1t8m - Revision history

OCA at 06:27, 23 August 2023

OCA — Wed, 23 Aug 2023 06:27:37 GMT

←Older revision		Revision as of 06:27, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1t8m' size='340' side='right'caption='[[1t8m]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1t8m' size='340' side='right'caption='[[1t8m]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t8m]] is a 4 chain structure with sequence from ~~[https://en.wikipedia.org/wiki/Bovin Bovin] and~~ [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T8M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t8m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T8M FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1t7c\|1t7c]], [[1t8l\|1t8l]], [[1t8n\|1t8n]], [[1t8o\|1t8o]], [[1p2m\|1p2m]], [[1p2o\|1p2o]], [[1p2n\|1p2n]], [[1p2q\|1p2q]], [[1cbw\|1cbw]], [[1mtn\|1mtn]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8m OCA], [https://pdbe.org/1t8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t8m RCSB], [https://www.ebi.ac.uk/pdbsum/1t8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8m ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8m OCA], [https://pdbe.org/1t8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t8m RCSB], [https://www.ebi.ac.uk/pdbsum/1t8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~BPT1_BOVIN BPT1_BOVIN~~]~~] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.~~	+	[https://www.uniprot.org/uniprot/CTRA_BOVIN CTRA_BOVIN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 39:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
-	[[Category: Bovin]]
-	[[Category: Chymotrypsin]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Czapinska, H]]	+	[[Category: Czapinska H]]
-	[[Category: Helland, R]]	+	[[Category: Helland R]]
-	[[Category: Otlewski, J]]	+	[[Category: Otlewski J]]
-	[[Category: Smalas~~, A O]]~~	+	[[Category: Smalas AO]]
-	~~[[Category: Bovine pancreatic trypsin inhibitor]]~~	+
-	~~[[Category: Bpti]]~~	+
-	~~[[Category: Hydrolase-hydrolase inhibitor complex]]~~	+
-	~~[[Category: Non-cognate binding]]~~	+
-	~~[[Category: Primary specificity]]~~	+
-	~~[[Category: Protein-protein interaction]]~~	+
-	~~[[Category: S1 pocket]]~~	+
-	~~[[Category: Serine proteinase~~]]	+

OCA at 09:24, 29 September 2021

OCA — Wed, 29 Sep 2021 09:24:04 GMT

←Older revision		Revision as of 09:24, 29 September 2021
Line 3:		Line 3:
	<StructureSection load='1t8m' size='340' side='right'caption='[[1t8m]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1t8m' size='340' side='right'caption='[[1t8m]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t8m]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bovin Bovin] and [~~http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1T8M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t8m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin] and [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1T8M FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t7c\|1t7c]], [[1t8l\|1t8l]], [[1t8n\|1t8n]], [[1t8o\|1t8o]], [[1p2m\|1p2m]], [[1p2o\|1p2o]], [[1p2n\|1p2n]], [[1p2q\|1p2q]], [[1cbw\|1cbw]], [[1mtn\|1mtn]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1t7c\|1t7c]], [[1t8l\|1t8l]], [[1t8n\|1t8n]], [[1t8o\|1t8o]], [[1p2m\|1p2m]], [[1p2o\|1p2o]], [[1p2n\|1p2n]], [[1p2q\|1p2q]], [[1cbw\|1cbw]], [[1mtn\|1mtn]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1t8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8m OCA], [~~http~~://pdbe.org/1t8m PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1t8m RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1t8m PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8m ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1t8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8m OCA], [https://pdbe.org/1t8m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1t8m RCSB], [https://www.ebi.ac.uk/pdbsum/1t8m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN]] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.	+	[[https://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN]] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 14:14, 18 December 2019

OCA — Wed, 18 Dec 2019 14:14:01 GMT

←Older revision		Revision as of 14:14, 18 December 2019
Line 1:		Line 1:

