1wsa - Revision history

OCA at 08:48, 14 February 2024

OCA — Wed, 14 Feb 2024 08:48:00 GMT

←Older revision		Revision as of 08:48, 14 February 2024
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	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WSA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WSA FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>[~~https://en.wikipedia.org/wiki/Asparaginase Asparaginase~~]~~, with EC number [https://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsa OCA], [https://pdbe.org/1wsa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wsa RCSB], [https://www.ebi.ac.uk/pdbsum/1wsa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wsa ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsa OCA], [https://pdbe.org/1wsa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wsa RCSB], [https://www.ebi.ac.uk/pdbsum/1wsa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wsa ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/ASPG_WOLSU ASPG_WOLSU]
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 17:		Line 19:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wsa ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wsa ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The amino acid sequence and tertiary structure of Wolinella succinogenes L-asparaginase were determined, and were compared with the structures of other type-II bacterial L-asparaginases. Each chain of this homotetrameric enzyme consists of 330 residues. The amino acid sequence is 40-50% identical to the sequences of related proteins from other bacterial sources, and all residues previously shown to be crucial for the catalytic action of these enzymes are identical. Differences between the amino acid sequence of W. succinogenes L-asparaginase and that of related enzymes are discussed in terms of the possible influence on the substrate specificity. The overall fold of the protein subunit is almost identical to that observed for other L-asparaginases. Two fragments in each subunit, a very highly flexible loop (approximately 20 amino acids) that forms part of the active site, and the N-terminus (two amino acids), are not defined in the structure. The orientation of Thr14, a residue probably involved in the catalytic activity, indicates the absence of ligand in the active-site pocket. The rigid part of the active site, which includes the asparaginase triad Thr93-Lys 166-Asp94, is structurally very highly conserved with equivalent regions found in other type-II bacterial L-asparaginases.
-
-	Crystal structure and amino acid sequence of Wolinella succinogenes L-asparaginase.,Lubkowski J, Palm GJ, Gilliland GL, Derst C, Rohm KH, Wlodawer A Eur J Biochem. 1996 Oct 1;241(1):201-7. PMID:8898907<ref>PMID:8898907</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1wsa" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Asparaginase 3D structures\|Asparaginase 3D structures]]		*[[Asparaginase 3D structures\|Asparaginase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Asparaginase]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Wolinella succinogenes]]		[[Category: Wolinella succinogenes]]
-	[[Category: Derst, C]]	+	[[Category: Derst C]]
-	[[Category: Gilliland~~, G L~~]]	+	[[Category: Gilliland GL]]
-	[[Category: Lubkowski, J]]	+	[[Category: Lubkowski J]]
-	[[Category: Palm~~, G J~~]]	+	[[Category: Palm GJ]]
-	[[Category: Rohm, K H]]	+	[[Category: Rohm K-H]]
-	[[Category: Wlodawer, A]]	+	[[Category: Wlodawer A]]
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: Periplasmic~~]]	+

OCA at 16:25, 27 October 2021

OCA — Wed, 27 Oct 2021 16:25:59 GMT

←Older revision		Revision as of 16:25, 27 October 2021
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	<StructureSection load='1wsa' size='340' side='right'caption='[[1wsa]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1wsa' size='340' side='right'caption='[[1wsa]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1WSA FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WSA FirstGlance]. <br>
-	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [~~http~~://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1wsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsa OCA], [~~http~~://pdbe.org/1wsa PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1wsa RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1wsa PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1wsa ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsa OCA], [https://pdbe.org/1wsa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wsa RCSB], [https://www.ebi.ac.uk/pdbsum/1wsa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wsa ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==

OCA at 17:46, 22 January 2020

OCA — Wed, 22 Jan 2020 17:46:26 GMT

←Older revision		Revision as of 17:46, 22 January 2020
Line 1:		Line 1:

