1wtw - Revision history

OCA at 10:30, 16 August 2023

OCA — Wed, 16 Aug 2023 10:30:02 GMT

←Older revision		Revision as of 10:30, 16 August 2023
Line 3:		Line 3:
	<StructureSection load='1wtw' size='340' side='right'caption='[[1wtw]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1wtw' size='340' side='right'caption='[[1wtw]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wtw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Atcc_33909 Atcc 33909~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WTW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wtw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WTW FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~<div style='overflow: auto; max~~-~~height: 3em;'>[[1azp\|1azp]]~~, [[~~1wto~~\|~~1wto~~]]~~, [[1wtp\|1wtp]], [[1wtq\|1wtq]], [[1wtr\|1wtr]], [[1wtx\|1wtx]]</div>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.2Å</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wtw OCA], [https://pdbe.org/1wtw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wtw RCSB], [https://www.ebi.ac.uk/pdbsum/1wtw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wtw ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wtw OCA], [https://pdbe.org/1wtw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wtw RCSB], [https://www.ebi.ac.uk/pdbsum/1wtw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wtw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~DN71_SULAC DN71_SULAC~~]~~] Constrain~~ negative DNA supercoils~~; may~~ be involved in maintaining the integrity of ~~their~~ genome at high temperature.	+	[https://www.uniprot.org/uniprot/DN7D_SULAC DN7D_SULAC] Can constrain negative DNA supercoils. May be involved in maintaining the integrity of the genome at high temperature.[UniProtKB:P61990]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 22:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 33909]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chen, C J]]	+	[[Category: Sulfolobus acidocaldarius]]
-	[[Category: Chen, C Y]]	+	[[Category: Chen C-J]]
-	[[Category: Chou, C C]]	+	[[Category: Chen C-Y]]
-	[[Category: Ko, T P]]	+	[[Category: Chou C-C]]
-	[[Category: Lin, T W]]	+	[[Category: Ko T-P]]
-	[[Category: Wang~~, A H.~~J]]	+	[[Category: Lin T-W]]
-	~~[[Category: Archea]]~~	+	[[Category: Wang AH-J]]
-	~~[[Category: Dna binding protein-dna complex]]~~	+
-	~~[[Category: Intercalation]]~~	+
-	~~[[Category: Kinked-dna]]~~	+
-	~~[[Category: Minor-groove dna binding]]~~	+
-	~~[[Category: Sac7d mutant~~]]	+

OCA at 16:27, 27 October 2021

OCA — Wed, 27 Oct 2021 16:27:18 GMT

←Older revision		Revision as of 16:27, 27 October 2021
Line 3:		Line 3:
	<StructureSection load='1wtw' size='340' side='right'caption='[[1wtw]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1wtw' size='340' side='right'caption='[[1wtw]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wtw]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Atcc_33909 Atcc 33909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1WTW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wtw]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_33909 Atcc 33909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1WTW FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1azp\|1azp]], [[1wto\|1wto]], [[1wtp\|1wtp]], [[1wtq\|1wtq]], [[1wtr\|1wtr]], [[1wtx\|1wtx]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1azp\|1azp]], [[1wto\|1wto]], [[1wtp\|1wtp]], [[1wtq\|1wtq]], [[1wtr\|1wtr]], [[1wtx\|1wtx]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wtw OCA], [~~http~~://pdbe.org/1wtw PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1wtw RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1wtw PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1wtw ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wtw OCA], [https://pdbe.org/1wtw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1wtw RCSB], [https://www.ebi.ac.uk/pdbsum/1wtw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1wtw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/DN71_SULAC DN71_SULAC]] Constrain negative DNA supercoils; may be involved in maintaining the integrity of their genome at high temperature.	+	[[https://www.uniprot.org/uniprot/DN71_SULAC DN71_SULAC]] Constrain negative DNA supercoils; may be involved in maintaining the integrity of their genome at high temperature.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 17:59, 22 January 2020

OCA — Wed, 22 Jan 2020 17:59:35 GMT

←Older revision		Revision as of 17:59, 22 January 2020
Line 1:		Line 1:

	==Hyperthermophile chromosomal protein SAC7D single mutant V26A in complex with DNA GCGATCGC==		==Hyperthermophile chromosomal protein SAC7D single mutant V26A in complex with DNA GCGATCGC==
-	<StructureSection load='1wtw' size='340' side='right' caption='[[1wtw]], [[Resolution\|resolution]] 2.20Å' scene=''>	+	<StructureSection load='1wtw' size='340' side='right'caption='[[1wtw]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1wtw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33909 Atcc 33909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WTW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wtw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33909 Atcc 33909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WTW FirstGlance]. <br>
Line 23:		Line 23:
	</StructureSection>		</StructureSection>
	[[Category: Atcc 33909]]		[[Category: Atcc 33909]]
		+	[[Category: Large Structures]]
	[[Category: Chen, C J]]		[[Category: Chen, C J]]
	[[Category: Chen, C Y]]		[[Category: Chen, C Y]]

