1u0g - Revision history

OCA at 08:43, 14 February 2024

OCA — Wed, 14 Feb 2024 08:43:12 GMT

←Older revision		Revision as of 08:43, 14 February 2024
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	<StructureSection load='1u0g' size='340' side='right'caption='[[1u0g]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1u0g' size='340' side='right'caption='[[1u0g]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1u0g]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Lk3_transgenic_mice Lk3 transgenic mice~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1U0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1u0g]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0G FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BME:BETA~~-~~MERCAPTOETHANOL'>BME</scene>~~, ~~<scene name='pdbligand=E4P:ERYTHOSE-4-PHOSPHATE'>E4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1u0e\|1u0e]], [[1u0f\|1u0f]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=E4P:ERYTHOSE-4-PHOSPHATE'>E4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td><~~td class~~=~~"sblockDat">Gpi ([http~~:~~//www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td~~></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0g OCA], [https://pdbe.org/1u0g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0g RCSB], [https://www.ebi.ac.uk/pdbsum/1u0g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0g ProSAT]</span></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1u0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0g OCA], [~~http~~://pdbe.org/1u0g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1u0g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1u0g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0g ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/G6PI_MOUSE G6PI_MOUSE]] Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.	+	[https://www.uniprot.org/uniprot/G6PI_MOUSE G6PI_MOUSE] Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u0g ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u0g ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Phosphoglucose isomerase (PGI) is an enzyme of glycolysis that interconverts glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P) but, outside the cell, is a multifunctional cytokine. High-resolution crystal structures of the enzyme from mouse have been determined in native form and in complex with the inhibitor erythrose 4-phosphate, and with the substrate glucose 6-phosphate. In the substrate-bound structure, the glucose sugar is observed in both straight-chain and ring forms. This structure supports a specific role for Lys518 in enzyme-catalyzed ring opening and we present a "push-pull" mechanism in which His388 breaks the O5-C1 bond by donating a proton to the ring oxygen atom and, simultaneously, Lys518 abstracts a proton from the C1 hydroxyl group. The reverse occurs in ring closure. The transition from ring form to straight-chain substrate is achieved through rotation of the C3-C4 bond, which brings the C1-C2 region into close proximity to Glu357, the base catalyst for the isomerization step. The structure with G6P also explains the specificity of PGI for glucose 6-phosphate over mannose 6-isomerase (M6P). To isomerize M6P to F6P requires a rotation of its C2-C3 bond but in PGI this is sterically blocked by Gln511.
-
-	The crystal structure of mouse phosphoglucose isomerase at 1.6A resolution and its complex with glucose 6-phosphate reveals the catalytic mechanism of sugar ring opening.,Graham Solomons JT, Zimmerly EM, Burns S, Krishnamurthy N, Swan MK, Krings S, Muirhead H, Chirgwin J, Davies C J Mol Biol. 2004 Sep 17;342(3):847-60. PMID:15342241<ref>PMID:15342241</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1u0g" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
-	*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]
	*[[Phosphoglucose isomerase 3D structures\|Phosphoglucose isomerase 3D structures]]		*[[Phosphoglucose isomerase 3D structures\|Phosphoglucose isomerase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Glucose-6-phosphate isomerase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Lk3 transgenic mice~~]]	+	[[Category: Mus musculus]]
-	[[Category: Burns, S]]	+	[[Category: Burns S]]
-	[[Category: Chirgwin, J]]	+	[[Category: Chirgwin J]]
-	[[Category: Davies, C]]	+	[[Category: Davies C]]
-	[[Category: Krings, S]]	+	[[Category: Krings S]]
-	[[Category: Krishnamurthy, N]]	+	[[Category: Krishnamurthy N]]
-	[[Category: Muirhead, H]]	+	[[Category: Muirhead H]]
-	[[Category: Solomons~~, J T.G~~]]	+	[[Category: Solomons JTG]]
-	[[Category: Swan~~, M K~~]]	+	[[Category: Swan MK]]
-	[[Category: Zimmerly~~, E M]]~~	+	[[Category: Zimmerly EM]]
-	~~[[Category: Aldose-ketose isomerase]]~~	+
-	~~[[Category: Dimer]]~~	+
-	~~[[Category: Isomerase~~]]	+

