1t8k - Revision history

OCA at 08:37, 14 February 2024

OCA — Wed, 14 Feb 2024 08:37:32 GMT

←Older revision		Revision as of 08:37, 14 February 2024
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	<StructureSection load='1t8k' size='340' side='right'caption='[[1t8k]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='1t8k' size='340' side='right'caption='[[1t8k]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T8K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T8K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [https://pdbe.org/1t8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB], [https://www.ebi.ac.uk/pdbsum/1t8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8k ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [https://pdbe.org/1t8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB], [https://www.ebi.ac.uk/pdbsum/1t8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ACP_ECOLI ACP_ECOLI]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217]	+	[https://www.uniprot.org/uniprot/ACP_ECOLI ACP_ECOLI] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t8k ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t8k ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	A topic of current interest is engineering surface mutations in order to improve the success rate of protein crystallization. This report explores the possibility of using metal-ion-mediated crystal-packing interactions to facilitate rational design. Escherichia coli apo acyl carrier protein was chosen as a test case because of its high content of negatively charged carboxylates suitable for metal binding with moderate affinity. The protein was successfully crystallized in the presence of zinc ions. The crystal structure was determined to 1.1 A resolution with MAD phasing using anomalous signals from the co-crystallized Zn(2+) ions. The case study suggested an integrated strategy for crystallization and structure solution of proteins via engineering surface Asp and Glu mutants, crystallizing them in the presence of metal ions such as Zn(2+) and solving the structures using anomalous signals.
-
-	Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions.,Qiu X, Janson CA Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1545-54. Epub 2004, Aug 26. PMID:15333924<ref>PMID:15333924</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1t8k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Acyl carrier protein 3D structures\|Acyl carrier protein 3D structures]]		*[[Acyl carrier protein 3D structures\|Acyl carrier protein 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Janson~~, C A~~]]	+	[[Category: Janson CA]]
-	[[Category: Qiu, X]]	+	[[Category: Qiu X]]
-	~~[[Category: Acp]]~~	+
-	~~[[Category: Lipid transport~~]]	+

OCA at 09:23, 29 September 2021

OCA — Wed, 29 Sep 2021 09:23:59 GMT

←Older revision		Revision as of 09:23, 29 September 2021
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	<StructureSection load='1t8k' size='340' side='right'caption='[[1t8k]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='1t8k' size='340' side='right'caption='[[1t8k]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1T8K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T8K FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [~~http~~://pdbe.org/1t8k PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1t8k PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8k ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [https://pdbe.org/1t8k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB], [https://www.ebi.ac.uk/pdbsum/1t8k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ACP_ECOLI ACP_ECOLI]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217]	+	[[https://www.uniprot.org/uniprot/ACP_ECOLI ACP_ECOLI]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	==See Also==		==See Also==
	*[[Acyl carrier protein 3D structures\|Acyl carrier protein 3D structures]]		*[[Acyl carrier protein 3D structures\|Acyl carrier protein 3D structures]]
-	*[[Fatty acid synthase 3D structures\|Fatty acid synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:08, 18 December 2019

OCA — Wed, 18 Dec 2019 14:08:48 GMT

←Older revision		Revision as of 14:08, 18 December 2019
Line 1:		Line 1:

	==Crystal Structure of apo acyl carrier protein from E. coli==		==Crystal Structure of apo acyl carrier protein from E. coli==
-	<StructureSection load='1t8k' size='340' side='right' caption='[[1t8k]], [[Resolution\|resolution]] 1.10Å' scene=''>	+	<StructureSection load='1t8k' size='340' side='right'caption='[[1t8k]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8K FirstGlance]. <br>		<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8K FirstGlance]. <br>
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Acyl carrier protein\|Acyl carrier protein]]	+	*[[Acyl carrier protein 3D structures\|Acyl carrier protein 3D structures]]
-	*[[Fatty acid synthase\|Fatty acid synthase]]	+	*[[Fatty acid synthase 3D structures\|Fatty acid synthase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 37:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Janson, C A]]		[[Category: Janson, C A]]
	[[Category: Qiu, X]]		[[Category: Qiu, X]]
	[[Category: Acp]]		[[Category: Acp]]
	[[Category: Lipid transport]]		[[Category: Lipid transport]]

OCA at 07:49, 14 March 2018

OCA — Wed, 14 Mar 2018 07:49:51 GMT

←Older revision		Revision as of 07:49, 14 March 2018
Line 1:		Line 1:
		+
	==Crystal Structure of apo acyl carrier protein from E. coli==		==Crystal Structure of apo acyl carrier protein from E. coli==
	<StructureSection load='1t8k' size='340' side='right' caption='[[1t8k]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='1t8k' size='340' side='right' caption='[[1t8k]], [[Resolution\|resolution]] 1.10Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8K FirstGlance]. <br>		<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [http://pdbe.org/1t8k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB], [http://www.ebi.ac.uk/pdbsum/1t8k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [http://pdbe.org/1t8k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB], [http://www.ebi.ac.uk/pdbsum/1t8k PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8k ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t8/1t8k_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t8/1t8k_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t8k ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:50, 12 September 2015