	==STRUCTURE OF L-ASPARAGINASE II PRECURSOR==		==STRUCTURE OF L-ASPARAGINASE II PRECURSOR==
-	<StructureSection load='1wsa' size='340' side='right' caption='[[1wsa]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1wsa' size='340' side='right'caption='[[1wsa]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WSA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WSA FirstGlance]. <br>
Line 28:		Line 28:

	==See Also==		==See Also==
-	*[[Asparaginase\|Asparaginase]]	+	*[[Asparaginase 3D structures\|Asparaginase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 34:		Line 34:
	</StructureSection>		</StructureSection>
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
		+	[[Category: Large Structures]]
	[[Category: Wolinella succinogenes]]		[[Category: Wolinella succinogenes]]
	[[Category: Derst, C]]		[[Category: Derst, C]]

OCA at 16:29, 11 April 2018

OCA — Wed, 11 Apr 2018 16:29:39 GMT

←Older revision		Revision as of 16:29, 11 April 2018
Line 1:		Line 1:
		+
	==STRUCTURE OF L-ASPARAGINASE II PRECURSOR==		==STRUCTURE OF L-ASPARAGINASE II PRECURSOR==
	<StructureSection load='1wsa' size='340' side='right' caption='[[1wsa]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1wsa' size='340' side='right' caption='[[1wsa]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WSA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WSA FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsa OCA], [http://pdbe.org/1wsa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wsa RCSB], [http://www.ebi.ac.uk/pdbsum/1wsa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsa OCA], [http://pdbe.org/1wsa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wsa RCSB], [http://www.ebi.ac.uk/pdbsum/1wsa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wsa ProSAT]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 10:		Line 11:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/1wsa_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/1wsa_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 03:11, 9 February 2016

OCA — Tue, 09 Feb 2016 03:11:00 GMT

←Older revision		Revision as of 03:11, 9 February 2016
Line 14:		Line 14:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1wsa ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 18:02, 10 September 2015

OCA — Thu, 10 Sep 2015 18:02:04 GMT

←Older revision		Revision as of 18:02, 10 September 2015
Line 4:		Line 4:
	<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WSA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WSA FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wsa RCSB], [http://www.ebi.ac.uk/pdbsum/1wsa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsa OCA], [http://pdbe.org/1wsa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wsa RCSB], [http://www.ebi.ac.uk/pdbsum/1wsa PDBsum]</span></td></tr>
	</table>		</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
Line 24:		Line 24:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1wsa" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==

OCA at 12:56, 6 January 2015

OCA — Tue, 06 Jan 2015 12:56:02 GMT

←Older revision		Revision as of 12:56, 6 January 2015
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WSA FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WSA FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wsa RCSB], [http://www.ebi.ac.uk/pdbsum/1wsa PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wsa RCSB], [http://www.ebi.ac.uk/pdbsum/1wsa PDBsum]</span></td></tr>
-	<table>	+	</table>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 33:		Line 33:
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
	[[Category: Wolinella succinogenes]]		[[Category: Wolinella succinogenes]]
-	[[Category: Derst, C.]]	+	[[Category: Derst, C]]
-	[[Category: Gilliland, G L.]]	+	[[Category: Gilliland, G L]]
-	[[Category: Lubkowski, J.]]	+	[[Category: Lubkowski, J]]
-	[[Category: Palm, G J.]]	+	[[Category: Palm, G J]]
-	[[Category: Rohm, K H.]]	+	[[Category: Rohm, K H]]
-	[[Category: Wlodawer, A.]]	+	[[Category: Wlodawer, A]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Periplasmic]]		[[Category: Periplasmic]]

