1v2j - Revision history

OCA at 23:56, 27 December 2023

OCA — Wed, 27 Dec 2023 23:56:41 GMT

←Older revision		Revision as of 23:56, 27 December 2023
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	<StructureSection load='1v2j' size='340' side='right'caption='[[1v2j]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1v2j' size='340' side='right'caption='[[1v2j]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1v2j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V2J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1v2j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V2J FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>~~, ~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1v2k\|1v2k]], [[1v2l\|1v2l]], [[1v2m\|1v2m]], [[1v2n\|1v2n]], [[1v2o\|1v2o]], [[1v2p\|1v2p]], [[1v2q\|1v2q]], [[1v2r\|1v2r]], [[1v2s\|1v2s]], [[1v2t\|1v2t]], [[1v2u\|1v2u]], [[1v2v\|1v2v]], [[1v2w\|1v2w]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2j OCA], [https://pdbe.org/1v2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v2j RCSB], [https://www.ebi.ac.uk/pdbsum/1v2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v2j ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2j OCA], [https://pdbe.org/1v2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v2j RCSB], [https://www.ebi.ac.uk/pdbsum/1v2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v2j ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/TRY1_BOVIN TRY1_BOVIN]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Trypsin]]~~	+	[[Category: Klebe G]]
-	[[Category: Klebe, G]]	+	[[Category: Rauh D]]
-	[[Category: Rauh, D]]	+	[[Category: Stubbs MT]]
-	[[Category: Stubbs~~, M T]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Serine protease]]~~	+
-	~~[[Category: Serine proteinase~~]]	+

OCA at 06:23, 6 October 2021

OCA — Wed, 06 Oct 2021 06:23:09 GMT

←Older revision		Revision as of 06:23, 6 October 2021
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	<StructureSection load='1v2j' size='340' side='right'caption='[[1v2j]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1v2j' size='340' side='right'caption='[[1v2j]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1v2j]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1V2J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1v2j]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1V2J FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1v2k\|1v2k]], [[1v2l\|1v2l]], [[1v2m\|1v2m]], [[1v2n\|1v2n]], [[1v2o\|1v2o]], [[1v2p\|1v2p]], [[1v2q\|1v2q]], [[1v2r\|1v2r]], [[1v2s\|1v2s]], [[1v2t\|1v2t]], [[1v2u\|1v2u]], [[1v2v\|1v2v]], [[1v2w\|1v2w]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1v2k\|1v2k]], [[1v2l\|1v2l]], [[1v2m\|1v2m]], [[1v2n\|1v2n]], [[1v2o\|1v2o]], [[1v2p\|1v2p]], [[1v2q\|1v2q]], [[1v2r\|1v2r]], [[1v2s\|1v2s]], [[1v2t\|1v2t]], [[1v2u\|1v2u]], [[1v2v\|1v2v]], [[1v2w\|1v2w]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1v2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2j OCA], [~~http~~://pdbe.org/1v2j PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1v2j RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1v2j PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1v2j ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1v2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2j OCA], [https://pdbe.org/1v2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1v2j RCSB], [https://www.ebi.ac.uk/pdbsum/1v2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1v2j ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	==See Also==		==See Also==
-	*[[Trypsin\|Trypsin]]	+	*[[Trypsin 3D structures\|Trypsin 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 13:14, 1 January 2020

OCA — Wed, 01 Jan 2020 13:14:24 GMT

←Older revision		Revision as of 13:14, 1 January 2020
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	==BENZAMIDINE IN COMPLEX WITH BOVINE TRYPSIN VARIANT X(SSRI)bT.C1==		==BENZAMIDINE IN COMPLEX WITH BOVINE TRYPSIN VARIANT X(SSRI)bT.C1==
-	<StructureSection load='1v2j' size='340' side='right' caption='[[1v2j]], [[Resolution\|resolution]] 1.90Å' scene=''>	+	<StructureSection load='1v2j' size='340' side='right'caption='[[1v2j]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1v2j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V2J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1v2j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V2J FirstGlance]. <br>
Line 36:		Line 36:
	</StructureSection>		</StructureSection>
	[[Category: Bovin]]		[[Category: Bovin]]
		+	[[Category: Large Structures]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
	[[Category: Klebe, G]]		[[Category: Klebe, G]]

