1uxg - Revision history

OCA at 08:06, 15 November 2023

OCA — Wed, 15 Nov 2023 08:06:56 GMT

←Older revision		Revision as of 08:06, 15 November 2023
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	<StructureSection load='1uxg' size='340' side='right'caption='[[1uxg]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1uxg' size='340' side='right'caption='[[1uxg]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1uxg]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Atcc_29366 Atcc 29366~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1UXG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1uxg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Chloroflexus_aurantiacus Chloroflexus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UXG FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>~~, ~~<scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[1guy\|1guy]], [[1ur5\|1ur5]], [[1uxh\|1uxh]], [[1uxi\|1uxi]], [[1uxj\|1uxj]], [[1uxk\|1uxk]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-	~~<tr~~ id~~='activity'><td class~~="~~sblockLbl~~"><~~b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37]~~ </~~span~~></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxg OCA], [https://pdbe.org/1uxg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uxg RCSB], [https://www.ebi.ac.uk/pdbsum/1uxg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uxg ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1uxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxg OCA], [~~http~~://pdbe.org/1uxg PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1uxg RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1uxg PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1uxg ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MDH_CHLAA MDH_CHLAA]] Catalyzes the reversible oxidation of malate to oxaloacetate.[HAMAP-Rule:MF_00487]	+	[https://www.uniprot.org/uniprot/MDH_CHLAA MDH_CHLAA] Catalyzes the reversible oxidation of malate to oxaloacetate.[HAMAP-Rule:MF_00487]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Atcc 29366~~]]	+	[[Category: Chloroflexus aurantiacus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Malate dehydrogenase]]~~	+	[[Category: Bjork A]]
-	[[Category: Bjork, A]]	+	[[Category: Dalhus B]]
-	[[Category: Dalhus, B]]	+	[[Category: Eijsink VGH]]
-	[[Category: Eijsink~~, V G.H~~]]	+	[[Category: Mantzilas D]]
-	[[Category: Mantzilas, D]]	+	[[Category: Sirevag R]]
-	[[Category: Sirevag, R]]	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Tricarboxylic acid cycle~~]]	+

OCA at 11:49, 29 July 2020

OCA — Wed, 29 Jul 2020 11:49:35 GMT

←Older revision		Revision as of 11:49, 29 July 2020
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	==Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface.==		==Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface.==
-	<StructureSection load='1uxg' size='340' side='right' caption='[[1uxg]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1uxg' size='340' side='right'caption='[[1uxg]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1uxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29366 Atcc 29366]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1UXG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1uxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29366 Atcc 29366]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1UXG FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1guy\|1guy]], [[1ur5\|1ur5]], [[1uxh\|1uxh]], [[1uxi\|1uxi]], [[1uxj\|1uxj]], [[1uxk\|1uxk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1guy\|1guy]], [[1ur5\|1ur5]], [[1uxh\|1uxh]], [[1uxi\|1uxi]], [[1uxj\|1uxj]], [[1uxk\|1uxk]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1uxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxg OCA], [http://pdbe.org/1uxg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uxg RCSB], [http://www.ebi.ac.uk/pdbsum/1uxg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1uxg ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1uxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxg OCA], [http://pdbe.org/1uxg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uxg RCSB], [http://www.ebi.ac.uk/pdbsum/1uxg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1uxg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Malate ~~dehydrogenase~~\|Malate ~~dehydrogenase~~]]	+	*[[Malate Dehydrogenase 3D structures\|Malate Dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 29366]]		[[Category: Atcc 29366]]
		+	[[Category: Large Structures]]
	[[Category: Malate dehydrogenase]]		[[Category: Malate dehydrogenase]]
	[[Category: Bjork, A]]		[[Category: Bjork, A]]

OCA at 07:35, 24 October 2018

OCA — Wed, 24 Oct 2018 07:35:10 GMT

←Older revision		Revision as of 07:35, 24 October 2018
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ux/1uxg_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ux/1uxg_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 17:25, 18 January 2017

OCA — Wed, 18 Jan 2017 17:25:03 GMT

←Older revision		Revision as of 17:25, 18 January 2017
Line 1:		Line 1:
		+
	==Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface.==		==Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface.==
	<StructureSection load='1uxg' size='340' side='right' caption='[[1uxg]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1uxg' size='340' side='right' caption='[[1uxg]], [[Resolution\|resolution]] 1.90Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1guy\|1guy]], [[1ur5\|1ur5]], [[1uxh\|1uxh]], [[1uxi\|1uxi]], [[1uxj\|1uxj]], [[1uxk\|1uxk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1guy\|1guy]], [[1ur5\|1ur5]], [[1uxh\|1uxh]], [[1uxi\|1uxi]], [[1uxj\|1uxj]], [[1uxk\|1uxk]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uxg RCSB], [http://www.ebi.ac.uk/pdbsum/1uxg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxg OCA], [http://pdbe.org/1uxg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uxg RCSB], [http://www.ebi.ac.uk/pdbsum/1uxg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1uxg ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 18:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uxg ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1uxg" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 06:43, 15 April 2015

