1vvo - Revision history

OCA: Redirecting to 4l47

OCA — Wed, 10 Dec 2014 15:04:48 GMT

Redirecting to 4l47

←Older revision		Revision as of 15:04, 10 December 2014
Line 1:		Line 1:
-	~~==Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome==~~	+	#REDIRECT [[4l47]] This PDB entry is obsolete and replaced by 4l47
-	~~<StructureSection load='1vvo' size='340' side='right' caption='~~[[~~1vvo]], [[Resolution\|resolution]] 3.22Å' scene=''>~~	+
-	~~== Structural highlights ==~~	+
-	~~<table><tr><td colspan='2'>[[1vvo~~]] is a 31 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VVO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VVO FirstGlance]. <br>	+
-	~~</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>~~	+
-	~~<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPU:PUROMYCIN-5-MONOPHOSPHATE'>PPU</scene></td></tr>~~	+
-	~~<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vvl\|1vvl]], [[1vvm\|1vvm]], [[1vvn\|1vvn]]</td></tr>~~	+
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vvo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vvo RCSB], [http://www.ebi.ac.uk/pdbsum/1vvo PDBsum]</span></td></tr>	+
-	~~<table>~~	+
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	~~Maintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNASufA6~~ and N1-methylguanosine at position 37 (m1G37) modification-deficient anticodon stem loopPro, both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNASufA6 to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m1G37 modification of tRNAPro also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by ~~the ribosome in maintaining the correct translational reading frame.~~	+
-		+
-	Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.,Maehigashi T, Dunkle JA, Miles SJ, Dunham CM Proc Natl Acad Sci U S A. 2014 Aug 15. pii: 201409436. PMID:25128388<ref>PMID:25128388</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
-	~~__TOC__~~	+
-	~~</StructureSection>~~	+
-	~~[[Category: Thermus thermophilus]]~~	+
-	~~[[Category: Dunham, C M.]]~~	+
-	~~[[Category: Dunkle, J A.]]~~	+
-	~~[[Category: Maehigashi, T.]]~~	+
-	~~[[Category: 16]]~~	+
-	~~[[Category: 23]]~~	+
-	~~[[Category: 30]]~~	+
-	~~[[Category: 70]]~~	+
-	~~[[Category: Protein biosynthesis]]~~	+
-	~~[[Category: Ribosomal subunit]]~~	+
-	~~[[Category: Ribosome]]~~	+
-	~~[[Category: Ribosome]]~~	+
-	~~[[Category: Rna]]~~	+
-	~~[[Category: Transfer rna]]~~	+
-	~~[[Category: Trna]]~~	+

OCA at 03:07, 4 September 2014

OCA — Thu, 04 Sep 2014 03:07:40 GMT

←Older revision		Revision as of 03:07, 4 September 2014
Line 8:		Line 8:
	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vvo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vvo RCSB], [http://www.ebi.ac.uk/pdbsum/1vvo PDBsum]</span></td></tr>		<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vvo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vvo RCSB], [http://www.ebi.ac.uk/pdbsum/1vvo PDBsum]</span></td></tr>
	<table>		<table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Maintenance of the correct reading frame on the ribosome is essential for accurate protein synthesis. Here, we report structures of the 70S ribosome bound to frameshift suppressor tRNASufA6 and N1-methylguanosine at position 37 (m1G37) modification-deficient anticodon stem loopPro, both of which cause the ribosome to decode 4 rather than 3 nucleotides, resulting in a +1 reading frame. Our results reveal that decoding at +1 suppressible codons causes suppressor tRNASufA6 to undergo a rearrangement of its 5' stem that destabilizes U32, thereby disrupting the conserved U32-A38 base pair. Unexpectedly, the removal of the m1G37 modification of tRNAPro also disrupts U32-A38 pairing in a structurally analogous manner. The lack of U32-A38 pairing provides a structural correlation between the transition from canonical translation and a +1 reading of the mRNA. Our structures clarify the molecular mechanism behind suppressor tRNA-induced +1 frameshifting and advance our understanding of the role played by the ribosome in maintaining the correct translational reading frame.
		+
		+	Structural insights into +1 frameshifting promoted by expanded or modification-deficient anticodon stem loops.,Maehigashi T, Dunkle JA, Miles SJ, Dunham CM Proc Natl Acad Sci U S A. 2014 Aug 15. pii: 201409436. PMID:25128388<ref>PMID:25128388</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 02:33, 7 August 2014