OCA at 08:03, 28 March 2018

OCA — Wed, 28 Mar 2018 08:03:50 GMT

←Older revision		Revision as of 08:03, 28 March 2018
Line 1:		Line 1:
		+
	==BENZAMIDINE IN COMPLEX WITH BOVINE TRYPSIN VARIANT X(SSRI)bT.C1==		==BENZAMIDINE IN COMPLEX WITH BOVINE TRYPSIN VARIANT X(SSRI)bT.C1==
	<StructureSection load='1v2j' size='340' side='right' caption='[[1v2j]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1v2j' size='340' side='right' caption='[[1v2j]], [[Resolution\|resolution]] 1.90Å' scene=''>
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	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1v2k\|1v2k]], [[1v2l\|1v2l]], [[1v2m\|1v2m]], [[1v2n\|1v2n]], [[1v2o\|1v2o]], [[1v2p\|1v2p]], [[1v2q\|1v2q]], [[1v2r\|1v2r]], [[1v2s\|1v2s]], [[1v2t\|1v2t]], [[1v2u\|1v2u]], [[1v2v\|1v2v]], [[1v2w\|1v2w]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1v2k\|1v2k]], [[1v2l\|1v2l]], [[1v2m\|1v2m]], [[1v2n\|1v2n]], [[1v2o\|1v2o]], [[1v2p\|1v2p]], [[1v2q\|1v2q]], [[1v2r\|1v2r]], [[1v2s\|1v2s]], [[1v2t\|1v2t]], [[1v2u\|1v2u]], [[1v2v\|1v2v]], [[1v2w\|1v2w]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2j OCA], [http://pdbe.org/1v2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1v2j RCSB], [http://www.ebi.ac.uk/pdbsum/1v2j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2j OCA], [http://pdbe.org/1v2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1v2j RCSB], [http://www.ebi.ac.uk/pdbsum/1v2j PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1v2j ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v2/1v2j_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v2/1v2j_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 23:21, 7 February 2016

OCA — Sun, 07 Feb 2016 23:21:55 GMT

←Older revision		Revision as of 23:21, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1v2j ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 05:47, 10 September 2015

OCA — Thu, 10 Sep 2015 05:47:40 GMT

←Older revision		Revision as of 05:47, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1v2j' size='340' side='right' caption='[[1v2j]], [[Resolution\|resolution]] 1.90Å' scene=''>		<StructureSection load='1v2j' size='340' side='right' caption='[[1v2j]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1v2j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V2J FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1v2j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V2J FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1v2k\|1v2k]], [[1v2l\|1v2l]], [[1v2m\|1v2m]], [[1v2n\|1v2n]], [[1v2o\|1v2o]], [[1v2p\|1v2p]], [[1v2q\|1v2q]], [[1v2r\|1v2r]], [[1v2s\|1v2s]], [[1v2t\|1v2t]], [[1v2u\|1v2u]], [[1v2v\|1v2v]], [[1v2w\|1v2w]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1v2k\|1v2k]], [[1v2l\|1v2l]], [[1v2m\|1v2m]], [[1v2n\|1v2n]], [[1v2o\|1v2o]], [[1v2p\|1v2p]], [[1v2q\|1v2q]], [[1v2r\|1v2r]], [[1v2s\|1v2s]], [[1v2t\|1v2t]], [[1v2u\|1v2u]], [[1v2v\|1v2v]], [[1v2w\|1v2w]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1v2j RCSB], [http://www.ebi.ac.uk/pdbsum/1v2j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2j OCA], [http://pdbe.org/1v2j PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1v2j RCSB], [http://www.ebi.ac.uk/pdbsum/1v2j PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1v2j" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 34:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bos taurus~~]]	+	[[Category: Bovin]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
	[[Category: Klebe, G]]		[[Category: Klebe, G]]

OCA at 12:56, 6 January 2015

OCA — Tue, 06 Jan 2015 12:56:27 GMT

←Older revision		Revision as of 12:56, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1v2j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V2J FirstGlance]. <br>		<table><tr><td colspan='2'>[[1v2j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V2J FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1v2k\|1v2k]], [[1v2l\|1v2l]], [[1v2m\|1v2m]], [[1v2n\|1v2n]], [[1v2o\|1v2o]], [[1v2p\|1v2p]], [[1v2q\|1v2q]], [[1v2r\|1v2r]], [[1v2s\|1v2s]], [[1v2t\|1v2t]], [[1v2u\|1v2u]], [[1v2v\|1v2v]], [[1v2w\|1v2w]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1v2k\|1v2k]], [[1v2l\|1v2l]], [[1v2m\|1v2m]], [[1v2n\|1v2n]], [[1v2o\|1v2o]], [[1v2p\|1v2p]], [[1v2q\|1v2q]], [[1v2r\|1v2r]], [[1v2s\|1v2s]], [[1v2t\|1v2t]], [[1v2u\|1v2u]], [[1v2v\|1v2v]], [[1v2w\|1v2w]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1v2j RCSB], [http://www.ebi.ac.uk/pdbsum/1v2j PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1v2j RCSB], [http://www.ebi.ac.uk/pdbsum/1v2j PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Trypsin]]		[[Category: Trypsin]]
-	[[Category: Klebe, G.]]	+	[[Category: Klebe, G]]
-	[[Category: Rauh, D.]]	+	[[Category: Rauh, D]]
-	[[Category: Stubbs, M T.]]	+	[[Category: Stubbs, M T]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Serine protease]]		[[Category: Serine protease]]
	[[Category: Serine proteinase]]		[[Category: Serine proteinase]]