OCA — Wed, 15 Apr 2015 06:43:47 GMT

←Older revision		Revision as of 06:43, 15 April 2015
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	<StructureSection load='1uxg' size='340' side='right' caption='[[1uxg]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1uxg' size='340' side='right' caption='[[1uxg]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1uxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Chloroflexus_aurantiacus Chloroflexus aurantiacus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UXG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1uxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_29366 Atcc 29366]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UXG FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1guy\|1guy]], [[1ur5\|1ur5]], [[1uxh\|1uxh]], [[1uxi\|1uxi]], [[1uxj\|1uxj]], [[1uxk\|1uxk]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1guy\|1guy]], [[1ur5\|1ur5]], [[1uxh\|1uxh]], [[1uxi\|1uxi]], [[1uxj\|1uxj]], [[1uxk\|1uxk]]</td></tr>
Line 9:		Line 9:
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~MDH_CHLAU MDH_CHLAU~~]] Catalyzes the reversible oxidation of malate to oxaloacetate.[HAMAP-Rule:MF_00487]	+	[[http://www.uniprot.org/uniprot/MDH_CHLAA MDH_CHLAA]] Catalyzes the reversible oxidation of malate to oxaloacetate.[HAMAP-Rule:MF_00487]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chloroflexus aurantiacus~~]]	+	[[Category: Atcc 29366]]
	[[Category: Malate dehydrogenase]]		[[Category: Malate dehydrogenase]]
	[[Category: Bjork, A]]		[[Category: Bjork, A]]

OCA at 21:27, 25 December 2014

OCA — Thu, 25 Dec 2014 21:27:01 GMT

←Older revision		Revision as of 21:27, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1uxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chloroflexus_aurantiacus Chloroflexus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UXG FirstGlance]. <br>		<table><tr><td colspan='2'>[[1uxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chloroflexus_aurantiacus Chloroflexus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UXG FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1guy\|1guy]], [[1ur5\|1ur5]], [[1uxh\|1uxh]], [[1uxi\|1uxi]], [[1uxj\|1uxj]], [[1uxk\|1uxk]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1guy\|1guy]], [[1ur5\|1ur5]], [[1uxh\|1uxh]], [[1uxi\|1uxi]], [[1uxj\|1uxj]], [[1uxk\|1uxk]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uxg RCSB], [http://www.ebi.ac.uk/pdbsum/1uxg PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uxg RCSB], [http://www.ebi.ac.uk/pdbsum/1uxg PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MDH_CHLAU MDH_CHLAU]] Catalyzes the reversible oxidation of malate to oxaloacetate.[HAMAP-Rule:MF_00487]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Chloroflexus aurantiacus]]		[[Category: Chloroflexus aurantiacus]]
	[[Category: Malate dehydrogenase]]		[[Category: Malate dehydrogenase]]
-	[[Category: Bjork, A.]]	+	[[Category: Bjork, A]]
-	[[Category: Dalhus, B.]]	+	[[Category: Dalhus, B]]
-	[[Category: Eijsink, V G.H.]]	+	[[Category: Eijsink, V G.H]]
-	[[Category: Mantzilas, D.]]	+	[[Category: Mantzilas, D]]
-	[[Category: Sirevag, R~~.]]~~	+	[[Category: Sirevag, R]]
-	~~[[Category: Malate dehydrogenase~~]]	+
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Tricarboxylic acid cycle]]		[[Category: Tricarboxylic acid cycle]]

OCA at 05:18, 3 October 2014

OCA — Fri, 03 Oct 2014 05:18:36 GMT

←Older revision		Revision as of 05:18, 3 October 2014
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-	[[~~Image:~~1uxg.~~png~~\|~~left~~\|200px]]	+	==Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface.==
		+	<StructureSection load='1uxg' size='340' side='right' caption='[[1uxg]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1uxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chloroflexus_aurantiacus Chloroflexus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UXG FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FUM:FUMARIC+ACID'>FUM</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1guy\|1guy]], [[1ur5\|1ur5]], [[1uxh\|1uxh]], [[1uxi\|1uxi]], [[1uxj\|1uxj]], [[1uxk\|1uxk]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Malate_dehydrogenase Malate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.37 1.1.1.37] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxg OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uxg RCSB], [http://www.ebi.ac.uk/pdbsum/1uxg PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ux/1uxg_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The stability of tetrameric malate dehydrogenase from the green phototrophic bacterium Chloroflexus aurantiacus (CaMDH) is at least in part determined by electrostatic interactions at the dimer-dimer interface. Since previous studies had indicated that the thermal stability of CaMDH becomes lower with increasing pH, attempts were made to increase the stability by removal of (excess) negative charge at the dimer-dimer interface. Mutation of Glu165 to Gln or Lys yielded a dramatic increase in thermal stability at pH 7.5 (+23.6 -- + 23.9 degrees C increase in apparent t(m)) and a more moderate increase at pH 4.4 (+4.6 -- + 5.4 degrees C). The drastically increased stability at neutral pH was achieved without forfeiture of catalytic performance at low temperatures. The crystal structures of the two mutants showed only minor structural changes close to the mutated residues, and indicated that the observed stability effects are solely due to subtle changes in the complex network of electrostatic interactions in the dimer-dimer interface. Both mutations reduced the concentration dependency of thermal stability, suggesting that the oligomeric structure had been reinforced. Interestingly, the two mutations had similar effects on stability, despite the charge difference between the introduced side-chains. Together with the loss of concentration dependency, this may indicate that both E165Q and E165K stabilize CaMDH to such an extent that disruption of the inter-dimer electrostatic network around residue 165 no longer limits kinetic thermal stability.

