1tpz - Revision history

OCA at 13:30, 13 March 2024

OCA — Wed, 13 Mar 2024 13:30:19 GMT

←Older revision		Revision as of 13:30, 13 March 2024
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	<StructureSection load='1tpz' size='340' side='right'caption='[[1tpz]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1tpz' size='340' side='right'caption='[[1tpz]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tpz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1TPZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tpz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TPZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>~~</td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1f5n\|1f5n]], [[1dg3\|1dg3]], [[1tqd\|1tqd]], [[1tq2\|1tq2]], [[1tq4\|1tq4]], [[1tq6\|1tq6]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IIGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1tpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tpz OCA], [https://pdbe.org/1tpz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1tpz RCSB], [https://www.ebi.ac.uk/pdbsum/1tpz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1tpz ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1tpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tpz OCA], [~~http~~://pdbe.org/1tpz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1tpz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1tpz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1tpz ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/IIGP1_MOUSE IIGP1_MOUSE]] GTPase with low activity. Has higher affinity for GDP than for GTP. Plays a role in resistance to intracellular pathogens. Required for disruption of the parasitophorous vacuole formed following T.gondii infection and subsequent killing of the parasite. Mediates resistance to C.trachomatis infection by targeting bacterial inclusions to autophagosomes for subsequent lysosomal destruction.<ref>PMID:11907101</ref> <ref>PMID:16304607</ref> <ref>PMID:19242543</ref> <ref>PMID:12732635</ref>	+	[https://www.uniprot.org/uniprot/IIGP1_MOUSE IIGP1_MOUSE] GTPase with low activity. Has higher affinity for GDP than for GTP. Plays a role in resistance to intracellular pathogens. Required for disruption of the parasitophorous vacuole formed following T.gondii infection and subsequent killing of the parasite. Mediates resistance to C.trachomatis infection by targeting bacterial inclusions to autophagosomes for subsequent lysosomal destruction.<ref>PMID:11907101</ref> <ref>PMID:16304607</ref> <ref>PMID:19242543</ref> <ref>PMID:12732635</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tpz ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tpz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Interferon-inducible p47 GTPases are critical mediators of cell-autonomous resistance against several intracellular pathogens. Here we present the first crystal structure of a member of this novel GTPase family, IIGP1, in its nucleotide-free, GDP-, and GppNHp-bound form. The structure shows a Ras-like G domain between an N-terminal three-helix bundle and a complex system of C-terminal helices and loops. Sequence comparison and secondary structure prediction suggest the IIGP1 structure to be a valid model for the p47 GTPase family. The IIGP1 crystals contain a noncrystallographic dimer. We show that the dimer is required for cooperative GTP hydrolysis and GTP-dependent oligomerization of IIGP1. We also present the GDP- and GppNHp-bound monomeric structures of two dimer interface mutants. Our structures direct approaches to the analysis of the catalytic mechanism of IIGP1 and provide a coherent basis for structure-function studies aimed at elucidating the mechanistic basis of pathogen resistance caused by these enigmatic GTPases.
-
-	Crystal structure of IIGP1: a paradigm for interferon-inducible p47 resistance GTPases.,Ghosh A, Uthaiah R, Howard J, Herrmann C, Wolf E Mol Cell. 2004 Sep 10;15(5):727-39. PMID:15350217<ref>PMID:15350217</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1tpz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Ghosh, A]]	+	[[Category: Ghosh A]]
-	[[Category: Herrmann, C]]	+	[[Category: Herrmann C]]
-	[[Category: Howard, J]]	+	[[Category: Howard J]]
-	[[Category: Uthaiah, R]]	+	[[Category: Uthaiah R]]
-	[[Category: Wolf, E]]	+	[[Category: Wolf E]]
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Gtpase]]~~	+
-	~~[[Category: Immunology]]~~	+
-	~~[[Category: Interferon gamma]]~~	+
-	~~[[Category: Signaling protein~~]]	+

OCA at 11:57, 24 December 2020

OCA — Thu, 24 Dec 2020 11:57:38 GMT

←Older revision		Revision as of 11:57, 24 December 2020
Line 1:		Line 1:

	==Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases==		==Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases==
-	<StructureSection load='1tpz' size='340' side='right' caption='[[1tpz]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1tpz' size='340' side='right'caption='[[1tpz]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tpz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1TPZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tpz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1TPZ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f5n\|1f5n]], [[1dg3\|1dg3]], [[1tqd\|1tqd]], [[1tq2\|1tq2]], [[1tq4\|1tq4]], [[1tq6\|1tq6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1f5n\|1f5n]], [[1dg3\|1dg3]], [[1tqd\|1tqd]], [[1tq2\|1tq2]], [[1tq4\|1tq4]], [[1tq6\|1tq6]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IIGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IIGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1tpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tpz OCA], [http://pdbe.org/1tpz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tpz RCSB], [http://www.ebi.ac.uk/pdbsum/1tpz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tpz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1tpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tpz OCA], [http://pdbe.org/1tpz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tpz RCSB], [http://www.ebi.ac.uk/pdbsum/1tpz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tpz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 34:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Ghosh, A]]		[[Category: Ghosh, A]]

