1ucc - Revision history

OCA at 07:33, 25 October 2023

OCA — Wed, 25 Oct 2023 07:33:49 GMT

←Older revision		Revision as of 07:33, 25 October 2023
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ucc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UCC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ucc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UCC FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=U3P:3~~-~~URIDINEMONOPHOSPHATE'>U3P</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.77Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[1bk7\|1bk7]], [[1uca\|1uca]]</div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=U3P:3-URIDINEMONOPHOSPHATE'>U3P</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity:</b></td><td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ucc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucc OCA], [https://pdbe.org/1ucc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ucc RCSB], [https://www.ebi.ac.uk/pdbsum/1ucc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ucc ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ucc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucc OCA], [https://pdbe.org/1ucc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ucc RCSB], [https://www.ebi.ac.uk/pdbsum/1ucc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ucc ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/RNMC1_MOMCH RNMC1_MOMCH] Ribonuclease cleaving preferentially the 5'-side of uridine.<ref>PMID:10964705</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
-	[[Category: Kikukawa, S]]	+	[[Category: Kikukawa S]]
-	[[Category: Kimura, M]]	+	[[Category: Kimura M]]
-	[[Category: Numata, T]]	+	[[Category: Numata T]]
-	[[Category: Suzuki, A]]	+	[[Category: Suzuki A]]
-	[[Category: Tanaka, I]]	+	[[Category: Tanaka I]]
-	[[Category: Yamasaki, N]]	+	[[Category: Yamasaki N]]
-	[[Category: Yao, M]]	+	[[Category: Yao M]]
-	~~[[Category: Alpha plus beta]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 09:39, 29 September 2021

OCA — Wed, 29 Sep 2021 09:39:57 GMT

←Older revision		Revision as of 09:39, 29 September 2021
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	<StructureSection load='1ucc' size='340' side='right'caption='[[1ucc]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='1ucc' size='340' side='right'caption='[[1ucc]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1ucc]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1UCC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1ucc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UCC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=U3P:3-URIDINEMONOPHOSPHATE'>U3P</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=U3P:3-URIDINEMONOPHOSPHATE'>U3P</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1bk7\|1bk7]], [[1uca\|1uca]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1ucc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucc OCA], [~~http~~://pdbe.org/1ucc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1ucc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1ucc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1ucc ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ucc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucc OCA], [https://pdbe.org/1ucc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ucc RCSB], [https://www.ebi.ac.uk/pdbsum/1ucc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ucc ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]		*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
-	*[[Temp\|Temp]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 14:10, 25 December 2019

OCA — Wed, 25 Dec 2019 14:10:18 GMT

←Older revision		Revision as of 14:10, 25 December 2019
Line 1:		Line 1:

	==Crystal structure of the Ribonuclease MC1 from bitter gourd seeds complexed with 3'-UMP.==		==Crystal structure of the Ribonuclease MC1 from bitter gourd seeds complexed with 3'-UMP.==
-	<StructureSection load='1ucc' size='340' side='right' caption='[[1ucc]], [[Resolution\|resolution]] 1.77Å' scene=''>	+	<StructureSection load='1ucc' size='340' side='right'caption='[[1ucc]], [[Resolution\|resolution]] 1.77Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ucc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UCC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ucc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UCC FirstGlance]. <br>
Line 30:		Line 30:

	==See Also==		==See Also==
-	*[[Ribonuclease\|Ribonuclease]]	+	*[[Ribonuclease 3D structures\|Ribonuclease 3D structures]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
	== References ==		== References ==
Line 36:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
	[[Category: Kikukawa, S]]		[[Category: Kikukawa, S]]

OCA at 08:59, 21 March 2018

OCA — Wed, 21 Mar 2018 08:59:11 GMT

←Older revision		Revision as of 08:59, 21 March 2018
Line 1:		Line 1:
		+
	==Crystal structure of the Ribonuclease MC1 from bitter gourd seeds complexed with 3'-UMP.==		==Crystal structure of the Ribonuclease MC1 from bitter gourd seeds complexed with 3'-UMP.==
	<StructureSection load='1ucc' size='340' side='right' caption='[[1ucc]], [[Resolution\|resolution]] 1.77Å' scene=''>		<StructureSection load='1ucc' size='340' side='right' caption='[[1ucc]], [[Resolution\|resolution]] 1.77Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucc OCA], [http://pdbe.org/1ucc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ucc RCSB], [http://www.ebi.ac.uk/pdbsum/1ucc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucc OCA], [http://pdbe.org/1ucc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ucc RCSB], [http://www.ebi.ac.uk/pdbsum/1ucc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ucc ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uc/1ucc_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uc/1ucc_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 22:29, 8 February 2016

OCA — Mon, 08 Feb 2016 22:29:11 GMT

←Older revision		Revision as of 22:29, 8 February 2016
Line 16:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1ucc ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 15:58, 10 September 2015

OCA — Thu, 10 Sep 2015 15:58:00 GMT

←Older revision		Revision as of 15:58, 10 September 2015
Line 6:		Line 6:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ucc RCSB], [http://www.ebi.ac.uk/pdbsum/1ucc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucc OCA], [http://pdbe.org/1ucc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ucc RCSB], [http://www.ebi.ac.uk/pdbsum/1ucc PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1ucc" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
	*[[Temp\|Temp]]		*[[Temp\|Temp]]
-	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:59, 6 January 2015