OCA at 16:40, 11 April 2018

OCA — Wed, 11 Apr 2018 16:40:17 GMT

←Older revision		Revision as of 16:40, 11 April 2018
Line 1:		Line 1:
		+
	==Hyperthermophile chromosomal protein SAC7D single mutant V26A in complex with DNA GCGATCGC==		==Hyperthermophile chromosomal protein SAC7D single mutant V26A in complex with DNA GCGATCGC==
	<StructureSection load='1wtw' size='340' side='right' caption='[[1wtw]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1wtw' size='340' side='right' caption='[[1wtw]], [[Resolution\|resolution]] 2.20Å' scene=''>
Line 4:		Line 5:
	<table><tr><td colspan='2'>[[1wtw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33909 Atcc 33909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WTW FirstGlance]. <br>		<table><tr><td colspan='2'>[[1wtw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33909 Atcc 33909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WTW FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1azp\|1azp]], [[1wto\|1wto]], [[1wtp\|1wtp]], [[1wtq\|1wtq]], [[1wtr\|1wtr]], [[1wtx\|1wtx]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1azp\|1azp]], [[1wto\|1wto]], [[1wtp\|1wtp]], [[1wtq\|1wtq]], [[1wtr\|1wtr]], [[1wtx\|1wtx]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wtw OCA], [http://pdbe.org/1wtw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wtw RCSB], [http://www.ebi.ac.uk/pdbsum/1wtw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wtw OCA], [http://pdbe.org/1wtw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wtw RCSB], [http://www.ebi.ac.uk/pdbsum/1wtw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1wtw ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 08:08, 10 September 2015

OCA — Thu, 10 Sep 2015 08:08:59 GMT

←Older revision		Revision as of 08:08, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1wtw' size='340' side='right' caption='[[1wtw]], [[Resolution\|resolution]] 2.20Å' scene=''>		<StructureSection load='1wtw' size='340' side='right' caption='[[1wtw]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1wtw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Sulfolobus_acidocaldarius Sulfolobus acidocaldarius~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WTW FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1wtw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33909 Atcc 33909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WTW FirstGlance]. <br>
	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1azp\|1azp]], [[1wto\|1wto]], [[1wtp\|1wtp]], [[1wtq\|1wtq]], [[1wtr\|1wtr]], [[1wtx\|1wtx]]</td></tr>		</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1azp\|1azp]], [[1wto\|1wto]], [[1wtp\|1wtp]], [[1wtq\|1wtq]], [[1wtr\|1wtr]], [[1wtx\|1wtx]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wtw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wtw RCSB], [http://www.ebi.ac.uk/pdbsum/1wtw PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wtw OCA], [http://pdbe.org/1wtw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1wtw RCSB], [http://www.ebi.ac.uk/pdbsum/1wtw PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 16:		Line 16:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1wtw" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Sulfolobus acidocaldarius~~]]	+	[[Category: Atcc 33909]]
	[[Category: Chen, C J]]		[[Category: Chen, C J]]
	[[Category: Chen, C Y]]		[[Category: Chen, C Y]]

OCA at 20:50, 24 December 2014

OCA — Wed, 24 Dec 2014 20:50:30 GMT

←Older revision		Revision as of 20:50, 24 December 2014
Line 6:		Line 6:
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wtw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wtw RCSB], [http://www.ebi.ac.uk/pdbsum/1wtw PDBsum]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wtw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wtw RCSB], [http://www.ebi.ac.uk/pdbsum/1wtw PDBsum]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/DN71_SULAC DN71_SULAC]] Constrain negative DNA supercoils; may be involved in maintaining the integrity of their genome at high temperature.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 19:		Line 21:
	</StructureSection>		</StructureSection>
	[[Category: Sulfolobus acidocaldarius]]		[[Category: Sulfolobus acidocaldarius]]
-	[[Category: Chen, C J.]]	+	[[Category: Chen, C J]]
-	[[Category: Chen, C Y.]]	+	[[Category: Chen, C Y]]
-	[[Category: Chou, C C.]]	+	[[Category: Chou, C C]]
-	[[Category: Ko, T P.]]	+	[[Category: Ko, T P]]
-	[[Category: Lin, T W.]]	+	[[Category: Lin, T W]]
-	[[Category: Wang, A H.J.]]	+	[[Category: Wang, A H.J]]
	[[Category: Archea]]		[[Category: Archea]]
	[[Category: Dna binding protein-dna complex]]		[[Category: Dna binding protein-dna complex]]

