1txj - Revision history

OCA at 08:42, 14 February 2024

OCA — Wed, 14 Feb 2024 08:42:39 GMT

←Older revision		Revision as of 08:42, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1txj' size='340' side='right'caption='[[1txj]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1txj' size='340' side='right'caption='[[1txj]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Plakn Plakn~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plasmodium_knowlesi Plasmodium knowlesi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1txj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txj OCA], [https://pdbe.org/1txj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1txj RCSB], [https://www.ebi.ac.uk/pdbsum/1txj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1txj ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1txj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txj OCA], [https://pdbe.org/1txj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1txj RCSB], [https://www.ebi.ac.uk/pdbsum/1txj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1txj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/TCTP_PLAKN TCTP_PLAKN]] Involved in calcium binding and microtubule stabilization (By similarity).	+	[https://www.uniprot.org/uniprot/TCTP_PLAKN TCTP_PLAKN] Involved in calcium binding and microtubule stabilization (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1txj ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1txj ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Parasites from the protozoan phylum Apicomplexa are responsible for diseases, such as malaria, toxoplasmosis and cryptosporidiosis, all of which have significantly higher rates of mortality and morbidity in economically underdeveloped regions of the world. Advances in vaccine development and drug discovery are urgently needed to control these diseases and can be facilitated by production of purified recombinant proteins from Apicomplexan genomes and determination of their 3D structures. To date, both heterologous expression and crystallization of Apicomplexan proteins have seen only limited success. In an effort to explore the effectiveness of producing and crystallizing proteins on a genome-scale using a standardized methodology, over 400 distinct Plasmodium falciparum target genes were chosen representing different cellular classes, along with select orthologues from four other Plasmodium species as well as Cryptosporidium parvum and Toxoplasma gondii. From a total of 1008 genes from the seven genomes, 304 (30.2%) produced purified soluble proteins and 97 (9.6%) crystallized, culminating in 36 crystal structures. These results demonstrate that, contrary to previous findings, a standardized platform using Escherichia coli can be effective for genome-scale production and crystallography of Apicomplexan proteins. Predictably, orthologous proteins from different Apicomplexan genomes behaved differently in expression, purification and crystallization, although the overall success rates of Plasmodium orthologues do not differ significantly. Their differences were effectively exploited to elevate the overall productivity to levels comparable to the most successful ongoing structural genomics projects: 229 of the 468 target genes produced purified soluble protein from one or more organisms, with 80 and 32 of the purified targets, respectively, leading to crystals and ultimately structures from one or more orthologues.
-
-	Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms.,Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, Qiu W, Sharma S, Diassiti A, Alam Z, Melone M, Mulichak A, Wernimont A, Bray J, Loppnau P, Plotnikova O, Newberry K, Sundararajan E, Houston S, Walker J, Tempel W, Bochkarev A, Kozieradzki I, Edwards A, Arrowsmith C, Roos D, Kain K, Hui R Mol Biochem Parasitol. 2007 Jan;151(1):100-10. Epub 2006 Nov 13. PMID:17125854<ref>PMID:17125854</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1txj" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Plakn~~]]	+	[[Category: Plasmodium knowlesi]]
-	[[Category: Amani, M]]	+	[[Category: Amani M]]
-	[[Category: Arrowsmith, C]]	+	[[Category: Arrowsmith C]]
-	[[Category: Bray, J]]	+	[[Category: Bray J]]
-	[[Category: Edwards, A]]	+	[[Category: Edwards A]]
-	[[Category: Houston, S]]	+	[[Category: Houston S]]
-	[[Category: Hui, R]]	+	[[Category: Hui R]]
-	[[Category: Lew, J]]	+	[[Category: Lew J]]
-	~~[[Category: Structural genomic~~]]	+	[[Category: Sharma S]]
-	[[Category: Sharma, S]]	+	[[Category: Skarina T]]
-	[[Category: Skarina, T]]	+	[[Category: Sundstrom M]]
-	[[Category: Sundstrom, M]]	+	[[Category: Vedadi M]]
-	[[Category: Vedadi, M]]	+	[[Category: Walker JR]]
-	[[Category: Walker~~, J R~~]]	+	[[Category: Wasney G]]
-	[[Category: Wasney, G]]	+
-	~~[[Category: Beta-sandwich]]~~	+
-	~~[[Category: Guanine nucleotide exchange factor]]~~	+
-	~~[[Category: Guanine nucleotide-free chaperone]]~~	+
-	~~[[Category: Helix-turn-helix]]~~	+
-	~~[[Category: Sgc]]~~	+
-	~~[[Category: Unknown function~~]]	+

