparison of the active sites of S. aureus sortase A and sortase B provides insight into substrate specificity and suggests a universal sortase-catalyzed mechanism of bacterial surface protein anchoring in Gram-positive bacteria.
-
-	Crystal structures of Staphylococcus aureus sortase A and its substrate complex.,Zong Y, Bice TW, Ton-That H, Schneewind O, Narayana SV J Biol Chem. 2004 Jul 23;279(30):31383-9. Epub 2004 Apr 26. PMID:15117963<ref>PMID:15117963</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1t2o" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bice~~, T W~~]]	+	[[Category: Staphylococcus aureus]]
-	[[Category: Narayana~~, S V~~]]	+	[[Category: Bice TW]]
-	[[Category: Schneewind, O]]	+	[[Category: Narayana SV]]
-	[[Category: Ton-That, H]]	+	[[Category: Schneewind O]]
-	[[Category: Zong, Y]]	+	[[Category: Ton-That H]]
-	~~[[Category: Beta barrel]]~~	+	[[Category: Zong Y]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Sortase]]~~	+
-	~~[[Category: Transpeptidase~~]]	+

OCA at 15:37, 27 October 2021

OCA — Wed, 27 Oct 2021 15:37:41 GMT

←Older revision		Revision as of 15:37, 27 October 2021
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	<StructureSection load='1t2o' size='340' side='right'caption='[[1t2o]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1t2o' size='340' side='right'caption='[[1t2o]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t2o]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1T2O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t2o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T2O FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1t2p\|1t2p]], [[1t2w\|1t2w]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1t2p\|1t2p]], [[1t2w\|1t2w]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srt ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srt ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [~~http~~://pdbe.org/1t2o PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1t2o PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2o ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [https://pdbe.org/1t2o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [https://www.ebi.ac.uk/pdbsum/1t2o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2o ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 11:40, 24 December 2020

OCA — Thu, 24 Dec 2020 11:40:21 GMT

←Older revision		Revision as of 11:40, 24 December 2020
Line 1:		Line 1:

	==Crystal structure of Se-SrtA, C184-Ala==		==Crystal structure of Se-SrtA, C184-Ala==
-	<StructureSection load='1t2o' size='340' side='right' caption='[[1t2o]], [[Resolution\|resolution]] 2.30Å' scene=''>	+	<StructureSection load='1t2o' size='340' side='right'caption='[[1t2o]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t2o]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1T2O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t2o]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1T2O FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2p\|1t2p]], [[1t2w\|1t2w]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1t2p\|1t2p]], [[1t2w\|1t2w]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [http://pdbe.org/1t2o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [http://www.ebi.ac.uk/pdbsum/1t2o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2o ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [http://pdbe.org/1t2o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [http://www.ebi.ac.uk/pdbsum/1t2o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2o ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 32:		Line 32:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Bice, T W]]		[[Category: Bice, T W]]
	[[Category: Narayana, S V]]		[[Category: Narayana, S V]]

OCA at 07:25, 14 March 2018

OCA — Wed, 14 Mar 2018 07:25:37 GMT

←Older revision		Revision as of 07:25, 14 March 2018
Line 1:		Line 1:
		+
	==Crystal structure of Se-SrtA, C184-Ala==		==Crystal structure of Se-SrtA, C184-Ala==
	<StructureSection load='1t2o' size='340' side='right' caption='[[1t2o]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1t2o' size='340' side='right' caption='[[1t2o]], [[Resolution\|resolution]] 2.30Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2p\|1t2p]], [[1t2w\|1t2w]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2p\|1t2p]], [[1t2w\|1t2w]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [http://pdbe.org/1t2o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [http://www.ebi.ac.uk/pdbsum/1t2o PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [http://pdbe.org/1t2o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [http://www.ebi.ac.uk/pdbsum/1t2o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t2o ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t2/1t2o_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t2/1t2o_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t2o ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:17, 11 September 2015

OCA — Fri, 11 Sep 2015 17:17:51 GMT

←Older revision		Revision as of 17:17, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1t2o' size='340' side='right' caption='[[1t2o]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='1t2o' size='340' side='right' caption='[[1t2o]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t2o]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Staphylococcus_aureus Staphylococcus~~ aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T2O FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t2o]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T2O FirstGlance]. <br>
	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2p\|1t2p]], [[1t2w\|1t2w]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2p\|1t2p]], [[1t2w\|1t2w]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 ~~Staphylococcus~~ aureus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [http://www.ebi.ac.uk/pdbsum/1t2o PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [http://pdbe.org/1t2o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [http://www.ebi.ac.uk/pdbsum/1t2o PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1t2o" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Staphylococcus aureus]]
	[[Category: Bice, T W]]		[[Category: Bice, T W]]
	[[Category: Narayana, S V]]		[[Category: Narayana, S V]]

OCA at 07:39, 6 January 2015

OCA — Tue, 06 Jan 2015 07:39:00 GMT

←Older revision		Revision as of 07:39, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1t2o]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T2O FirstGlance]. <br>		<table><tr><td colspan='2'>[[1t2o]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T2O FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2p\|1t2p]], [[1t2w\|1t2w]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2p\|1t2p]], [[1t2w\|1t2w]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [http://www.ebi.ac.uk/pdbsum/1t2o PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [http://www.ebi.ac.uk/pdbsum/1t2o PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
-	[[Category: Bice, T W.]]	+	[[Category: Bice, T W]]
-	[[Category: Narayana, S V.]]	+	[[Category: Narayana, S V]]
-	[[Category: Schneewind, O.]]	+	[[Category: Schneewind, O]]
-	[[Category: Ton-That, H.]]	+	[[Category: Ton-That, H]]
-	[[Category: Zong, Y.]]	+	[[Category: Zong, Y]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Sortase]]		[[Category: Sortase]]
	[[Category: Transpeptidase]]		[[Category: Transpeptidase]]