OCA at 07:17, 30 December 2020

OCA — Wed, 30 Dec 2020 07:17:46 GMT

←Older revision		Revision as of 07:17, 30 December 2020
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	==Crystal structure of mouse phosphoglucose isomerase in complex with erythrose 4-phosphate==		==Crystal structure of mouse phosphoglucose isomerase in complex with erythrose 4-phosphate==
-	<StructureSection load='1u0g' size='340' side='right' caption='[[1u0g]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='1u0g' size='340' side='right'caption='[[1u0g]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1u0g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1U0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1u0g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1U0G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=E4P:ERYTHOSE-4-PHOSPHATE'>E4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=E4P:ERYTHOSE-4-PHOSPHATE'>E4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1u0e\|1u0e]], [[1u0f\|1u0f]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1u0e\|1u0e]], [[1u0f\|1u0f]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gpi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gpi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1u0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0g OCA], [http://pdbe.org/1u0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u0g RCSB], [http://www.ebi.ac.uk/pdbsum/1u0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0g ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1u0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0g OCA], [http://pdbe.org/1u0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u0g RCSB], [http://www.ebi.ac.uk/pdbsum/1u0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u0g_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u0g_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	==See Also==		==See Also==
	*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]		*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]
		+	*[[Phosphoglucose isomerase 3D structures\|Phosphoglucose isomerase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Glucose-6-phosphate isomerase]]		[[Category: Glucose-6-phosphate isomerase]]
		+	[[Category: Large Structures]]
	[[Category: Lk3 transgenic mice]]		[[Category: Lk3 transgenic mice]]
	[[Category: Burns, S]]		[[Category: Burns, S]]

OCA at 13:59, 12 October 2017

OCA — Thu, 12 Oct 2017 13:59:13 GMT

←Older revision		Revision as of 13:59, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of mouse phosphoglucose isomerase in complex with erythrose 4-phosphate==		==Crystal structure of mouse phosphoglucose isomerase in complex with erythrose 4-phosphate==
	<StructureSection load='1u0g' size='340' side='right' caption='[[1u0g]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1u0g' size='340' side='right' caption='[[1u0g]], [[Resolution\|resolution]] 1.70Å' scene=''>
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	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gpi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gpi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0g OCA], [http://pdbe.org/1u0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u0g RCSB], [http://www.ebi.ac.uk/pdbsum/1u0g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0g OCA], [http://pdbe.org/1u0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u0g RCSB], [http://www.ebi.ac.uk/pdbsum/1u0g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 19:		Line 20:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u0g ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
Line 33:		Line 34:
	==See Also==		==See Also==
	*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]		*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]
-	*[[Phosphoglucose isomerase\|Phosphoglucose isomerase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 12:22, 11 September 2015

OCA — Fri, 11 Sep 2015 12:22:14 GMT

←Older revision		Revision as of 12:22, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1u0g' size='340' side='right' caption='[[1u0g]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1u0g' size='340' side='right' caption='[[1u0g]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1u0g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Mus_musculus Mus musculus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1u0g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=E4P:ERYTHOSE-4-PHOSPHATE'>E4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=E4P:ERYTHOSE-4-PHOSPHATE'>E4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1u0e\|1u0e]], [[1u0f\|1u0f]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1u0e\|1u0e]], [[1u0f\|1u0f]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gpi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 ~~Mus musculus~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gpi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u0g RCSB], [http://www.ebi.ac.uk/pdbsum/1u0g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0g OCA], [http://pdbe.org/1u0g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u0g RCSB], [http://www.ebi.ac.uk/pdbsum/1u0g PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1u0g" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 38:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Glucose-6-phosphate isomerase]]		[[Category: Glucose-6-phosphate isomerase]]
-	[[Category: ~~Mus musculus~~]]	+	[[Category: Lk3 transgenic mice]]
	[[Category: Burns, S]]		[[Category: Burns, S]]
	[[Category: Chirgwin, J]]		[[Category: Chirgwin, J]]

