1wdf - Revision history

OCA at 13:31, 13 March 2024

OCA — Wed, 13 Mar 2024 13:31:23 GMT

←Older revision		Revision as of 13:31, 13 March 2024
Line 3:		Line 3:
	<StructureSection load='1wdf' size='340' side='right'caption='[[1wdf]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1wdf' size='340' side='right'caption='[[1wdf]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Cvma5 Cvma5~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WDF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Murine_hepatitis_virus_strain_A59 Murine hepatitis virus strain A59]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WDF FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>~~[[~~1wdg~~\|~~1wdg~~]]~~</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.5Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdf OCA], [https://pdbe.org/1wdf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wdf RCSB], [https://www.ebi.ac.uk/pdbsum/1wdf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wdf ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdf OCA], [https://pdbe.org/1wdf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wdf RCSB], [https://www.ebi.ac.uk/pdbsum/1wdf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wdf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/SPIKE_CVMA5 SPIKE_CVMA5]] S1 attaches the virion to the cell membrane by interacting with murine CEACAM1, initiating the infection.<ref>PMID:16014947</ref> S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Presumably interacts with target cell lipid raft after cell attachment.<ref>PMID:16014947</ref>	+	[https://www.uniprot.org/uniprot/SPIKE_CVMA5 SPIKE_CVMA5] S1 attaches the virion to the cell membrane by interacting with murine CEACAM1, initiating the infection.<ref>PMID:16014947</ref> S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Presumably interacts with target cell lipid raft after cell attachment.<ref>PMID:16014947</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wdf ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wdf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The surface transmembrane glycoprotein is responsible for mediating virion attachment to cell and subsequent virus-cell membrane fusion. However, the molecular mechanisms for the viral entry of coronaviruses remain poorly understood. The crystal structure of the fusion core of mouse hepatitis virus S protein, which represents the first fusion core structure of any coronavirus, reveals a central hydrophobic coiled coil trimer surrounded by three helices in an oblique, antiparallel manner. This structure shares significant similarity with both the low pH-induced conformation of influenza hemagglutinin and fusion core of HIV gp41, indicating that the structure represents a fusion-active state formed after several conformational changes. Our results also indicate that the mechanisms for the viral fusion of coronaviruses are similar to those of influenza virus and HIV. The coiled coil structure has unique features, which are different from other viral fusion cores. Highly conserved heptad repeat 1 (HR1) and HR2 regions in coronavirus spike proteins indicate a similar three-dimensional structure among these fusion cores and common mechanisms for the viral fusion. We have proposed the binding regions of HR1 and HR2 of other coronaviruses and a structure model of their fusion core based on our mouse hepatitis virus fusion core structure and sequence alignment. Drug discovery strategies aimed at inhibiting viral entry by blocking hairpin formation may be applied to the inhibition of a number of emerging infectious diseases, including severe acute respiratory syndrome.
-
-	Structural basis for coronavirus-mediated membrane fusion. Crystal structure of mouse hepatitis virus spike protein fusion core.,Xu Y, Liu Y, Lou Z, Qin L, Li X, Bai Z, Pang H, Tien P, Gao GF, Rao Z J Biol Chem. 2004 Jul 16;279(29):30514-22. Epub 2004 Apr 27. PMID:15123674<ref>PMID:15123674</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1wdf" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Sandbox 3001\|Sandbox 3001]]		*[[Sandbox 3001\|Sandbox 3001]]
-	*[[Spike protein\|Spike protein]]	+	*[[Spike protein 3D structures\|Spike protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Cvma5]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bai, Z]]	+	[[Category: Murine hepatitis virus strain A59]]
-	[[Category: Gao~~, G F~~]]	+	[[Category: Bai Z]]
-	[[Category: Li, X]]	+	[[Category: Gao GF]]
-	[[Category: Liu, Y]]	+	[[Category: Li X]]
-	[[Category: Lou, Z]]	+	[[Category: Liu Y]]
-	[[Category: Qin, L]]	+	[[Category: Lou Z]]
-	[[Category: Rao, Z]]	+	[[Category: Qin L]]
-	[[Category: Tien, P]]	+	[[Category: Rao Z]]
-	[[Category: Xu, Y]]	+	[[Category: Tien P]]
-	~~[[Category: Coronavirus]]~~	+	[[Category: Xu Y]]
-	~~[[Category: Fusion core]]~~	+
-	~~[[Category: Heptad repeat]]~~	+
-	~~[[Category: Mhv]]~~	+
-	~~[[Category: Viral entry]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 09:57, 26 May 2021