OCA at 18:51, 29 September 2014

OCA — Mon, 29 Sep 2014 18:51:02 GMT

←Older revision		Revision as of 18:51, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1t2o.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of Se-SrtA, C184-Ala==
		+	<StructureSection load='1t2o' size='340' side='right' caption='[[1t2o]], [[Resolution\|resolution]] 2.30Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1t2o]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T2O FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t2p\|1t2p]], [[1t2w\|1t2w]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">srt ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t2o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t2o OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t2o RCSB], [http://www.ebi.ac.uk/pdbsum/1t2o PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t2/1t2o_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cell wall envelope of staphylococci and other Gram-positive pathogens is coated with surface proteins that interact with human host tissues. Surface proteins of Staphylococcus aureus are covalently linked to the cell wall envelope by a mechanism requiring C-terminal sorting signals with an LPXTG motif. Sortase (SrtA) cleaves surface proteins between the threonine (T) and the glycine (G) of the LPXTG motif and catalyzes the formation of an amide bond between threonine at the C-terminal end of polypeptides and cell wall cross-bridges. The active site architecture and catalytic mechanism of sortase A has hitherto not been revealed. Here we present the crystal structures of native SrtA, of an active site mutant of SrtA, and of the mutant SrtA complexed with its substrate LPETG peptide and describe the substrate binding pocket of the enzyme. Highly conserved proline (P) and threonine (T) residues of the LPXTG motif are held in position by hydrophobic contacts, whereas the glutamic acid residue (E) at the X position points out into the solvent. The scissile T-G peptide bond is positioned between the active site Cys(184) and Arg(197) residues and at a greater distance from the imidazolium side chain of His(120). All three residues, His(120), Cys(184), and Arg(197), are conserved in sortase enzymes from Gram-positive bacteria. Comparison of the active sites of S. aureus sortase A and sortase B provides insight into substrate specificity and suggests a universal sortase-catalyzed mechanism of bacterial surface protein anchoring in Gram-positive bacteria.

-	~~{{STRUCTURE_1t2o\| PDB=1t2o \| SCENE= }}~~	+	Crystal structures of Staphylococcus aureus sortase A and its substrate complex.,Zong Y, Bice TW, Ton-That H, Schneewind O, Narayana SV J Biol Chem. 2004 Jul 23;279(30):31383-9. Epub 2004 Apr 26. PMID:15117963<ref>PMID:15117963</ref>

-	~~===Crystal structure of Se-SrtA~~, ~~C184-Ala===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15117963}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1t2o]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Staphylococcus_aureus Staphylococcus aureus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015117963<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Bice, T W.]]		[[Category: Bice, T W.]]

OCA: Protected "1t2o" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 11:37:52 GMT

Protected "1t2o" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:37, 5 January 2013

OCA at 11:37, 5 January 2013

OCA — Sat, 05 Jan 2013 11:37:49 GMT

←Older revision		Revision as of 11:37, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1t2o.png\|left\|200px]]		[[Image:1t2o.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1t2o", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1t2o\| PDB=1t2o \| SCENE= }}		{{STRUCTURE_1t2o\| PDB=1t2o \| SCENE= }}

	===Crystal structure of Se-SrtA, C184-Ala===		===Crystal structure of Se-SrtA, C184-Ala===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15117963}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15117963 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15117963}}		{{ABSTRACT_PUBMED_15117963}}

	==About this Structure==		==About this Structure==
-	~~1T2O~~ is a 3 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA].	+	[[1t2o]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15117963~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015117963</ref><references group="xtra"/>
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Bice, T W.]]		[[Category: Bice, T W.]]
Line 31:		Line 19:
	[[Category: Zong, Y.]]		[[Category: Zong, Y.]]
	[[Category: Beta barrel]]		[[Category: Beta barrel]]
		+	[[Category: Hydrolase]]
	[[Category: Sortase]]		[[Category: Sortase]]
	[[Category: Transpeptidase]]		[[Category: Transpeptidase]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:34:37 2009''

OCA at 02:34, 18 February 2009

OCA — Wed, 18 Feb 2009 02:34:44 GMT

←Older revision		Revision as of 02:34, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1T2O is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA].	+	1T2O is a 3 chains structure of sequences from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T2O OCA].

	==Reference==		==Reference==
-	~~Crystal structures of Staphylococcus aureus sortase A and its substrate complex., Zong Y, Bice TW, Ton-That H, Schneewind O, Narayana SV, J Biol Chem. 2004 Jul 23;279(30):31383-9. Epub 2004 Apr 26.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15117963 15117963]~~	+	<ref group="xtra">PMID:15117963</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Staphylococcus aureus]]		[[Category: Staphylococcus aureus]]
	[[Category: Bice, T W.]]		[[Category: Bice, T W.]]
Line 35:		Line 34:
	[[Category: Transpeptidase]]		[[Category: Transpeptidase]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 11~~:21:~~53 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:34:37 2009''