1tca - Revision history

OCA at 08:39, 1 May 2024

OCA — Wed, 01 May 2024 08:39:56 GMT

←Older revision		Revision as of 08:39, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='1tca' size='340' side='right'caption='[[1tca]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='1tca' size='340' side='right'caption='[[1tca]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TCA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Moesziomyces_antarcticus Moesziomyces antarcticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TCA FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NAG:N~~-~~ACETYL-D-GLUCOSAMINE'>NAG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1tcb\|1tcb]], [[1tcc\|1tcc]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tca OCA], [https://pdbe.org/1tca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tca RCSB], [https://www.ebi.ac.uk/pdbsum/1tca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tca ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tca OCA], [https://pdbe.org/1tca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tca RCSB], [https://www.ebi.ac.uk/pdbsum/1tca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tca ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~LIPB_CANAR LIPB_CANAR]~~] Hydrolysis of triglycerides. Is very stereospecific both in hydrolysis and in organic synthesis and has a potentially important application in glucolipid synthesis.	+	[https://www.uniprot.org/uniprot/LIPB_PSEA2 LIPB_PSEA2] Hydrolysis of triglycerides. Is very stereospecific both in hydrolysis and in organic synthesis and has a potentially important application in glucolipid synthesis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tca ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tca ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	BACKGROUND: Lipases constitute a family of enzymes that hydrolyze triglycerides. They occur in many organisms and display a wide variety of substrate specificities. In recent years, much progress has been made towards explaining the mechanism of these enzymes and their ability to hydrolyze their substrates at an oil-water interface. RESULTS: We have determined the DNA and amino acid sequences for lipase B from the yeast Candida antarctica. The primary sequence has no significant homology to any other known lipase and deviates from the consensus sequence around the active site serine that is found in other lipases. We have determined the crystal structure of this enzyme using multiple isomorphous replacement methods for two crystal forms. Models for the orthorhombic and monoclinic crystal forms of the enzyme have been refined to 1.55 A and 2.1 A resolution, respectively. Lipase B is an alpha/beta type protein that has many features in common with previously determined lipase structures and other related enzymes. In the monoclinic crystal form, lipid-like molecules, most likely beta-octyl glucoside, can be seen close to the active site. The behaviour of these lipid molecules in the crystal structure has been studied at different pH values. CONCLUSION: The structure of Candida antarctica lipase B shows that the enzyme has a Ser-His-Asp catalytic triad in its active site. The structure appears to be in an 'open' conformation with a rather restricted entrance to the active site. We believe that this accounts for the substrate specificity and high degree of stereospecificity of this lipase.
-
-	The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica.,Uppenberg J, Hansen MT, Patkar S, Jones TA Structure. 1994 Apr 15;2(4):293-308. PMID:8087556<ref>PMID:8087556</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1tca" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Lipase 3D Structures\|Lipase 3D Structures]]		*[[Lipase 3D Structures\|Lipase 3D Structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Triacylglycerol lipase~~]]	+	[[Category: Moesziomyces antarcticus]]
-	[[Category: Jones~~, T A~~]]	+	[[Category: Jones TA]]
-	[[Category: Uppenberg, J]]	+	[[Category: Uppenberg J]]

OCA at 09:25, 29 September 2021

OCA — Wed, 29 Sep 2021 09:25:31 GMT

←Older revision		Revision as of 09:25, 29 September 2021
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	<StructureSection load='1tca' size='340' side='right'caption='[[1tca]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='1tca' size='340' side='right'caption='[[1tca]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1TCA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TCA FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tcb\|1tcb]], [[1tcc\|1tcc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1tcb\|1tcb]], [[1tcc\|1tcc]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1tca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tca OCA], [~~http~~://pdbe.org/1tca PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1tca RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1tca PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1tca ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tca OCA], [https://pdbe.org/1tca PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tca RCSB], [https://www.ebi.ac.uk/pdbsum/1tca PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tca ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/LIPB_CANAR LIPB_CANAR]] Hydrolysis of triglycerides. Is very stereospecific both in hydrolysis and in organic synthesis and has a potentially important application in glucolipid synthesis.	+	[[https://www.uniprot.org/uniprot/LIPB_CANAR LIPB_CANAR]] Hydrolysis of triglycerides. Is very stereospecific both in hydrolysis and in organic synthesis and has a potentially important application in glucolipid synthesis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:15, 4 December 2019