OCA at 08:44, 21 March 2018

OCA — Wed, 21 Mar 2018 08:44:05 GMT

←Older revision		Revision as of 08:44, 21 March 2018
Line 1:		Line 1:
		+
	==Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases==		==Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases==
	<StructureSection load='1tpz' size='340' side='right' caption='[[1tpz]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1tpz' size='340' side='right' caption='[[1tpz]], [[Resolution\|resolution]] 2.00Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f5n\|1f5n]], [[1dg3\|1dg3]], [[1tqd\|1tqd]], [[1tq2\|1tq2]], [[1tq4\|1tq4]], [[1tq6\|1tq6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f5n\|1f5n]], [[1dg3\|1dg3]], [[1tqd\|1tqd]], [[1tq2\|1tq2]], [[1tq4\|1tq4]], [[1tq6\|1tq6]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IIGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IIGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tpz OCA], [http://pdbe.org/1tpz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tpz RCSB], [http://www.ebi.ac.uk/pdbsum/1tpz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tpz OCA], [http://pdbe.org/1tpz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tpz RCSB], [http://www.ebi.ac.uk/pdbsum/1tpz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1tpz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tp/1tpz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tp/1tpz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 11:07, 7 February 2016

OCA — Sun, 07 Feb 2016 11:07:14 GMT

←Older revision		Revision as of 11:07, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1tpz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:54, 10 September 2015

OCA — Thu, 10 Sep 2015 00:54:39 GMT

←Older revision		Revision as of 00:54, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1tpz' size='340' side='right' caption='[[1tpz]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1tpz' size='340' side='right' caption='[[1tpz]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1tpz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TPZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1tpz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TPZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f5n\|1f5n]], [[1dg3\|1dg3]], [[1tqd\|1tqd]], [[1tq2\|1tq2]], [[1tq4\|1tq4]], [[1tq6\|1tq6]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f5n\|1f5n]], [[1dg3\|1dg3]], [[1tqd\|1tqd]], [[1tq2\|1tq2]], [[1tq4\|1tq4]], [[1tq6\|1tq6]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IIGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IIGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tpz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tpz RCSB], [http://www.ebi.ac.uk/pdbsum/1tpz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tpz OCA], [http://pdbe.org/1tpz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1tpz RCSB], [http://www.ebi.ac.uk/pdbsum/1tpz PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1tpz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Ghosh, A]]		[[Category: Ghosh, A]]
	[[Category: Herrmann, C]]		[[Category: Herrmann, C]]

OCA at 14:37, 24 December 2014

OCA — Wed, 24 Dec 2014 14:37:39 GMT

←Older revision		Revision as of 14:37, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1tpz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TPZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1tpz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TPZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f5n\|1f5n]], [[1dg3\|1dg3]], [[1tqd\|1tqd]], [[1tq2\|1tq2]], [[1tq4\|1tq4]], [[1tq6\|1tq6]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f5n\|1f5n]], [[1dg3\|1dg3]], [[1tqd\|1tqd]], [[1tq2\|1tq2]], [[1tq4\|1tq4]], [[1tq6\|1tq6]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IIGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IIGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tpz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tpz RCSB], [http://www.ebi.ac.uk/pdbsum/1tpz PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tpz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tpz RCSB], [http://www.ebi.ac.uk/pdbsum/1tpz PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/IIGP1_MOUSE IIGP1_MOUSE]] GTPase with low activity. Has higher affinity for GDP than for GTP. Plays a role in resistance to intracellular pathogens. Required for disruption of the parasitophorous vacuole formed following T.gondii infection and subsequent killing of the parasite. Mediates resistance to C.trachomatis infection by targeting bacterial inclusions to autophagosomes for subsequent lysosomal destruction.<ref>PMID:11907101</ref> <ref>PMID:16304607</ref> <ref>PMID:19242543</ref> <ref>PMID:12732635</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 31:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Ghosh, A.]]	+	[[Category: Ghosh, A]]
-	[[Category: Herrmann, C.]]	+	[[Category: Herrmann, C]]
-	[[Category: Howard, J.]]	+	[[Category: Howard, J]]
-	[[Category: Uthaiah, R.]]	+	[[Category: Uthaiah, R]]
-	[[Category: Wolf, E.]]	+	[[Category: Wolf, E]]
	[[Category: Dimer]]		[[Category: Dimer]]
	[[Category: Gtpase]]		[[Category: Gtpase]]