OCA — Wed, 26 May 2021 09:57:38 GMT

←Older revision		Revision as of 09:57, 26 May 2021
Line 3:		Line 3:
	<StructureSection load='1wdf' size='340' side='right'caption='[[1wdf]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1wdf' size='340' side='right'caption='[[1wdf]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Cvma5 Cvma5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1WDF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cvma5 Cvma5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WDF FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdg\|1wdg]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wdg\|1wdg]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdf OCA], [~~http~~://pdbe.org/1wdf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1wdf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1wdf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1wdf ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdf OCA], [https://pdbe.org/1wdf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wdf RCSB], [https://www.ebi.ac.uk/pdbsum/1wdf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wdf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/SPIKE_CVMA5 SPIKE_CVMA5]] S1 attaches the virion to the cell membrane by interacting with murine CEACAM1, initiating the infection.<ref>PMID:16014947</ref> S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Presumably interacts with target cell lipid raft after cell attachment.<ref>PMID:16014947</ref>	+	[[https://www.uniprot.org/uniprot/SPIKE_CVMA5 SPIKE_CVMA5]] S1 attaches the virion to the cell membrane by interacting with murine CEACAM1, initiating the infection.<ref>PMID:16014947</ref> S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Presumably interacts with target cell lipid raft after cell attachment.<ref>PMID:16014947</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 28:		Line 28:
	</div>		</div>
	<div class="pdbe-citations 1wdf" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 1wdf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Sandbox 3001\|Sandbox 3001]]
		+	*[[Spike protein\|Spike protein]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 07:06, 21 August 2019

OCA — Wed, 21 Aug 2019 07:06:37 GMT

←Older revision		Revision as of 07:06, 21 August 2019
Line 1:		Line 1:

	==crystal structure of MHV spike protein fusion core==		==crystal structure of MHV spike protein fusion core==
-	<StructureSection load='1wdf' size='340' side='right' caption='[[1wdf]], [[Resolution\|resolution]] 2.50Å' scene=''>	+	<StructureSection load='1wdf' size='340' side='right'caption='[[1wdf]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvma5 Cvma5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvma5 Cvma5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDF FirstGlance]. <br>
Line 13:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/1wdf_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/1wdf_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Cvma5]]		[[Category: Cvma5]]
		+	[[Category: Large Structures]]
	[[Category: Bai, Z]]		[[Category: Bai, Z]]
	[[Category: Gao, G F]]		[[Category: Gao, G F]]

OCA at 00:27, 16 November 2017

OCA — Thu, 16 Nov 2017 00:27:57 GMT

←Older revision		Revision as of 00:27, 16 November 2017
Line 1:		Line 1:
		+
	==crystal structure of MHV spike protein fusion core==		==crystal structure of MHV spike protein fusion core==
	<StructureSection load='1wdf' size='340' side='right' caption='[[1wdf]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1wdf' size='340' side='right' caption='[[1wdf]], [[Resolution\|resolution]] 2.50Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvma5 Cvma5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvma5 Cvma5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdg\|1wdg]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdg\|1wdg]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdf OCA], [http://pdbe.org/1wdf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wdf RCSB], [http://www.ebi.ac.uk/pdbsum/1wdf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdf OCA], [http://pdbe.org/1wdf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wdf RCSB], [http://www.ebi.ac.uk/pdbsum/1wdf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wdf ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 17:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wdf ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 17:19, 11 September 2015

