1wdn - Revision history

OCA at 08:47, 14 February 2024

OCA — Wed, 14 Feb 2024 08:47:41 GMT

←Older revision		Revision as of 08:47, 14 February 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WDN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WDN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.94Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdn OCA], [https://pdbe.org/1wdn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wdn RCSB], [https://www.ebi.ac.uk/pdbsum/1wdn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wdn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdn OCA], [https://pdbe.org/1wdn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wdn RCSB], [https://www.ebi.ac.uk/pdbsum/1wdn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wdn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GLNH_ECOLI GLNH_ECOLI]] Involved in a glutamine-transport system GlnHPQ.	+	[https://www.uniprot.org/uniprot/GLNH_ECOLI GLNH_ECOLI] Involved in a glutamine-transport system GlnHPQ.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 19:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wdn ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wdn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The crystal structure of the glutamine-binding protein (GlnBP) complexed with its ligand (Gln) was determined and refined to 1.94 A resolution. This ellipsoidal protein has two globular domains and is approximately 52 Ax40 Ax35 A in size. The glutamine ligand is located in the cleft between the two domains and stablized by hydrogen bondings and ionic interactions with Asp10, Gly68, Thr70, Ala67, Asp157, Arg75, Lys115, Gly119 and His156. The aliphatic portion of the glutamine ligand is sandwiched in a hydrophobic pocket formed between Phe13 and Phe50 and has 21 van der Waals contacts with GlnBP. Lys115 and His156, that are unique to GlnBP among amino acid binding proteins, apparently contribute to the ligand binding specificity of GlnBP. Asp10 is within 3 A of Lys115. These two residues are over 10 A apart in the ligand-free form of the GlnBP. In addition, GlnBP-Gln exhibits a large-scale movement of the two hinges connecting the two globular domains upon ligand binding. The most significant changes are 41.1 degrees in the phi angle of Gly89 and 34.3 degrees in the psi angle of Glu181 from the first and the second hinge of the protein, respectively. Besides the original six hydrogen bonds, three extra hydrogen bonds can be observed between the two hinge strands upon ligand binding. A hydrogen bond network connects the large domain to the second hinge and a second hydrogen bond network coalesces the small domain to the same strand, both via interaction with the glutamine ligand. Although the two strands of the hinge connecting the domains do not directly participate in the ligand binding, Gln183 and Tyr185 from the second hinge may be involved in the cascade of the conformational change that is induced by ligand binding.
-
-	The structure of glutamine-binding protein complexed with glutamine at 1.94 A resolution: comparisons with other amino acid binding proteins.,Sun YJ, Rose J, Wang BC, Hsiao CD J Mol Biol. 1998 Apr 24;278(1):219-29. PMID:9571045<ref>PMID:9571045</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1wdn" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hsiao, C D]]	+	[[Category: Hsiao C-D]]
-	[[Category: Rose, J]]	+	[[Category: Rose J]]
-	[[Category: Sun, Y J]]	+	[[Category: Sun Y-J]]
-	[[Category: Wang, B C]]	+	[[Category: Wang B-C]]
-	~~[[Category: Binding protein]]~~	+
-	~~[[Category: Closed form]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Glnbp~~]]	+

OCA at 16:19, 27 October 2021

OCA — Wed, 27 Oct 2021 16:19:01 GMT

←Older revision		Revision as of 16:19, 27 October 2021
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	<StructureSection load='1wdn' size='340' side='right'caption='[[1wdn]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='1wdn' size='340' side='right'caption='[[1wdn]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1WDN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WDN FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdn OCA], [~~http~~://pdbe.org/1wdn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1wdn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1wdn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1wdn ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdn OCA], [https://pdbe.org/1wdn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wdn RCSB], [https://www.ebi.ac.uk/pdbsum/1wdn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wdn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GLNH_ECOLI GLNH_ECOLI]] Involved in a glutamine-transport system GlnHPQ.	+	[[https://www.uniprot.org/uniprot/GLNH_ECOLI GLNH_ECOLI]] Involved in a glutamine-transport system GlnHPQ.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 17:09, 22 January 2020

OCA — Wed, 22 Jan 2020 17:09:46 GMT

←Older revision		Revision as of 17:09, 22 January 2020
Line 1:		Line 1:

