1twb - Revision history

OCA at 06:34, 23 August 2023

OCA — Wed, 23 Aug 2023 06:34:30 GMT

←Older revision		Revision as of 06:34, 23 August 2023
Line 3:		Line 3:
	<StructureSection load='1twb' size='340' side='right'caption='[[1twb]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1twb' size='340' side='right'caption='[[1twb]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium~~ influenzae~~" lehmann and neumann 1896~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TWB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TWB FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~SSPB~~, ~~HI1440 (~~[~~https://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [https://pdbe.org/1twb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [https://www.ebi.ac.uk/pdbsum/1twb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1twb ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [https://pdbe.org/1twb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [https://www.ebi.ac.uk/pdbsum/1twb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1twb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SSPB_HAEIN SSPB_HAEIN]] Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth (By similarity). Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion (By similarity).	+	[https://www.uniprot.org/uniprot/SSPB_HAEIN SSPB_HAEIN] Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth (By similarity). Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1twb" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1twb" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Stringent starvation protein 3D structures\|Stringent starvation protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacterium~~ influenzae ~~lehmann and neumann 1896~~]]	+	[[Category: Haemophilus influenzae]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baker~~, T A~~]]	+	[[Category: Baker TA]]
-	[[Category: Bolon~~, D N~~]]	+	[[Category: Bolon DN]]
-	[[Category: Grant~~, R A~~]]	+	[[Category: Grant RA]]
-	[[Category: Sauer~~, R T]]~~	+	[[Category: Sauer RT]]
-	~~[[Category: Aaa+]]~~	+
-	~~[[Category: Adaptor]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Protease]]~~	+
-	~~[[Category: Specificity factor~~]]	+

OCA at 15:44, 27 October 2021

OCA — Wed, 27 Oct 2021 15:44:02 GMT

←Older revision		Revision as of 15:44, 27 October 2021
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	<StructureSection load='1twb' size='340' side='right'caption='[[1twb]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1twb' size='340' side='right'caption='[[1twb]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1TWB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TWB FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSPB, HI1440 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSPB, HI1440 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [~~http~~://pdbe.org/1twb PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1twb PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1twb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [https://pdbe.org/1twb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [https://www.ebi.ac.uk/pdbsum/1twb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1twb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SSPB_HAEIN SSPB_HAEIN]] Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth (By similarity). Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion (By similarity).	+	[[https://www.uniprot.org/uniprot/SSPB_HAEIN SSPB_HAEIN]] Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth (By similarity). Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 12:01, 24 December 2020

OCA — Thu, 24 Dec 2020 12:01:59 GMT

←Older revision		Revision as of 12:01, 24 December 2020
Line 1:		Line 1:

	==SspB disulfide crosslinked to an ssrA degradation tag==		==SspB disulfide crosslinked to an ssrA degradation tag==
-	<StructureSection load='1twb' size='340' side='right' caption='[[1twb]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1twb' size='340' side='right'caption='[[1twb]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1TWB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1TWB FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSPB, HI1440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSPB, HI1440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [http://pdbe.org/1twb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [http://www.ebi.ac.uk/pdbsum/1twb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1twb ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [http://pdbe.org/1twb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [http://www.ebi.ac.uk/pdbsum/1twb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1twb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Bacterium influenzae lehmann and neumann 1896]]		[[Category: Bacterium influenzae lehmann and neumann 1896]]
		+	[[Category: Large Structures]]
	[[Category: Baker, T A]]		[[Category: Baker, T A]]
	[[Category: Bolon, D N]]		[[Category: Bolon, D N]]

OCA at 08:45, 21 March 2018

OCA — Wed, 21 Mar 2018 08:45:26 GMT

←Older revision		Revision as of 08:45, 21 March 2018
Line 1:		Line 1:
		+
	==SspB disulfide crosslinked to an ssrA degradation tag==		==SspB disulfide crosslinked to an ssrA degradation tag==
	<StructureSection load='1twb' size='340' side='right' caption='[[1twb]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1twb' size='340' side='right' caption='[[1twb]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWB FirstGlance]. <br>
	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSPB, HI1440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>		</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSPB, HI1440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [http://pdbe.org/1twb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [http://www.ebi.ac.uk/pdbsum/1twb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [http://pdbe.org/1twb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [http://www.ebi.ac.uk/pdbsum/1twb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1twb ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tw/1twb_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tw/1twb_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 23:09, 7 February 2016

OCA — Sun, 07 Feb 2016 23:09:23 GMT

←Older revision		Revision as of 23:09, 7 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1twb ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:43, 10 September 2015

OCA — Thu, 10 Sep 2015 05:43:01 GMT

←Older revision		Revision as of 05:43, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1twb' size='340' side='right' caption='[[1twb]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1twb' size='340' side='right' caption='[[1twb]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Haemophilus_influenzae Haemophilus~~ influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWB FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacterium_influenzae"_lehmann_and_neumann_1896 "bacterium influenzae" lehmann and neumann 1896]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWB FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSPB, HI1440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 ~~Haemophilus~~ influenzae])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSPB, HI1440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 "Bacterium influenzae" Lehmann and Neumann 1896])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [http://www.ebi.ac.uk/pdbsum/1twb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [http://pdbe.org/1twb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [http://www.ebi.ac.uk/pdbsum/1twb PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1twb" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Haemophilus~~ influenzae]]	+	[[Category: Bacterium influenzae lehmann and neumann 1896]]
	[[Category: Baker, T A]]		[[Category: Baker, T A]]
	[[Category: Bolon, D N]]		[[Category: Bolon, D N]]