-	~~{{STRUCTURE_1uxg\| PDB=1uxg \| SCENE= }}~~	+	Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface.,Bjork A, Dalhus B, Mantzilas D, Sirevag R, Eijsink VG J Mol Biol. 2004 Aug 27;341(5):1215-26. PMID:15321717<ref>PMID:15321717</ref>

-	~~===Large improvement in the thermal stability~~ of ~~a tetrameric malate dehydrogenase by single point mutations at~~ the ~~dimer-dimer interface~~.~~===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15321717}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1uxg]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Chloroflexus_aurantiacus Chloroflexus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA].~~	+

	==See Also==		==See Also==
-	*[[Johnson sandbox 1\|Johnson sandbox 1]]
	*[[Malate dehydrogenase\|Malate dehydrogenase]]		*[[Malate dehydrogenase\|Malate dehydrogenase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015321717<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Chloroflexus aurantiacus]]		[[Category: Chloroflexus aurantiacus]]
	[[Category: Malate dehydrogenase]]		[[Category: Malate dehydrogenase]]

OCA at 21:51, 20 October 2012

OCA — Sat, 20 Oct 2012 21:51:26 GMT

←Older revision		Revision as of 21:51, 20 October 2012
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	==About this Structure==		==About this Structure==
-	[[1uxg]] is a 2 chain structure ~~of [[Johnson sandbox 1]] and [[Malate dehydrogenase]]~~ with sequence from [http://en.wikipedia.org/wiki/Chloroflexus_aurantiacus Chloroflexus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA].	+	[[1uxg]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Chloroflexus_aurantiacus Chloroflexus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA].

	==See Also==		==See Also==

OCA at 04:41, 18 July 2012

OCA — Wed, 18 Jul 2012 04:41:57 GMT

←Older revision		Revision as of 04:41, 18 July 2012
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	[[Image:1uxg.png\|left\|200px]]		[[Image:1uxg.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1uxg\| PDB=1uxg \| SCENE= }}		{{STRUCTURE_1uxg\| PDB=1uxg \| SCENE= }}

-	===~~LARGE IMPROVEMENT IN THE THERMAL STABILITY OF A TETRAMERIC MALATE DEHYDROGENASE BY SINGLE POINT MUTATIONS AT THE DIMER~~-~~DIMER INTERFACE~~.===	+	===Large improvement in the thermal stability of a tetrameric malate dehydrogenase by single point mutations at the dimer-dimer interface.===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15321717}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15321717 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_15321717}}		{{ABSTRACT_PUBMED_15321717}}

	==About this Structure==		==About this Structure==
-	[[1uxg]] is a 2 chain structure of [[Malate dehydrogenase]] with sequence from [http://en.wikipedia.org/wiki/Chloroflexus_aurantiacus Chloroflexus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA].	+	[[1uxg]] is a 2 chain structure of [[Johnson sandbox 1]] and [[Malate dehydrogenase]] with sequence from [http://en.wikipedia.org/wiki/Chloroflexus_aurantiacus Chloroflexus aurantiacus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXG OCA].

	==See Also==		==See Also==
-	*[[Malate dehydrogenase]]	+	*[[Johnson sandbox 1\|Johnson sandbox 1]]
		+	*[[Malate dehydrogenase\|Malate dehydrogenase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15321717~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015321717</ref><references group="xtra"/>
	[[Category: Chloroflexus aurantiacus]]		[[Category: Chloroflexus aurantiacus]]
	[[Category: Malate dehydrogenase]]		[[Category: Malate dehydrogenase]]

OCA: Protected "1uxg" [edit=sysop:move=sysop]

OCA — Mon, 27 Dec 2010 01:09:44 GMT

Protected "1uxg" [edit=sysop:move=sysop]

←Older revision

Revision as of 01:09, 27 December 2010