OCA at 20:32, 29 September 2014

OCA — Mon, 29 Sep 2014 20:32:04 GMT

←Older revision		Revision as of 20:32, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1tpz.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases==
		+	<StructureSection load='1tpz' size='340' side='right' caption='[[1tpz]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1tpz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TPZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1f5n\|1f5n]], [[1dg3\|1dg3]], [[1tqd\|1tqd]], [[1tq2\|1tq2]], [[1tq4\|1tq4]], [[1tq6\|1tq6]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">IIGP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1tpz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1tpz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1tpz RCSB], [http://www.ebi.ac.uk/pdbsum/1tpz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tp/1tpz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Interferon-inducible p47 GTPases are critical mediators of cell-autonomous resistance against several intracellular pathogens. Here we present the first crystal structure of a member of this novel GTPase family, IIGP1, in its nucleotide-free, GDP-, and GppNHp-bound form. The structure shows a Ras-like G domain between an N-terminal three-helix bundle and a complex system of C-terminal helices and loops. Sequence comparison and secondary structure prediction suggest the IIGP1 structure to be a valid model for the p47 GTPase family. The IIGP1 crystals contain a noncrystallographic dimer. We show that the dimer is required for cooperative GTP hydrolysis and GTP-dependent oligomerization of IIGP1. We also present the GDP- and GppNHp-bound monomeric structures of two dimer interface mutants. Our structures direct approaches to the analysis of the catalytic mechanism of IIGP1 and provide a coherent basis for structure-function studies aimed at elucidating the mechanistic basis of pathogen resistance caused by these enigmatic GTPases.

-	~~{{STRUCTURE_1tpz\| PDB=1tpz \| SCENE= }}~~	+	Crystal structure of IIGP1: a paradigm for interferon-inducible p47 resistance GTPases.,Ghosh A, Uthaiah R, Howard J, Herrmann C, Wolf E Mol Cell. 2004 Sep 10;15(5):727-39. PMID:15350217<ref>PMID:15350217</ref>

-	~~===Crystal Structure~~ of ~~IIGP1: a paradigm for interferon inducible p47 resistance GTPases===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15350217}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1tpz]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015350217<~~/~~ref~~><~~ref group="xtra">PMID:012732635</ref><references group="xtra"~~/>	+
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Ghosh, A.]]		[[Category: Ghosh, A.]]

OCA at 09:24, 16 January 2013

OCA — Wed, 16 Jan 2013 09:24:26 GMT

←Older revision		Revision as of 09:24, 16 January 2013
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	[[Image:1tpz.png\|left\|200px]]		[[Image:1tpz.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1tpz", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1tpz\| PDB=1tpz \| SCENE= }}		{{STRUCTURE_1tpz\| PDB=1tpz \| SCENE= }}

	===Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases===		===Crystal Structure of IIGP1: a paradigm for interferon inducible p47 resistance GTPases===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15350217}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15350217 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15350217}}		{{ABSTRACT_PUBMED_15350217}}

Line 22:		Line 11:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15350217~~</ref><ref group="xtra">PMID:~~12732635~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015350217</ref><ref group="xtra">PMID:012732635</ref><references group="xtra"/>
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
	[[Category: Ghosh, A.]]		[[Category: Ghosh, A.]]
Line 29:		Line 18:
	[[Category: Uthaiah, R.]]		[[Category: Uthaiah, R.]]
	[[Category: Wolf, E.]]		[[Category: Wolf, E.]]
-	[[Category: Crystal structure]]
	[[Category: Dimer]]		[[Category: Dimer]]
	[[Category: Gtpase]]		[[Category: Gtpase]]

OCA: Protected "1tpz" [edit=sysop:move=sysop]

OCA — Mon, 14 Mar 2011 18:41:10 GMT

Protected "1tpz" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:41, 14 March 2011

OCA at 18:41, 14 March 2011

OCA — Mon, 14 Mar 2011 18:41:08 GMT

←Older revision		Revision as of 18:41, 14 March 2011
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1tpz.png\|left\|200px]]		[[Image:1tpz.png\|left\|200px]]

Line 20:		Line 19:

	==About this Structure==		==About this Structure==
-	~~1TPZ~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA].	+	[[1tpz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TPZ OCA].

	==Reference==		==Reference==
Line 35:		Line 34:
	[[Category: Immunology]]		[[Category: Immunology]]
	[[Category: Interferon gamma]]		[[Category: Interferon gamma]]
-		+	[[Category: Signaling protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Mon Feb 16 13:23:26 2009''~~	+