1t7h - Revision history

OCA at 06:39, 28 September 2022

OCA — Wed, 28 Sep 2022 06:39:58 GMT

←Older revision		Revision as of 06:39, 28 September 2022
Line 3:		Line 3:
	<StructureSection load='1t7h' size='340' side='right'caption='[[1t7h]], [[Resolution\|resolution]] 1.13Å' scene=''>		<StructureSection load='1t7h' size='340' side='right'caption='[[1t7h]], [[Resolution\|resolution]] 1.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t7h]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7H OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1T7H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t7h]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7H OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T7H FirstGlance]. <br>
-	</td></tr>~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EDN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7h OCA], [https://pdbe.org/1t7h PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t7h RCSB], [https://www.ebi.ac.uk/pdbsum/1t7h PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t7h ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1t7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7h OCA], [~~http~~://pdbe.org/1t7h PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1t7h RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1t7h PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1t7h ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/EDN1_HUMAN EDN1_HUMAN]] Endothelins are endothelium-derived vasoconstrictor peptides.	+	[[https://www.uniprot.org/uniprot/EDN1_HUMAN EDN1_HUMAN]] Endothelins are endothelium-derived vasoconstrictor peptides.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Aumelas, A]]	+	[[Category: Aumelas A]]
-	[[Category: Cerdan, R]]	+	[[Category: Cerdan R]]
-	[[Category: Chiche, L]]	+	[[Category: Chiche L]]
-	[[Category: Chino, N]]	+	[[Category: Chino N]]
-	[[Category: Dumas, C]]	+	[[Category: Dumas C]]
-	[[Category: Hoh, F]]	+	[[Category: Hoh F]]
-	[[Category: Kaas, Q]]	+	[[Category: Kaas Q]]
-	[[Category: Kobayashi, Y]]	+	[[Category: Kobayashi Y]]
-	[[Category: Kubo, S]]	+	[[Category: Kubo S]]
-	[[Category: Nishi, Y]]	+	[[Category: Nishi Y]]
-	~~[[Category: Cysteine stabilized helical motif]]~~	+
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Endothelin]]~~	+
-	~~[[Category: Salt bridge]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 11:44, 24 December 2020

OCA — Thu, 24 Dec 2020 11:44:32 GMT

←Older revision		Revision as of 11:44, 24 December 2020
Line 1:		Line 1:

	==X-ray structure of [Lys(-2)-Arg(-1)-des(17-21)]-endothelin-1 peptide==		==X-ray structure of [Lys(-2)-Arg(-1)-des(17-21)]-endothelin-1 peptide==
-	<StructureSection load='1t7h' size='340' side='right' caption='[[1t7h]], [[Resolution\|resolution]] 1.13Å' scene=''>	+	<StructureSection load='1t7h' size='340' side='right'caption='[[1t7h]], [[Resolution\|resolution]] 1.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t7h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7H OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1T7H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t7h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1T7H FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EDN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EDN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1t7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7h OCA], [http://pdbe.org/1t7h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t7h RCSB], [http://www.ebi.ac.uk/pdbsum/1t7h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t7h ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1t7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7h OCA], [http://pdbe.org/1t7h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t7h RCSB], [http://www.ebi.ac.uk/pdbsum/1t7h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t7h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Human]]		[[Category: Human]]
		+	[[Category: Large Structures]]
	[[Category: Aumelas, A]]		[[Category: Aumelas, A]]
	[[Category: Cerdan, R]]		[[Category: Cerdan, R]]

OCA at 16:03, 12 October 2017

OCA — Thu, 12 Oct 2017 16:03:47 GMT

←Older revision		Revision as of 16:03, 12 October 2017
Line 1:		Line 1:
		+
	==X-ray structure of [Lys(-2)-Arg(-1)-des(17-21)]-endothelin-1 peptide==		==X-ray structure of [Lys(-2)-Arg(-1)-des(17-21)]-endothelin-1 peptide==
	<StructureSection load='1t7h' size='340' side='right' caption='[[1t7h]], [[Resolution\|resolution]] 1.13Å' scene=''>		<StructureSection load='1t7h' size='340' side='right' caption='[[1t7h]], [[Resolution\|resolution]] 1.13Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1t7h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T7H FirstGlance]. <br>		<table><tr><td colspan='2'>[[1t7h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T7H FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EDN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EDN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7h OCA], [http://pdbe.org/1t7h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t7h RCSB], [http://www.ebi.ac.uk/pdbsum/1t7h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7h OCA], [http://pdbe.org/1t7h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t7h RCSB], [http://www.ebi.ac.uk/pdbsum/1t7h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t7h ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 02:28, 12 September 2015