OCA at 16:23, 25 December 2014

OCA — Thu, 25 Dec 2014 16:23:03 GMT

←Older revision		Revision as of 16:23, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1u0g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0G FirstGlance]. <br>		<table><tr><td colspan='2'>[[1u0g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0G FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=E4P:ERYTHOSE-4-PHOSPHATE'>E4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=E4P:ERYTHOSE-4-PHOSPHATE'>E4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1u0e\|1u0e]], [[1u0f\|1u0f]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1u0e\|1u0e]], [[1u0f\|1u0f]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gpi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gpi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u0g RCSB], [http://www.ebi.ac.uk/pdbsum/1u0g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u0g RCSB], [http://www.ebi.ac.uk/pdbsum/1u0g PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/G6PI_MOUSE G6PI_MOUSE]] Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 37:		Line 39:
	[[Category: Glucose-6-phosphate isomerase]]		[[Category: Glucose-6-phosphate isomerase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
-	[[Category: Burns, S.]]	+	[[Category: Burns, S]]
-	[[Category: Chirgwin, J.]]	+	[[Category: Chirgwin, J]]
-	[[Category: Davies, C.]]	+	[[Category: Davies, C]]
-	[[Category: Krings, S.]]	+	[[Category: Krings, S]]
-	[[Category: Krishnamurthy, N.]]	+	[[Category: Krishnamurthy, N]]
-	[[Category: Muirhead, H.]]	+	[[Category: Muirhead, H]]
-	[[Category: Solomons, J T.G.]]	+	[[Category: Solomons, J T.G]]
-	[[Category: Swan, M K.]]	+	[[Category: Swan, M K]]
-	[[Category: Zimmerly, E M.]]	+	[[Category: Zimmerly, E M]]
	[[Category: Aldose-ketose isomerase]]		[[Category: Aldose-ketose isomerase]]
	[[Category: Dimer]]		[[Category: Dimer]]
	[[Category: Isomerase]]		[[Category: Isomerase]]

OCA at 19:38, 29 September 2014

OCA — Mon, 29 Sep 2014 19:38:28 GMT

←Older revision		Revision as of 19:38, 29 September 2014
Line 1:		Line 1:
-	~~{{STRUCTURE_1u0g\| PDB=1u0g \| SCENE= }}~~	+	==Crystal structure of mouse phosphoglucose isomerase in complex with erythrose 4-phosphate==
-	===Crystal structure of mouse phosphoglucose isomerase in complex with erythrose 4-phosphate===	+	<StructureSection load='1u0g' size='340' side='right' caption='[[1u0g]], [[Resolution\|resolution]] 1.70Å' scene=''>
-	~~{{ABSTRACT_PUBMED_15342241}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1u0g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U0G FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=E4P:ERYTHOSE-4-PHOSPHATE'>E4P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1u0e\|1u0e]], [[1u0f\|1u0f]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Gpi ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glucose-6-phosphate_isomerase Glucose-6-phosphate isomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.1.9 5.3.1.9] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u0g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u0g RCSB], [http://www.ebi.ac.uk/pdbsum/1u0g PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u0g_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Phosphoglucose isomerase (PGI) is an enzyme of glycolysis that interconverts glucose 6-phosphate (G6P) and fructose 6-phosphate (F6P) but, outside the cell, is a multifunctional cytokine. High-resolution crystal structures of the enzyme from mouse have been determined in native form and in complex with the inhibitor erythrose 4-phosphate, and with the substrate glucose 6-phosphate. In the substrate-bound structure, the glucose sugar is observed in both straight-chain and ring forms. This structure supports a specific role for Lys518 in enzyme-catalyzed ring opening and we present a "push-pull" mechanism in which His388 breaks the O5-C1 bond by donating a proton to the ring oxygen atom and, simultaneously, Lys518 abstracts a proton from the C1 hydroxyl group. The reverse occurs in ring closure. The transition from ring form to straight-chain substrate is achieved through rotation of the C3-C4 bond, which brings the C1-C2 region into close proximity to Glu357, the base catalyst for the isomerization step. The structure with G6P also explains the specificity of PGI for glucose 6-phosphate over mannose 6-isomerase (M6P). To isomerize M6P to F6P requires a rotation of its C2-C3 bond but in PGI this is sterically blocked by Gln511.