OCA at 09:33, 29 September 2021

OCA — Wed, 29 Sep 2021 09:33:55 GMT

←Older revision		Revision as of 09:33, 29 September 2021
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	<StructureSection load='1txj' size='340' side='right'caption='[[1txj]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1txj' size='340' side='right'caption='[[1txj]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Plakn Plakn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Plakn Plakn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1txj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txj OCA], [~~http~~://pdbe.org/1txj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1txj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1txj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1txj ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1txj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txj OCA], [https://pdbe.org/1txj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1txj RCSB], [https://www.ebi.ac.uk/pdbsum/1txj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1txj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/TCTP_PLAKN TCTP_PLAKN]] Involved in calcium binding and microtubule stabilization (By similarity).	+	[[https://www.uniprot.org/uniprot/TCTP_PLAKN TCTP_PLAKN]] Involved in calcium binding and microtubule stabilization (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 14:00, 25 December 2019

OCA — Wed, 25 Dec 2019 14:00:27 GMT

←Older revision		Revision as of 14:00, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of translationally controlled tumour-associated protein (TCTP) from Plasmodium knowlesi==		==Crystal structure of translationally controlled tumour-associated protein (TCTP) from Plasmodium knowlesi==
-	<StructureSection load='1txj' size='340' side='right' caption='[[1txj]], [[Resolution\|resolution]] 2.00Å' scene=''>	+	<StructureSection load='1txj' size='340' side='right'caption='[[1txj]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plakn Plakn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plakn Plakn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>
Line 31:		Line 31:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Plakn]]		[[Category: Plakn]]
	[[Category: Amani, M]]		[[Category: Amani, M]]

OCA at 08:50, 21 March 2018

OCA — Wed, 21 Mar 2018 08:50:13 GMT

←Older revision		Revision as of 08:50, 21 March 2018
Line 1:		Line 1:
		+
	==Crystal structure of translationally controlled tumour-associated protein (TCTP) from Plasmodium knowlesi==		==Crystal structure of translationally controlled tumour-associated protein (TCTP) from Plasmodium knowlesi==
	<StructureSection load='1txj' size='340' side='right' caption='[[1txj]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1txj' size='340' side='right' caption='[[1txj]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plakn Plakn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plakn Plakn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1txj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txj OCA], [http://pdbe.org/1txj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1txj RCSB], [http://www.ebi.ac.uk/pdbsum/1txj PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1txj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txj OCA], [http://pdbe.org/1txj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1txj RCSB], [http://www.ebi.ac.uk/pdbsum/1txj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1txj ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tx/1txj_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tx/1txj_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 00:10, 10 February 2016

OCA — Wed, 10 Feb 2016 00:10:02 GMT

←Older revision		Revision as of 00:10, 10 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1txj ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 03:25, 11 September 2015

OCA — Fri, 11 Sep 2015 03:25:22 GMT

←Older revision		Revision as of 03:25, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1txj' size='340' side='right' caption='[[1txj]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='1txj' size='340' side='right' caption='[[1txj]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Plasmodium_knowlesi Plasmodium knowlesi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plakn Plakn]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1txj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1txj RCSB], [http://www.ebi.ac.uk/pdbsum/1txj PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1txj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txj OCA], [http://pdbe.org/1txj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1txj RCSB], [http://www.ebi.ac.uk/pdbsum/1txj PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1txj" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Plasmodium knowlesi~~]]	+	[[Category: Plakn]]
	[[Category: Amani, M]]		[[Category: Amani, M]]
	[[Category: Arrowsmith, C]]		[[Category: Arrowsmith, C]]