	==GLUTAMINE-BINDING PROTEIN==		==GLUTAMINE-BINDING PROTEIN==
-	<StructureSection load='1wdn' size='340' side='right' caption='[[1wdn]], [[Resolution\|resolution]] 1.94Å' scene=''>	+	<StructureSection load='1wdn' size='340' side='right'caption='[[1wdn]], [[Resolution\|resolution]] 1.94Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDN FirstGlance]. <br>
Line 33:		Line 33:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
		+	[[Category: Large Structures]]
	[[Category: Hsiao, C D]]		[[Category: Hsiao, C D]]
	[[Category: Rose, J]]		[[Category: Rose, J]]

OCA at 15:56, 11 April 2018

OCA — Wed, 11 Apr 2018 15:56:50 GMT

←Older revision		Revision as of 15:56, 11 April 2018
Line 1:		Line 1:
		+
	==GLUTAMINE-BINDING PROTEIN==		==GLUTAMINE-BINDING PROTEIN==
	<StructureSection load='1wdn' size='340' side='right' caption='[[1wdn]], [[Resolution\|resolution]] 1.94Å' scene=''>		<StructureSection load='1wdn' size='340' side='right' caption='[[1wdn]], [[Resolution\|resolution]] 1.94Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdn OCA], [http://pdbe.org/1wdn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wdn RCSB], [http://www.ebi.ac.uk/pdbsum/1wdn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdn OCA], [http://pdbe.org/1wdn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wdn RCSB], [http://www.ebi.ac.uk/pdbsum/1wdn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wdn ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 12:		Line 13:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/1wdn_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/1wdn_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wdn ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 10:23, 11 September 2015

OCA — Fri, 11 Sep 2015 10:23:16 GMT

←Older revision		Revision as of 10:23, 11 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDN FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wdn RCSB], [http://www.ebi.ac.uk/pdbsum/1wdn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdn OCA], [http://pdbe.org/1wdn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wdn RCSB], [http://www.ebi.ac.uk/pdbsum/1wdn PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 26:		Line 26:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1wdn" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 15:08, 25 December 2014

OCA — Thu, 25 Dec 2014 15:08:50 GMT

←Older revision		Revision as of 15:08, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDN FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDN FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wdn RCSB], [http://www.ebi.ac.uk/pdbsum/1wdn PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wdn RCSB], [http://www.ebi.ac.uk/pdbsum/1wdn PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GLNH_ECOLI GLNH_ECOLI]] Involved in a glutamine-transport system GlnHPQ.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 29:		Line 31:
	</StructureSection>		</StructureSection>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Hsiao, C D.]]	+	[[Category: Hsiao, C D]]
-	[[Category: Rose, J.]]	+	[[Category: Rose, J]]
-	[[Category: Sun, Y J.]]	+	[[Category: Sun, Y J]]
-	[[Category: Wang, B C.]]	+	[[Category: Wang, B C]]
	[[Category: Binding protein]]		[[Category: Binding protein]]
	[[Category: Closed form]]		[[Category: Closed form]]
	[[Category: Complex]]		[[Category: Complex]]
	[[Category: Glnbp]]		[[Category: Glnbp]]

