1vlq - Revision history

OCA at 06:41, 25 January 2023

OCA — Wed, 25 Jan 2023 06:41:40 GMT

←Older revision		Revision as of 06:41, 25 January 2023
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	<StructureSection load='1vlq' size='340' side='right'caption='[[1vlq]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1vlq' size='340' side='right'caption='[[1vlq]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1vlq]] is a 12 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Thema Thema~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1VLQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1vlq]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VLQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>~~</td></tr>~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	~~<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat">~~<scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene~~></td></tr>~~	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlq OCA], [https://pdbe.org/1vlq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vlq RCSB], [https://www.ebi.ac.uk/pdbsum/1vlq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vlq ProSAT], [https://www.topsan.org/Proteins/JCSG/1vlq TOPSAN]</span></td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM0077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>~~	+
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cephalosporin-C_deacetylase Cephalosporin-C deacetylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.41 3.1.1.41] </span></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1vlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlq OCA], [~~http~~://pdbe.org/1vlq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1vlq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1vlq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1vlq ProSAT], [~~http~~://www.topsan.org/Proteins/JCSG/1vlq TOPSAN]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CAH_THEMA CAH_THEMA]] Esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylo-oligosaccharides and cephalosporin C. Has no activity towards polymeric acetylated xylan, 4-methylumbelliferyl acetate or alpha-naphthyl acetate. Able to catalyze rapid hydrolysis of a range of substrates preferably with acetate groups, independent of the alcohol moiety. Exhibits a narrow selectivity for short chain acyl esters (C2-C3). Displays broad substrate specificity by hydrolyzing acetate at 2, 3, and 4 positions of 4-nitrophenyl-beta-D-xylopyranoside (pNP-Xyl) with similar efficiency. Cannot cleave amide linkages.<ref>PMID:21255309</ref> <ref>PMID:22411095</ref> <ref>PMID:22659119</ref>	+	[https://www.uniprot.org/uniprot/CAH_THEMA CAH_THEMA] Esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylo-oligosaccharides and cephalosporin C. Has no activity towards polymeric acetylated xylan, 4-methylumbelliferyl acetate or alpha-naphthyl acetate. Able to catalyze rapid hydrolysis of a range of substrates preferably with acetate groups, independent of the alcohol moiety. Exhibits a narrow selectivity for short chain acyl esters (C2-C3). Displays broad substrate specificity by hydrolyzing acetate at 2, 3, and 4 positions of 4-nitrophenyl-beta-D-xylopyranoside (pNP-Xyl) with similar efficiency. Cannot cleave amide linkages.<ref>PMID:21255309</ref> <ref>PMID:22411095</ref> <ref>PMID:22659119</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cephalosporin-C deacetylase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Thema]]~~	+	[[Category: Thermotoga maritima MSB8]]
-	~~[[Category: Structural genomic]]~~	+
-	~~[[Category: Acetyl xylan esterase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Jcsg]]~~	+
-	~~[[Category: PSI, Protein structure initiative]]~~	+
-	~~[[Category: Tm0077~~]]	+

OCA at 07:39, 30 December 2020

OCA — Wed, 30 Dec 2020 07:39:34 GMT

←Older revision		Revision as of 07:39, 30 December 2020
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	==Crystal structure of Acetyl xylan esterase (TM0077) from Thermotoga maritima at 2.10 A resolution==		==Crystal structure of Acetyl xylan esterase (TM0077) from Thermotoga maritima at 2.10 A resolution==
-	<StructureSection load='1vlq' size='340' side='right' caption='[[1vlq]], [[Resolution\|resolution]] 2.10Å' scene=''>	+	<StructureSection load='1vlq' size='340' side='right'caption='[[1vlq]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1vlq]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLQ OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1VLQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1vlq]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1VLQ FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM0077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM0077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cephalosporin-C_deacetylase Cephalosporin-C deacetylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.41 3.1.1.41] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cephalosporin-C_deacetylase Cephalosporin-C deacetylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.41 3.1.1.41] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1vlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlq OCA], [http://pdbe.org/1vlq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vlq RCSB], [http://www.ebi.ac.uk/pdbsum/1vlq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1vlq ProSAT], [http://www.topsan.org/Proteins/JCSG/1vlq TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1vlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlq OCA], [http://pdbe.org/1vlq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vlq RCSB], [http://www.ebi.ac.uk/pdbsum/1vlq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1vlq ProSAT], [http://www.topsan.org/Proteins/JCSG/1vlq TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	==See Also==		==See Also==
-	*[[Acetylxylan esterase\|Acetylxylan esterase]]	+	*[[Acetylxylan esterase 3D structures\|Acetylxylan esterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 39:
	</StructureSection>		</StructureSection>
	[[Category: Cephalosporin-C deacetylase]]		[[Category: Cephalosporin-C deacetylase]]
		+	[[Category: Large Structures]]
	[[Category: Thema]]		[[Category: Thema]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]