OCA at 21:13, 28 September 2014

OCA — Sun, 28 Sep 2014 21:13:49 GMT

←Older revision		Revision as of 21:13, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1v2j.~~png~~\|~~left~~\|200px]]	+	==BENZAMIDINE IN COMPLEX WITH BOVINE TRYPSIN VARIANT X(SSRI)bT.C1==
		+	<StructureSection load='1v2j' size='340' side='right' caption='[[1v2j]], [[Resolution\|resolution]] 1.90Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1v2j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1V2J FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BEN:BENZAMIDINE'>BEN</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1v2k\|1v2k]], [[1v2l\|1v2l]], [[1v2m\|1v2m]], [[1v2n\|1v2n]], [[1v2o\|1v2o]], [[1v2p\|1v2p]], [[1v2q\|1v2q]], [[1v2r\|1v2r]], [[1v2s\|1v2s]], [[1v2t\|1v2t]], [[1v2u\|1v2u]], [[1v2v\|1v2v]], [[1v2w\|1v2w]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Trypsin Trypsin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.4 3.4.21.4] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1v2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1v2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1v2j RCSB], [http://www.ebi.ac.uk/pdbsum/1v2j PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/v2/1v2j_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In order to design selective, high-affinity ligands to a target protein, it is advantageous to understand the structural determinants for protein-ligand complex formation at the atomic level. In a model system, we have successively mapped the factor Xa binding site onto trypsin, showing that certain mutations influence both protein structure and inhibitor specificity. Our previous studies have shown that introduction of the 172SSFI175 sequence of factor Xa into rat or bovine trypsin results in the destabilisation of the intermediate helix with burial of Phe174 (the down conformation). Surface exposure of the latter residue (the up conformation) is critical for the correct formation of the aromatic box found in factor Xa-ligand complexes. In the present study, we investigate the influence of aromatic residues in position 174. Replacement with the bulky tryptophan (SSWI) shows reduced affinity for benzamidine-based inhibitors (1) and (4), whereas removal of the side-chain (alanine, SSAI) or exchange with a hydrophilic residue (arginine, SSRI) leads to a significant loss in affinity for all inhibitors studied. The variants could be crystallised in the presence of different inhibitors in multiple crystal forms. Structural characterisation of the variants revealed three different conformations of the intermediate helix and 175 loop in SSAI (down, up and super-up), as well as a complete disorder of this region in one crystal form of SSRI, suggesting that the compromised affinity of these variants is related to conformational flexibility. The influence of Glu217, peripheral to the ligand-binding site in factor Xa, was investigated. Introduction of Glu217 into trypsin variants containing the SSFI sequence exhibited enhanced affinity for the factor Xa ligands (2) and (3). The crystal structures of these variants also exhibited the down and super-up conformations, the latter of which could be converted to up upon soaking and binding of inhibitor (2). The improved affinity of the Glu217-containing variants appears to be due to a shift towards the up conformation. Thus, the reduction in affinity caused by conformational variability of the protein target can be partially or wholly offset by compensatory binding to the up conformation. The insights provided by these studies will be helpful in improving our understanding of ligand binding for the drug design process.

-	~~{{STRUCTURE_1v2j\| PDB=1v2j \| SCENE= }}~~	+	Understanding protein-ligand interactions: the price of protein flexibility.,Rauh D, Klebe G, Stubbs MT J Mol Biol. 2004 Jan 30;335(5):1325-41. PMID:14729347<ref>PMID:14729347</ref>

-	~~===BENZAMIDINE IN COMPLEX WITH BOVINE TRYPSIN VARIANT X(SSRI)bT.C1===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_14729347}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1v2j]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA].~~	+

	==See Also==		==See Also==
	*[[Trypsin\|Trypsin]]		*[[Trypsin\|Trypsin]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:014729347<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Trypsin]]		[[Category: Trypsin]]

OCA at 08:08, 20 October 2012

OCA — Sat, 20 Oct 2012 08:08:47 GMT

←Older revision		Revision as of 08:08, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1v2j]] is a 1 chain structure ~~of [[Trypsin]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA].	+	[[1v2j]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1V2J OCA].

	==See Also==		==See Also==

OCA: Protected "1v2j" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 18:36:14 GMT

Protected "1v2j" [edit=sysop:move=sysop]

←Older revision

Revision as of 18:36, 25 July 2012