OCA at 19:38, 29 September 2014

OCA — Mon, 29 Sep 2014 19:38:51 GMT

←Older revision		Revision as of 19:38, 29 September 2014
Line 1:		Line 1:
-	[[~~Image~~:1wsa.~~png\|left~~\|200px]]	+	==STRUCTURE OF L-ASPARAGINASE II PRECURSOR==
		+	<StructureSection load='1wsa' size='340' side='right' caption='[[1wsa]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1wsa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WSA FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Asparaginase Asparaginase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.1.1 3.5.1.1] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wsa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wsa OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wsa RCSB], [http://www.ebi.ac.uk/pdbsum/1wsa PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ws/1wsa_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The amino acid sequence and tertiary structure of Wolinella succinogenes L-asparaginase were determined, and were compared with the structures of other type-II bacterial L-asparaginases. Each chain of this homotetrameric enzyme consists of 330 residues. The amino acid sequence is 40-50% identical to the sequences of related proteins from other bacterial sources, and all residues previously shown to be crucial for the catalytic action of these enzymes are identical. Differences between the amino acid sequence of W. succinogenes L-asparaginase and that of related enzymes are discussed in terms of the possible influence on the substrate specificity. The overall fold of the protein subunit is almost identical to that observed for other L-asparaginases. Two fragments in each subunit, a very highly flexible loop (approximately 20 amino acids) that forms part of the active site, and the N-terminus (two amino acids), are not defined in the structure. The orientation of Thr14, a residue probably involved in the catalytic activity, indicates the absence of ligand in the active-site pocket. The rigid part of the active site, which includes the asparaginase triad Thr93-Lys 166-Asp94, is structurally very highly conserved with equivalent regions found in other type-II bacterial L-asparaginases.

-	~~{{STRUCTURE_1wsa\| PDB=1wsa \| SCENE= }}~~	+	Crystal structure and amino acid sequence of Wolinella succinogenes L-asparaginase.,Lubkowski J, Palm GJ, Gilliland GL, Derst C, Rohm KH, Wlodawer A Eur J Biochem. 1996 Oct 1;241(1):201-7. PMID:8898907<ref>PMID:8898907</ref>

-	~~===STRUCTURE OF L-ASPARAGINASE II PRECURSOR===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_8898907}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1wsa]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Wolinella_succinogenes Wolinella succinogenes]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA].~~	+

	==See Also==		==See Also==
	*[[Asparaginase\|Asparaginase]]		*[[Asparaginase\|Asparaginase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:008898907<~~/~~ref~~><~~references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
	[[Category: Wolinella succinogenes]]		[[Category: Wolinella succinogenes]]

OCA: Protected "1wsa" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 12:52:29 GMT

Protected "1wsa" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:52, 5 January 2013

OCA at 12:52, 5 January 2013

OCA — Sat, 05 Jan 2013 12:52:26 GMT

←Older revision		Revision as of 12:52, 5 January 2013
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-	{{Seed}}
	[[Image:1wsa.png\|left\|200px]]		[[Image:1wsa.png\|left\|200px]]

-	<!--
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	{{STRUCTURE_1wsa\| PDB=1wsa \| SCENE= }}		{{STRUCTURE_1wsa\| PDB=1wsa \| SCENE= }}

	===STRUCTURE OF L-ASPARAGINASE II PRECURSOR===		===STRUCTURE OF L-ASPARAGINASE II PRECURSOR===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_8898907}}, adds the Publication Abstract to the page
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	{{ABSTRACT_PUBMED_8898907}}		{{ABSTRACT_PUBMED_8898907}}

	==About this Structure==		==About this Structure==
-	~~1WSA~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA].	+	[[1wsa]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Wolinella_succinogenes Wolinella succinogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WSA OCA].
		+
		+	==See Also==
		+	*[[Asparaginase\|Asparaginase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~8898907~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:008898907</ref><references group="xtra"/>
	[[Category: Asparaginase]]		[[Category: Asparaginase]]
	[[Category: Wolinella succinogenes]]		[[Category: Wolinella succinogenes]]
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	[[Category: Hydrolase]]		[[Category: Hydrolase]]
	[[Category: Periplasmic]]		[[Category: Periplasmic]]
-	[[Category: Signal]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Feb 17 05:59:42 2009''