OCA at 08:30, 8 October 2014

OCA — Wed, 08 Oct 2014 08:30:46 GMT

←Older revision		Revision as of 08:30, 8 October 2014
Line 1:		Line 1:
-	[[~~Image:~~1wtw.~~png~~\|~~left~~\|~~200px~~]]	+	==Hyperthermophile chromosomal protein SAC7D single mutant V26A in complex with DNA GCGATCGC==
		+	<StructureSection load='1wtw' size='340' side='right' caption='[[1wtw]], [[Resolution\|resolution]] 2.20Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1wtw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WTW FirstGlance]. <br>
		+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1azp\|1azp]], [[1wto\|1wto]], [[1wtp\|1wtp]], [[1wtq\|1wtq]], [[1wtr\|1wtr]], [[1wtx\|1wtx]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1wtw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1wtw OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1wtw RCSB], [http://www.ebi.ac.uk/pdbsum/1wtw PDBsum]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Sac7d, a small, abundant, sequence-general DNA-binding protein from the hyperthermophilic archaeon Sulfolobus acidocaldarius, causes a single-step sharp kink in DNA (approximately 60 degrees) via the intercalation of both Val26 and Met29. These two amino acids were systematically changed in size to probe their effects on DNA kinking. Eight crystal structures of five Sac7d mutant-DNA complexes have been analyzed. The DNA-binding pattern of the V26A and M29A single mutants is similar to that of the wild-type, whereas the V26A/M29A protein binds DNA without side chain intercalation, resulting in a smaller overall bending (approximately 50 degrees). The M29F mutant inserts the Phe29 side chain orthogonally to the C2pG3 step without stacking with base pairs, inducing a sharp kink (approximately 80 degrees). In the V26F/M29F-GCGATCGC complex, Phe26 intercalates deeply into DNA bases by stacking with the G3 base, whereas Phe29 is stacked on the G15 deoxyribose, in a way similar to those used by the TATA box-binding proteins. All mutants have reduced DNA-stabilizing ability, as indicated by their lower T m values. The DNA kink patterns caused by different combinations of hydrophobic side chains may be relevant in understanding the manner by which other minor groove-binding proteins interact with DNA.

-	~~{{STRUCTURE_1wtw\| PDB=1wtw \| SCENE= }}~~	+	Probing the DNA kink structure induced by the hyperthermophilic chromosomal protein Sac7d.,Chen CY, Ko TP, Lin TW, Chou CC, Chen CJ, Wang AH Nucleic Acids Res. 2005 Jan 14;33(1):430-8. Print 2005. PMID:15653643<ref>PMID:15653643</ref>

-	~~===Hyperthermophile chromosomal protein SAC7D single mutant V26A in complex with DNA GCGATCGC===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15653643}}~~	+	== References ==
-		+	<references/>
-	~~==About this Structure==~~	+	__TOC__
-	~~[[1wtw]] is~~ a ~~3 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA].~~	+	</StructureSection>
-		+
-	==~~Reference~~==	+
-	<~~ref group="xtra">PMID:015653643<~~/~~ref~~><~~references group="xtra"~~/>	+
	[[Category: Sulfolobus acidocaldarius]]		[[Category: Sulfolobus acidocaldarius]]
	[[Category: Chen, C J.]]		[[Category: Chen, C J.]]

OCA: Protected "1wtw" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 12:12:49 GMT

Protected "1wtw" [edit=sysop:move=sysop]

←Older revision

Revision as of 12:12, 5 January 2013

OCA at 12:12, 5 January 2013

OCA — Sat, 05 Jan 2013 12:12:46 GMT

←Older revision		Revision as of 12:12, 5 January 2013
Line 1:		Line 1:
-	{{Seed}}
	[[Image:1wtw.png\|left\|200px]]		[[Image:1wtw.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1wtw", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1wtw\| PDB=1wtw \| SCENE= }}		{{STRUCTURE_1wtw\| PDB=1wtw \| SCENE= }}

	===Hyperthermophile chromosomal protein SAC7D single mutant V26A in complex with DNA GCGATCGC===		===Hyperthermophile chromosomal protein SAC7D single mutant V26A in complex with DNA GCGATCGC===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15653643}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15653643 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15653643}}		{{ABSTRACT_PUBMED_15653643}}

	==About this Structure==		==About this Structure==
-	~~1WTW is a~~ [[~~Single protein~~]] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA].	+	[[1wtw]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Sulfolobus_acidocaldarius Sulfolobus acidocaldarius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WTW OCA].