OCA at 07:13, 4 April 2018

OCA — Wed, 04 Apr 2018 07:13:42 GMT

←Older revision		Revision as of 07:13, 4 April 2018
Line 1:		Line 1:
		+
	==Crystal structure of Acetyl xylan esterase (TM0077) from Thermotoga maritima at 2.10 A resolution==		==Crystal structure of Acetyl xylan esterase (TM0077) from Thermotoga maritima at 2.10 A resolution==
	<StructureSection load='1vlq' size='340' side='right' caption='[[1vlq]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1vlq' size='340' side='right' caption='[[1vlq]], [[Resolution\|resolution]] 2.10Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM0077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM0077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cephalosporin-C_deacetylase Cephalosporin-C deacetylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.41 3.1.1.41] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cephalosporin-C_deacetylase Cephalosporin-C deacetylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.41 3.1.1.41] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlq OCA], [http://pdbe.org/1vlq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vlq RCSB], [http://www.ebi.ac.uk/pdbsum/1vlq PDBsum], [http://www.topsan.org/Proteins/JCSG/1vlq TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlq OCA], [http://pdbe.org/1vlq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vlq RCSB], [http://www.ebi.ac.uk/pdbsum/1vlq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1vlq ProSAT], [http://www.topsan.org/Proteins/JCSG/1vlq TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vl/1vlq_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vl/1vlq_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 22:53, 8 February 2016

OCA — Mon, 08 Feb 2016 22:53:59 GMT

←Older revision		Revision as of 22:53, 8 February 2016
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vlq ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 16:08, 10 September 2015

OCA — Thu, 10 Sep 2015 16:08:41 GMT

←Older revision		Revision as of 16:08, 10 September 2015
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	<StructureSection load='1vlq' size='340' side='right' caption='[[1vlq]], [[Resolution\|resolution]] 2.10Å' scene=''>		<StructureSection load='1vlq' size='340' side='right' caption='[[1vlq]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1vlq]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Thermotoga_maritima Thermotoga maritima~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1vlq]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM0077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=~~2336 Thermotoga maritima~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM0077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cephalosporin-C_deacetylase Cephalosporin-C deacetylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.41 3.1.1.41] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cephalosporin-C_deacetylase Cephalosporin-C deacetylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.41 3.1.1.41] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vlq RCSB], [http://www.ebi.ac.uk/pdbsum/1vlq PDBsum], [http://www.topsan.org/Proteins/JCSG/1vlq TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlq OCA], [http://pdbe.org/1vlq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vlq RCSB], [http://www.ebi.ac.uk/pdbsum/1vlq PDBsum], [http://www.topsan.org/Proteins/JCSG/1vlq TOPSAN]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[http://www.uniprot.org/uniprot/~~Q9WXT2_THEMA Q9WXT2_THEMA~~]] Esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylo-oligosaccharides and cephalosporin C. Has no activity towards polymeric acetylated xylan, 4-methylumbelliferyl acetate or alpha-naphthyl acetate. Able to catalyze rapid hydrolysis of a range of substrates preferably with acetate groups, independent of the alcohol moiety. Exhibits a narrow selectivity for short chain acyl esters (C2-C3). Displays broad substrate specificity by hydrolyzing acetate at 2, 3, and 4 positions of 4-nitrophenyl-beta-D-xylopyranoside (pNP-Xyl) with similar efficiency. Cannot cleave amide linkages.<ref>PMID:21255309</ref> <ref>PMID:~~22659119~~</ref> <ref>PMID:~~22411095~~</ref>	+	[[http://www.uniprot.org/uniprot/CAH_THEMA CAH_THEMA]] Esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylo-oligosaccharides and cephalosporin C. Has no activity towards polymeric acetylated xylan, 4-methylumbelliferyl acetate or alpha-naphthyl acetate. Able to catalyze rapid hydrolysis of a range of substrates preferably with acetate groups, independent of the alcohol moiety. Exhibits a narrow selectivity for short chain acyl esters (C2-C3). Displays broad substrate specificity by hydrolyzing acetate at 2, 3, and 4 positions of 4-nitrophenyl-beta-D-xylopyranoside (pNP-Xyl) with similar efficiency. Cannot cleave amide linkages.<ref>PMID:21255309</ref> <ref>PMID:22411095</ref> <ref>PMID:22659119</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1vlq" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 37:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Cephalosporin-C deacetylase]]		[[Category: Cephalosporin-C deacetylase]]
-	[[Category: ~~Thermotoga maritima~~]]	+	[[Category: Thema]]
	[[Category: Structural genomic]]		[[Category: Structural genomic]]
	[[Category: Acetyl xylan esterase]]		[[Category: Acetyl xylan esterase]]