	==CRYSTAL STRUCTURE OF THE P1 HIS BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX==		==CRYSTAL STRUCTURE OF THE P1 HIS BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX==
-	<StructureSection load='1t8m' size='340' side='right' caption='[[1t8m]], [[Resolution\|resolution]] 1.80Å' scene=''>	+	<StructureSection load='1t8m' size='340' side='right'caption='[[1t8m]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1t8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8M FirstGlance]. <br>		<table><tr><td colspan='2'>[[1t8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8M FirstGlance]. <br>
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[~~Basic Pancreatic Trypsin Inhibitor~~\|~~Basic Pancreatic Trypsin Inhibitor~~]]	+	*[[BPTI 3D structures\|BPTI 3D structures]]
-	*[[Chymotrypsin\|Chymotrypsin]]	+	*[[Chymotrypsin 3D structures\|Chymotrypsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 41:		Line 41:
	[[Category: Bovin]]		[[Category: Bovin]]
	[[Category: Chymotrypsin]]		[[Category: Chymotrypsin]]
		+	[[Category: Large Structures]]
	[[Category: Czapinska, H]]		[[Category: Czapinska, H]]
	[[Category: Helland, R]]		[[Category: Helland, R]]

OCA at 07:54, 14 March 2018

OCA — Wed, 14 Mar 2018 07:54:00 GMT

←Older revision		Revision as of 07:54, 14 March 2018
Line 1:		Line 1:
		+
	==CRYSTAL STRUCTURE OF THE P1 HIS BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX==		==CRYSTAL STRUCTURE OF THE P1 HIS BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX==
	<StructureSection load='1t8m' size='340' side='right' caption='[[1t8m]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1t8m' size='340' side='right' caption='[[1t8m]], [[Resolution\|resolution]] 1.80Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t7c\|1t7c]], [[1t8l\|1t8l]], [[1t8n\|1t8n]], [[1t8o\|1t8o]], [[1p2m\|1p2m]], [[1p2o\|1p2o]], [[1p2n\|1p2n]], [[1p2q\|1p2q]], [[1cbw\|1cbw]], [[1mtn\|1mtn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t7c\|1t7c]], [[1t8l\|1t8l]], [[1t8n\|1t8n]], [[1t8o\|1t8o]], [[1p2m\|1p2m]], [[1p2o\|1p2o]], [[1p2n\|1p2n]], [[1p2q\|1p2q]], [[1cbw\|1cbw]], [[1mtn\|1mtn]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8m OCA], [http://pdbe.org/1t8m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t8m RCSB], [http://www.ebi.ac.uk/pdbsum/1t8m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8m OCA], [http://pdbe.org/1t8m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t8m RCSB], [http://www.ebi.ac.uk/pdbsum/1t8m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1t8m ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t8/1t8m_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t8/1t8m_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 03:26, 10 February 2016

OCA — Wed, 10 Feb 2016 03:26:52 GMT

←Older revision		Revision as of 03:26, 10 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1t8m ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:54, 11 September 2015

OCA — Fri, 11 Sep 2015 04:54:54 GMT

←Older revision		Revision as of 04:54, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1t8m' size='340' side='right' caption='[[1t8m]], [[Resolution\|resolution]] 1.80Å' scene=''>		<StructureSection load='1t8m' size='340' side='right' caption='[[1t8m]], [[Resolution\|resolution]] 1.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1t8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8M FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1t8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin] and [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8M FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t7c\|1t7c]], [[1t8l\|1t8l]], [[1t8n\|1t8n]], [[1t8o\|1t8o]], [[1p2m\|1p2m]], [[1p2o\|1p2o]], [[1p2n\|1p2n]], [[1p2q\|1p2q]], [[1cbw\|1cbw]], [[1mtn\|1mtn]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t7c\|1t7c]], [[1t8l\|1t8l]], [[1t8n\|1t8n]], [[1t8o\|1t8o]], [[1p2m\|1p2m]], [[1p2o\|1p2o]], [[1p2n\|1p2n]], [[1p2q\|1p2q]], [[1cbw\|1cbw]], [[1mtn\|1mtn]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t8m RCSB], [http://www.ebi.ac.uk/pdbsum/1t8m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8m OCA], [http://pdbe.org/1t8m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1t8m RCSB], [http://www.ebi.ac.uk/pdbsum/1t8m PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1t8m" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
		+	[[Category: Bovin]]
	[[Category: Chymotrypsin]]		[[Category: Chymotrypsin]]
	[[Category: Czapinska, H]]		[[Category: Czapinska, H]]

