1uxt - Revision history

OCA at 13:03, 13 December 2023

OCA — Wed, 13 Dec 2023 13:03:10 GMT

←Older revision		Revision as of 13:03, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='1uxt' size='340' side='right'caption='[[1uxt]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1uxt' size='340' side='right'caption='[[1uxt]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1uxt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_35583 Atcc 35583~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UXT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1uxt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UXT FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=G1P:ALPHA~~-~~D-GLUCOSE-1-PHOSPHATE'>G1P</scene>~~, ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1ky8\|1ky8]], [[1uxn\|1uxn]], [[1uxp\|1uxp]], [[1uxq\|1uxq]], [[1uxr\|1uxr]], [[1uxu\|1uxu]], [[1uxv\|1uxv]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks'>[https~~:~~//en.wikipedia.org/wiki/Glyceraldehyde~~-3-~~phosphate_dehydrogenase_(NADP(+)) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+))], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.9 1.2.1.9]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxt OCA], [https://pdbe.org/1uxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uxt RCSB], [https://www.ebi.ac.uk/pdbsum/1uxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uxt ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxt OCA], [https://pdbe.org/1uxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uxt RCSB], [https://www.ebi.ac.uk/pdbsum/1uxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uxt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GAPN_THETE GAPN_THETE]] Catalyzes the irreversible NAD(P)-dependent non-phosphorylating oxidation of glyceraldehyde-3-phosphate (GAP) to 3-phosphoglycerate (3PG). It is highly specific for D-GAP.<ref>PMID:3121324</ref> <ref>PMID:9497334</ref>	+	[https://www.uniprot.org/uniprot/GAPN_THETE GAPN_THETE] Catalyzes the irreversible NAD(P)-dependent non-phosphorylating oxidation of glyceraldehyde-3-phosphate (GAP) to 3-phosphoglycerate (3PG). It is highly specific for D-GAP.<ref>PMID:3121324</ref> <ref>PMID:9497334</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 35583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Hensel, R~~]]	+	[[Category: Thermoproteus tenax]]
-	[[Category: ~~Lorentzen, E~~]]	+	[[Category: Hensel R]]
-	[[Category: ~~Pohl,~~ E]]	+	[[Category: Lorentzen E]]
-	[[Category: ~~Aldh]]~~	+	[[Category: Pohl E]]
-	~~[[Category: Catatysis]]~~	+
-	~~[[Category: Gapn]]~~	+
-	~~[[Category: Glucose 1-phosphate]]~~	+
-	~~[[Category: Glycolysis]]~~	+
-	~~[[Category: Nad]]~~	+
-	~~[[Category: Oxidoreductase]]~~	+
-	~~[[Category: Regulation~~]]	+

OCA at 06:05, 28 April 2021

OCA — Wed, 28 Apr 2021 06:05:38 GMT

←Older revision		Revision as of 06:05, 28 April 2021
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	<StructureSection load='1uxt' size='340' side='right'caption='[[1uxt]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1uxt' size='340' side='right'caption='[[1uxt]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1uxt]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_35583 Atcc 35583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1UXT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1uxt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_35583 Atcc 35583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UXT FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ky8\|1ky8]], [[1uxn\|1uxn]], [[1uxp\|1uxp]], [[1uxq\|1uxq]], [[1uxr\|1uxr]], [[1uxu\|1uxu]], [[1uxv\|1uxv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1ky8\|1ky8]], [[1uxn\|1uxn]], [[1uxp\|1uxp]], [[1uxq\|1uxq]], [[1uxr\|1uxr]], [[1uxu\|1uxu]], [[1uxv\|1uxv]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+)) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+))], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.9 1.2.1.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+)) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+))], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.9 1.2.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1uxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxt OCA], [~~http~~://pdbe.org/1uxt PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1uxt RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1uxt PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1uxt ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxt OCA], [https://pdbe.org/1uxt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uxt RCSB], [https://www.ebi.ac.uk/pdbsum/1uxt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uxt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GAPN_THETE GAPN_THETE]] Catalyzes the irreversible NAD(P)-dependent non-phosphorylating oxidation of glyceraldehyde-3-phosphate (GAP) to 3-phosphoglycerate (3PG). It is highly specific for D-GAP.<ref>PMID:3121324</ref> <ref>PMID:9497334</ref>	+	[[https://www.uniprot.org/uniprot/GAPN_THETE GAPN_THETE]] Catalyzes the irreversible NAD(P)-dependent non-phosphorylating oxidation of glyceraldehyde-3-phosphate (GAP) to 3-phosphoglycerate (3PG). It is highly specific for D-GAP.<ref>PMID:3121324</ref> <ref>PMID:9497334</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 07:05, 24 July 2019