-	~~==About this Structure==~~	+	The crystal structure of mouse phosphoglucose isomerase at 1.6A resolution and its complex with glucose 6-phosphate reveals the catalytic mechanism of sugar ring opening.,Graham Solomons JT, Zimmerly EM, Burns S, Krishnamurthy N, Swan MK, Krings S, Muirhead H, Chirgwin J, Davies C J Mol Biol. 2004 Sep 17;342(3):847-60. PMID:15342241<ref>PMID:15342241</ref>
-	~~[[1u0g]] is a 2 chain~~ structure with ~~sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Mus_musculus Mus musculus]~~. ~~Full crystallographic information is available from [http~~://~~oca~~.~~weizmann~~.ac.il/~~oca-bin/ocashort?id=1U0G OCA].~~	+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

	==See Also==		==See Also==
	*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]		*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]
	*[[Phosphoglucose isomerase\|Phosphoglucose isomerase]]		*[[Phosphoglucose isomerase\|Phosphoglucose isomerase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015342241<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Glucose-6-phosphate isomerase]]		[[Category: Glucose-6-phosphate isomerase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]

OCA at 10:32, 11 March 2013

OCA — Mon, 11 Mar 2013 10:32:28 GMT

←Older revision		Revision as of 10:32, 11 March 2013
Line 1:		Line 1:
-	[[Image:1u0g.png\|left\|200px]]
-
	{{STRUCTURE_1u0g\| PDB=1u0g \| SCENE= }}		{{STRUCTURE_1u0g\| PDB=1u0g \| SCENE= }}
-
	===Crystal structure of mouse phosphoglucose isomerase in complex with erythrose 4-phosphate===		===Crystal structure of mouse phosphoglucose isomerase in complex with erythrose 4-phosphate===
-
	{{ABSTRACT_PUBMED_15342241}}		{{ABSTRACT_PUBMED_15342241}}

OCA at 18:51, 20 October 2012

OCA — Sat, 20 Oct 2012 18:51:35 GMT

←Older revision		Revision as of 18:51, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1u0g]] is a 2 chain structure ~~of [[Phosphoglucose isomerase]]~~ with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA].	+	[[1u0g]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA].

	==See Also==		==See Also==

OCA: Protected "1u0g" [edit=sysop:move=sysop]

OCA — Fri, 27 Jul 2012 05:29:36 GMT

Protected "1u0g" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:29, 27 July 2012

OCA at 05:29, 27 July 2012

OCA — Fri, 27 Jul 2012 05:29:33 GMT

←Older revision		Revision as of 05:29, 27 July 2012
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1u0g.png\|left\|200px]]		[[Image:1u0g.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1u0g", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1u0g\| PDB=1u0g \| SCENE= }}		{{STRUCTURE_1u0g\| PDB=1u0g \| SCENE= }}

	===Crystal structure of mouse phosphoglucose isomerase in complex with erythrose 4-phosphate===		===Crystal structure of mouse phosphoglucose isomerase in complex with erythrose 4-phosphate===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15342241}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15342241 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15342241}}		{{ABSTRACT_PUBMED_15342241}}

	==About this Structure==		==About this Structure==
-	~~1U0G~~ is a 2 ~~chains~~ structure of ~~sequences~~ from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA].	+	[[1u0g]] is a 2 chain structure of [[Phosphoglucose isomerase]] with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0G OCA].
		+
		+	==See Also==
		+	*[[Phosphoglucoisomerase\|Phosphoglucoisomerase]]
		+	*[[Phosphoglucose isomerase\|Phosphoglucose isomerase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15342241~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015342241</ref><references group="xtra"/>
	[[Category: Glucose-6-phosphate isomerase]]		[[Category: Glucose-6-phosphate isomerase]]
	[[Category: Mus musculus]]		[[Category: Mus musculus]]
Line 37:		Line 29:
	[[Category: Aldose-ketose isomerase]]		[[Category: Aldose-ketose isomerase]]
	[[Category: Dimer]]		[[Category: Dimer]]
-		+	[[Category: Isomerase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 21:39:12 2009''~~	+