1w5n - Revision history

OCA at 13:16, 13 December 2023

OCA — Wed, 13 Dec 2023 13:16:56 GMT

←Older revision		Revision as of 13:16, 13 December 2023
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	<StructureSection load='1w5n' size='340' side='right'caption='[[1w5n]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1w5n' size='340' side='right'caption='[[1w5n]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1w5n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W5N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1w5n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W5N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1b4k\|1b4k]], [[1gzg\|1gzg]], [[1w54\|1w54]], [[1w56\|1w56]], [[1w5m\|1w5m]], [[1w5o\|1w5o]], [[1w5p\|1w5p]], [[1w5q\|1w5q]]</div></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w5n OCA], [https://pdbe.org/1w5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w5n RCSB], [https://www.ebi.ac.uk/pdbsum/1w5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w5n ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w5n OCA], [https://pdbe.org/1w5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w5n RCSB], [https://www.ebi.ac.uk/pdbsum/1w5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w5n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HEM2_PSEAE HEM2_PSEAE]] Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).	+	[https://www.uniprot.org/uniprot/HEM2_PSEAE HEM2_PSEAE] Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Porphobilinogen synthase]]~~	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	~~[[Category: Pseae~~]]	+	[[Category: Frere F]]
-	[[Category: Frere, F]]	+	[[Category: Heinz DW]]
-	[[Category: Heinz~~, D W~~]]	+	[[Category: Jahn D]]
-	[[Category: Jahn, D]]	+	[[Category: Reents H]]
-	[[Category: Reents, H]]	+	[[Category: Schubert W-D]]
-	[[Category: Schubert, W D]]	+
-	~~[[Category: Evolution]]~~	+
-	~~[[Category: Metalloenzyme]]~~	+
-	~~[[Category: Protein engineering]]~~	+
-	~~[[Category: Pseudomonas aeruginosa]]~~	+
-	~~[[Category: Synthase~~]]	+

OCA at 10:30, 17 February 2021

OCA — Wed, 17 Feb 2021 10:30:24 GMT

←Older revision		Revision as of 10:30, 17 February 2021
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	<StructureSection load='1w5n' size='340' side='right'caption='[[1w5n]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1w5n' size='340' side='right'caption='[[1w5n]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1w5n]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1W5N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1w5n]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1W5N FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b4k\|1b4k]], [[1gzg\|1gzg]], [[1w54\|1w54]], [[1w56\|1w56]], [[1w5m\|1w5m]], [[1w5o\|1w5o]], [[1w5p\|1w5p]], [[1w5q\|1w5q]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1b4k\|1b4k]], [[1gzg\|1gzg]], [[1w54\|1w54]], [[1w56\|1w56]], [[1w5m\|1w5m]], [[1w5o\|1w5o]], [[1w5p\|1w5p]], [[1w5q\|1w5q]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1w5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w5n OCA], [~~http~~://pdbe.org/1w5n PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1w5n RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1w5n PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1w5n ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1w5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w5n OCA], [https://pdbe.org/1w5n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1w5n RCSB], [https://www.ebi.ac.uk/pdbsum/1w5n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1w5n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HEM2_PSEAE HEM2_PSEAE]] Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).	+	[[https://www.uniprot.org/uniprot/HEM2_PSEAE HEM2_PSEAE]] Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 11:48, 10 May 2019

OCA — Fri, 10 May 2019 11:48:05 GMT

←Older revision		Revision as of 11:48, 10 May 2019
Line 1:		Line 1:

	==Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations D131C and D139C)==		==Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations D131C and D139C)==
-	<StructureSection load='1w5n' size='340' side='right' caption='[[1w5n]], [[Resolution\|resolution]] 1.65Å' scene=''>	+	<StructureSection load='1w5n' size='340' side='right'caption='[[1w5n]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1w5n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W5N FirstGlance]. <br>		<table><tr><td colspan='2'>[[1w5n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W5N FirstGlance]. <br>
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Large Structures]]
	[[Category: Porphobilinogen synthase]]		[[Category: Porphobilinogen synthase]]
	[[Category: Pseae]]		[[Category: Pseae]]

OCA at 07:32, 4 April 2018

OCA — Wed, 04 Apr 2018 07:32:10 GMT

←Older revision		Revision as of 07:32, 4 April 2018
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-	==~~STEPWISE INTRODUCTION OF ZINC BINDING SITE INTO PORPHOBILINOGEN SYNTHASE OF PSEUDOMONAS AERUGINOSA~~ (~~MUTATIONS~~ D131C ~~AND~~ D139C)==	+
		+	==Stepwise introduction of zinc binding site into porphobilinogen synthase of Pseudomonas aeruginosa (mutations D131C and D139C)==
	<StructureSection load='1w5n' size='340' side='right' caption='[[1w5n]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1w5n' size='340' side='right' caption='[[1w5n]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b4k\|1b4k]], [[1gzg\|1gzg]], [[1w54\|1w54]], [[1w56\|1w56]], [[1w5m\|1w5m]], [[1w5o\|1w5o]], [[1w5p\|1w5p]], [[1w5q\|1w5q]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b4k\|1b4k]], [[1gzg\|1gzg]], [[1w54\|1w54]], [[1w56\|1w56]], [[1w5m\|1w5m]], [[1w5o\|1w5o]], [[1w5p\|1w5p]], [[1w5q\|1w5q]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w5n OCA], [http://pdbe.org/1w5n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1w5n RCSB], [http://www.ebi.ac.uk/pdbsum/1w5n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w5n OCA], [http://pdbe.org/1w5n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1w5n RCSB], [http://www.ebi.ac.uk/pdbsum/1w5n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1w5n ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/1w5n_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/1w5n_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 01:37, 10 February 2016