OCA at 11:28, 25 December 2014

OCA — Thu, 25 Dec 2014 11:28:18 GMT

←Older revision		Revision as of 11:28, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1t8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8M FirstGlance]. <br>		<table><tr><td colspan='2'>[[1t8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8M FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t7c\|1t7c]], [[1t8l\|1t8l]], [[1t8n\|1t8n]], [[1t8o\|1t8o]], [[1p2m\|1p2m]], [[1p2o\|1p2o]], [[1p2n\|1p2n]], [[1p2q\|1p2q]], [[1cbw\|1cbw]], [[1mtn\|1mtn]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t7c\|1t7c]], [[1t8l\|1t8l]], [[1t8n\|1t8n]], [[1t8o\|1t8o]], [[1p2m\|1p2m]], [[1p2o\|1p2o]], [[1p2n\|1p2n]], [[1p2q\|1p2q]], [[1cbw\|1cbw]], [[1mtn\|1mtn]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t8m RCSB], [http://www.ebi.ac.uk/pdbsum/1t8m PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t8m RCSB], [http://www.ebi.ac.uk/pdbsum/1t8m PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BPT1_BOVIN BPT1_BOVIN]] Inhibits trypsin, kallikrein, chymotrypsin, and plasmin.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Chymotrypsin]]		[[Category: Chymotrypsin]]
-	[[Category: Czapinska, H.]]	+	[[Category: Czapinska, H]]
-	[[Category: Helland, R.]]	+	[[Category: Helland, R]]
-	[[Category: Otlewski, J.]]	+	[[Category: Otlewski, J]]
-	[[Category: Smalas, A O.]]	+	[[Category: Smalas, A O]]
	[[Category: Bovine pancreatic trypsin inhibitor]]		[[Category: Bovine pancreatic trypsin inhibitor]]
	[[Category: Bpti]]		[[Category: Bpti]]
-	[[Category: Chymotrypsin]]
	[[Category: Hydrolase-hydrolase inhibitor complex]]		[[Category: Hydrolase-hydrolase inhibitor complex]]
	[[Category: Non-cognate binding]]		[[Category: Non-cognate binding]]

OCA at 19:45, 28 September 2014

OCA — Sun, 28 Sep 2014 19:45:43 GMT

←Older revision		Revision as of 19:45, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1t8m.~~png~~\|~~left~~\|200px]]	+	==CRYSTAL STRUCTURE OF THE P1 HIS BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX==
		+	<StructureSection load='1t8m' size='340' side='right' caption='[[1t8m]], [[Resolution\|resolution]] 1.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1t8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1T8M FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1t7c\|1t7c]], [[1t8l\|1t8l]], [[1t8n\|1t8n]], [[1t8o\|1t8o]], [[1p2m\|1p2m]], [[1p2o\|1p2o]], [[1p2n\|1p2n]], [[1p2q\|1p2q]], [[1cbw\|1cbw]], [[1mtn\|1mtn]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Chymotrypsin Chymotrypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.1 3.4.21.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1t8m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1t8m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1t8m RCSB], [http://www.ebi.ac.uk/pdbsum/1t8m PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/t8/1t8m_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The bovine chymotrypsin-bovine pancreatic trypsin inhibitor (BPTI) interaction belongs to extensively studied models of protein-protein recognition. The accommodation of the inhibitor P1 residue in the S1 binding site of the enzyme forms the hot spot of this interaction. Mutations introduced at the P1 position of BPTI result in a more than five orders of magnitude difference of the association constant values with the protease. To elucidate the structural aspects of the discrimination between different P1 residues, crystal structures of five bovine chymotrypsin-P1 BPTI variant complexes have been determined at pH 7.8 to a resolution below 2 A. The set includes polar (Thr), ionizable (Glu, His), medium-sized aliphatic (Met) and large aromatic (Trp) P1 residues and complements our earlier studies of the interaction of different P1 side-chains with the S1 pocket of chymotrypsin. The structures have been compared to the complexes of proteases with similar and dissimilar P1 preferences, including Streptomyces griseus proteases B and E, human neutrophil elastase, crab collagenase, bovine trypsin and human thrombin. The S1 sites of these enzymes share a common general shape of significant rigidity. Large and branched P1 residues adapt in their complexes similar conformations regardless of the polarity and size differences between their S1 pockets. Conversely, long and flexible residues such as P1 Met are present in the disordered form and display a conformational diversity despite similar inhibitory properties with respect to most enzymes studied. Thus, the S1 specificity profiles of the serine proteases appear to result from the precise complementarity of the P1-S1 interface and minor conformational adjustments occurring upon the inhibitor binding.