OCA at 18:19, 29 September 2014

OCA — Mon, 29 Sep 2014 18:19:13 GMT

←Older revision		Revision as of 18:19, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1wdn.~~png\|left~~\|200px]]	+	==GLUTAMINE-BINDING PROTEIN==
		+	<StructureSection load='1wdn' size='340' side='right' caption='[[1wdn]], [[Resolution\|resolution]] 1.94Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1wdn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WDN FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLN:GLUTAMINE'>GLN</scene><br>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wdn OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wdn RCSB], [http://www.ebi.ac.uk/pdbsum/1wdn PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wd/1wdn_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The crystal structure of the glutamine-binding protein (GlnBP) complexed with its ligand (Gln) was determined and refined to 1.94 A resolution. This ellipsoidal protein has two globular domains and is approximately 52 Ax40 Ax35 A in size. The glutamine ligand is located in the cleft between the two domains and stablized by hydrogen bondings and ionic interactions with Asp10, Gly68, Thr70, Ala67, Asp157, Arg75, Lys115, Gly119 and His156. The aliphatic portion of the glutamine ligand is sandwiched in a hydrophobic pocket formed between Phe13 and Phe50 and has 21 van der Waals contacts with GlnBP. Lys115 and His156, that are unique to GlnBP among amino acid binding proteins, apparently contribute to the ligand binding specificity of GlnBP. Asp10 is within 3 A of Lys115. These two residues are over 10 A apart in the ligand-free form of the GlnBP. In addition, GlnBP-Gln exhibits a large-scale movement of the two hinges connecting the two globular domains upon ligand binding. The most significant changes are 41.1 degrees in the phi angle of Gly89 and 34.3 degrees in the psi angle of Glu181 from the first and the second hinge of the protein, respectively. Besides the original six hydrogen bonds, three extra hydrogen bonds can be observed between the two hinge strands upon ligand binding. A hydrogen bond network connects the large domain to the second hinge and a second hydrogen bond network coalesces the small domain to the same strand, both via interaction with the glutamine ligand. Although the two strands of the hinge connecting the domains do not directly participate in the ligand binding, Gln183 and Tyr185 from the second hinge may be involved in the cascade of the conformational change that is induced by ligand binding.

-	~~{{STRUCTURE_1wdn\| PDB=1wdn \| SCENE= }}~~	+	The structure of glutamine-binding protein complexed with glutamine at 1.94 A resolution: comparisons with other amino acid binding proteins.,Sun YJ, Rose J, Wang BC, Hsiao CD J Mol Biol. 1998 Apr 24;278(1):219-29. PMID:9571045<ref>PMID:9571045</ref>

-	~~===GLUTAMINE-BINDING PROTEIN===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_9571045}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1wdn]] is~~ a ~~1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Escherichia_coli Escherichia coli]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:009571045<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Hsiao, C D.]]		[[Category: Hsiao, C D.]]

OCA: Protected "1wdn" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 10:53:51 GMT

Protected "1wdn" [edit=sysop:move=sysop]

←Older revision

Revision as of 10:53, 5 January 2013

OCA at 10:53, 5 January 2013

OCA — Sat, 05 Jan 2013 10:53:46 GMT

←Older revision		Revision as of 10:53, 5 January 2013
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-	{{Seed}}
	[[Image:1wdn.png\|left\|200px]]		[[Image:1wdn.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1wdn", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1wdn\| PDB=1wdn \| SCENE= }}		{{STRUCTURE_1wdn\| PDB=1wdn \| SCENE= }}

	===GLUTAMINE-BINDING PROTEIN===		===GLUTAMINE-BINDING PROTEIN===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_9571045}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 9571045 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_9571045}}		{{ABSTRACT_PUBMED_9571045}}

	==About this Structure==		==About this Structure==
-	~~1WDN~~ is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA].	+	[[1wdn]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~9571045~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:009571045</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
	[[Category: Hsiao, C D.]]		[[Category: Hsiao, C D.]]
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	[[Category: Complex]]		[[Category: Complex]]
	[[Category: Glnbp]]		[[Category: Glnbp]]
-	[[Category: Peptide]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:49:00 2009''

OCA at 17:49, 17 February 2009

OCA — Tue, 17 Feb 2009 17:49:00 GMT

←Older revision		Revision as of 17:49, 17 February 2009
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	==About this Structure==		==About this Structure==
-	1WDN is a ~~[[Single protein]]~~ structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA].	+	1WDN is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WDN OCA].

	==Reference==		==Reference==
-	The structure of glutamine-binding protein complexed with glutamine at 1.94 A resolution: comparisons with other amino acid binding proteins., Sun YJ, Rose J, Wang BC, Hsiao CD, J Mol Biol. 1998 Apr 24;278(1):219-29. PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/9571045 9571045]~~	+	<ref group="xtra">PMID:9571045</ref><references group="xtra"/>
	[[Category: Escherichia coli]]		[[Category: Escherichia coli]]
-	[[Category: Single protein]]
	[[Category: Hsiao, C D.]]		[[Category: Hsiao, C D.]]
	[[Category: Rose, J.]]		[[Category: Rose, J.]]
Line 36:		Line 35:
	[[Category: Peptide]]		[[Category: Peptide]]

-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue ~~Jul 29 03~~:43:~~33 2008~~''	+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 19:49:00 2009''