OCA at 02:41, 25 December 2014

OCA — Thu, 25 Dec 2014 02:41:36 GMT

←Older revision		Revision as of 02:41, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1vlq]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLQ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1vlq]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLQ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM0077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM0077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cephalosporin-C_deacetylase Cephalosporin-C deacetylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.41 3.1.1.41] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cephalosporin-C_deacetylase Cephalosporin-C deacetylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.41 3.1.1.41] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vlq RCSB], [http://www.ebi.ac.uk/pdbsum/1vlq PDBsum], [http://www.topsan.org/Proteins/JCSG/1vlq TOPSAN]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vlq RCSB], [http://www.ebi.ac.uk/pdbsum/1vlq PDBsum], [http://www.topsan.org/Proteins/JCSG/1vlq TOPSAN]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/Q9WXT2_THEMA Q9WXT2_THEMA]] Esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylo-oligosaccharides and cephalosporin C. Has no activity towards polymeric acetylated xylan, 4-methylumbelliferyl acetate or alpha-naphthyl acetate. Able to catalyze rapid hydrolysis of a range of substrates preferably with acetate groups, independent of the alcohol moiety. Exhibits a narrow selectivity for short chain acyl esters (C2-C3). Displays broad substrate specificity by hydrolyzing acetate at 2, 3, and 4 positions of 4-nitrophenyl-beta-D-xylopyranoside (pNP-Xyl) with similar efficiency. Cannot cleave amide linkages.<ref>PMID:21255309</ref> <ref>PMID:22659119</ref> <ref>PMID:22411095</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Cephalosporin-C deacetylase]]		[[Category: Cephalosporin-C deacetylase]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]
-	[[Category: ~~JCSG, Joint Center for~~ Structural ~~Genomics.~~]]	+	[[Category: Structural genomic]]
	[[Category: Acetyl xylan esterase]]		[[Category: Acetyl xylan esterase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Jcsg]]		[[Category: Jcsg]]
-	[[Category: ~~Joint center for structural genomic]]~~	+	[[Category: PSI, Protein structure initiative]]
-	~~[[Category:~~ Protein structure initiative]]	+
-	~~[[Category: Psi]]~~	+
-	~~[[Category: Structural genomic~~]]	+
	[[Category: Tm0077]]		[[Category: Tm0077]]

