1tju - Revision history

OCA at 06:31, 23 August 2023

OCA — Wed, 23 Aug 2023 06:31:10 GMT

←Older revision		Revision as of 06:31, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1tju' size='340' side='right'caption='[[1tju]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1tju' size='340' side='right'caption='[[1tju]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tju]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Anapl Anapl~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TJU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tju]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TJU FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1hy1\|1hy1]]~~, [[~~1k7w~~\|~~1k7w~~]]~~, [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 ~~4.3.2.1] </span>~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tju OCA], [https://pdbe.org/1tju PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tju RCSB], [https://www.ebi.ac.uk/pdbsum/1tju PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tju ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tju OCA], [https://pdbe.org/1tju PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tju RCSB], [https://www.ebi.ac.uk/pdbsum/1tju PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tju ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>	+	[https://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Anapl]]~~	+	[[Category: Anas platyrhynchos]]
-	~~[[Category: Argininosuccinate lyase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Codding~~, P W~~]]	+	[[Category: Codding PW]]
-	[[Category: Horvatin, C]]	+	[[Category: Horvatin C]]
-	[[Category: Howell~~, P L~~]]	+	[[Category: Howell PL]]
-	[[Category: Lobsanov~~, Y D~~]]	+	[[Category: Lobsanov YD]]
-	[[Category: Sampaleanu~~, L M~~]]	+	[[Category: Sampaleanu LM]]
-	[[Category: Smith~~, G D~~]]	+	[[Category: Smith GD]]
-	[[Category: Tsai, M]]	+	[[Category: Tsai M]]
-	~~[[Category: Delta 2 crystallin]]~~	+
-	~~[[Category: Enzyme mechanism]]~~	+
-	~~[[Category: Eye lens protein]]~~	+
-	~~[[Category: Lyase~~]]	+

OCA at 09:28, 29 September 2021

OCA — Wed, 29 Sep 2021 09:28:43 GMT

←Older revision		Revision as of 09:28, 29 September 2021
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	<StructureSection load='1tju' size='340' side='right'caption='[[1tju]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1tju' size='340' side='right'caption='[[1tju]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tju]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1TJU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tju]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TJU FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1tju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tju OCA], [~~http~~://pdbe.org/1tju PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1tju RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1tju PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1tju ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tju OCA], [https://pdbe.org/1tju PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tju RCSB], [https://www.ebi.ac.uk/pdbsum/1tju PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tju ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>	+	[[https://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 13:32, 25 December 2019

OCA — Wed, 25 Dec 2019 13:32:07 GMT

←Older revision		Revision as of 13:32, 25 December 2019
Line 1:		Line 1:

	==Crystal Structure of T161S Duck Delta 2 Crystallin Mutant==		==Crystal Structure of T161S Duck Delta 2 Crystallin Mutant==
-	<StructureSection load='1tju' size='340' side='right' caption='[[1tju]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1tju' size='340' side='right'caption='[[1tju]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tju]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tju]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJU FirstGlance]. <br>
Line 29:		Line 29:
	</div>		</div>
	<div class="pdbe-citations 1tju" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1tju" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Crystallin 3D structures\|Crystallin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	[[Category: Anapl]]		[[Category: Anapl]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
		+	[[Category: Large Structures]]
	[[Category: Codding, P W]]		[[Category: Codding, P W]]
	[[Category: Horvatin, C]]		[[Category: Horvatin, C]]

OCA at 08:24, 21 March 2018

OCA — Wed, 21 Mar 2018 08:24:18 GMT

←Older revision		Revision as of 08:24, 21 March 2018
Line 1:		Line 1:
		+
	==Crystal Structure of T161S Duck Delta 2 Crystallin Mutant==		==Crystal Structure of T161S Duck Delta 2 Crystallin Mutant==
	<StructureSection load='1tju' size='340' side='right' caption='[[1tju]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1tju' size='340' side='right' caption='[[1tju]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 5:		Line 6:
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tju OCA], [http://pdbe.org/1tju PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tju RCSB], [http://www.ebi.ac.uk/pdbsum/1tju PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tju OCA], [http://pdbe.org/1tju PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tju RCSB], [http://www.ebi.ac.uk/pdbsum/1tju PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tju ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tj/1tju_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tj/1tju_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 23:13, 9 February 2016

