1u6z - Revision history

OCA at 08:44, 14 February 2024

OCA — Wed, 14 Feb 2024 08:44:21 GMT

←Older revision		Revision as of 08:44, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1u6z' size='340' side='right'caption='[[1u6z]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1u6z' size='340' side='right'caption='[[1u6z]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1u6z]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1U6Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1u6z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U6Z FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>ppx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~562~~ "~~Bacillus coli~~" ~~Migula 1895])</td~~><~~/tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b></td><td class="sblockDat"><span class='plainlinks~~'>~~[http://en.wikipedia.org/wiki/Exopolyphosphatase Exopolyphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.11 3.6.1.11]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u6z OCA], [https://pdbe.org/1u6z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u6z RCSB], [https://www.ebi.ac.uk/pdbsum/1u6z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u6z ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1u6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u6z OCA], [~~http~~://pdbe.org/1u6z PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1u6z RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1u6z PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1u6z ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/PPX_ECOLI PPX_ECOLI]] Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate.	+	[https://www.uniprot.org/uniprot/PPX_ECOLI PPX_ECOLI] Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 21:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u6z ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u6z ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The Escherichia coli Ppx protein is an exopolyphosphatase that degrades long-chain polyphosphates in a highly processive reaction. It also hydrolyzes the terminal 5' phosphate of the modified nucleotide guanosine 5' triphosphate 3' diphosphate (pppGpp). The structure of Ppx has been determined to 1.9 A resolution by X-ray crystallography. The exopolyphosphatase is an ASKHA (acetate and sugar kinases, Hsp70, actin) phosphotransferase with an active site found in a cleft between the two amino-terminal domains. Analysis of the active site indicates that among the ASKHA phosphotranferases of known structure, Ppx is the closest to the ectonucleoside triphosphate diphosphohydrolases. A third domain forms a six-helix claw that is similar to the catalytic core of the eukaryotic cyclic nucleotide phosphodiesterases. Most of the 29 sulfate ions bound to the Ppx dimer occupy sites where the polyP chain likely binds. An aqueduct that passes through the enzyme provides a physical basis for the enzyme's high processivity.
-
-	Origin of exopolyphosphatase processivity: Fusion of an ASKHA phosphotransferase and a cyclic nucleotide phosphodiesterase homolog.,Alvarado J, Ghosh A, Janovitz T, Jauregui A, Hasson MS, Sanders DA Structure. 2006 Aug;14(8):1263-72. PMID:16905100<ref>PMID:16905100</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1u6z" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: Exopolyphosphatase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Alvarado, J]]	+	[[Category: Alvarado J]]
-	[[Category: Hasson~~, M S~~]]	+	[[Category: Hasson MS]]
-	[[Category: Sanders~~, D A]]~~	+	[[Category: Sanders DA]]
-	~~[[Category: Alpha/beta protein]]~~	+
-	~~[[Category: Hd metal dependent phosphohydrolase superfamily]]~~	+
-	~~[[Category: Hsc70]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Polyphosphate]]~~	+
-	~~[[Category: Twenty-nine sulfate~~]]	+

OCA at 07:23, 30 December 2020

OCA — Wed, 30 Dec 2020 07:23:30 GMT

←Older revision		Revision as of 07:23, 30 December 2020
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	==Structure of an E. coli Exopolyphosphatase: Insight into the processive hydrolysis of polyphosphate and its regulation==		==Structure of an E. coli Exopolyphosphatase: Insight into the processive hydrolysis of polyphosphate and its regulation==
-	<StructureSection load='1u6z' size='340' side='right' caption='[[1u6z]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1u6z' size='340' side='right'caption='[[1u6z]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1u6z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1U6Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1u6z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1U6Z FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ppx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ppx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exopolyphosphatase Exopolyphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.11 3.6.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exopolyphosphatase Exopolyphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.11 3.6.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1u6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u6z OCA], [http://pdbe.org/1u6z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u6z RCSB], [http://www.ebi.ac.uk/pdbsum/1u6z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u6z ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1u6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u6z OCA], [http://pdbe.org/1u6z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u6z RCSB], [http://www.ebi.ac.uk/pdbsum/1u6z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u6z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 36:		Line 36:
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
	[[Category: Exopolyphosphatase]]		[[Category: Exopolyphosphatase]]
		+	[[Category: Large Structures]]
	[[Category: Alvarado, J]]		[[Category: Alvarado, J]]
	[[Category: Hasson, M S]]		[[Category: Hasson, M S]]