OCA at 19:18, 28 September 2014

OCA — Sun, 28 Sep 2014 19:18:03 GMT

←Older revision		Revision as of 19:18, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1vlq.~~png~~\|~~left~~\|200px]]	+	==Crystal structure of Acetyl xylan esterase (TM0077) from Thermotoga maritima at 2.10 A resolution==
		+	<StructureSection load='1vlq' size='340' side='right' caption='[[1vlq]], [[Resolution\|resolution]] 2.10Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1vlq]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLQ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TM0077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 Thermotoga maritima])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cephalosporin-C_deacetylase Cephalosporin-C deacetylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.1.41 3.1.1.41] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlq OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vlq RCSB], [http://www.ebi.ac.uk/pdbsum/1vlq PDBsum], [http://www.topsan.org/Proteins/JCSG/1vlq TOPSAN]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vl/1vlq_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	TM0077 from Thermotoga maritima is a member of the carbohydrate esterase family 7 and is active on a variety of acetylated compounds, including cephalosporin C. TM0077 esterase activity is confined to short-chain acyl esters (C2-C3), and is optimal around 100 degrees C and pH 7.5. The positional specificity of TM0077 was investigated using 4-nitrophenyl-beta-D-xylopyranoside monoacetates as substrates in a beta-xylosidase-coupled assay. TM0077 hydrolyzes acetate at positions 2, 3, and 4 with equal efficiency. No activity was detected on xylan or acetylated xylan, which implies that TM0077 is an acetyl esterase and not an acetyl xylan esterase as currently annotated. Selenomethionine-substituted and native structures of TM0077 were determined at 2.1 and 2.5 A resolution, respectively, revealing a classic alpha/beta-hydrolase fold. TM0077 assembles into a doughnut-shaped hexamer with small tunnels on either side leading to an inner cavity, which contains the six catalytic centers. Structures of TM0077 with covalently bound phenylmethylsulfonyl fluoride and paraoxon were determined to 2.4 and 2.1 A, respectively, and confirmed that both inhibitors bind covalently to the catalytic serine (Ser188). Upon binding of inhibitor, the catalytic serine adopts an altered conformation, as observed in other esterase and lipases, and supports a previously proposed catalytic mechanism in which Ser hydroxyl rotation prevents reversal of the reaction and allows access of a water molecule for completion of the reaction. Proteins 2012. (c) 2012 Wiley Periodicals, Inc.

-	~~{{STRUCTURE_1vlq\| PDB=1vlq \| SCENE= }}~~	+	Functional and structural characterization of a thermostable acetyl esterase from Thermotoga maritima.,Levisson M, Han GW, Deller MC, Xu Q, Biely P, Hendriks S, Ten Eyck LF, Flensburg C, Roversi P, Miller MD, McMullan D, von Delft F, Kreusch A, Deacon AM, van der Oost J, Lesley SA, Elsliger MA, Kengen SW, Wilson IA Proteins. 2012 Jan 27. doi: 10.1002/prot.24041. PMID:22411095<ref>PMID:22411095</ref>

-	~~===Crystal structure~~ of ~~Acetyl xylan esterase (TM0077) from Thermotoga maritima at 2~~.~~10 A resolution===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_22411095}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1vlq]] is a 12 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org~~/~~wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLQ OCA].~~	+

	==See Also==		==See Also==
	*[[Acetylxylan esterase\|Acetylxylan esterase]]		*[[Acetylxylan esterase\|Acetylxylan esterase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:022411095<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Cephalosporin-C deacetylase]]		[[Category: Cephalosporin-C deacetylase]]
	[[Category: Thermotoga maritima]]		[[Category: Thermotoga maritima]]

OCA at 11:45, 20 October 2012

OCA — Sat, 20 Oct 2012 11:45:22 GMT

←Older revision		Revision as of 11:45, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1vlq]] is a 12 chain structure ~~of [[Acetylxylan esterase]]~~ with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLQ OCA].	+	[[1vlq]] is a 12 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLQ OCA].

	==See Also==		==See Also==

OCA at 06:29, 26 July 2012

OCA — Thu, 26 Jul 2012 06:29:10 GMT

←Older revision		Revision as of 06:29, 26 July 2012
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	[[Image:1vlq.png\|left\|200px]]		[[Image:1vlq.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1vlq\| PDB=1vlq \| SCENE= }}		{{STRUCTURE_1vlq\| PDB=1vlq \| SCENE= }}

	===Crystal structure of Acetyl xylan esterase (TM0077) from Thermotoga maritima at 2.10 A resolution===		===Crystal structure of Acetyl xylan esterase (TM0077) from Thermotoga maritima at 2.10 A resolution===

-
-	<!--
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OCA: Protected "1vlq" [edit=sysop:move=sysop]

OCA — Wed, 23 May 2012 05:07:21 GMT

Protected "1vlq" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:07, 23 May 2012