OCA — Wed, 24 Jul 2019 07:05:34 GMT

←Older revision		Revision as of 07:05, 24 July 2019
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	==Structural basis for allosteric regulation and substrate specificity of the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) from Thermoproteus tenax==		==Structural basis for allosteric regulation and substrate specificity of the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) from Thermoproteus tenax==
-	<StructureSection load='1uxt' size='340' side='right' caption='[[1uxt]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1uxt' size='340' side='right'caption='[[1uxt]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1uxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35583 Atcc 35583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1uxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35583 Atcc 35583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UXT FirstGlance]. <br>
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Glyceraldehyde-3-~~Phosphate Dehydrogenase~~\|Glyceraldehyde-3-~~Phosphate Dehydrogenase~~]]	+	*[[Glyceraldehyde-3-phosphate dehydrogenase 3D structures\|Glyceraldehyde-3-phosphate dehydrogenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 38:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 35583]]		[[Category: Atcc 35583]]
		+	[[Category: Large Structures]]
	[[Category: Hensel, R]]		[[Category: Hensel, R]]
	[[Category: Lorentzen, E]]		[[Category: Lorentzen, E]]

OCA at 08:05, 28 March 2018

OCA — Wed, 28 Mar 2018 08:05:50 GMT

←Older revision		Revision as of 08:05, 28 March 2018
Line 1:		Line 1:
-	==~~STRUCTURAL BASIS FOR ALLOSTERIC REGULATION AND SUBSTRATE SPECIFICITY OF THE NON~~-~~PHOSPHORYLATING GLYCERALDEHYDE~~-3-~~PHOSPHATE DEHYDROGENASE~~ (GAPN) ~~FROM THERMOPROTEUS TENAX~~==	+
		+	==Structural basis for allosteric regulation and substrate specificity of the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) from Thermoproteus tenax==
	<StructureSection load='1uxt' size='340' side='right' caption='[[1uxt]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1uxt' size='340' side='right' caption='[[1uxt]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ky8\|1ky8]], [[1uxn\|1uxn]], [[1uxp\|1uxp]], [[1uxq\|1uxq]], [[1uxr\|1uxr]], [[1uxu\|1uxu]], [[1uxv\|1uxv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ky8\|1ky8]], [[1uxn\|1uxn]], [[1uxp\|1uxp]], [[1uxq\|1uxq]], [[1uxr\|1uxr]], [[1uxu\|1uxu]], [[1uxv\|1uxv]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+)) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.9 1.2.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+)) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.9 1.2.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxt OCA], [http://pdbe.org/1uxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uxt RCSB], [http://www.ebi.ac.uk/pdbsum/1uxt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxt OCA], [http://pdbe.org/1uxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uxt RCSB], [http://www.ebi.ac.uk/pdbsum/1uxt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1uxt ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ux/1uxt_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ux/1uxt_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 10:04, 9 February 2016

OCA — Tue, 09 Feb 2016 10:04:44 GMT

←Older revision		Revision as of 10:04, 9 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1uxt ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 21:09, 10 September 2015