OCA — Thu, 07 Aug 2014 02:33:49 GMT

←Older revision		Revision as of 02:33, 7 August 2014
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-	'''~~Unreleased structure~~'''	+	==Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome==
-		+	<StructureSection load='1vvo' size='340' side='right' caption='[[1vvo]], [[Resolution\|resolution]] 3.22Å' scene=''>
-	~~The entry~~ 1vvo is ~~ON HOLD until Aug 14 2015~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[1vvo]] is a 31 chain structure with sequence from [http://en.wikipedia.org/wiki/Thermus_thermophilus Thermus thermophilus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VVO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VVO FirstGlance]. <br>
-	~~Authors~~: ~~Maehigashi~~, T., ~~Dunkle~~, J.A., Dunham, C.M.	+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
-		+	<tr><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PPU:PUROMYCIN-5-MONOPHOSPHATE'>PPU</scene></td></tr>
-	~~Description~~: ~~Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the~~ Ribosome	+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1vvl\|1vvl]], [[1vvm\|1vvm]], [[1vvn\|1vvn]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vvo OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vvo RCSB], [http://www.ebi.ac.uk/pdbsum/1vvo PDBsum]</span></td></tr>
		+	<table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Thermus thermophilus]]
		+	[[Category: Dunham, C M.]]
		+	[[Category: Dunkle, J A.]]
		+	[[Category: Maehigashi, T.]]
		+	[[Category: 16]]
		+	[[Category: 23]]
		+	[[Category: 30]]
		+	[[Category: 70]]
		+	[[Category: Protein biosynthesis]]
		+	[[Category: Ribosomal subunit]]
		+	[[Category: Ribosome]]
		+	[[Category: Ribosome]]
		+	[[Category: Rna]]
		+	[[Category: Transfer rna]]
		+	[[Category: Trna]]

OCA at 06:47, 23 July 2014

OCA — Wed, 23 Jul 2014 06:47:47 GMT

←Older revision		Revision as of 06:47, 23 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 1vvo is ON HOLD until ~~Jul 21~~ 2015	+	The entry 1vvo is ON HOLD until Aug 14 2015

	Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.		Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.

	Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome		Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome

OCA at 09:23, 16 July 2014

OCA — Wed, 16 Jul 2014 09:23:06 GMT

←Older revision		Revision as of 09:23, 16 July 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 1vvo is ON HOLD until Jul 15 2015	+	The entry 1vvo is ON HOLD until Jul 21 2015

	Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.		Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.

	Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome		Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome

OCA at 07:19, 28 May 2014

OCA — Wed, 28 May 2014 07:19:43 GMT

←Older revision		Revision as of 07:19, 28 May 2014
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 1vvo is ON HOLD until ~~Paper Publication~~	+	The entry 1vvo is ON HOLD until Jul 15 2015

	Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.		Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.

	Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome		Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome

OCA: Protected "1vvo" [edit=sysop:move=sysop]

OCA — Thu, 01 Aug 2013 03:50:40 GMT

Protected "1vvo" [edit=sysop:move=sysop]

←Older revision

Revision as of 03:50, 1 August 2013

OCA: New page: '''Unreleased structure''' The entry 1vvo is ON HOLD until Paper Publication Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M. Description: Crystal Structure of Frameshift Suppressor...

OCA — Thu, 01 Aug 2013 03:50:39 GMT

New page: '''Unreleased structure''' The entry 1vvo is ON HOLD until Paper Publication Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M. Description: Crystal Structure of Frameshift Suppressor...

New page

'''Unreleased structure'''

The entry 1vvo is ON HOLD until Paper Publication

Authors: Maehigashi, T., Dunkle, J.A., Dunham, C.M.

Description: Crystal Structure of Frameshift Suppressor tRNA SufA6 Bound to Codon CCC-U on the Ribosome