OCA — Wed, 10 Feb 2016 01:37:55 GMT

←Older revision		Revision as of 01:37, 10 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1w5n ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 04:05, 11 September 2015

OCA — Fri, 11 Sep 2015 04:05:11 GMT

←Older revision		Revision as of 04:05, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1w5n' size='340' side='right' caption='[[1w5n]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='1w5n' size='340' side='right' caption='[[1w5n]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1w5n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Pseudomonas_aeruginosa Pseudomonas aeruginosa~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W5N FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1w5n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W5N FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b4k\|1b4k]], [[1gzg\|1gzg]], [[1w54\|1w54]], [[1w56\|1w56]], [[1w5m\|1w5m]], [[1w5o\|1w5o]], [[1w5p\|1w5p]], [[1w5q\|1w5q]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b4k\|1b4k]], [[1gzg\|1gzg]], [[1w54\|1w54]], [[1w56\|1w56]], [[1w5m\|1w5m]], [[1w5o\|1w5o]], [[1w5p\|1w5p]], [[1w5q\|1w5q]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w5n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1w5n RCSB], [http://www.ebi.ac.uk/pdbsum/1w5n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w5n OCA], [http://pdbe.org/1w5n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1w5n RCSB], [http://www.ebi.ac.uk/pdbsum/1w5n PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1w5n" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	</StructureSection>		</StructureSection>
	[[Category: Porphobilinogen synthase]]		[[Category: Porphobilinogen synthase]]
-	[[Category: ~~Pseudomonas aeruginosa~~]]	+	[[Category: Pseae]]
	[[Category: Frere, F]]		[[Category: Frere, F]]
	[[Category: Heinz, D W]]		[[Category: Heinz, D W]]
Line 45:		Line 46:
	[[Category: Metalloenzyme]]		[[Category: Metalloenzyme]]
	[[Category: Protein engineering]]		[[Category: Protein engineering]]
		+	[[Category: Pseudomonas aeruginosa]]
	[[Category: Synthase]]		[[Category: Synthase]]

OCA at 10:54, 25 December 2014

OCA — Thu, 25 Dec 2014 10:54:10 GMT

←Older revision		Revision as of 10:54, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1w5n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W5N FirstGlance]. <br>		<table><tr><td colspan='2'>[[1w5n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W5N FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b4k\|1b4k]], [[1gzg\|1gzg]], [[1w54\|1w54]], [[1w56\|1w56]], [[1w5m\|1w5m]], [[1w5o\|1w5o]], [[1w5p\|1w5p]], [[1w5q\|1w5q]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b4k\|1b4k]], [[1gzg\|1gzg]], [[1w54\|1w54]], [[1w56\|1w56]], [[1w5m\|1w5m]], [[1w5o\|1w5o]], [[1w5p\|1w5p]], [[1w5q\|1w5q]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w5n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1w5n RCSB], [http://www.ebi.ac.uk/pdbsum/1w5n PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w5n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1w5n RCSB], [http://www.ebi.ac.uk/pdbsum/1w5n PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HEM2_PSEAE HEM2_PSEAE]] Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Porphobilinogen synthase]]		[[Category: Porphobilinogen synthase]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
-	[[Category: Frere, F.]]	+	[[Category: Frere, F]]
-	[[Category: Heinz, D W.]]	+	[[Category: Heinz, D W]]
-	[[Category: Jahn, D.]]	+	[[Category: Jahn, D]]
-	[[Category: Reents, H.]]	+	[[Category: Reents, H]]
-	[[Category: Schubert, W D.]]	+	[[Category: Schubert, W D]]
	[[Category: Evolution]]		[[Category: Evolution]]
	[[Category: Metalloenzyme]]		[[Category: Metalloenzyme]]
-	[[Category: Porphobilinogen synthase]]
	[[Category: Protein engineering]]		[[Category: Protein engineering]]
-	[[Category: Pseudomonas aeruginosa]]
	[[Category: Synthase]]		[[Category: Synthase]]