-	~~{{STRUCTURE_1t8m\| PDB=1t8m \| SCENE= }}~~	+	Crystal structures of five bovine chymotrypsin complexes with P1 BPTI variants.,Czapinska H, Helland R, Smalas AO, Otlewski J J Mol Biol. 2004 Dec 3;344(4):1005-20. PMID:15544809<ref>PMID:15544809</ref>

-	~~===CRYSTAL STRUCTURE OF THE P1 HIS BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15544809}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1t8m]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA].~~	+

	==See Also==		==See Also==
	*[[Basic Pancreatic Trypsin Inhibitor\|Basic Pancreatic Trypsin Inhibitor]]		*[[Basic Pancreatic Trypsin Inhibitor\|Basic Pancreatic Trypsin Inhibitor]]
	*[[Chymotrypsin\|Chymotrypsin]]		*[[Chymotrypsin\|Chymotrypsin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015544809<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Chymotrypsin]]		[[Category: Chymotrypsin]]

OCA at 16:10, 20 October 2012

OCA — Sat, 20 Oct 2012 16:10:16 GMT

←Older revision		Revision as of 16:10, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1t8m]] is a 4 chain structure ~~of [[Basic Pancreatic Trypsin Inhibitor]] and [[Chymotrypsin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA].	+	[[1t8m]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1T8M OCA].

	==See Also==		==See Also==

OCA at 20:49, 26 July 2012

OCA — Thu, 26 Jul 2012 20:49:38 GMT

←Older revision		Revision as of 20:49, 26 July 2012
Line 1:		Line 1:
	[[Image:1t8m.png\|left\|200px]]		[[Image:1t8m.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1t8m\| PDB=1t8m \| SCENE= }}		{{STRUCTURE_1t8m\| PDB=1t8m \| SCENE= }}

	===CRYSTAL STRUCTURE OF THE P1 HIS BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX===		===CRYSTAL STRUCTURE OF THE P1 HIS BPTI MUTANT- BOVINE CHYMOTRYPSIN COMPLEX===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15544809}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15544809 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_15544809}}		{{ABSTRACT_PUBMED_15544809}}

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	==See Also==		==See Also==
-	*[[Basic Pancreatic Trypsin Inhibitor]]	+	*[[Basic Pancreatic Trypsin Inhibitor\|Basic Pancreatic Trypsin Inhibitor]]
-	*[[Chymotrypsin]]	+	*[[Chymotrypsin\|Chymotrypsin]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15544809~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015544809</ref><references group="xtra"/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Chymotrypsin]]		[[Category: Chymotrypsin]]
Line 36:		Line 25:
	[[Category: Bpti]]		[[Category: Bpti]]
	[[Category: Chymotrypsin]]		[[Category: Chymotrypsin]]
-	~~[[Category: Crystal structure]]~~	+	[[Category: Hydrolase-hydrolase inhibitor complex]]
-	[[Category: Hydrolase/hydrolase inhibitor complex]]	+
	[[Category: Non-cognate binding]]		[[Category: Non-cognate binding]]
	[[Category: Primary specificity]]		[[Category: Primary specificity]]