1vlz - Revision history

OCA at 08:46, 14 February 2024

OCA — Wed, 14 Feb 2024 08:46:31 GMT

←Older revision		Revision as of 08:46, 14 February 2024
Line 3:		Line 3:
	<StructureSection load='1vlz' size='340' side='right'caption='[[1vlz]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='1vlz' size='340' side='right'caption='[[1vlz]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlz OCA], [https://pdbe.org/1vlz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vlz RCSB], [https://www.ebi.ac.uk/pdbsum/1vlz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vlz ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.05Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlz OCA], [https://pdbe.org/1vlz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vlz RCSB], [https://www.ebi.ac.uk/pdbsum/1vlz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vlz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/CHEY_ECOLI CHEY_ECOLI]] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. Overexpression of CheY in association with MotA and MotB improves motility of a ycgR disruption, suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein and the flagellar stator.<ref>PMID:20346719</ref>	+	[https://www.uniprot.org/uniprot/CHEY_ECOLI CHEY_ECOLI] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. Overexpression of CheY in association with MotA and MotB improves motility of a ycgR disruption, suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein and the flagellar stator.<ref>PMID:20346719</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 18:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vlz ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vlz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	Position 87 of the chemotaxis regulatory protein CheY is a highly conserved threonine/serine residue in the response regulator superfamily. A threonine 87 to isoleucine mutant in CheY, identified by its in vivo non-chemotactic phenotype, was also found to be phosphorylatable in vitro. These properties indicate that this mutant does not undergo activation upon phosphorylation. The x-ray crystallographic structure of the threonine to isoleucine CheY mutant has been solved and refined at 2.1-A resolution, to an R factor of 15.6%. Comparison with the wild-type, Mg(2+)-free CheY structure shows that the active site structure is retained, but there are significant localized differences in the backbone conformation distal from the substitution. The presence of the isoleucine side chain also restricts the rotational conformation of another conserved residue in the molecule, tyrosine at position 106. These results provide further evidence for a signaling surface remote from the phosphorylation site of the CheY molecule and implicate threonine 87 and other residues in the post-phosphorylation signaling events.
-
-	Uncoupled phosphorylation and activation in bacterial chemotaxis. The 2.1-A structure of a threonine to isoleucine mutant at position 87 of CheY.,Ganguli S, Wang H, Matsumura P, Volz K J Biol Chem. 1995 Jul 21;270(29):17386-93. PMID:7615544<ref>PMID:7615544</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1vlz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ganguli, S]]	+	[[Category: Ganguli S]]
-	[[Category: Matsumura, P]]	+	[[Category: Matsumura P]]
-	[[Category: Volz, K]]	+	[[Category: Volz K]]
-	[[Category: Wang, H]]	+	[[Category: Wang H]]
-	~~[[Category: Chey]]~~	+
-	~~[[Category: Response regulator]]~~	+
-	~~[[Category: Signal transduction protein]]~~	+
-	~~[[Category: Two-component system~~]]	+

OCA at 09:44, 29 September 2021

OCA — Wed, 29 Sep 2021 09:44:05 GMT

←Older revision		Revision as of 09:44, 29 September 2021
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	<StructureSection load='1vlz' size='340' side='right'caption='[[1vlz]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='1vlz' size='340' side='right'caption='[[1vlz]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1vlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlz OCA], [~~http~~://pdbe.org/1vlz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1vlz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1vlz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1vlz ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlz OCA], [https://pdbe.org/1vlz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vlz RCSB], [https://www.ebi.ac.uk/pdbsum/1vlz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vlz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/CHEY_ECOLI CHEY_ECOLI]] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. Overexpression of CheY in association with MotA and MotB improves motility of a ycgR disruption, suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein and the flagellar stator.<ref>PMID:20346719</ref>	+	[[https://www.uniprot.org/uniprot/CHEY_ECOLI CHEY_ECOLI]] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. Overexpression of CheY in association with MotA and MotB improves motility of a ycgR disruption, suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein and the flagellar stator.<ref>PMID:20346719</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:16, 4 December 2019