OCA — Fri, 11 Sep 2015 17:19:35 GMT

←Older revision		Revision as of 17:19, 11 September 2015
Line 2:		Line 2:
	<StructureSection load='1wdf' size='340' side='right' caption='[[1wdf]], [[Resolution\|resolution]] 2.50Å' scene=''>		<StructureSection load='1wdf' size='340' side='right' caption='[[1wdf]], [[Resolution\|resolution]] 2.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Murine_hepatitis_virus_strain_a59 Murine hepatitis virus strain a59~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cvma5 Cvma5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDF FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdg\|1wdg]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdg\|1wdg]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wdf RCSB], [http://www.ebi.ac.uk/pdbsum/1wdf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdf OCA], [http://pdbe.org/1wdf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wdf RCSB], [http://www.ebi.ac.uk/pdbsum/1wdf PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SPIKE_CVMA5 SPIKE_CVMA5]] S1 attaches the virion to the cell membrane by interacting with murine CEACAM1, initiating the infection.<ref>PMID:16014947</ref> S2 is a class I viral fusion protein. Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state. During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. The formation of this structure appears to drive apposition and subsequent fusion of viral and plasma cell membranes. Presumably interacts with target cell lipid raft after cell attachment.<ref>PMID:16014947</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 24:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1wdf" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Murine hepatitis virus strain a59~~]]	+	[[Category: Cvma5]]
	[[Category: Bai, Z]]		[[Category: Bai, Z]]
	[[Category: Gao, G F]]		[[Category: Gao, G F]]

OCA at 13:21, 6 January 2015

OCA — Tue, 06 Jan 2015 13:21:03 GMT

←Older revision		Revision as of 13:21, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_hepatitis_virus_strain_a59 Murine hepatitis virus strain a59]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDF FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_hepatitis_virus_strain_a59 Murine hepatitis virus strain a59]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDF FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdg\|1wdg]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdg\|1wdg]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wdf RCSB], [http://www.ebi.ac.uk/pdbsum/1wdf PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wdf RCSB], [http://www.ebi.ac.uk/pdbsum/1wdf PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 29:
	</StructureSection>		</StructureSection>
	[[Category: Murine hepatitis virus strain a59]]		[[Category: Murine hepatitis virus strain a59]]
-	[[Category: Bai, Z.]]	+	[[Category: Bai, Z]]
-	[[Category: Gao, G F.]]	+	[[Category: Gao, G F]]
-	[[Category: Li, X.]]	+	[[Category: Li, X]]
-	[[Category: Liu, Y.]]	+	[[Category: Liu, Y]]
-	[[Category: Lou, Z.]]	+	[[Category: Lou, Z]]
-	[[Category: Qin, L.]]	+	[[Category: Qin, L]]
-	[[Category: Rao, Z.]]	+	[[Category: Rao, Z]]
-	[[Category: Tien, P.]]	+	[[Category: Tien, P]]
-	[[Category: Xu, Y.]]	+	[[Category: Xu, Y]]
	[[Category: Coronavirus]]		[[Category: Coronavirus]]
	[[Category: Fusion core]]		[[Category: Fusion core]]

OCA at 18:51, 29 September 2014

OCA — Mon, 29 Sep 2014 18:51:59 GMT

←Older revision		Revision as of 18:51, 29 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1wdf.~~png~~\|~~left~~\|200px]]	+	==crystal structure of MHV spike protein fusion core==
		+	<StructureSection load='1wdf' size='340' side='right' caption='[[1wdf]], [[Resolution\|resolution]] 2.50Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1wdf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_hepatitis_virus_strain_a59 Murine hepatitis virus strain a59]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDF FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wdg\|1wdg]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wdf RCSB], [http://www.ebi.ac.uk/pdbsum/1wdf PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/1wdf_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The surface transmembrane glycoprotein is responsible for mediating virion attachment to cell and subsequent virus-cell membrane fusion. However, the molecular mechanisms for the viral entry of coronaviruses remain poorly understood. The crystal structure of the fusion core of mouse hepatitis virus S protein, which represents the first fusion core structure of any coronavirus, reveals a central hydrophobic coiled coil trimer surrounded by three helices in an oblique, antiparallel manner. This structure shares significant similarity with both the low pH-induced conformation of influenza hemagglutinin and fusion core of HIV gp41, indicating that the structure represents a fusion-active state formed after several conformational changes. Our results also indicate that the mechanisms for the viral fusion of coronaviruses are similar to those of influenza virus and HIV. The coiled coil structure has unique features, which are different from other viral fusion cores. Highly conserved heptad repeat 1 (HR1) and HR2 regions in coronavirus spike proteins indicate a similar three-dimensional structure among these fusion cores and common mechanisms for the viral fusion. We have proposed the binding regions of HR1 and HR2 of other coronaviruses and a structure model of their fusion core based on our mouse hepatitis virus fusion core structure and sequence alignment. Drug discovery strategies aimed at inhibiting viral entry by blocking hairpin formation may be applied to the inhibition of a number of emerging infectious diseases, including severe acute respiratory syndrome.

