1vzu - Revision history

OCA at 13:10, 13 December 2023

OCA — Wed, 13 Dec 2023 13:10:04 GMT

←Older revision		Revision as of 13:10, 13 December 2023
Line 3:		Line 3:
	<StructureSection load='1vzu' size='340' side='right'caption='[[1vzu]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='1vzu' size='340' side='right'caption='[[1vzu]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1vzu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Bovin Bovin~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VZU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1vzu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VZU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=~~UDP~~:~~URIDINE~~-~~5-DIPHOSPHATE~~'>~~UDP~~</scene>, <scene name='pdbligand=~~GAL~~:~~BETA~~-D-~~GALACTOSE~~'>~~GAL~~</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.97Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7o\|1o7o]], [[1o7q\|1o7q]], [[1o7r\|1o7r]], [[1vzt\|1vzt]], [[1vzx\|1vzx]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PRD_900004:beta-lactose'>PRD_900004</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vzu OCA], [https://pdbe.org/1vzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vzu RCSB], [https://www.ebi.ac.uk/pdbsum/1vzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vzu ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vzu OCA], [https://pdbe.org/1vzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vzu RCSB], [https://www.ebi.ac.uk/pdbsum/1vzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vzu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN]] Transfer of galactose from UDP-galactose to an acceptor molecule (R).	+	[https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN] Transfer of galactose from UDP-galactose to an acceptor molecule (R).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bovin~~]]	+	[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]~~	+	[[Category: Acharya KR]]
-	[[Category: Acharya~~, K R~~]]	+	[[Category: Brew K]]
-	[[Category: Brew, K]]	+	[[Category: Deshpande A]]
-	[[Category: Deshpande, A]]	+	[[Category: Natesh R]]
-	[[Category: Natesh, R]]	+	[[Category: Xie Z]]
-	[[Category: Xie, Z]]	+	[[Category: Zhang Y]]
-	[[Category: Zhang, Y]]	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Glycosyltransferase]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:44, 29 September 2021

OCA — Wed, 29 Sep 2021 09:44:45 GMT

←Older revision		Revision as of 09:44, 29 September 2021
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	<StructureSection load='1vzu' size='340' side='right'caption='[[1vzu]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='1vzu' size='340' side='right'caption='[[1vzu]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1vzu]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1VZU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1vzu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1VZU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~GOL~~:~~GLYCEROL~~'>~~GOL~~</scene>, <scene name='pdbligand=~~LAT~~:~~BETA-LACTOSE~~'>~~LAT~~</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene>, <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7o\|1o7o]], [[1o7q\|1o7q]], [[1o7r\|1o7r]], [[1vzt\|1vzt]], [[1vzx\|1vzx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7o\|1o7o]], [[1o7q\|1o7q]], [[1o7r\|1o7r]], [[1vzt\|1vzt]], [[1vzx\|1vzx]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1vzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vzu OCA], [~~http~~://pdbe.org/1vzu PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1vzu RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1vzu PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1vzu ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1vzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vzu OCA], [https://pdbe.org/1vzu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1vzu RCSB], [https://www.ebi.ac.uk/pdbsum/1vzu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1vzu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN]] Transfer of galactose from UDP-galactose to an acceptor molecule (R).	+	[[https://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN]] Transfer of galactose from UDP-galactose to an acceptor molecule (R).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:15, 4 December 2019

OCA — Wed, 04 Dec 2019 11:15:14 GMT

←Older revision		Revision as of 11:15, 4 December 2019
Line 1:		Line 1:

	==Roles of active site tryptophans in substrate binding and catalysis by ALPHA-1,3 GALACTOSYLTRANSFERASE==		==Roles of active site tryptophans in substrate binding and catalysis by ALPHA-1,3 GALACTOSYLTRANSFERASE==
-	<StructureSection load='1vzu' size='340' side='right' caption='[[1vzu]], [[Resolution\|resolution]] 1.97Å' scene=''>	+	<StructureSection load='1vzu' size='340' side='right'caption='[[1vzu]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1vzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VZU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1vzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VZU FirstGlance]. <br>
Line 30:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1vzu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1vzu" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glycosyltransferase 3D structures\|Glycosyltransferase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 35:		Line 38:
	</StructureSection>		</StructureSection>
	[[Category: Bovin]]		[[Category: Bovin]]
		+	[[Category: Large Structures]]
	[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]		[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]
	[[Category: Acharya, K R]]		[[Category: Acharya, K R]]

