1wnx - Revision history

OCA at 13:46, 9 May 2024

OCA — Thu, 09 May 2024 13:46:04 GMT

←Older revision		Revision as of 13:46, 9 May 2024
Line 3:		Line 3:
	<StructureSection load='1wnx' size='340' side='right'caption='[[1wnx]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='1wnx' size='340' side='right'caption='[[1wnx]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wnx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_diphtheriae"_kruse_in_flugge_1886 "bacillus~~ diphtheriae~~" kruse in flugge 1886~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WNX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wnx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WNX FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>~~, ~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.85Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max-height: 3em;~~'>~~[[1wnv\|1wnv]], [[1wnw\|1wnw]], [[1iw0\|1iw0]], [[1iw1\|1iw1]], [[1v8x\|1v8x]]~~</~~div~~><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Heme_oxygenase Heme oxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.3 1.14.99.3]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnx OCA], [https://pdbe.org/1wnx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wnx RCSB], [https://www.ebi.ac.uk/pdbsum/1wnx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wnx ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnx OCA], [https://pdbe.org/1wnx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wnx RCSB], [https://www.ebi.ac.uk/pdbsum/1wnx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wnx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/HMUO_CORDI HMUO_CORDI]] Allows the bacteria to use the host heme as an iron source. Involved in the oxidation of heme and subsequent release of iron from the heme moiety.	+	[https://www.uniprot.org/uniprot/HMUO_CORDI HMUO_CORDI] Allows the bacteria to use the host heme as an iron source. Involved in the oxidation of heme and subsequent release of iron from the heme moiety.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ diphtheriae ~~kruse in flugge 1886]]~~	+	[[Category: Corynebacterium diphtheriae]]
-	~~[[Category: Heme oxygenase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Ikeda-Saito, M]]	+	[[Category: Ikeda-Saito M]]
-	[[Category: Matsui, T]]	+	[[Category: Matsui T]]
-	[[Category: Unno, M]]	+	[[Category: Unno M]]
-	~~[[Category: Alpha-helix]]~~	+
-	~~[[Category: Heme]]~~	+
-	~~[[Category: Oxidoreductase~~]]	+

OCA at 16:23, 27 October 2021

OCA — Wed, 27 Oct 2021 16:23:01 GMT

←Older revision		Revision as of 16:23, 27 October 2021
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	<StructureSection load='1wnx' size='340' side='right'caption='[[1wnx]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='1wnx' size='340' side='right'caption='[[1wnx]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wnx]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"bacillus_diphtheriae"_kruse_in_flugge_1886 "bacillus diphtheriae" kruse in flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1WNX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wnx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_diphtheriae"_kruse_in_flugge_1886 "bacillus diphtheriae" kruse in flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WNX FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnv\|1wnv]], [[1wnw\|1wnw]], [[1iw0\|1iw0]], [[1iw1\|1iw1]], [[1v8x\|1v8x]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1wnv\|1wnv]], [[1wnw\|1wnw]], [[1iw0\|1iw0]], [[1iw1\|1iw1]], [[1v8x\|1v8x]]</div></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Heme_oxygenase Heme oxygenase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.3 1.14.99.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Heme_oxygenase Heme oxygenase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.3 1.14.99.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1wnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnx OCA], [~~http~~://pdbe.org/1wnx PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1wnx RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1wnx PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1wnx ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnx OCA], [https://pdbe.org/1wnx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wnx RCSB], [https://www.ebi.ac.uk/pdbsum/1wnx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wnx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/HMUO_CORDI HMUO_CORDI]] Allows the bacteria to use the host heme as an iron source. Involved in the oxidation of heme and subsequent release of iron from the heme moiety.	+	[[https://www.uniprot.org/uniprot/HMUO_CORDI HMUO_CORDI]] Allows the bacteria to use the host heme as an iron source. Involved in the oxidation of heme and subsequent release of iron from the heme moiety.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 18:02, 22 January 2020

OCA — Wed, 22 Jan 2020 18:02:42 GMT

←Older revision		Revision as of 18:02, 22 January 2020
Line 1:		Line 1:

	==D136E mutant of Heme Oxygenase from Corynebacterium diphtheriae (HmuO)==		==D136E mutant of Heme Oxygenase from Corynebacterium diphtheriae (HmuO)==
-	<StructureSection load='1wnx' size='340' side='right' caption='[[1wnx]], [[Resolution\|resolution]] 1.85Å' scene=''>	+	<StructureSection load='1wnx' size='340' side='right'caption='[[1wnx]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wnx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_diphtheriae"_kruse_in_flugge_1886 "bacillus diphtheriae" kruse in flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WNX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wnx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_diphtheriae"_kruse_in_flugge_1886 "bacillus diphtheriae" kruse in flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WNX FirstGlance]. <br>
Line 32:		Line 32:

