1wau - Revision history

OCA at 13:26, 13 December 2023

OCA — Wed, 13 Dec 2023 13:26:15 GMT

←Older revision		Revision as of 13:26, 13 December 2023
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	<StructureSection load='1wau' size='340' side='right'caption='[[1wau]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1wau' size='340' side='right'caption='[[1wau]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wau]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WAU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wau]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WAU FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.8Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wau OCA], [https://pdbe.org/1wau PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wau RCSB], [https://www.ebi.ac.uk/pdbsum/1wau PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wau ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wau OCA], [https://pdbe.org/1wau PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wau RCSB], [https://www.ebi.ac.uk/pdbsum/1wau PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wau ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ALKH_ECOLI ALKH_ECOLI]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillus coli migula 1895]]
-	[[Category: Large Structures]]
-	[[Category: Merkel, A B]]
-	[[Category: Naismith, J H]]
-	[[Category: E45n mutant]]
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: ~~Kdpg aldolase~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Lyase~~]]	+	[[Category: Merkel AB]]
-	[[Category: ~~Multifunctional enzyme~~]]	+	[[Category: Naismith JH]]

OCA at 09:57, 26 May 2021

OCA — Wed, 26 May 2021 09:57:36 GMT

←Older revision		Revision as of 09:57, 26 May 2021
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	<StructureSection load='1wau' size='340' side='right'caption='[[1wau]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1wau' size='340' side='right'caption='[[1wau]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wau]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1WAU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wau]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WAU FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1wau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wau OCA], [~~http~~://pdbe.org/1wau PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1wau RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1wau PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1wau ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wau OCA], [https://pdbe.org/1wau PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wau RCSB], [https://www.ebi.ac.uk/pdbsum/1wau PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wau ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 07:20, 21 August 2019

OCA — Wed, 21 Aug 2019 07:20:37 GMT

←Older revision		Revision as of 07:20, 21 August 2019
Line 1:		Line 1:

	==Structure of KDPG Aldolase E45N mutant==		==Structure of KDPG Aldolase E45N mutant==
-	<StructureSection load='1wau' size='340' side='right' caption='[[1wau]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<StructureSection load='1wau' size='340' side='right'caption='[[1wau]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wau]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WAU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wau]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WAU FirstGlance]. <br>
Line 12:		Line 12:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/1wau_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/1wau_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 27:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1wau" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1wau" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Aldolase 3D structures\|Aldolase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 32:		Line 35:
	</StructureSection>		</StructureSection>
	[[Category: Bacillus coli migula 1895]]		[[Category: Bacillus coli migula 1895]]
		+	[[Category: Large Structures]]
	[[Category: Merkel, A B]]		[[Category: Merkel, A B]]
	[[Category: Naismith, J H]]		[[Category: Naismith, J H]]

OCA at 07:11, 16 November 2017

OCA — Thu, 16 Nov 2017 07:11:27 GMT

←Older revision		Revision as of 07:11, 16 November 2017
Line 1:		Line 1:
-	==~~STRUCTURE OF~~ KDPG ~~ALDOLASE~~ E45N ~~MUTANT~~==	+
		+	==Structure of KDPG Aldolase E45N mutant==
	<StructureSection load='1wau' size='340' side='right' caption='[[1wau]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1wau' size='340' side='right' caption='[[1wau]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
Line 5:		Line 6:
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wau OCA], [http://pdbe.org/1wau PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wau RCSB], [http://www.ebi.ac.uk/pdbsum/1wau PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wau OCA], [http://pdbe.org/1wau PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wau RCSB], [http://www.ebi.ac.uk/pdbsum/1wau PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wau ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 26:		Line 27:
	</div>		</div>
	<div class="pdbe-citations 1wau" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1wau" style="background-color:#fffaf0;"></div>
-
-	==See Also==
-	*[[Aldolase\|Aldolase]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 10:46, 7 February 2016

OCA — Sun, 07 Feb 2016 10:46:21 GMT

←Older revision		Revision as of 10:46, 7 February 2016
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wau ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 00:46, 10 September 2015

OCA — Thu, 10 Sep 2015 00:46:45 GMT

←Older revision		Revision as of 00:46, 10 September 2015
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	<StructureSection load='1wau' size='340' side='right' caption='[[1wau]], [[Resolution\|resolution]] 2.80Å' scene=''>		<StructureSection load='1wau' size='340' side='right' caption='[[1wau]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wau]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Escherichia_coli Escherichia~~ coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WAU FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wau]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WAU FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wau OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wau RCSB], [http://www.ebi.ac.uk/pdbsum/1wau PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wau OCA], [http://pdbe.org/1wau PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wau RCSB], [http://www.ebi.ac.uk/pdbsum/1wau PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 25:		Line 25:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1wau" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 32:		Line 33:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Escherichia~~ coli]]	+	[[Category: Bacillus coli migula 1895]]
	[[Category: Merkel, A B]]		[[Category: Merkel, A B]]
	[[Category: Naismith, J H]]		[[Category: Naismith, J H]]
	[[Category: E45n mutant]]		[[Category: E45n mutant]]
		+	[[Category: Escherichia coli]]
	[[Category: Kdpg aldolase]]		[[Category: Kdpg aldolase]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Multifunctional enzyme]]		[[Category: Multifunctional enzyme]]