OCA at 06:51, 28 February 2018

OCA — Wed, 28 Feb 2018 06:51:50 GMT

←Older revision		Revision as of 06:51, 28 February 2018
Line 1:		Line 1:
-	==~~ROLES OF ACTIVE SITE TRYPTOPHANS IN SUBSTRATE BINDING AND CATALYSIS BY~~ ALPHA-1,3 GALACTOSYLTRANSFERASE==	+
		+	==Roles of active site tryptophans in substrate binding and catalysis by ALPHA-1,3 GALACTOSYLTRANSFERASE==
	<StructureSection load='1vzu' size='340' side='right' caption='[[1vzu]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='1vzu' size='340' side='right' caption='[[1vzu]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7o\|1o7o]], [[1o7q\|1o7q]], [[1o7r\|1o7r]], [[1vzt\|1vzt]], [[1vzx\|1vzx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7o\|1o7o]], [[1o7q\|1o7q]], [[1o7r\|1o7r]], [[1vzt\|1vzt]], [[1vzx\|1vzx]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vzu OCA], [http://pdbe.org/1vzu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vzu RCSB], [http://www.ebi.ac.uk/pdbsum/1vzu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vzu OCA], [http://pdbe.org/1vzu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vzu RCSB], [http://www.ebi.ac.uk/pdbsum/1vzu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1vzu ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 14:		Line 15:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vz/1vzu_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vz/1vzu_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 29:		Line 30:
	</div>		</div>
	<div class="pdbe-citations 1vzu" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1vzu" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Glycosyltransferase\|Glycosyltransferase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:25, 9 February 2016

OCA — Tue, 09 Feb 2016 07:25:40 GMT

←Older revision		Revision as of 07:25, 9 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1vzu ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 19:57, 10 September 2015

OCA — Thu, 10 Sep 2015 19:57:10 GMT

←Older revision		Revision as of 19:57, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1vzu' size='340' side='right' caption='[[1vzu]], [[Resolution\|resolution]] 1.97Å' scene=''>		<StructureSection load='1vzu' size='340' side='right' caption='[[1vzu]], [[Resolution\|resolution]] 1.97Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1vzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Bos_taurus Bos taurus~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VZU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1vzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovin Bovin]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VZU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LAT:BETA-LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LAT:BETA-LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7o\|1o7o]], [[1o7q\|1o7q]], [[1o7r\|1o7r]], [[1vzt\|1vzt]], [[1vzx\|1vzx]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7o\|1o7o]], [[1o7q\|1o7q]], [[1o7r\|1o7r]], [[1vzt\|1vzt]], [[1vzx\|1vzx]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vzu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vzu RCSB], [http://www.ebi.ac.uk/pdbsum/1vzu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vzu OCA], [http://pdbe.org/1vzu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vzu RCSB], [http://www.ebi.ac.uk/pdbsum/1vzu PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GGTA1_BOVIN GGTA1_BOVIN]] Transfer of galactose from UDP-galactose to an acceptor molecule (R).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 26:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1vzu" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 33:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bos taurus~~]]	+	[[Category: Bovin]]
	[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]		[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]
	[[Category: Acharya, K R]]		[[Category: Acharya, K R]]

OCA at 13:25, 6 January 2015

OCA — Tue, 06 Jan 2015 13:25:54 GMT

←Older revision		Revision as of 13:25, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1vzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VZU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1vzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VZU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LAT:BETA-LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LAT:BETA-LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7o\|1o7o]], [[1o7q\|1o7q]], [[1o7r\|1o7r]], [[1vzt\|1vzt]], [[1vzx\|1vzx]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7o\|1o7o]], [[1o7q\|1o7q]], [[1o7r\|1o7r]], [[1vzt\|1vzt]], [[1vzx\|1vzx]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vzu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vzu RCSB], [http://www.ebi.ac.uk/pdbsum/1vzu PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vzu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vzu RCSB], [http://www.ebi.ac.uk/pdbsum/1vzu PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 35:
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]		[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]
-	[[Category: Acharya, K R.]]	+	[[Category: Acharya, K R]]
-	[[Category: Brew, K.]]	+	[[Category: Brew, K]]
-	[[Category: Deshpande, A.]]	+	[[Category: Deshpande, A]]
-	[[Category: Natesh, R.]]	+	[[Category: Natesh, R]]
-	[[Category: Xie, Z.]]	+	[[Category: Xie, Z]]
-	[[Category: Zhang, Y.]]	+	[[Category: Zhang, Y]]
	[[Category: Glycoprotein]]		[[Category: Glycoprotein]]
	[[Category: Glycosyltransferase]]		[[Category: Glycosyltransferase]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 22:44, 28 September 2014