OCA — Tue, 06 Jan 2015 13:59:45 GMT

←Older revision		Revision as of 13:59, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1ucc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UCC FirstGlance]. <br>		<table><tr><td colspan='2'>[[1ucc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UCC FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=U3P:3-URIDINEMONOPHOSPHATE'>U3P</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=U3P:3-URIDINEMONOPHOSPHATE'>U3P</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ucc RCSB], [http://www.ebi.ac.uk/pdbsum/1ucc PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ucc RCSB], [http://www.ebi.ac.uk/pdbsum/1ucc PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
		+	*[[Temp\|Temp]]
		+	*[[User:Jaime.Prilusky/Test/tree\|User:Jaime.Prilusky/Test/tree]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
-	[[Category: Kikukawa, S.]]	+	[[Category: Kikukawa, S]]
-	[[Category: Kimura, M.]]	+	[[Category: Kimura, M]]
-	[[Category: Numata, T.]]	+	[[Category: Numata, T]]
-	[[Category: Suzuki, A.]]	+	[[Category: Suzuki, A]]
-	[[Category: Tanaka, I.]]	+	[[Category: Tanaka, I]]
-	[[Category: Yamasaki, N.]]	+	[[Category: Yamasaki, N]]
-	[[Category: Yao, M.]]	+	[[Category: Yao, M]]
	[[Category: Alpha plus beta]]		[[Category: Alpha plus beta]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 00:10, 29 September 2014

OCA — Mon, 29 Sep 2014 00:10:03 GMT

←Older revision		Revision as of 00:10, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1ucc.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of the Ribonuclease MC1 from bitter gourd seeds complexed with 3'-UMP.==
		+	<StructureSection load='1ucc' size='340' side='right' caption='[[1ucc]], [[Resolution\|resolution]] 1.77Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1ucc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UCC FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=U3P:3-URIDINEMONOPHOSPHATE'>U3P</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1bk7\|1bk7]], [[1uca\|1uca]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_T(2) Ribonuclease T(2)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.1 3.1.27.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ucc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ucc OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1ucc RCSB], [http://www.ebi.ac.uk/pdbsum/1ucc PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/uc/1ucc_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Ribonuclease MC1 (RNase MC1) isolated from seeds of bitter gourd (Momordica charantia) consists of 190 amino acids and is characterized by a preferential cleavage at the 5'-side of uridine. This uridine specificity distinguishes RNase MC1 from other enzymes belonging to the RNase T2 family. The three-dimensional structures of RNase MC1, in a complex with either 2'-UMP or 3'-UMP, were determined at 1.48 and 1.77 A resolutions, respectively. The side chains of Gln9 and Asn71 interact with O4 and N3, respectively, of the uracil base by hydrogen bondings. In addition, the uracil base is sandwiched by the hydrophobic side chains of Leu73 and Phe80. Compared with these amino acid residues and corresponding residues in RNases in the RNase T2 family, Gln9 and Phe80 are highly conserved in the RNases in T2 family, while Asn71 and Leu73 in RNase MC1 are variant in sequences. It is thus likely that interactions of the side chains of Asn71 and Leu73 with the uracil base are responsible for the absolute uridine specificity of RNase MC1. Site-directed mutagenesis experiments showed that replacement of Asn by Thr decreased both the catalytic efficiency and the binding affinity by 2.3- and 7.0-fold, respectively, and substitution of Leu73 for Ala predominantly decreased the binding affinity by 14. 5-fold, compared with findings in case of wild-type RNase MC1. It is thus demonstrated that Asn71 and Leu73 play an essential role in uridine preference for RNase MC1.

-	~~{{STRUCTURE_1ucc\| PDB=1ucc \| SCENE= }}~~	+	Crystal structures of the ribonuclease MC1 from bitter gourd seeds, complexed with 2'-UMP or 3'-UMP, reveal structural basis for uridine specificity.,Suzuki A, Yao M, Tanaka I, Numata T, Kikukawa S, Yamasaki N, Kimura M Biochem Biophys Res Commun. 2000 Aug 28;275(2):572-6. PMID:10964705<ref>PMID:10964705</ref>

-	~~===Crystal structure~~ of the ~~Ribonuclease MC1 from bitter gourd seeds complexed with 3'-UMP~~.~~===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_10964705}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1ucc]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCC OCA].~~	+

	==See Also==		==See Also==
	*[[Ribonuclease\|Ribonuclease]]		*[[Ribonuclease\|Ribonuclease]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:010964705<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Momordica charantia]]		[[Category: Momordica charantia]]
	[[Category: Kikukawa, S.]]		[[Category: Kikukawa, S.]]

OCA at 11:41, 20 October 2012

OCA — Sat, 20 Oct 2012 11:41:07 GMT

←Older revision		Revision as of 11:41, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1ucc]] is a 1 chain structure ~~of [[Ribonuclease]]~~ with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCC OCA].	+	[[1ucc]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Momordica_charantia Momordica charantia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UCC OCA].

	==See Also==		==See Also==

OCA: Protected "1ucc" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 06:15:13 GMT

Protected "1ucc" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:15, 26 July 2012