OCA at 21:55, 29 September 2014

OCA — Mon, 29 Sep 2014 21:55:37 GMT

←Older revision		Revision as of 21:55, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1w5n.~~png~~\|~~left~~\|200px]]	+	==STEPWISE INTRODUCTION OF ZINC BINDING SITE INTO PORPHOBILINOGEN SYNTHASE OF PSEUDOMONAS AERUGINOSA (MUTATIONS D131C AND D139C)==
		+	<StructureSection load='1w5n' size='340' side='right' caption='[[1w5n]], [[Resolution\|resolution]] 1.65Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1w5n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1W5N FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1b4k\|1b4k]], [[1gzg\|1gzg]], [[1w54\|1w54]], [[1w56\|1w56]], [[1w5m\|1w5m]], [[1w5o\|1w5o]], [[1w5p\|1w5p]], [[1w5q\|1w5q]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Porphobilinogen_synthase Porphobilinogen synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.24 4.2.1.24] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1w5n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1w5n OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1w5n RCSB], [http://www.ebi.ac.uk/pdbsum/1w5n PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/1w5n_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Metal ions are indispensable cofactors for chemical catalysis by a plethora of enzymes. Porphobilinogen synthases (PBGSs), which catalyse the second step of tetrapyrrole biosynthesis, are grouped according to their dependence on Zn(2+). Using site-directed mutagenesis, we embarked on transforming Zn(2+)-independent Pseudomonas aeruginosa PBGS into a Zn(2+)-dependent enzyme. Nine PBGS variants were generated by permutationally introducing three cysteine residues and a further two residues into the active site of the enzyme to match the homologous Zn(2+)-containing PBGS from Escherichia coli. Crystal structures of seven enzyme variants were solved to elucidate the nature of Zn(2+) coordination at high resolution. The three single-cysteine variants were invariably found to be enzymatically inactive and only one (D139C) was found to bind detectable amounts of Zn(2+). The double mutant A129C/D139C is enzymatically active and binds Zn(2+) in a tetrahedral coordination. Structurally and functionally it mimics mycobacterial PBGS, which bears an equivalent Zn(2+)-coordination site. The remaining two double mutants, without known natural equivalents, reveal strongly distorted tetrahedral Zn(2+)-binding sites. Variant A129C/D131C possesses weak PBGS activity while D131C/D139C is inactive. The triple mutant A129C/D131C/D139C, finally, displays an almost ideal tetrahedral Zn(2+)-binding geometry and a significant Zn(2+)-dependent enzymatic activity. Two additional amino acid exchanges further optimize the active site architecture towards the E.coli enzyme with an additional increase in activity. Our study delineates the potential evolutionary path between Zn(2+)-free and Zn(2+)-dependent PBGS enyzmes showing that the rigid backbone of PBGS enzymes is an ideal framework to create or eliminate metal dependence through a limited number of amino acid exchanges.

-	~~{{STRUCTURE_1w5n\| PDB=1w5n \| SCENE= }}~~	+	Tracking the evolution of porphobilinogen synthase metal dependence in vitro.,Frere F, Reents H, Schubert WD, Heinz DW, Jahn D J Mol Biol. 2005 Feb 4;345(5):1059-70. Epub 2004 Dec 21. PMID:15644204<ref>PMID:15644204</ref>

-	~~===STEPWISE INTRODUCTION OF ZINC BINDING SITE INTO PORPHOBILINOGEN SYNTHASE OF PSEUDOMONAS AERUGINOSA (MUTATIONS D131C AND D139C)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15644204}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1w5n]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA].~~	+

	==See Also==		==See Also==
	*[[Porphobilinogen synthase\|Porphobilinogen synthase]]		*[[Porphobilinogen synthase\|Porphobilinogen synthase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015644204<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Porphobilinogen synthase]]		[[Category: Porphobilinogen synthase]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]

OCA: Protected "1w5n" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 13:31:27 GMT

Protected "1w5n" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:31, 5 January 2013

OCA at 13:31, 5 January 2013

OCA — Sat, 05 Jan 2013 13:31:23 GMT

←Older revision		Revision as of 13:31, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1w5n.png\|left\|200px]]		[[Image:1w5n.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1w5n", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1w5n\| PDB=1w5n \| SCENE= }}		{{STRUCTURE_1w5n\| PDB=1w5n \| SCENE= }}

	===STEPWISE INTRODUCTION OF ZINC BINDING SITE INTO PORPHOBILINOGEN SYNTHASE OF PSEUDOMONAS AERUGINOSA (MUTATIONS D131C AND D139C)===		===STEPWISE INTRODUCTION OF ZINC BINDING SITE INTO PORPHOBILINOGEN SYNTHASE OF PSEUDOMONAS AERUGINOSA (MUTATIONS D131C AND D139C)===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15644204}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15644204 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15644204}}		{{ABSTRACT_PUBMED_15644204}}

	==About this Structure==		==About this Structure==
-	~~1W5N~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA].	+	[[1w5n]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1W5N OCA].
		+
		+	==See Also==
		+	*[[Porphobilinogen synthase\|Porphobilinogen synthase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15644204~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015644204</ref><references group="xtra"/>
	[[Category: Porphobilinogen synthase]]		[[Category: Porphobilinogen synthase]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
Line 36:		Line 27:
	[[Category: Protein engineering]]		[[Category: Protein engineering]]
	[[Category: Pseudomonas aeruginosa]]		[[Category: Pseudomonas aeruginosa]]
-		+	[[Category: Synthase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Tue Feb 17 14:22:23 2009''~~	+