OCA — Sun, 28 Sep 2014 22:44:12 GMT

←Older revision		Revision as of 22:44, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1vzu.~~png~~\|~~left~~\|200px]]	+	==ROLES OF ACTIVE SITE TRYPTOPHANS IN SUBSTRATE BINDING AND CATALYSIS BY ALPHA-1,3 GALACTOSYLTRANSFERASE==
		+	<StructureSection load='1vzu' size='340' side='right' caption='[[1vzu]], [[Resolution\|resolution]] 1.97Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1vzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1VZU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=LAT:BETA-LACTOSE'>LAT</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1fg5\|1fg5]], [[1g8o\|1g8o]], [[1g93\|1g93]], [[1gwv\|1gwv]], [[1gww\|1gww]], [[1gx0\|1gx0]], [[1gx4\|1gx4]], [[1k4v\|1k4v]], [[1o7o\|1o7o]], [[1o7q\|1o7q]], [[1o7r\|1o7r]], [[1vzt\|1vzt]], [[1vzx\|1vzx]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/N-acetyllactosaminide_3-alpha-galactosyltransferase N-acetyllactosaminide 3-alpha-galactosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.87 2.4.1.87] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vzu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vzu OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1vzu RCSB], [http://www.ebi.ac.uk/pdbsum/1vzu PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vz/1vzu_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Aromatic amino acids are frequent components of the carbohydrate binding sites of lectins and enzymes. Previous structural studies have shown that in alpha-1,3 galactosyltransferase, the binding site for disaccharide acceptor substrates is encircled by four tryptophans, residues 249, 250, 314, and 356. To investigate their roles in enzyme specificity and catalysis, we expressed and characterized variants of the catalytic domain of alpha-1,3 galactosyltransferase with substitutions for each tryptophan. Substitution of glycine for tryptophan 249, whose indole ring interacts with the nonpolar B face of glucose or GlcNAc, greatly increases the K(m) for the acceptor substrate. In contrast, the substitution of tyrosine for tryptophan 314, which interacts with the beta-galactosyl moiety of the acceptor and UDP-galactose, decreases k(cat) for the galactosyltransferase reaction but does not affect the low UDP-galactose hydrolase activity. Thus, this highly conserved residue stabilizes the transition state for the galactose transfer to disaccharide but not to water. High-resolution crystallographic structures of the Trp(249)Gly mutant and the Trp(314)Tyr mutant indicate that the mutations do not affect the overall structure of the enzyme or its interactions with ligands. Substitutions for tryptophan 250 have only small effects on catalytic activity, but mutation of tryptophan 356 to threonine reduces catalytic activity for both transferase and hydrolase activities and reduces affinity for the acceptor substrate. This residue is adjacent to the flexible C-terminus that becomes ordered on binding UDP to assemble the acceptor binding site and influence catalysis. The results highlight the diverse roles of these tryptophans in enzyme action and the importance of k(cat) changes in modulating glycosyltransferase specificity.

-	~~{{STRUCTURE_1vzu\| PDB=1vzu \| SCENE= }}~~	+	Roles of active site tryptophans in substrate binding and catalysis by alpha-1,3 galactosyltransferase.,Zhang Y, Deshpande A, Xie Z, Natesh R, Acharya KR, Brew K Glycobiology. 2004 Dec;14(12):1295-302. Epub 2004 Jun 30. PMID:15229192<ref>PMID:15229192</ref>

-	~~===ROLES OF ACTIVE SITE TRYPTOPHANS IN SUBSTRATE BINDING AND CATALYSIS BY ALPHA-1~~,~~3 GALACTOSYLTRANSFERASE===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15229192}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1vzu]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Bos_taurus Bos taurus]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA].~~	+

	==See Also==		==See Also==
-	*[[~~Galactosyltransferase~~\|~~Galactosyltransferase~~]]	+	*[[Glycosyltransferase\|Glycosyltransferase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015229192<~~/~~ref~~><~~ref group="xtra">PMID:014621997</ref><ref group="xtra">PMID:011179209</ref><ref group="xtra">PMID:011592969</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]		[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]

OCA at 13:06, 20 October 2012

OCA — Sat, 20 Oct 2012 13:06:35 GMT

←Older revision		Revision as of 13:06, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1vzu]] is a 2 chain structure ~~of [[Galactosyltransferase]]~~ with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA].	+	[[1vzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA].

	==See Also==		==See Also==

OCA at 10:55, 26 July 2012

OCA — Thu, 26 Jul 2012 10:55:53 GMT

←Older revision		Revision as of 10:55, 26 July 2012
Line 1:		Line 1:
	[[Image:1vzu.png\|left\|200px]]		[[Image:1vzu.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1vzu", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1vzu\| PDB=1vzu \| SCENE= }}		{{STRUCTURE_1vzu\| PDB=1vzu \| SCENE= }}

	===ROLES OF ACTIVE SITE TRYPTOPHANS IN SUBSTRATE BINDING AND CATALYSIS BY ALPHA-1,3 GALACTOSYLTRANSFERASE===		===ROLES OF ACTIVE SITE TRYPTOPHANS IN SUBSTRATE BINDING AND CATALYSIS BY ALPHA-1,3 GALACTOSYLTRANSFERASE===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15229192}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15229192 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15229192}}		{{ABSTRACT_PUBMED_15229192}}

	==About this Structure==		==About this Structure==
-	[[1vzu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA].	+	[[1vzu]] is a 2 chain structure of [[Galactosyltransferase]] with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VZU OCA].
		+
		+	==See Also==
		+	*[[Galactosyltransferase\|Galactosyltransferase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15229192~~</ref><ref group="xtra">PMID:~~14621997~~</ref><ref group="xtra">PMID:~~11179209~~</ref><ref group="xtra">PMID:~~11592969~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015229192</ref><ref group="xtra">PMID:014621997</ref><ref group="xtra">PMID:011179209</ref><ref group="xtra">PMID:011592969</ref><references group="xtra"/>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]		[[Category: N-acetyllactosaminide 3-alpha-galactosyltransferase]]