OCA — Wed, 04 Dec 2019 11:15:05 GMT

←Older revision		Revision as of 11:15, 4 December 2019
Line 1:		Line 1:

	==THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA==		==THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA==
-	<StructureSection load='1tca' size='340' side='right' caption='[[1tca]], [[Resolution\|resolution]] 1.55Å' scene=''>	+	<StructureSection load='1tca' size='340' side='right'caption='[[1tca]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TCA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TCA FirstGlance]. <br>
Line 15:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tc/1tca_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tc/1tca_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1tca" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1tca" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lipase 3D Structures\|Lipase 3D Structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Jones, T A]]		[[Category: Jones, T A]]
	[[Category: Uppenberg, J]]		[[Category: Uppenberg, J]]

OCA at 06:58, 29 November 2017

OCA — Wed, 29 Nov 2017 06:58:30 GMT

←Older revision		Revision as of 06:58, 29 November 2017
Line 1:		Line 1:
		+
	==THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA==		==THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA==
	<StructureSection load='1tca' size='340' side='right' caption='[[1tca]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='1tca' size='340' side='right' caption='[[1tca]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure ~~with sequence from [http://en.wikipedia.org/wiki/Canar Canar]~~. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TCA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TCA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tcb\|1tcb]], [[1tcc\|1tcc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tcb\|1tcb]], [[1tcc\|1tcc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tca OCA], [http://pdbe.org/1tca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tca RCSB], [http://www.ebi.ac.uk/pdbsum/1tca PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tca OCA], [http://pdbe.org/1tca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tca RCSB], [http://www.ebi.ac.uk/pdbsum/1tca PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tca ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tca ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1tca" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1tca" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Lipase\|Lipase]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Canar]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Jones, T A]]		[[Category: Jones, T A]]
	[[Category: Uppenberg, J]]		[[Category: Uppenberg, J]]

OCA at 14:22, 11 September 2015

OCA — Fri, 11 Sep 2015 14:22:44 GMT

←Older revision		Revision as of 14:22, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1tca' size='340' side='right' caption='[[1tca]], [[Resolution\|resolution]] 1.55Å' scene=''>		<StructureSection load='1tca' size='340' side='right' caption='[[1tca]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Candida_antarctica Candida antarctica~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TCA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Canar Canar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TCA FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tcb\|1tcb]], [[1tcc\|1tcc]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tcb\|1tcb]], [[1tcc\|1tcc]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tca OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tca RCSB], [http://www.ebi.ac.uk/pdbsum/1tca PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tca OCA], [http://pdbe.org/1tca PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tca RCSB], [http://www.ebi.ac.uk/pdbsum/1tca PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1tca" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Candida antarctica~~]]	+	[[Category: Canar]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Jones, T A]]		[[Category: Jones, T A]]
	[[Category: Uppenberg, J]]		[[Category: Uppenberg, J]]

OCA at 17:39, 25 December 2014

OCA — Thu, 25 Dec 2014 17:39:20 GMT

←Older revision		Revision as of 17:39, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_antarctica Candida antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TCA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_antarctica Candida antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TCA FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tcb\|1tcb]], [[1tcc\|1tcc]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tcb\|1tcb]], [[1tcc\|1tcc]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tca OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tca RCSB], [http://www.ebi.ac.uk/pdbsum/1tca PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tca OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tca RCSB], [http://www.ebi.ac.uk/pdbsum/1tca PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/LIPB_CANAR LIPB_CANAR]] Hydrolysis of triglycerides. Is very stereospecific both in hydrolysis and in organic synthesis and has a potentially important application in glucolipid synthesis.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Candida antarctica]]		[[Category: Candida antarctica]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
-	[[Category: Jones, T A.]]	+	[[Category: Jones, T A]]
-	[[Category: Uppenberg, J.]]	+	[[Category: Uppenberg, J]]