-	~~{{STRUCTURE_1wdf\| PDB=1wdf \| SCENE= }}~~	+	Structural basis for coronavirus-mediated membrane fusion. Crystal structure of mouse hepatitis virus spike protein fusion core.,Xu Y, Liu Y, Lou Z, Qin L, Li X, Bai Z, Pang H, Tien P, Gao GF, Rao Z J Biol Chem. 2004 Jul 16;279(29):30514-22. Epub 2004 Apr 27. PMID:15123674<ref>PMID:15123674</ref>

-	~~===crystal structure~~ of ~~MHV spike protein fusion core===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15123674}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1wdf]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Murine_hepatitis_virus_strain_a59 Murine hepatitis virus strain a59]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015123674<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Murine hepatitis virus strain a59]]		[[Category: Murine hepatitis virus strain a59]]
	[[Category: Bai, Z.]]		[[Category: Bai, Z.]]

OCA: Protected "1wdf" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 11:39:23 GMT

Protected "1wdf" [edit=sysop:move=sysop]

←Older revision

Revision as of 11:39, 5 January 2013

OCA at 11:39, 5 January 2013

OCA — Sat, 05 Jan 2013 11:39:20 GMT

←Older revision		Revision as of 11:39, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1wdf.png\|left\|200px]]		[[Image:1wdf.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1wdf", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1wdf\| PDB=1wdf \| SCENE= }}		{{STRUCTURE_1wdf\| PDB=1wdf \| SCENE= }}

	===crystal structure of MHV spike protein fusion core===		===crystal structure of MHV spike protein fusion core===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15123674}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15123674 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15123674}}		{{ABSTRACT_PUBMED_15123674}}

	==About this Structure==		==About this Structure==
-	~~1WDF~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/~~Murine_hepatitis_virus~~ Murine hepatitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA].	+	[[1wdf]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Murine_hepatitis_virus_strain_a59 Murine hepatitis virus strain a59]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15123674~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015123674</ref><references group="xtra"/>
-	[[Category: Murine hepatitis virus]]	+	[[Category: Murine hepatitis virus strain a59]]
	[[Category: Bai, Z.]]		[[Category: Bai, Z.]]
	[[Category: Gao, G F.]]		[[Category: Gao, G F.]]
Line 34:		Line 22:
	[[Category: Tien, P.]]		[[Category: Tien, P.]]
	[[Category: Xu, Y.]]		[[Category: Xu, Y.]]
-	[[Category: Coiled coil]]
	[[Category: Coronavirus]]		[[Category: Coronavirus]]
-	[[Category: Crystal structure]]
	[[Category: Fusion core]]		[[Category: Fusion core]]
	[[Category: Heptad repeat]]		[[Category: Heptad repeat]]
	[[Category: Mhv]]		[[Category: Mhv]]
	[[Category: Viral entry]]		[[Category: Viral entry]]
-		+	[[Category: Viral protein]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 04:38:28 2009''~~	+

OCA at 02:38, 18 February 2009

OCA — Wed, 18 Feb 2009 02:38:28 GMT

←Older revision		Revision as of 02:38, 18 February 2009
Line 20:		Line 20:

	==About this Structure==		==About this Structure==
-	1WDF is a ~~[[Single protein]]~~ structure of ~~sequence~~ from [http://en.wikipedia.org/wiki/Murine_hepatitis_virus Murine hepatitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA].	+	1WDF is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Murine_hepatitis_virus Murine hepatitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDF OCA].

	==Reference==		==Reference==
-	Structural basis for coronavirus-mediated membrane fusion. Crystal structure of mouse hepatitis virus spike protein fusion core., Xu Y, Liu Y, Lou Z, Qin L, Li X, Bai Z, Pang H, Tien P, Gao GF, Rao Z, J Biol Chem. 2004 Jul 16;279(29):30514-22. Epub 2004 Apr 27. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15123674 15123674]~~	+	<ref group="xtra">PMID:15123674</ref><references group="xtra"/>
	[[Category: Murine hepatitis virus]]		[[Category: Murine hepatitis virus]]
-	[[Category: Single protein]]
	[[Category: Bai, Z.]]		[[Category: Bai, Z.]]
	[[Category: Gao, G F.]]		[[Category: Gao, G F.]]
Line 43:		Line 42:
	[[Category: Viral entry]]		[[Category: Viral entry]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Tue Jul 29 11~~:23:~~46 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Feb 18 04:38:28 2009''