OCA — Sat, 12 Sep 2015 02:28:24 GMT

←Older revision		Revision as of 02:28, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='1t7h' size='340' side='right' caption='[[1t7h]], [[Resolution\|resolution]] 1.13Å' scene=''>		<StructureSection load='1t7h' size='340' side='right' caption='[[1t7h]], [[Resolution\|resolution]] 1.13Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t7h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Homo_sapiens Homo sapiens~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T7H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t7h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T7H FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EDN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 ~~Homo sapiens~~])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EDN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t7h RCSB], [http://www.ebi.ac.uk/pdbsum/1t7h PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7h OCA], [http://pdbe.org/1t7h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t7h RCSB], [http://www.ebi.ac.uk/pdbsum/1t7h PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1t7h" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Homo sapiens~~]]	+	[[Category: Human]]
	[[Category: Aumelas, A]]		[[Category: Aumelas, A]]
	[[Category: Cerdan, R]]		[[Category: Cerdan, R]]

OCA at 22:42, 25 December 2014

OCA — Thu, 25 Dec 2014 22:42:44 GMT

←Older revision		Revision as of 22:42, 25 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t7h RCSB], [http://www.ebi.ac.uk/pdbsum/1t7h PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t7h RCSB], [http://www.ebi.ac.uk/pdbsum/1t7h PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/EDN1_HUMAN EDN1_HUMAN]] Endothelins are endothelium-derived vasoconstrictor peptides.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Aumelas, A.]]	+	[[Category: Aumelas, A]]
-	[[Category: Cerdan, R.]]	+	[[Category: Cerdan, R]]
-	[[Category: Chiche, L.]]	+	[[Category: Chiche, L]]
-	[[Category: Chino, N.]]	+	[[Category: Chino, N]]
-	[[Category: Dumas, C.]]	+	[[Category: Dumas, C]]
-	[[Category: Hoh, F.]]	+	[[Category: Hoh, F]]
-	[[Category: Kaas, Q.]]	+	[[Category: Kaas, Q]]
-	[[Category: Kobayashi, Y.]]	+	[[Category: Kobayashi, Y]]
-	[[Category: Kubo, S.]]	+	[[Category: Kubo, S]]
-	[[Category: Nishi, Y.]]	+	[[Category: Nishi, Y]]
	[[Category: Cysteine stabilized helical motif]]		[[Category: Cysteine stabilized helical motif]]
	[[Category: Dimer]]		[[Category: Dimer]]

OCA at 08:19, 8 October 2014

OCA — Wed, 08 Oct 2014 08:19:47 GMT

←Older revision		Revision as of 08:19, 8 October 2014
Line 1:		Line 1:
-	[[~~Image:~~1t7h.~~png~~\|~~left\|200px~~]]	+	==X-ray structure of [Lys(-2)-Arg(-1)-des(17-21)]-endothelin-1 peptide==
		+	<StructureSection load='1t7h' size='340' side='right' caption='[[1t7h]], [[Resolution\|resolution]] 1.13Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1t7h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7H OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T7H FirstGlance]. <br>
		+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">EDN1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t7h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t7h OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t7h RCSB], [http://www.ebi.ac.uk/pdbsum/1t7h PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Previous structural studies on the [Lys((-2))-Arg((-1))]endothelin-1 peptide (KR-ET-1), 540-fold less potent than ET-1, strongly suggested the presence of an intramolecular Arg(-1)-Asp(8) (R(-1)-D(8)) salt bridge that was also observed in the shorter [Lys((-2))-Arg((-1))-des(17-21)]endothelin-1 derivative (KR-CSH-ET). In addition, for these two analogues, we have shown that the Lys-Arg dipeptide, which belongs to the prosequence, significantly improves the formation of the native disulfide bonds (>or=96% instead of approximately 70% for ET-1). In contrast to what was inferred from NMR data, molecular dynamics simulations suggested that such an intramolecular salt bridge would be unstable. The KR-CSH-ET peptide has now been crystallized at pH 5.0 and its high-resolution structure determined ab initio at 1.13 A using direct methods. Unexpectedly, KR-CSH-ET was shown to be a head-to-tail symmetric dimer, and the overall interface involves two intermolecular R(-1)-D(8) salt bridges, a two-stranded antiparallel beta-sheet, and hydrophobic contacts. Molecular dynamics simulations carried out on this dimer clearly showed that the two intermolecular salt bridges were in this case very stable. Sedimentation equilibrium experiments unambiguously confirmed that KR-ET-1 and KR-CSH-ET also exist as dimers in solution at pH 5.0. On the basis of the new dimeric structure, previous NMR data were reinterpreted. Structure calculations were performed using 484 intramolecular and 38 intermolecular NMR-derived constraints. The solution and the X-ray structures of the dimer are very similar (mean rmsd of 0.85 A). Since the KR dipeptide at the N-terminus of KR-CSH-ET is present in the prosequence, it can be hypothesized that similar intermolecular salt bridges could be involved in the in vivo formation of the native disulfide bonds of ET-1. Therefore, it appears to be likely that the prosequence does assist the ET-1 folding in a chaperone-like manner before successive cleavages that yield the bioactive ET-1 hormone.