OCA — Wed, 04 Dec 2019 11:16:17 GMT

←Older revision		Revision as of 11:16, 4 December 2019
Line 1:		Line 1:

	==UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY==		==UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY==
-	<StructureSection load='1vlz' size='340' side='right' caption='[[1vlz]], [[Resolution\|resolution]] 2.05Å' scene=''>	+	<StructureSection load='1vlz' size='340' side='right'caption='[[1vlz]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vl/1vlz_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vl/1vlz_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 32:		Line 32:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Ganguli, S]]		[[Category: Ganguli, S]]
	[[Category: Matsumura, P]]		[[Category: Matsumura, P]]

OCA at 07:00, 29 November 2017

OCA — Wed, 29 Nov 2017 07:00:47 GMT

←Older revision		Revision as of 07:00, 29 November 2017
Line 1:		Line 1:
		+
	==UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY==		==UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY==
	<StructureSection load='1vlz' size='340' side='right' caption='[[1vlz]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='1vlz' size='340' side='right' caption='[[1vlz]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlz OCA], [http://pdbe.org/1vlz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vlz RCSB], [http://www.ebi.ac.uk/pdbsum/1vlz PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlz OCA], [http://pdbe.org/1vlz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vlz RCSB], [http://www.ebi.ac.uk/pdbsum/1vlz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1vlz ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vlz ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:07, 12 September 2015

OCA — Sat, 12 Sep 2015 02:07:34 GMT

←Older revision		Revision as of 02:07, 12 September 2015
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	<StructureSection load='1vlz' size='340' side='right' caption='[[1vlz]], [[Resolution\|resolution]] 2.05Å' scene=''>		<StructureSection load='1vlz' size='340' side='right' caption='[[1vlz]], [[Resolution\|resolution]] 2.05Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vlz RCSB], [http://www.ebi.ac.uk/pdbsum/1vlz PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlz OCA], [http://pdbe.org/1vlz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vlz RCSB], [http://www.ebi.ac.uk/pdbsum/1vlz PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1vlz" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Ganguli, S]]		[[Category: Ganguli, S]]
	[[Category: Matsumura, P]]		[[Category: Matsumura, P]]

OCA at 22:28, 25 December 2014

OCA — Thu, 25 Dec 2014 22:28:17 GMT

←Older revision		Revision as of 22:28, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>		<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vlz RCSB], [http://www.ebi.ac.uk/pdbsum/1vlz PDBsum]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vlz RCSB], [http://www.ebi.ac.uk/pdbsum/1vlz PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CHEY_ECOLI CHEY_ECOLI]] Involved in the transmission of sensory signals from the chemoreceptors to the flagellar motors. In its active (phosphorylated or acetylated) form, CheY exhibits enhanced binding to a switch component, FliM, at the flagellar motor which induces a change from counterclockwise to clockwise flagellar rotation. Overexpression of CheY in association with MotA and MotB improves motility of a ycgR disruption, suggesting there is an interaction (direct or indirect) between the c-di-GMP-binding flagellar brake protein and the flagellar stator.<ref>PMID:20346719</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 30:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Ganguli, S.]]	+	[[Category: Ganguli, S]]
-	[[Category: Matsumura, P.]]	+	[[Category: Matsumura, P]]
-	[[Category: Volz, K.]]	+	[[Category: Volz, K]]
-	[[Category: Wang, H.]]	+	[[Category: Wang, H]]
	[[Category: Chey]]		[[Category: Chey]]
	[[Category: Response regulator]]		[[Category: Response regulator]]
	[[Category: Signal transduction protein]]		[[Category: Signal transduction protein]]
	[[Category: Two-component system]]		[[Category: Two-component system]]