OCA at 08:31, 21 March 2018

OCA — Wed, 21 Mar 2018 08:31:43 GMT

←Older revision		Revision as of 08:31, 21 March 2018
Line 1:		Line 1:
		+
	==Structure of an E. coli Exopolyphosphatase: Insight into the processive hydrolysis of polyphosphate and its regulation==		==Structure of an E. coli Exopolyphosphatase: Insight into the processive hydrolysis of polyphosphate and its regulation==
	<StructureSection load='1u6z' size='340' side='right' caption='[[1u6z]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1u6z' size='340' side='right' caption='[[1u6z]], [[Resolution\|resolution]] 1.90Å' scene=''>
Line 6:		Line 7:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ppx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ppx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exopolyphosphatase Exopolyphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.11 3.6.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exopolyphosphatase Exopolyphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.11 3.6.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u6z OCA], [http://pdbe.org/1u6z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u6z RCSB], [http://www.ebi.ac.uk/pdbsum/1u6z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u6z OCA], [http://pdbe.org/1u6z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u6z RCSB], [http://www.ebi.ac.uk/pdbsum/1u6z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u6z ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u6/1u6z_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u6/1u6z_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u6z ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 11:59, 11 September 2015

OCA — Fri, 11 Sep 2015 11:59:28 GMT

←Older revision		Revision as of 11:59, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1u6z' size='340' side='right' caption='[[1u6z]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1u6z' size='340' side='right' caption='[[1u6z]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1u6z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U6Z FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1u6z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U6Z FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ppx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 ~~Escherichia~~ coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ppx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exopolyphosphatase Exopolyphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.11 3.6.1.11] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exopolyphosphatase Exopolyphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.11 3.6.1.11] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u6z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u6z RCSB], [http://www.ebi.ac.uk/pdbsum/1u6z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u6z OCA], [http://pdbe.org/1u6z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u6z RCSB], [http://www.ebi.ac.uk/pdbsum/1u6z PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1u6z" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Exopolyphosphatase]]		[[Category: Exopolyphosphatase]]
	[[Category: Alvarado, J]]		[[Category: Alvarado, J]]

OCA at 16:09, 25 December 2014

OCA — Thu, 25 Dec 2014 16:09:44 GMT

←Older revision		Revision as of 16:09, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1u6z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U6Z FirstGlance]. <br>		<table><tr><td colspan='2'>[[1u6z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U6Z FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ppx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ppx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exopolyphosphatase Exopolyphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.11 3.6.1.11] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exopolyphosphatase Exopolyphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.11 3.6.1.11] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u6z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u6z RCSB], [http://www.ebi.ac.uk/pdbsum/1u6z PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u6z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u6z RCSB], [http://www.ebi.ac.uk/pdbsum/1u6z PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/PPX_ECOLI PPX_ECOLI]] Degradation of inorganic polyphosphates. Orthophosphate is released progressively from the ends of polyphosphate of circa 500 residues long, while chains of circa 15 residues compete poorly with polyphosphate as substrate.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 32:		Line 34:
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Exopolyphosphatase]]		[[Category: Exopolyphosphatase]]
-	[[Category: Alvarado, J.]]	+	[[Category: Alvarado, J]]
-	[[Category: Hasson, M S.]]	+	[[Category: Hasson, M S]]
-	[[Category: Sanders, D A.]]	+	[[Category: Sanders, D A]]
	[[Category: Alpha/beta protein]]		[[Category: Alpha/beta protein]]
	[[Category: Hd metal dependent phosphohydrolase superfamily]]		[[Category: Hd metal dependent phosphohydrolase superfamily]]

OCA at 19:26, 29 September 2014

OCA — Mon, 29 Sep 2014 19:26:37 GMT

←Older revision		Revision as of 19:26, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1u6z.~~png~~\|~~left~~\|200px]]	+	==Structure of an E. coli Exopolyphosphatase: Insight into the processive hydrolysis of polyphosphate and its regulation==
		+	<StructureSection load='1u6z' size='340' side='right' caption='[[1u6z]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1u6z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1U6Z FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">ppx ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exopolyphosphatase Exopolyphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.11 3.6.1.11] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u6z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u6z OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1u6z RCSB], [http://www.ebi.ac.uk/pdbsum/1u6z PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u6/1u6z_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Escherichia coli Ppx protein is an exopolyphosphatase that degrades long-chain polyphosphates in a highly processive reaction. It also hydrolyzes the terminal 5' phosphate of the modified nucleotide guanosine 5' triphosphate 3' diphosphate (pppGpp). The structure of Ppx has been determined to 1.9 A resolution by X-ray crystallography. The exopolyphosphatase is an ASKHA (acetate and sugar kinases, Hsp70, actin) phosphotransferase with an active site found in a cleft between the two amino-terminal domains. Analysis of the active site indicates that among the ASKHA phosphotranferases of known structure, Ppx is the closest to the ectonucleoside triphosphate diphosphohydrolases. A third domain forms a six-helix claw that is similar to the catalytic core of the eukaryotic cyclic nucleotide phosphodiesterases. Most of the 29 sulfate ions bound to the Ppx dimer occupy sites where the polyP chain likely binds. An aqueduct that passes through the enzyme provides a physical basis for the enzyme's high processivity.