OCA — Tue, 09 Feb 2016 23:13:14 GMT

←Older revision		Revision as of 23:13, 9 February 2016
Line 17:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tju ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:59, 11 September 2015

OCA — Fri, 11 Sep 2015 02:59:38 GMT

←Older revision		Revision as of 02:59, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1tju' size='340' side='right' caption='[[1tju]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1tju' size='340' side='right' caption='[[1tju]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tju]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Anas_platyrhynchos Anas platyrhynchos~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tju]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anapl Anapl]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJU FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tju OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tju RCSB], [http://www.ebi.ac.uk/pdbsum/1tju PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tju OCA], [http://pdbe.org/1tju PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tju RCSB], [http://www.ebi.ac.uk/pdbsum/1tju PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ARLY2_ANAPL ARLY2_ANAPL]] Delta crystallin, the principal crystallin in embryonic lens, is found only in birds and reptiles. This protein also functions as an enzymatically active argininosuccinate lyase.<ref>PMID:10029536</ref> <ref>PMID:11698398</ref> <ref>PMID:15320872</ref> <ref>PMID:9369472</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1tju" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Anas platyrhynchos~~]]	+	[[Category: Anapl]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
	[[Category: Codding, P W]]		[[Category: Codding, P W]]

OCA at 12:16, 6 January 2015

OCA — Tue, 06 Jan 2015 12:16:59 GMT

←Older revision		Revision as of 12:16, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tju]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tju]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tju OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tju RCSB], [http://www.ebi.ac.uk/pdbsum/1tju PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tju OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tju RCSB], [http://www.ebi.ac.uk/pdbsum/1tju PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 31:
	[[Category: Anas platyrhynchos]]		[[Category: Anas platyrhynchos]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
-	[[Category: Codding, P W.]]	+	[[Category: Codding, P W]]
-	[[Category: Horvatin, C.]]	+	[[Category: Horvatin, C]]
-	[[Category: Howell, P L.]]	+	[[Category: Howell, P L]]
-	[[Category: Lobsanov, Y D.]]	+	[[Category: Lobsanov, Y D]]
-	[[Category: Sampaleanu, L M.]]	+	[[Category: Sampaleanu, L M]]
-	[[Category: Smith, G D.]]	+	[[Category: Smith, G D]]
-	[[Category: Tsai, M~~.]]~~	+	[[Category: Tsai, M]]
-	~~[[Category: Argininosuccinate lyase~~]]	+
	[[Category: Delta 2 crystallin]]		[[Category: Delta 2 crystallin]]
	[[Category: Enzyme mechanism]]		[[Category: Enzyme mechanism]]
	[[Category: Eye lens protein]]		[[Category: Eye lens protein]]
	[[Category: Lyase]]		[[Category: Lyase]]