OCA — Sat, 12 Sep 2015 00:50:09 GMT

←Older revision		Revision as of 00:50, 12 September 2015
Line 2:		Line 2:
	<StructureSection load='1t8k' size='340' side='right' caption='[[1t8k]], [[Resolution\|resolution]] 1.10Å' scene=''>		<StructureSection load='1t8k' size='340' side='right' caption='[[1t8k]], [[Resolution\|resolution]] 1.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8K FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8K FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB], [http://www.ebi.ac.uk/pdbsum/1t8k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [http://pdbe.org/1t8k PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB], [http://www.ebi.ac.uk/pdbsum/1t8k PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1t8k" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 34:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Janson, C A]]		[[Category: Janson, C A]]
	[[Category: Qiu, X]]		[[Category: Qiu, X]]
	[[Category: Acp]]		[[Category: Acp]]
	[[Category: Lipid transport]]		[[Category: Lipid transport]]

OCA at 21:37, 25 December 2014

OCA — Thu, 25 Dec 2014 21:37:34 GMT

←Older revision		Revision as of 21:37, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8K FirstGlance]. <br>		<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8K FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB], [http://www.ebi.ac.uk/pdbsum/1t8k PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB], [http://www.ebi.ac.uk/pdbsum/1t8k PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACP_ECOLI ACP_ECOLI]] Carrier of the growing fatty acid chain in fatty acid biosynthesis.[HAMAP-Rule:MF_01217]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Janson, C A.]]	+	[[Category: Janson, C A]]
-	[[Category: Qiu, X.]]	+	[[Category: Qiu, X]]
	[[Category: Acp]]		[[Category: Acp]]
	[[Category: Lipid transport]]		[[Category: Lipid transport]]

OCA at 22:55, 28 September 2014

OCA — Sun, 28 Sep 2014 22:55:58 GMT

←Older revision		Revision as of 22:55, 28 September 2014
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-	[[~~Image~~:1t8k.~~png\|left~~\|200px]]	+	==Crystal Structure of apo acyl carrier protein from E. coli==
		+	<StructureSection load='1t8k' size='340' side='right' caption='[[1t8k]], [[Resolution\|resolution]] 1.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1t8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8K FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8k OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t8k RCSB], [http://www.ebi.ac.uk/pdbsum/1t8k PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t8/1t8k_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	A topic of current interest is engineering surface mutations in order to improve the success rate of protein crystallization. This report explores the possibility of using metal-ion-mediated crystal-packing interactions to facilitate rational design. Escherichia coli apo acyl carrier protein was chosen as a test case because of its high content of negatively charged carboxylates suitable for metal binding with moderate affinity. The protein was successfully crystallized in the presence of zinc ions. The crystal structure was determined to 1.1 A resolution with MAD phasing using anomalous signals from the co-crystallized Zn(2+) ions. The case study suggested an integrated strategy for crystallization and structure solution of proteins via engineering surface Asp and Glu mutants, crystallizing them in the presence of metal ions such as Zn(2+) and solving the structures using anomalous signals.

-	~~{{STRUCTURE_1t8k\| PDB=1t8k \| SCENE= }}~~	+	Structure of apo acyl carrier protein and a proposal to engineer protein crystallization through metal ions.,Qiu X, Janson CA Acta Crystallogr D Biol Crystallogr. 2004 Sep;60(Pt 9):1545-54. Epub 2004, Aug 26. PMID:15333924<ref>PMID:15333924</ref>

-	~~===Crystal Structure~~ of ~~apo acyl carrier protein from E~~. ~~coli===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15333924}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1t8k]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA].~~	+

	==See Also==		==See Also==
		+	*[[Acyl carrier protein\|Acyl carrier protein]]
	*[[Fatty acid synthase\|Fatty acid synthase]]		*[[Fatty acid synthase\|Fatty acid synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015333924<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Janson, C A.]]		[[Category: Janson, C A.]]

OCA at 21:57, 20 October 2012

OCA — Sat, 20 Oct 2012 21:57:46 GMT

←Older revision		Revision as of 21:57, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1t8k]] is a 1 chain structure ~~of [[Fatty acid synthase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA].	+	[[1t8k]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA].

	==See Also==		==See Also==

OCA: Protected "1t8k" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 14:46:42 GMT

Protected "1t8k" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:46, 27 July 2012

OCA at 14:46, 27 July 2012

OCA — Fri, 27 Jul 2012 14:46:40 GMT

←Older revision		Revision as of 14:46, 27 July 2012
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-	{{Seed}}
	[[Image:1t8k.png\|left\|200px]]		[[Image:1t8k.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1t8k", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1t8k\| PDB=1t8k \| SCENE= }}		{{STRUCTURE_1t8k\| PDB=1t8k \| SCENE= }}

	===Crystal Structure of apo acyl carrier protein from E. coli===		===Crystal Structure of apo acyl carrier protein from E. coli===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15333924}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15333924 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_15333924}}		{{ABSTRACT_PUBMED_15333924}}

	==About this Structure==		==About this Structure==
-	~~1T8K~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA].	+	[[1t8k]] is a 1 chain structure of [[Fatty acid synthase]] with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8K OCA].
		+
		+	==See Also==
		+	*[[Fatty acid synthase\|Fatty acid synthase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15333924~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015333924</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Janson, C A.]]		[[Category: Janson, C A.]]
	[[Category: Qiu, X.]]		[[Category: Qiu, X.]]
	[[Category: Acp]]		[[Category: Acp]]
-		+	[[Category: Lipid transport]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 08:39:02 2009''~~	+