OCA — Thu, 10 Sep 2015 21:09:20 GMT

←Older revision		Revision as of 21:09, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1uxt' size='340' side='right' caption='[[1uxt]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1uxt' size='340' side='right' caption='[[1uxt]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1uxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermoproteus_tenax Thermoproteus tenax~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UXT FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1uxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_35583 Atcc 35583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UXT FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ky8\|1ky8]], [[1uxn\|1uxn]], [[1uxp\|1uxp]], [[1uxq\|1uxq]], [[1uxr\|1uxr]], [[1uxu\|1uxu]], [[1uxv\|1uxv]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ky8\|1ky8]], [[1uxn\|1uxn]], [[1uxp\|1uxp]], [[1uxq\|1uxq]], [[1uxr\|1uxr]], [[1uxu\|1uxu]], [[1uxv\|1uxv]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+)) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.9 1.2.1.9] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+)) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.9 1.2.1.9] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uxt RCSB], [http://www.ebi.ac.uk/pdbsum/1uxt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxt OCA], [http://pdbe.org/1uxt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uxt RCSB], [http://www.ebi.ac.uk/pdbsum/1uxt PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GAPN_THETE GAPN_THETE]] Catalyzes the irreversible NAD(P)-dependent non-phosphorylating oxidation of glyceraldehyde-3-phosphate (GAP) to 3-phosphoglycerate (3PG). It is highly specific for D-GAP.<ref>PMID:3121324</ref> <ref>PMID:9497334</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 26:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1uxt" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Thermoproteus tenax~~]]	+	[[Category: Atcc 35583]]
	[[Category: Hensel, R]]		[[Category: Hensel, R]]
	[[Category: Lorentzen, E]]		[[Category: Lorentzen, E]]

OCA at 12:59, 6 January 2015

OCA — Tue, 06 Jan 2015 12:59:56 GMT

←Older revision		Revision as of 12:59, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1uxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UXT FirstGlance]. <br>		<table><tr><td colspan='2'>[[1uxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UXT FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ky8\|1ky8]], [[1uxn\|1uxn]], [[1uxp\|1uxp]], [[1uxq\|1uxq]], [[1uxr\|1uxr]], [[1uxu\|1uxu]], [[1uxv\|1uxv]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ky8\|1ky8]], [[1uxn\|1uxn]], [[1uxp\|1uxp]], [[1uxq\|1uxq]], [[1uxr\|1uxr]], [[1uxu\|1uxu]], [[1uxv\|1uxv]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+)) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.9 1.2.1.9] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+)) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.9 1.2.1.9] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uxt RCSB], [http://www.ebi.ac.uk/pdbsum/1uxt PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uxt RCSB], [http://www.ebi.ac.uk/pdbsum/1uxt PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Thermoproteus tenax]]		[[Category: Thermoproteus tenax]]
-	[[Category: Hensel, R.]]	+	[[Category: Hensel, R]]
-	[[Category: Lorentzen, E.]]	+	[[Category: Lorentzen, E]]
-	[[Category: Pohl, E.]]	+	[[Category: Pohl, E]]
	[[Category: Aldh]]		[[Category: Aldh]]
	[[Category: Catatysis]]		[[Category: Catatysis]]

OCA at 21:35, 29 September 2014

OCA — Mon, 29 Sep 2014 21:35:09 GMT

←Older revision		Revision as of 21:35, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1uxt.~~png~~\|~~left~~\|200px]]	+	==STRUCTURAL BASIS FOR ALLOSTERIC REGULATION AND SUBSTRATE SPECIFICITY OF THE NON-PHOSPHORYLATING GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GAPN) FROM THERMOPROTEUS TENAX==
		+	<StructureSection load='1uxt' size='340' side='right' caption='[[1uxt]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1uxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UXT FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=G1P:ALPHA-D-GLUCOSE-1-PHOSPHATE'>G1P</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1ky8\|1ky8]], [[1uxn\|1uxn]], [[1uxp\|1uxp]], [[1uxq\|1uxq]], [[1uxr\|1uxr]], [[1uxu\|1uxu]], [[1uxv\|1uxv]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+)) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+))], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.9 1.2.1.9] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uxt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uxt OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1uxt RCSB], [http://www.ebi.ac.uk/pdbsum/1uxt PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ux/1uxt_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GAPN) of the hyperthermophilic Archaeum Thermoproteus tenax is a member of the superfamily of aldehyde dehydrogenases (ALDH). GAPN catalyses the irreversible oxidation of glyceraldehyde 3-phosphate (GAP) to 3-phosphoglycerate in the modified glycolytic pathway of this organism. In contrast to other members of the ALDH superfamily, GAPN from T.tenax (Tt-GAPN) is regulated by a number of intermediates and metabolites. In the NAD-dependent oxidation of GAP, glucose 1-phosphate, fructose 6-phosphate, AMP and ADP increase the affinity for the cosubstrate, whereas ATP, NADP, NADPH and NADH decrease it leaving, however, the catalytic rate virtually unaltered. As we show here, the enzyme also uses NADP as a cosubstrate, displaying, however, unusual discontinuous saturation kinetics indicating different cosubstrate affinities and/or reactivities of the four active sites of the protein tetramer caused by cooperative effects. Furthermore, in the NADP-dependent reaction the presence of activators decreases the overall S0.5 and increases Vmax by a factor of 3. To explore the structural basis for the different effects of both pyridine nucleotides we solved the crystal structure of Tt-GAPN in complex with NAD at 2.2 A resolution and compared it to the binary Tt-GAPN-NADPH structure. Although both pyridine nucleotides show a similar binding mode, NADPH appears to be more tightly bound to the protein via the 2' phosphate moiety. Moreover, we present four co-crystal structures with the activating molecules glucose 1-phosphate, fructose 6-phosphate, AMP and ADP determined at resolutions ranging from 2.3 A to 2.6 A. These crystal structures reveal a common regulatory site able to accommodate the different activators. A phosphate-binding pocket serves as an anchor point ensuring similar binding geometry. The observed conformational changes upon activator binding are discussed in terms of allosteric regulation. Furthermore, we present a crystal structure of Tt-GAPN in complex with the substrate D-GAP at 2.3 A resolution, which allows us to analyse the structural basis for substrate binding, the mechanism of catalysis as well as the stereoselectivity of the enzymatic reaction.