-	~~{{STRUCTURE_1t7h\| PDB=1t7h \| SCENE= }}~~	+	High-resolution X-ray structure of the unexpectedly stable dimer of the [Lys(-2)-Arg(-1)-des(17-21)]endothelin-1 peptide.,Hoh F, Cerdan R, Kaas Q, Nishi Y, Chiche L, Kubo S, Chino N, Kobayashi Y, Dumas C, Aumelas A Biochemistry. 2004 Dec 7;43(48):15154-68. PMID:15568807<ref>PMID:15568807</ref>

-	~~===X-ray structure~~ of ~~[Lys(-2)-Arg(-1)-des(17-21)]-endothelin-1 peptide===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15568807}}~~	+	== References ==
-		+	<references/>
-	==~~About this Structure~~==	+	__TOC__
-	~~[[1t7h]] is a 2 chain structure with sequence from [http:~~//~~en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7H OCA].~~	+	</StructureSection>
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
	[[Category: Aumelas, A.]]		[[Category: Aumelas, A.]]

OCA: Protected "1t7h" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 13:37:19 GMT

Protected "1t7h" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:37, 5 January 2013

OCA at 13:37, 5 January 2013

OCA — Sat, 05 Jan 2013 13:37:16 GMT

←Older revision		Revision as of 13:37, 5 January 2013
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-	{{Seed}}
	[[Image:1t7h.png\|left\|200px]]		[[Image:1t7h.png\|left\|200px]]

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	{{STRUCTURE_1t7h\| PDB=1t7h \| SCENE= }}		{{STRUCTURE_1t7h\| PDB=1t7h \| SCENE= }}

	===X-ray structure of [Lys(-2)-Arg(-1)-des(17-21)]-endothelin-1 peptide===		===X-ray structure of [Lys(-2)-Arg(-1)-des(17-21)]-endothelin-1 peptide===

-
-	<!--
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	{{ABSTRACT_PUBMED_15568807}}		{{ABSTRACT_PUBMED_15568807}}
-
-	==Disease==
-	Known disease associated with this structure: High density lipoprotein cholesterol level QTL 7 OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=131240 131240]]

	==About this Structure==		==About this Structure==
-	~~1T7H is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7H OCA].	+	[[1t7h]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T7H OCA].
-		+
-	~~==Reference==~~	+
-	High-resolution X-ray structure of the unexpectedly stable dimer of the [Lys(-2)-Arg(-1)-des(17-21)]endothelin-1 peptide., Hoh F, Cerdan R, Kaas Q, Nishi Y, Chiche L, Kubo S, Chino N, Kobayashi Y, Dumas C, Aumelas A, Biochemistry. 2004 Dec 7;43(48):15154-68. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15568807 15568807]	+
	[[Category: Homo sapiens]]		[[Category: Homo sapiens]]
-	[[Category: Single protein]]
	[[Category: Aumelas, A.]]		[[Category: Aumelas, A.]]
	[[Category: Cerdan, R.]]		[[Category: Cerdan, R.]]
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	[[Category: Endothelin]]		[[Category: Endothelin]]
	[[Category: Salt bridge]]		[[Category: Salt bridge]]
-		+	[[Category: Signaling protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Jul 29 16:24:05 2008''~~	+

OCA at 13:24, 29 July 2008

OCA — Tue, 29 Jul 2008 13:24:12 GMT

←Older revision		Revision as of 13:24, 29 July 2008
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	<!--		<!--
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	{{STRUCTURE_1t7h\| PDB=1t7h \| SCENE= }}		{{STRUCTURE_1t7h\| PDB=1t7h \| SCENE= }}

-	~~'''~~X-ray structure of [Lys(-2)-Arg(-1)-des(17-21)]-endothelin-1 peptide~~'''~~	+	===X-ray structure of [Lys(-2)-Arg(-1)-des(17-21)]-endothelin-1 peptide===