	==See Also==		==See Also==
-	*[[Heme oxygenase\|Heme oxygenase]]	+	*[[Heme oxygenase 3D structures\|Heme oxygenase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 39:		Line 39:
	[[Category: Bacillus diphtheriae kruse in flugge 1886]]		[[Category: Bacillus diphtheriae kruse in flugge 1886]]
	[[Category: Heme oxygenase]]		[[Category: Heme oxygenase]]
		+	[[Category: Large Structures]]
	[[Category: Ikeda-Saito, M]]		[[Category: Ikeda-Saito, M]]
	[[Category: Matsui, T]]		[[Category: Matsui, T]]

OCA at 16:43, 11 April 2018

OCA — Wed, 11 Apr 2018 16:43:01 GMT

←Older revision		Revision as of 16:43, 11 April 2018
Line 1:		Line 1:
		+
	==D136E mutant of Heme Oxygenase from Corynebacterium diphtheriae (HmuO)==		==D136E mutant of Heme Oxygenase from Corynebacterium diphtheriae (HmuO)==
	<StructureSection load='1wnx' size='340' side='right' caption='[[1wnx]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='1wnx' size='340' side='right' caption='[[1wnx]], [[Resolution\|resolution]] 1.85Å' scene=''>
Line 6:		Line 7:
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnv\|1wnv]], [[1wnw\|1wnw]], [[1iw0\|1iw0]], [[1iw1\|1iw1]], [[1v8x\|1v8x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnv\|1wnv]], [[1wnw\|1wnw]], [[1iw0\|1iw0]], [[1iw1\|1iw1]], [[1v8x\|1v8x]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Heme_oxygenase Heme oxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.3 1.14.99.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Heme_oxygenase Heme oxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.3 1.14.99.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnx OCA], [http://pdbe.org/1wnx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wnx RCSB], [http://www.ebi.ac.uk/pdbsum/1wnx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnx OCA], [http://pdbe.org/1wnx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wnx RCSB], [http://www.ebi.ac.uk/pdbsum/1wnx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wnx ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wn/1wnx_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wn/1wnx_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 05:17, 9 February 2016

OCA — Tue, 09 Feb 2016 05:17:23 GMT

←Older revision		Revision as of 05:17, 9 February 2016
Line 18:		Line 18:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wnx ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 18:59, 10 September 2015

OCA — Thu, 10 Sep 2015 18:59:56 GMT

←Older revision		Revision as of 18:59, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1wnx' size='340' side='right' caption='[[1wnx]], [[Resolution\|resolution]] 1.85Å' scene=''>		<StructureSection load='1wnx' size='340' side='right' caption='[[1wnx]], [[Resolution\|resolution]] 1.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wnx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Corynebacterium_diphtheriae Corynebacterium~~ diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WNX FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wnx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_diphtheriae"_kruse_in_flugge_1886 "bacillus diphtheriae" kruse in flugge 1886]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WNX FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnv\|1wnv]], [[1wnw\|1wnw]], [[1iw0\|1iw0]], [[1iw1\|1iw1]], [[1v8x\|1v8x]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnv\|1wnv]], [[1wnw\|1wnw]], [[1iw0\|1iw0]], [[1iw1\|1iw1]], [[1v8x\|1v8x]]</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Heme_oxygenase Heme oxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.3 1.14.99.3] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Heme_oxygenase Heme oxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.3 1.14.99.3] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wnx RCSB], [http://www.ebi.ac.uk/pdbsum/1wnx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnx OCA], [http://pdbe.org/1wnx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wnx RCSB], [http://www.ebi.ac.uk/pdbsum/1wnx PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 28:		Line 28:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1wnx" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 35:		Line 36:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Corynebacterium~~ diphtheriae]]	+	[[Category: Bacillus diphtheriae kruse in flugge 1886]]
	[[Category: Heme oxygenase]]		[[Category: Heme oxygenase]]
	[[Category: Ikeda-Saito, M]]		[[Category: Ikeda-Saito, M]]