-	~~{{STRUCTURE_1uxt\| PDB=1uxt \| SCENE= }}~~	+	Structural Basis of allosteric regulation and substrate specificity of the non-phosphorylating glyceraldehyde 3-Phosphate dehydrogenase from Thermoproteus tenax.,Lorentzen E, Hensel R, Knura T, Ahmed H, Pohl E J Mol Biol. 2004 Aug 13;341(3):815-28. PMID:15288789<ref>PMID:15288789</ref>

-	~~===STRUCTURAL BASIS FOR ALLOSTERIC REGULATION AND SUBSTRATE SPECIFICITY OF THE NON-PHOSPHORYLATING GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GAPN) FROM THERMOPROTEUS TENAX===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>

-	~~{{ABSTRACT_PUBMED_15288789}}~~	+	==See Also==
-		+	*[[Glyceraldehyde-3-Phosphate Dehydrogenase\|Glyceraldehyde-3-Phosphate Dehydrogenase]]
-	==~~About this Structure~~==	+	== References ==
-	[[~~1uxt~~]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA].	+	<references/>
-		+	__TOC__
-	==~~Reference~~==	+	</StructureSection>
-	<~~ref group="xtra">PMID:015288789<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Thermoproteus tenax]]		[[Category: Thermoproteus tenax]]
	[[Category: Hensel, R.]]		[[Category: Hensel, R.]]

OCA: Protected "1uxt" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 12:55:03 GMT

Protected "1uxt" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:55, 5 January 2013

OCA at 12:54, 5 January 2013

OCA — Sat, 05 Jan 2013 12:54:57 GMT

←Older revision		Revision as of 12:54, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1uxt.png\|left\|200px]]		[[Image:1uxt.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1uxt", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1uxt\| PDB=1uxt \| SCENE= }}		{{STRUCTURE_1uxt\| PDB=1uxt \| SCENE= }}

	===STRUCTURAL BASIS FOR ALLOSTERIC REGULATION AND SUBSTRATE SPECIFICITY OF THE NON-PHOSPHORYLATING GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GAPN) FROM THERMOPROTEUS TENAX===		===STRUCTURAL BASIS FOR ALLOSTERIC REGULATION AND SUBSTRATE SPECIFICITY OF THE NON-PHOSPHORYLATING GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (GAPN) FROM THERMOPROTEUS TENAX===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15288789}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15288789 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15288789}}		{{ABSTRACT_PUBMED_15288789}}

	==About this Structure==		==About this Structure==
-	~~1UXT~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA].	+	[[1uxt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermoproteus_tenax Thermoproteus tenax]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UXT OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15288789~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015288789</ref><references group="xtra"/>
	[[Category: Thermoproteus tenax]]		[[Category: Thermoproteus tenax]]
	[[Category: Hensel, R.]]		[[Category: Hensel, R.]]
Line 36:		Line 24:
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]
	[[Category: Regulation]]		[[Category: Regulation]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 09:00:07 2009''