-	~~==Overview==~~	+	<!--
-	~~Previous structural studies on the [Lys((~~-~~2))~~-~~Arg((-1))]endothelin-1 peptide (KR-ET-1)~~, ~~540-fold less potent than ET-1~~, ~~strongly suggested~~ the presence of an intramolecular Arg(-1)-Asp(8) (R(-1)-D(8)) salt bridge that was also observed in the shorter [Lys((-2))-Arg((-1))-des(17-21)]endothelin-1 derivative (KR-CSH-ET). In addition, for these two analogues, we have shown that the Lys-Arg dipeptide, which belongs to the ~~prosequence, significantly improves the formation of the native disulfide bonds~~ (>or=96% instead of approximately 70% for ET-1). In contrast to what was inferred from NMR data, molecular dynamics simulations suggested that such an intramolecular salt bridge would be unstable. The KR-CSH-ET peptide has now been crystallized at ~~pH 5~~.~~0 and its high-resolution structure determined ab initio at 1~~.~~13 A using direct methods. Unexpectedly, KR-CSH-ET was shown to be a head-to-tail symmetric dimer, and the overall interface involves two intermolecular R(-1)-D(8~~) ~~salt bridges~~, a two-stranded antiparallel beta-sheet, and hydrophobic contacts. Molecular dynamics simulations carried out on this dimer clearly showed that the two intermolecular salt bridges were in this case very stable. Sedimentation equilibrium experiments unambiguously confirmed that KR-ET-1 and KR-CSH-ET also exist as dimers in solution at pH 5.0. On the basis of the new dimeric structure, previous NMR data were reinterpreted. Structure calculations were performed using 484 intramolecular and 38 intermolecular NMR-derived constraints. The solution and the X-ray structures of the dimer are very similar (mean rmsd of 0.85 A). Since the KR dipeptide at the N-terminus of KR-CSH-ET is ~~present in~~ the ~~prosequence, it can be hypothesized that similar intermolecular salt bridges could be involved in the in vivo formation of the native disulfide bonds of ET-1~~. ~~Therefore, it appears to be likely that the prosequence does assist the ET~~-~~1 folding in a chaperone~~-~~like manner before successive cleavages that yield the bioactive ET-1 hormone.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_15568807}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15568807 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15568807}}

	==Disease==		==Disease==
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	[[Category: Endothelin]]		[[Category: Endothelin]]
	[[Category: Salt bridge]]		[[Category: Salt bridge]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 09~~:37:45 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 16:24:05 2008''

OCA at 06:37, 3 May 2008

OCA — Sat, 03 May 2008 06:37:46 GMT

←Older revision		Revision as of 06:37, 3 May 2008
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-	~~{{Structure~~	+	<!--
-	~~\|PDB= 1t7h \|SIZE=350\|CAPTION= <scene name='initialview01'>1t7h</scene>~~, ~~resolution 1~~.~~13Å~~	+	The line below this paragraph, containing "STRUCTURE_1t7h", creates the "Structure Box" on the page.
-	~~\|SITE=~~	+	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	~~\|LIGAND=~~	+	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	~~\|ACTIVITY=~~	+	or leave the SCENE parameter empty for the default display.
-	~~\|GENE= EDN1~~ (~~[http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]~~)	+	-->
-	~~\|DOMAIN=~~	+	{{STRUCTURE_1t7h\| PDB=1t7h \| SCENE= }}
-	~~\|RELATEDENTRY=~~	+
-	\|~~RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol~~=1t7h ~~FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id~~=~~1t7h OCA], [http://www.ebi.ac.uk/pdbsum/1t7h PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1t7h RCSB]</span>~~	+
-	}}	+

	'''X-ray structure of [Lys(-2)-Arg(-1)-des(17-21)]-endothelin-1 peptide'''		'''X-ray structure of [Lys(-2)-Arg(-1)-des(17-21)]-endothelin-1 peptide'''
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	[[Category: Kubo, S.]]		[[Category: Kubo, S.]]
	[[Category: Nishi, Y.]]		[[Category: Nishi, Y.]]
-	[[Category: ~~cysteine~~ stabilized helical motif]]	+	[[Category: Cysteine stabilized helical motif]]
-	[[Category: ~~dimer~~]]	+	[[Category: Dimer]]
-	[[Category: ~~endothelin~~]]	+	[[Category: Endothelin]]
-	[[Category: ~~salt~~ bridge]]	+	[[Category: Salt bridge]]
-		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 09:37:45 2008''
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sun Mar 30 23~~:52:22 2008''	+