-	~~{{STRUCTURE_1u6z\| PDB=1u6z \| SCENE= }}~~	+	Origin of exopolyphosphatase processivity: Fusion of an ASKHA phosphotransferase and a cyclic nucleotide phosphodiesterase homolog.,Alvarado J, Ghosh A, Janovitz T, Jauregui A, Hasson MS, Sanders DA Structure. 2006 Aug;14(8):1263-72. PMID:16905100<ref>PMID:16905100</ref>

-	~~===Structure~~ of ~~an E. coli Exopolyphosphatase: Insight into~~ the ~~processive hydrolysis of polyphosphate and its regulation===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16905100}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1u6z]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:016905100<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Exopolyphosphatase]]		[[Category: Exopolyphosphatase]]

OCA: Protected "1u6z" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 12:31:32 GMT

Protected "1u6z" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:31, 5 January 2013

OCA at 12:31, 5 January 2013

OCA — Sat, 05 Jan 2013 12:31:28 GMT

←Older revision		Revision as of 12:31, 5 January 2013
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-	{{Seed}}
	[[Image:1u6z.png\|left\|200px]]		[[Image:1u6z.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1u6z", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1u6z\| PDB=1u6z \| SCENE= }}		{{STRUCTURE_1u6z\| PDB=1u6z \| SCENE= }}

	===Structure of an E. coli Exopolyphosphatase: Insight into the processive hydrolysis of polyphosphate and its regulation===		===Structure of an E. coli Exopolyphosphatase: Insight into the processive hydrolysis of polyphosphate and its regulation===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_16905100}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 16905100 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_16905100}}		{{ABSTRACT_PUBMED_16905100}}

	==About this Structure==		==About this Structure==
-	~~1U6Z~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA].	+	[[1u6z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~16905100~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:016905100</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Exopolyphosphatase]]		[[Category: Exopolyphosphatase]]
Line 32:		Line 20:
	[[Category: Hd metal dependent phosphohydrolase superfamily]]		[[Category: Hd metal dependent phosphohydrolase superfamily]]
	[[Category: Hsc70]]		[[Category: Hsc70]]
		+	[[Category: Hydrolase]]
	[[Category: Polyphosphate]]		[[Category: Polyphosphate]]
	[[Category: Twenty-nine sulfate]]		[[Category: Twenty-nine sulfate]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:22:03 2009''

OCA at 19:22, 17 February 2009

OCA — Tue, 17 Feb 2009 19:22:05 GMT

←Older revision		Revision as of 19:22, 17 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1U6Z is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA].	+	1U6Z is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA].

	==Reference==		==Reference==
-	Origin of exopolyphosphatase processivity: Fusion of an ASKHA phosphotransferase and a cyclic nucleotide phosphodiesterase homolog., Alvarado J, Ghosh A, Janovitz T, Jauregui A, Hasson MS, Sanders DA, Structure. 2006 Aug;14(8):1263-72. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/16905100 16905100]~~	+	<ref group="xtra">PMID:16905100</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Exopolyphosphatase]]		[[Category: Exopolyphosphatase]]
-	[[Category: Single protein]]
	[[Category: Alvarado, J.]]		[[Category: Alvarado, J.]]
	[[Category: Hasson, M S.]]		[[Category: Hasson, M S.]]
Line 36:		Line 35:
	[[Category: Twenty-nine sulfate]]		[[Category: Twenty-nine sulfate]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Wed Jul 16 08~~:54:~~09 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 21:22:03 2009''

OCA at 05:54, 16 July 2008

OCA — Wed, 16 Jul 2008 05:54:10 GMT

←Older revision		Revision as of 05:54, 16 July 2008
Line 1:		Line 1:
-	[[Image:1u6z.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1u6z.png\|left\|200px]]

	<!--		<!--
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	{{STRUCTURE_1u6z\| PDB=1u6z \| SCENE= }}		{{STRUCTURE_1u6z\| PDB=1u6z \| SCENE= }}

-	~~'''~~Structure of an E. coli Exopolyphosphatase: Insight into the processive hydrolysis of polyphosphate and its regulation~~'''~~	+	===Structure of an E. coli Exopolyphosphatase: Insight into the processive hydrolysis of polyphosphate and its regulation===

		+
		+	<!--
		+	The line below this paragraph, {{ABSTRACT_PUBMED_16905100}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 16905100 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_16905100}}

	==About this Structure==		==About this Structure==
	1U6Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA].		1U6Z is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U6Z OCA].
		+
		+	==Reference==
		+	Origin of exopolyphosphatase processivity: Fusion of an ASKHA phosphotransferase and a cyclic nucleotide phosphodiesterase homolog., Alvarado J, Ghosh A, Janovitz T, Jauregui A, Hasson MS, Sanders DA, Structure. 2006 Aug;14(8):1263-72. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16905100 16905100]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Exopolyphosphatase]]		[[Category: Exopolyphosphatase]]
Line 25:		Line 35:
	[[Category: Polyphosphate]]		[[Category: Polyphosphate]]
	[[Category: Twenty-nine sulfate]]		[[Category: Twenty-nine sulfate]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 10~~:50:02 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 16 08:54:09 2008''