OCA at 21:15, 29 September 2014

OCA — Mon, 29 Sep 2014 21:15:50 GMT

←Older revision		Revision as of 21:15, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1tju.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of T161S Duck Delta 2 Crystallin Mutant==
		+	<StructureSection load='1tju' size='340' side='right' caption='[[1tju]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1tju]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TJU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1hy1\|1hy1]], [[1k7w\|1k7w]], [[1dcn\|1dcn]], [[1auw\|1auw]], [[1hy0\|1hy0]], [[1k62\|1k62]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Argininosuccinate_lyase Argininosuccinate lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.3.2.1 4.3.2.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tju FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tju OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tju RCSB], [http://www.ebi.ac.uk/pdbsum/1tju PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tj/1tju_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Delta crystallin, a taxon-specific crystallin present in avian eye lenses, is homologous to the urea cycle enzyme ASL (argininosuccinate lyase). Although there are two delta crystallin isoforms in duck lenses, ddeltac1 (duck delta1 crystallin) and ddeltac2 (duck delta2 crystallin), only ddeltac2 is catalytically active. Previous structural studies have suggested that residues Ser283 and His162 in the multi-subunit active site of ddeltac2/ASL are the putative catalytic acid/base, while the highly conserved, positively charged Lys289 is thought to help stabilize the carbanion intermediate. The strict conservation of a small hydroxy-containing residue (Thr or Ser) at position 161 adjacent to the putative catalytic base, as well as its proximity to the substrate in the S283A ddeltac2 enzyme-substrate complex, prompted us to investigate further the role this residue. Structures of the active T161S and inactive T161D ddeltac2 mutants, as well as T161D complexed with argininosuccinate, have been determined to 2.0 A resolution. The structures suggest that a hydroxy group is required at position 161 to help correctly position the side chain of Lys289 and the fumarate moiety of the substrate. Threonine is probably favoured over serine, because the interaction of its methyl group with Leu206 would restrict its conformational flexibility. Residues larger than Thr or Ser interfere with substrate binding, supporting previous suggestions that correct positioning of the substrate's fumarate moiety is essential for catalysis to occur. The presence of the 280s loop (i.e. a loop formed by residues 270-290) in the 'open' conformation suggests that loop closure, thought to be essential for sequestration of the substrate, may be triggered by the formation of the carbanion or aci-carboxylate intermediates, whose charge distribution more closely mimics that of the sulphate ion found in the active-site region of the inactive ddeltac1. The 280s loop in ddeltac1 is in the closed conformation.

-	~~{{STRUCTURE_1tju\| PDB=1tju \| SCENE= }}~~	+	Structural studies of duck delta2 crystallin mutants provide insight into the role of Thr161 and the 280s loop in catalysis.,Sampaleanu LM, Codding PW, Lobsanov YD, Tsai M, Smith GD, Horvatin C, Howell PL Biochem J. 2004 Dec 1;384(Pt 2):437-47. PMID:15320872<ref>PMID:15320872</ref>

-	~~===Crystal Structure~~ of ~~T161S Duck Delta 2 Crystallin Mutant===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15320872}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1tju]] is a 4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Anas_platyrhynchos Anas platyrhynchos]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015320872<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Anas platyrhynchos]]		[[Category: Anas platyrhynchos]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]

OCA: Protected "1tju" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 12:23:07 GMT

Protected "1tju" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:23, 5 January 2013

OCA at 12:23, 5 January 2013

OCA — Sat, 05 Jan 2013 12:23:03 GMT

←Older revision		Revision as of 12:23, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1tju.png\|left\|200px]]		[[Image:1tju.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1tju", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1tju\| PDB=1tju \| SCENE= }}		{{STRUCTURE_1tju\| PDB=1tju \| SCENE= }}

	===Crystal Structure of T161S Duck Delta 2 Crystallin Mutant===		===Crystal Structure of T161S Duck Delta 2 Crystallin Mutant===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15320872}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15320872 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15320872}}		{{ABSTRACT_PUBMED_15320872}}

	==About this Structure==		==About this Structure==
-	~~1TJU~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA].	+	[[1tju]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Anas_platyrhynchos Anas platyrhynchos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TJU OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15320872~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015320872</ref><references group="xtra"/>
	[[Category: Anas platyrhynchos]]		[[Category: Anas platyrhynchos]]
	[[Category: Argininosuccinate lyase]]		[[Category: Argininosuccinate lyase]]
Line 37:		Line 25:
	[[Category: Enzyme mechanism]]		[[Category: Enzyme mechanism]]
	[[Category: Eye lens protein]]		[[Category: Eye lens protein]]
-		+	[[Category: Lyase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 13:30:11 2009''~~	+