OCA at 12:36, 6 January 2015

OCA — Tue, 06 Jan 2015 12:36:38 GMT

←Older revision		Revision as of 12:36, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wau]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WAU FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wau]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WAU FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wau OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wau RCSB], [http://www.ebi.ac.uk/pdbsum/1wau PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wau OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wau RCSB], [http://www.ebi.ac.uk/pdbsum/1wau PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Merkel, A B.]]	+	[[Category: Merkel, A B]]
-	[[Category: Naismith, J H.]]	+	[[Category: Naismith, J H]]
	[[Category: E45n mutant]]		[[Category: E45n mutant]]
-	[[Category: Escherichia coli]]
	[[Category: Kdpg aldolase]]		[[Category: Kdpg aldolase]]
	[[Category: Lyase]]		[[Category: Lyase]]
	[[Category: Multifunctional enzyme]]		[[Category: Multifunctional enzyme]]

OCA at 22:20, 28 September 2014

OCA — Sun, 28 Sep 2014 22:20:16 GMT

←Older revision		Revision as of 22:20, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1wau.~~png~~\|~~left~~\|200px]]	+	==STRUCTURE OF KDPG ALDOLASE E45N MUTANT==
		+	<StructureSection load='1wau' size='340' side='right' caption='[[1wau]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1wau]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WAU FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1eua\|1eua]], [[1eun\|1eun]], [[1fq0\|1fq0]], [[1fwr\|1fwr]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wau FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wau OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wau RCSB], [http://www.ebi.ac.uk/pdbsum/1wau PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wa/1wau_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In vivo, 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase catalyzes the reversible, stereospecific retro-aldol cleavage of KDPG to pyruvate and D-glyceraldehyde-3-phosphate. The enzyme is a lysine-dependent (Class I) aldolase that functions through the intermediacy of a Schiff base. Here, we propose a mechanism for this enzyme based on crystallographic studies of wild-type and mutant aldolases. The three dimensional structure of KDPG aldolase from the thermophile Thermotoga maritima was determined to 1.9A. The structure is the standard alpha/beta barrel observed for all Class I aldolases. At the active site Lys we observe clear density for a pyruvate Schiff base. Density for a sulfate ion bound in a conserved cluster of residues close to the Schiff base is also observed. We have also determined the structure of a mutant of Escherichia coli KDPG aldolase in which the proposed general acid/base catalyst has been removed (E45N). One subunit of the trimer contains density suggesting a trapped pyruvate carbinolamine intermediate. All three subunits contain a phosphate ion bound in a location effectively identical to that of the sulfate ion bound in the T. maritima enzyme. The sulfate and phosphate ions experimentally locate the putative phosphate binding site of the aldolase and, together with the position of the bound pyruvate, facilitate construction of a model for the full-length KDPG substrate complex. The model requires only minimal positional adjustments of the experimentally determined covalent intermediate and bound anion to accommodate full-length substrate. The model identifies the key catalytic residues of the protein and suggests important roles for two observable water molecules. The first water molecule remains bound to the enzyme during the entire catalytic cycle, shuttling protons between the catalytic glutamate and the substrate. The second water molecule arises from dehydration of the carbinolamine and serves as the nucleophilic water during hydrolysis of the enzyme-product Schiff base. The second water molecule may also mediate the base-catalyzed enolization required to form the carbon nucleophile, again bridging to the catalytic glutamate. Many aspects of this mechanism are observed in other Class I aldolases and suggest a mechanistically and, perhaps, evolutionarily related family of aldolases distinct from the N-acetylneuraminate lyase (NAL) family.

-	~~{{STRUCTURE_1wau\| PDB=1wau \| SCENE= }}~~	+	Mechanism of the Class I KDPG aldolase.,Fullerton SW, Griffiths JS, Merkel AB, Cheriyan M, Wymer NJ, Hutchins MJ, Fierke CA, Toone EJ, Naismith JH Bioorg Med Chem. 2006 May 1;14(9):3002-10. Epub 2006 Jan 5. PMID:16403639<ref>PMID:16403639</ref>

-	~~===STRUCTURE OF KDPG ALDOLASE E45N MUTANT===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_16403639}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1wau]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA].~~	+

	==See Also==		==See Also==
	*[[Aldolase\|Aldolase]]		*[[Aldolase\|Aldolase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:016403639<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Merkel, A B.]]		[[Category: Merkel, A B.]]

OCA at 06:24, 20 October 2012

OCA — Sat, 20 Oct 2012 06:24:25 GMT

←Older revision		Revision as of 06:24, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1wau]] is a 1 chain structure ~~of [[Aldolase]]~~ with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA].	+	[[1wau]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WAU OCA].

	==See Also==		==See Also==

OCA: Protected "1wau" [edit=sysop:move=sysop]

OCA — Wed, 25 Jul 2012 13:05:23 GMT

Protected "1wau" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:05, 25 July 2012