OCA at 10:27, 25 December 2014

OCA — Thu, 25 Dec 2014 10:27:31 GMT

←Older revision		Revision as of 10:27, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_knowlesi Plasmodium knowlesi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_knowlesi Plasmodium knowlesi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1txj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1txj RCSB], [http://www.ebi.ac.uk/pdbsum/1txj PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1txj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1txj RCSB], [http://www.ebi.ac.uk/pdbsum/1txj PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TCTP_PLAKN TCTP_PLAKN]] Involved in calcium binding and microtubule stabilization (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Plasmodium knowlesi]]		[[Category: Plasmodium knowlesi]]
-	[[Category: Amani, M.]]	+	[[Category: Amani, M]]
-	[[Category: Arrowsmith, C.]]	+	[[Category: Arrowsmith, C]]
-	[[Category: Bray, J.]]	+	[[Category: Bray, J]]
-	[[Category: Edwards, A.]]	+	[[Category: Edwards, A]]
-	[[Category: Houston, S.]]	+	[[Category: Houston, S]]
-	[[Category: Hui, R.]]	+	[[Category: Hui, R]]
-	[[Category: Lew, J.]]	+	[[Category: Lew, J]]
-	[[Category: ~~SGC,~~ Structural ~~Genomics Consortium.~~]]	+	[[Category: Structural genomic]]
-	[[Category: Sharma, S.]]	+	[[Category: Sharma, S]]
-	[[Category: Skarina, T.]]	+	[[Category: Skarina, T]]
-	[[Category: Sundstrom, M.]]	+	[[Category: Sundstrom, M]]
-	[[Category: Vedadi, M.]]	+	[[Category: Vedadi, M]]
-	[[Category: Walker, J R.]]	+	[[Category: Walker, J R]]
-	[[Category: Wasney, G.]]	+	[[Category: Wasney, G]]
	[[Category: Beta-sandwich]]		[[Category: Beta-sandwich]]
	[[Category: Guanine nucleotide exchange factor]]		[[Category: Guanine nucleotide exchange factor]]
Line 47:		Line 49:
	[[Category: Helix-turn-helix]]		[[Category: Helix-turn-helix]]
	[[Category: Sgc]]		[[Category: Sgc]]
-	[[Category: Structural genomic]]
-	[[Category: Structural genomics consortium]]
	[[Category: Unknown function]]		[[Category: Unknown function]]

OCA at 21:32, 29 September 2014

OCA — Mon, 29 Sep 2014 21:32:58 GMT

←Older revision		Revision as of 21:32, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1txj.~~png\|left~~\|200px]]	+	==Crystal structure of translationally controlled tumour-associated protein (TCTP) from Plasmodium knowlesi==
		+	<StructureSection load='1txj' size='340' side='right' caption='[[1txj]], [[Resolution\|resolution]] 2.00Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1txj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_knowlesi Plasmodium knowlesi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1TXJ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1txj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1txj OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1txj RCSB], [http://www.ebi.ac.uk/pdbsum/1txj PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/tx/1txj_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Parasites from the protozoan phylum Apicomplexa are responsible for diseases, such as malaria, toxoplasmosis and cryptosporidiosis, all of which have significantly higher rates of mortality and morbidity in economically underdeveloped regions of the world. Advances in vaccine development and drug discovery are urgently needed to control these diseases and can be facilitated by production of purified recombinant proteins from Apicomplexan genomes and determination of their 3D structures. To date, both heterologous expression and crystallization of Apicomplexan proteins have seen only limited success. In an effort to explore the effectiveness of producing and crystallizing proteins on a genome-scale using a standardized methodology, over 400 distinct Plasmodium falciparum target genes were chosen representing different cellular classes, along with select orthologues from four other Plasmodium species as well as Cryptosporidium parvum and Toxoplasma gondii. From a total of 1008 genes from the seven genomes, 304 (30.2%) produced purified soluble proteins and 97 (9.6%) crystallized, culminating in 36 crystal structures. These results demonstrate that, contrary to previous findings, a standardized platform using Escherichia coli can be effective for genome-scale production and crystallography of Apicomplexan proteins. Predictably, orthologous proteins from different Apicomplexan genomes behaved differently in expression, purification and crystallization, although the overall success rates of Plasmodium orthologues do not differ significantly. Their differences were effectively exploited to elevate the overall productivity to levels comparable to the most successful ongoing structural genomics projects: 229 of the 468 target genes produced purified soluble protein from one or more organisms, with 80 and 32 of the purified targets, respectively, leading to crystals and ultimately structures from one or more orthologues.