OCA at 22:43, 28 September 2014

OCA — Sun, 28 Sep 2014 22:43:55 GMT

←Older revision		Revision as of 22:43, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1tca.~~png~~\|~~left~~\|200px]]	+	==THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA==
		+	<StructureSection load='1tca' size='340' side='right' caption='[[1tca]], [[Resolution\|resolution]] 1.55Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1tca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_antarctica Candida antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TCA FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1tcb\|1tcb]], [[1tcc\|1tcc]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Triacylglycerol_lipase Triacylglycerol lipase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.3 3.1.1.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tca FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tca OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tca RCSB], [http://www.ebi.ac.uk/pdbsum/1tca PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tc/1tca_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	BACKGROUND: Lipases constitute a family of enzymes that hydrolyze triglycerides. They occur in many organisms and display a wide variety of substrate specificities. In recent years, much progress has been made towards explaining the mechanism of these enzymes and their ability to hydrolyze their substrates at an oil-water interface. RESULTS: We have determined the DNA and amino acid sequences for lipase B from the yeast Candida antarctica. The primary sequence has no significant homology to any other known lipase and deviates from the consensus sequence around the active site serine that is found in other lipases. We have determined the crystal structure of this enzyme using multiple isomorphous replacement methods for two crystal forms. Models for the orthorhombic and monoclinic crystal forms of the enzyme have been refined to 1.55 A and 2.1 A resolution, respectively. Lipase B is an alpha/beta type protein that has many features in common with previously determined lipase structures and other related enzymes. In the monoclinic crystal form, lipid-like molecules, most likely beta-octyl glucoside, can be seen close to the active site. The behaviour of these lipid molecules in the crystal structure has been studied at different pH values. CONCLUSION: The structure of Candida antarctica lipase B shows that the enzyme has a Ser-His-Asp catalytic triad in its active site. The structure appears to be in an 'open' conformation with a rather restricted entrance to the active site. We believe that this accounts for the substrate specificity and high degree of stereospecificity of this lipase.

-	~~{{STRUCTURE_1tca\| PDB=1tca \| SCENE= }}~~	+	The sequence, crystal structure determination and refinement of two crystal forms of lipase B from Candida antarctica.,Uppenberg J, Hansen MT, Patkar S, Jones TA Structure. 1994 Apr 15;2(4):293-308. PMID:8087556<ref>PMID:8087556</ref>

-	~~===THE SEQUENCE~~, ~~CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_8087556}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1tca]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Candida_antarctica Candida antarctica]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA].~~	+

	==See Also==		==See Also==
	*[[Lipase\|Lipase]]		*[[Lipase\|Lipase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:008087556<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Candida antarctica]]		[[Category: Candida antarctica]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Jones, T A.]]		[[Category: Jones, T A.]]
	[[Category: Uppenberg, J.]]		[[Category: Uppenberg, J.]]

OCA at 19:33, 20 October 2012

OCA — Sat, 20 Oct 2012 19:33:30 GMT

←Older revision		Revision as of 19:33, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1tca]] is a 1 chain structure ~~of [[Lipase]]~~ with sequence from [http://en.wikipedia.org/wiki/Candida_antarctica Candida antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA].	+	[[1tca]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_antarctica Candida antarctica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TCA OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:008087556~~</ref><ref group="xtra">PMID:011170211~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008087556</ref><references group="xtra"/>
	[[Category: Candida antarctica]]		[[Category: Candida antarctica]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Jones, T A.]]		[[Category: Jones, T A.]]
	[[Category: Uppenberg, J.]]		[[Category: Uppenberg, J.]]

OCA at 07:44, 27 July 2012

OCA — Fri, 27 Jul 2012 07:44:13 GMT

←Older revision		Revision as of 07:44, 27 July 2012
Line 1:		Line 1:
	[[Image:1tca.png\|left\|200px]]		[[Image:1tca.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1tca", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1tca\| PDB=1tca \| SCENE= }}		{{STRUCTURE_1tca\| PDB=1tca \| SCENE= }}

	===THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA===		===THE SEQUENCE, CRYSTAL STRUCTURE DETERMINATION AND REFINEMENT OF TWO CRYSTAL FORMS OF LIPASE B FROM CANDIDA ANTARCTICA===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_8087556}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 8087556 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_8087556}}		{{ABSTRACT_PUBMED_8087556}}

Line 22:		Line 11:

	==See Also==		==See Also==
-	*[[Lipase]]	+	*[[Lipase\|Lipase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~8087556~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008087556</ref><ref group="xtra">PMID:011170211</ref><references group="xtra"/>
	[[Category: Candida antarctica]]		[[Category: Candida antarctica]]
	[[Category: Triacylglycerol lipase]]		[[Category: Triacylglycerol lipase]]
	[[Category: Jones, T A.]]		[[Category: Jones, T A.]]
	[[Category: Uppenberg, J.]]		[[Category: Uppenberg, J.]]

OCA: Protected "1tca" [edit=sysop:move=sysop]

OCA — Mon, 27 Dec 2010 01:06:58 GMT

Protected "1tca" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:06, 27 December 2010