OCA at 21:47, 29 September 2014

OCA — Mon, 29 Sep 2014 21:47:15 GMT

←Older revision		Revision as of 21:47, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1vlz.~~png\|left~~\|200px]]	+	==UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY==
		+	<StructureSection load='1vlz' size='340' side='right' caption='[[1vlz]], [[Resolution\|resolution]] 2.05Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1vlz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VLZ FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vlz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vlz OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vlz RCSB], [http://www.ebi.ac.uk/pdbsum/1vlz PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vl/1vlz_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Position 87 of the chemotaxis regulatory protein CheY is a highly conserved threonine/serine residue in the response regulator superfamily. A threonine 87 to isoleucine mutant in CheY, identified by its in vivo non-chemotactic phenotype, was also found to be phosphorylatable in vitro. These properties indicate that this mutant does not undergo activation upon phosphorylation. The x-ray crystallographic structure of the threonine to isoleucine CheY mutant has been solved and refined at 2.1-A resolution, to an R factor of 15.6%. Comparison with the wild-type, Mg(2+)-free CheY structure shows that the active site structure is retained, but there are significant localized differences in the backbone conformation distal from the substitution. The presence of the isoleucine side chain also restricts the rotational conformation of another conserved residue in the molecule, tyrosine at position 106. These results provide further evidence for a signaling surface remote from the phosphorylation site of the CheY molecule and implicate threonine 87 and other residues in the post-phosphorylation signaling events.

-	~~{{STRUCTURE_1vlz\| PDB=1vlz \| SCENE= }}~~	+	Uncoupled phosphorylation and activation in bacterial chemotaxis. The 2.1-A structure of a threonine to isoleucine mutant at position 87 of CheY.,Ganguli S, Wang H, Matsumura P, Volz K J Biol Chem. 1995 Jul 21;270(29):17386-93. PMID:7615544<ref>PMID:7615544</ref>

-	~~===UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_7615544}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1vlz]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:007615544<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ganguli, S.]]		[[Category: Ganguli, S.]]

OCA: Protected "1vlz" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 13:16:48 GMT

Protected "1vlz" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:16, 5 January 2013

OCA at 13:16, 5 January 2013

OCA — Sat, 05 Jan 2013 13:16:42 GMT

←Older revision		Revision as of 13:16, 5 January 2013
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-	{{Seed}}
	[[Image:1vlz.png\|left\|200px]]		[[Image:1vlz.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1vlz\| PDB=1vlz \| SCENE= }}		{{STRUCTURE_1vlz\| PDB=1vlz \| SCENE= }}

	===UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY===		===UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_7615544}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 7615544 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_7615544}}		{{ABSTRACT_PUBMED_7615544}}

	==About this Structure==		==About this Structure==
-	~~1VLZ~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA].	+	[[1vlz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~7615544~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:007615544</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Ganguli, S.]]		[[Category: Ganguli, S.]]
Line 31:		Line 19:
	[[Category: Chey]]		[[Category: Chey]]
	[[Category: Response regulator]]		[[Category: Response regulator]]
		+	[[Category: Signal transduction protein]]
	[[Category: Two-component system]]		[[Category: Two-component system]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:44:34 2009''

OCA at 07:44, 18 February 2009

OCA — Wed, 18 Feb 2009 07:44:35 GMT

←Older revision		Revision as of 07:44, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1VLZ is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA].	+	1VLZ is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VLZ OCA].

	==Reference==		==Reference==
-	Uncoupled phosphorylation and activation in bacterial chemotaxis. The 2.1-A structure of a threonine to isoleucine mutant at position 87 of CheY., Ganguli S, Wang H, Matsumura P, Volz K, J Biol Chem. 1995 Jul 21;270(29):17386-93. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/7615544 7615544]~~	+	<ref group="xtra">PMID:7615544</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Ganguli, S.]]		[[Category: Ganguli, S.]]
	[[Category: Matsumura, P.]]		[[Category: Matsumura, P.]]
Line 34:		Line 33:
	[[Category: Two-component system]]		[[Category: Two-component system]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 16~~:03:~~13 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 09:44:34 2009''