-	~~{{STRUCTURE_1txj\| PDB=1txj \| SCENE= }}~~	+	Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms.,Vedadi M, Lew J, Artz J, Amani M, Zhao Y, Dong A, Wasney GA, Gao M, Hills T, Brokx S, Qiu W, Sharma S, Diassiti A, Alam Z, Melone M, Mulichak A, Wernimont A, Bray J, Loppnau P, Plotnikova O, Newberry K, Sundararajan E, Houston S, Walker J, Tempel W, Bochkarev A, Kozieradzki I, Edwards A, Arrowsmith C, Roos D, Kain K, Hui R Mol Biochem Parasitol. 2007 Jan;151(1):100-10. Epub 2006 Nov 13. PMID:17125854<ref>PMID:17125854</ref>

-	~~===Crystal structure~~ of ~~translationally controlled tumour-associated protein (TCTP) from Plasmodium knowlesi===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_17125854}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1txj]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Plasmodium_knowlesi Plasmodium knowlesi]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:017125854<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Plasmodium knowlesi]]		[[Category: Plasmodium knowlesi]]
	[[Category: Amani, M.]]		[[Category: Amani, M.]]

OCA: Protected "1txj" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 12:51:14 GMT

Protected "1txj" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:51, 5 January 2013

OCA at 12:51, 5 January 2013

OCA — Sat, 05 Jan 2013 12:51:11 GMT

←Older revision		Revision as of 12:51, 5 January 2013
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-	{{Seed}}
	[[Image:1txj.png\|left\|200px]]		[[Image:1txj.png\|left\|200px]]

-	<!--
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-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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	{{STRUCTURE_1txj\| PDB=1txj \| SCENE= }}		{{STRUCTURE_1txj\| PDB=1txj \| SCENE= }}

	===Crystal structure of translationally controlled tumour-associated protein (TCTP) from Plasmodium knowlesi===		===Crystal structure of translationally controlled tumour-associated protein (TCTP) from Plasmodium knowlesi===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_17125854}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 17125854 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_17125854}}		{{ABSTRACT_PUBMED_17125854}}

	==About this Structure==		==About this Structure==
-	~~1TXJ~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Plasmodium_knowlesi Plasmodium knowlesi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA].	+	[[1txj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Plasmodium_knowlesi Plasmodium knowlesi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1TXJ OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~17125854~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:017125854</ref><references group="xtra"/>
	[[Category: Plasmodium knowlesi]]		[[Category: Plasmodium knowlesi]]
	[[Category: Amani, M.]]		[[Category: Amani, M.]]
Line 44:		Line 32:
	[[Category: Helix-turn-helix]]		[[Category: Helix-turn-helix]]
	[[Category: Sgc]]		[[Category: Sgc]]
		+	[[Category: Structural genomic]]
	[[Category: Structural genomics consortium]]		[[Category: Structural genomics consortium]]
-		+	[[Category: Unknown function]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 13:50:59 2009''~~	+