	==Reference==		==Reference==
-	~~Probing the DNA kink structure induced by the hyperthermophilic chromosomal protein Sac7d., Chen CY, Ko TP, Lin TW, Chou CC, Chen CJ, Wang AH, Nucleic Acids Res. 2005 Jan 14;33(1):430-8. Print 2005.~~ PMID:~~[http:~~//~~www.ncbi.nlm.nih.gov/pubmed/15653643 15653643]~~	+	<ref group="xtra">PMID:015653643</ref><references group="xtra"/>
-	~~[[Category: Single protein]]~~	+
	[[Category: Sulfolobus acidocaldarius]]		[[Category: Sulfolobus acidocaldarius]]
	[[Category: Chen, C J.]]		[[Category: Chen, C J.]]
Line 33:		Line 20:
	[[Category: Wang, A H.J.]]		[[Category: Wang, A H.J.]]
	[[Category: Archea]]		[[Category: Archea]]
		+	[[Category: Dna binding protein-dna complex]]
	[[Category: Intercalation]]		[[Category: Intercalation]]
	[[Category: Kinked-dna]]		[[Category: Kinked-dna]]
	[[Category: Minor-groove dna binding]]		[[Category: Minor-groove dna binding]]
	[[Category: Sac7d mutant]]		[[Category: Sac7d mutant]]
-
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:20:37 2008''

OCA at 20:20, 27 July 2008

OCA — Sun, 27 Jul 2008 20:20:45 GMT

←Older revision		Revision as of 20:20, 27 July 2008
Line 1:		Line 1:
-	[[Image:1wtw.~~gif~~\|left\|200px]]	+	{{Seed}}
		+	[[Image:1wtw.png\|left\|200px]]

	<!--		<!--
Line 9:		Line 10:
	{{STRUCTURE_1wtw\| PDB=1wtw \| SCENE= }}		{{STRUCTURE_1wtw\| PDB=1wtw \| SCENE= }}

-	~~'''~~Hyperthermophile chromosomal protein SAC7D single mutant V26A in complex with DNA GCGATCGC~~'''~~	+	===Hyperthermophile chromosomal protein SAC7D single mutant V26A in complex with DNA GCGATCGC===


-	~~==Overview==~~	+	<!--
-	~~Sac7d, a small, abundant, sequence~~-~~general DNA~~-binding protein from the hyperthermophilic archaeon Sulfolobus acidocaldarius, causes a single-step sharp kink in DNA (approximately 60 degrees) via the intercalation of both Val26 and Met29. These two amino acids were systematically changed in size to probe their effects on DNA kinking. Eight crystal structures of five Sac7d mutant-DNA complexes have been analyzed. The ~~DNA-binding pattern of the V26A and M29A single mutants is similar to that of the wild-type~~, ~~whereas the V26A/M29A protein binds DNA without side chain intercalation~~, ~~resulting in a smaller overall bending (approximately 50 degrees). The M29F mutant inserts~~ the ~~Phe29 side chain orthogonally~~ to the ~~C2pG3 step without stacking with base pairs, inducing a sharp kink~~ (~~approximately 80 degrees~~)~~. In the V26F/M29F-GCGATCGC complex, Phe26 intercalates deeply into DNA bases by stacking with the G3 base~~, ~~whereas Phe29~~ is ~~stacked on~~ the ~~G15 deoxyribose, in a way similar to those used by the TATA box-binding proteins~~. ~~All mutants have reduced DNA~~-stabilizing ability, as indicated by their lower T m values. The DNA kink patterns caused by different combinations of hydrophobic side chains may be relevant in understanding the manner by which other minor groove-~~binding proteins interact with DNA.~~	+	The line below this paragraph, {{ABSTRACT_PUBMED_15653643}}, adds the Publication Abstract to the page
		+	(as it appears on PubMed at http://www.pubmed.gov), where 15653643 is the PubMed ID number.
		+	-->
		+	{{ABSTRACT_PUBMED_15653643}}

	==About this Structure==		==About this Structure==
Line 33:		Line 37:
	[[Category: Minor-groove dna binding]]		[[Category: Minor-groove dna binding]]
	[[Category: Sac7d mutant]]		[[Category: Sac7d mutant]]
-	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on ~~Sat May 3 14~~:07:50 2008''	+
		+	''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 23:20:37 2008''