OCA at 04:41, 25 December 2014

OCA — Thu, 25 Dec 2014 04:41:45 GMT

←Older revision		Revision as of 04:41, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wnx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WNX FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wnx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WNX FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnv\|1wnv]], [[1wnw\|1wnw]], [[1iw0\|1iw0]], [[1iw1\|1iw1]], [[1v8x\|1v8x]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnv\|1wnv]], [[1wnw\|1wnw]], [[1iw0\|1iw0]], [[1iw1\|1iw1]], [[1v8x\|1v8x]]</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Heme_oxygenase Heme oxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.3 1.14.99.3] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Heme_oxygenase Heme oxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.3 1.14.99.3] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wnx RCSB], [http://www.ebi.ac.uk/pdbsum/1wnx PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wnx RCSB], [http://www.ebi.ac.uk/pdbsum/1wnx PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/HMUO_CORDI HMUO_CORDI]] Allows the bacteria to use the host heme as an iron source. Involved in the oxidation of heme and subsequent release of iron from the heme moiety.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 35:		Line 37:
	[[Category: Corynebacterium diphtheriae]]		[[Category: Corynebacterium diphtheriae]]
	[[Category: Heme oxygenase]]		[[Category: Heme oxygenase]]
-	[[Category: Ikeda-Saito, M.]]	+	[[Category: Ikeda-Saito, M]]
-	[[Category: Matsui, T.]]	+	[[Category: Matsui, T]]
-	[[Category: Unno, M.]]	+	[[Category: Unno, M]]
	[[Category: Alpha-helix]]		[[Category: Alpha-helix]]
	[[Category: Heme]]		[[Category: Heme]]
	[[Category: Oxidoreductase]]		[[Category: Oxidoreductase]]

OCA at 22:07, 28 September 2014

OCA — Sun, 28 Sep 2014 22:07:34 GMT

←Older revision		Revision as of 22:07, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1wnx.~~png~~\|~~left~~\|200px]]	+	==D136E mutant of Heme Oxygenase from Corynebacterium diphtheriae (HmuO)==
		+	<StructureSection load='1wnx' size='340' side='right' caption='[[1wnx]], [[Resolution\|resolution]] 1.85Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1wnx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WNX FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1wnv\|1wnv]], [[1wnw\|1wnw]], [[1iw0\|1iw0]], [[1iw1\|1iw1]], [[1v8x\|1v8x]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Heme_oxygenase Heme oxygenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.14.99.3 1.14.99.3] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wnx OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wnx RCSB], [http://www.ebi.ac.uk/pdbsum/1wnx PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/wn/1wnx_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Heme oxygenases found in mammals, plants, and bacteria catalyze degradation of heme using the same mechanism. Roles of distal Asp (Asp-136) residue in HmuO, a heme oxygenase of Corynebacterium diphtheriae, have been investigated by site-directed mutagenesis, enzyme kinetics, resonance Raman spectroscopy, and x-ray crystallography. Replacements of the Asp-136 by Ala and Phe resulted in reduced heme degradation activity due to the formation of ferryl heme, showing that the distal Asp is critical in HmuO heme oxygenase activity. D136N HmuO catalyzed heme degradation at a similar efficiency to wild type and D136E HmuO, implying that the carboxylate moiety is not required for the heme catabolism by HmuO. Resonance Raman results suggest that the inactive ferryl heme formation in the HmuO mutants is induced by disruption of the interaction between a reactive Fe-OOH species and an adjacent distal pocket water molecule. Crystal structural analysis of the HmuO mutants confirms partial disappearance of this nearby water in D136A HmuO. Our results provide the first experimental evidence for the catalytic importance of the nearby water molecule that can be universally critical in heme oxygenase catalysis and propose that the distal Asp helps in positioning the key water molecule at a position suitable for efficient activation of the Fe-OOH species.

-	~~{{STRUCTURE_1wnx\| PDB=1wnx \| SCENE= }}~~	+	Roles of distal Asp in heme oxygenase from Corynebacterium diphtheriae, HmuO: A water-driven oxygen activation mechanism.,Matsui T, Furukawa M, Unno M, Tomita T, Ikeda-Saito M J Biol Chem. 2005 Jan 28;280(4):2981-9. Epub 2004 Nov 4. PMID:15528205<ref>PMID:15528205</ref>

-	~~===D136E mutant~~ of ~~Heme Oxygenase from Corynebacterium diphtheriae (HmuO)===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15528205}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1wnx]] is a 2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA].~~	+

	==See Also==		==See Also==
	*[[Heme oxygenase\|Heme oxygenase]]		*[[Heme oxygenase\|Heme oxygenase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015528205<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Corynebacterium diphtheriae]]		[[Category: Corynebacterium diphtheriae]]
	[[Category: Heme oxygenase]]		[[Category: Heme oxygenase]]

OCA at 12:46, 20 October 2012

OCA — Sat, 20 Oct 2012 12:46:06 GMT

←Older revision		Revision as of 12:46, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1wnx]] is a 2 chain structure ~~of [[Heme oxygenase]]~~ with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA].	+	[[1wnx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Corynebacterium_diphtheriae Corynebacterium diphtheriae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WNX OCA].

	==See Also==		==See Also==

OCA: Protected "1wnx" [edit=sysop:move=sysop]

OCA — Thu, 26 Jul 2012 09:49:26 GMT

Protected "1wnx" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:49, 26 July 2012