OCA at 18:50, 24 December 2014

OCA — Wed, 24 Dec 2014 18:50:13 GMT

←Older revision		Revision as of 18:50, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWB FirstGlance]. <br>		<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWB FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSPB, HI1440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])</td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSPB, HI1440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [http://www.ebi.ac.uk/pdbsum/1twb PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [http://www.ebi.ac.uk/pdbsum/1twb PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SSPB_HAEIN SSPB_HAEIN]] Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth (By similarity). Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Haemophilus influenzae]]		[[Category: Haemophilus influenzae]]
-	[[Category: Baker, T A.]]	+	[[Category: Baker, T A]]
-	[[Category: Bolon, D N.]]	+	[[Category: Bolon, D N]]
-	[[Category: Grant, R A.]]	+	[[Category: Grant, R A]]
-	[[Category: Sauer, R T.]]	+	[[Category: Sauer, R T]]
	[[Category: Aaa+]]		[[Category: Aaa+]]
	[[Category: Adaptor]]		[[Category: Adaptor]]

OCA at 19:35, 29 September 2014

OCA — Mon, 29 Sep 2014 19:35:16 GMT

←Older revision		Revision as of 19:35, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1twb.~~png\|left~~\|200px]]	+	==SspB disulfide crosslinked to an ssrA degradation tag==
		+	<StructureSection load='1twb' size='340' side='right' caption='[[1twb]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1twb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TWB FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SSPB, HI1440 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1twb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1twb OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1twb RCSB], [http://www.ebi.ac.uk/pdbsum/1twb PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tw/1twb_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The SspB adaptor enhances ClpXP degradation by binding the ssrA degradation tag of substrates and the AAA+ ClpX unfoldase. To probe the mechanism of substrate delivery, we engineered a disulfide bond between the ssrA tag and SspB and demonstrated otherwise normal interactions by solving the crystal structure. Although the covalent link prevents adaptor.substrate dissociation, ClpXP degraded GFP-ssrA that was disulfide bonded to the adaptor. Thus, crosslinked substrate must be handed directly from SspB to ClpX. The ssrA tag in the covalent adaptor complex interacted with ClpX.ATPgammaS but not ClpX.ADP, suggesting that handoff occurs in the ATP bound enzyme. By contrast, SspB alone bound ClpX in both nucleotide states. Similar handoff mechanisms will undoubtedly be used by many AAA+ adaptors and enzymes, allowing assembly of delivery complexes in either nucleotide state, engagement of the recognition tag in the ATP state, and application of an unfolding force to the attached protein following hydrolysis.

-	~~{{STRUCTURE_1twb\| PDB=1twb \| SCENE= }}~~	+	Nucleotide-dependent substrate handoff from the SspB adaptor to the AAA+ ClpXP protease.,Bolon DN, Grant RA, Baker TA, Sauer RT Mol Cell. 2004 Nov 5;16(3):343-50. PMID:15525508<ref>PMID:15525508</ref>

-	~~===SspB disulfide crosslinked to an ssrA degradation tag===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15525508}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1twb]] is~~ a ~~4 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Haemophilus_influenzae Haemophilus influenzae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015525508<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Haemophilus influenzae]]		[[Category: Haemophilus influenzae]]
	[[Category: Baker, T A.]]		[[Category: Baker, T A.]]

OCA: Protected "1twb" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 12:46:24 GMT

Protected "1twb" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:46, 5 January 2013

OCA at 12:46, 5 January 2013

OCA — Sat, 05 Jan 2013 12:46:21 GMT

←Older revision		Revision as of 12:46, 5 January 2013
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-	{{Seed}}
	[[Image:1twb.png\|left\|200px]]		[[Image:1twb.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1twb", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1twb\| PDB=1twb \| SCENE= }}		{{STRUCTURE_1twb\| PDB=1twb \| SCENE= }}

	===SspB disulfide crosslinked to an ssrA degradation tag===		===SspB disulfide crosslinked to an ssrA degradation tag===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15525508}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15525508 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15525508}}		{{ABSTRACT_PUBMED_15525508}}

	==About this Structure==		==About this Structure==
-	~~1TWB~~ is a 4 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA].	+	[[1twb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Haemophilus_influenzae Haemophilus influenzae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TWB OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15525508~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015525508</ref><references group="xtra"/>
	[[Category: Haemophilus influenzae]]		[[Category: Haemophilus influenzae]]
	[[Category: Baker, T A.]]		[[Category: Baker, T A.]]
Line 31:		Line 19:
	[[Category: Aaa+]]		[[Category: Aaa+]]
	[[Category: Adaptor]]		[[Category: Adaptor]]
		+	[[Category: Hydrolase]]
	[[Category: Protease]]		[[Category: Protease]]
	[[Category: Specificity factor]]		[[Category: Specificity factor]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:06:12 2009''