1x7p - Revision history

OCA at 13:36, 13 March 2024

OCA — Wed, 13 Mar 2024 13:36:19 GMT

←Older revision		Revision as of 13:36, 13 March 2024
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	<StructureSection load='1x7p' size='340' side='right'caption='[[1x7p]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1x7p' size='340' side='right'caption='[[1x7p]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1x7p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"actinomyces_viridochromogenes"_krainsky_1914 "actinomyces~~ viridochromogenes~~" krainsky 1914~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X7P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1x7p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_viridochromogenes Streptomyces viridochromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X7P FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SAM:S~~-~~ADENOSYLMETHIONINE'>SAM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"><~~div style~~='~~overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[1x7o\|1x7o]]~~</~~div~~></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7p OCA], [https://pdbe.org/1x7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x7p RCSB], [https://www.ebi.ac.uk/pdbsum/1x7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x7p ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7p OCA], [https://pdbe.org/1x7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x7p RCSB], [https://www.ebi.ac.uk/pdbsum/1x7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x7p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/AVRB_STRVR AVRB_STRVR]] Specifically methylates the 2'-O-ribose position of uridine-2479 in 23S ribosomal RNA. Confers resistance to antibiotic avilamycin, an orthosomycin antibiotic.<ref>PMID:11181344</ref> <ref>PMID:12828631</ref>	+	[https://www.uniprot.org/uniprot/AVRB_STRVR AVRB_STRVR] Specifically methylates the 2'-O-ribose position of uridine-2479 in 23S ribosomal RNA. Confers resistance to antibiotic avilamycin, an orthosomycin antibiotic.<ref>PMID:11181344</ref> <ref>PMID:12828631</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x7p ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x7p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The emergence of antibiotic-resistant bacterial strains is a widespread problem in medical practice and drug design, and each case requires the elucidation of the underlying mechanism. AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide (orthosomycin) antibiotic avilamycin. The structure of AviRb with and without bound cofactor S-adenosyl-L-methionine (AdoMet) was determined, showing that it is a homodimer belonging to the SpoU family within the SPOUT class of methyltransferases. The relationships within this class were analyzed in detail and, in addition, a novel fourth SpoU sequence fingerprint is proposed. Each subunit of AviRb consists of two domains. The N-terminal domain, being related to the ribosomal proteins L30 and L7Ae, is likely to bind RNA. The C-terminal domain is related to all SPOUT methyltransferases, and is responsible for AdoMet-binding, catalysis and dimerization. The cofactor binds at the characteristic knot of the polypeptide in an unusually bent conformation. The transferred methyl group points to a broad cleft formed with the L30-type domain of the other subunit. Measurements of mutant activity revealed four important residues responsible for catalysis and allowed the modeling of a complex between AviRb and the RNA target. The model includes a specificity pocket for uracil but does not contain a base for deprotonating the 2'-O atom of U2479 on methylation.
-
-	Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes.,Mosbacher TG, Bechthold A, Schulz GE J Mol Biol. 2005 Jan 21;345(3):535-45. PMID:15581897<ref>PMID:15581897</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1x7p" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Actinomyces viridochromogenes krainsky 1914]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Bechthold, A~~]]	+	[[Category: Streptomyces viridochromogenes]]
-	[[Category: ~~Mosbacher, T G~~]]	+	[[Category: Bechthold A]]
-	[[Category: ~~Schulz, G E~~]]	+	[[Category: Mosbacher TG]]
-	[[Category: ~~Bound cofactor adomet]]~~	+	[[Category: Schulz GE]]
-	~~[[Category: C-terminal knot]]~~	+
-	~~[[Category: Spou]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:05, 21 April 2021

OCA — Wed, 21 Apr 2021 09:05:09 GMT

←Older revision		Revision as of 09:05, 21 April 2021
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	<StructureSection load='1x7p' size='340' side='right'caption='[[1x7p]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1x7p' size='340' side='right'caption='[[1x7p]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1x7p]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/"actinomyces_viridochromogenes"_krainsky_1914 "actinomyces viridochromogenes" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1X7P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1x7p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"actinomyces_viridochromogenes"_krainsky_1914 "actinomyces viridochromogenes" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X7P FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x7o\|1x7o]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1x7o\|1x7o]]</div></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1x7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7p OCA], [~~http~~://pdbe.org/1x7p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1x7p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1x7p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1x7p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7p OCA], [https://pdbe.org/1x7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x7p RCSB], [https://www.ebi.ac.uk/pdbsum/1x7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x7p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/AVRB_STRVR AVRB_STRVR]] Specifically methylates the 2'-O-ribose position of uridine-2479 in 23S ribosomal RNA. Confers resistance to antibiotic avilamycin, an orthosomycin antibiotic.<ref>PMID:11181344</ref> <ref>PMID:12828631</ref>	+	[[https://www.uniprot.org/uniprot/AVRB_STRVR AVRB_STRVR]] Specifically methylates the 2'-O-ribose position of uridine-2479 in 23S ribosomal RNA. Confers resistance to antibiotic avilamycin, an orthosomycin antibiotic.<ref>PMID:11181344</ref> <ref>PMID:12828631</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]

OCA at 13:07, 17 July 2019

OCA — Wed, 17 Jul 2019 13:07:34 GMT

←Older revision		Revision as of 13:07, 17 July 2019
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	==Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes in complex with the cofactor AdoMet==		==Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes in complex with the cofactor AdoMet==
-	<StructureSection load='1x7p' size='340' side='right' caption='[[1x7p]], [[Resolution\|resolution]] 2.55Å' scene=''>	+	<StructureSection load='1x7p' size='340' side='right'caption='[[1x7p]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1x7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_viridochromogenes"_krainsky_1914 "actinomyces viridochromogenes" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X7P FirstGlance]. <br>		<table><tr><td colspan='2'>[[1x7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_viridochromogenes"_krainsky_1914 "actinomyces viridochromogenes" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X7P FirstGlance]. <br>
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	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x7/1x7p_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x7/1x7p_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
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	</StructureSection>		</StructureSection>
	[[Category: Actinomyces viridochromogenes krainsky 1914]]		[[Category: Actinomyces viridochromogenes krainsky 1914]]
		+	[[Category: Large Structures]]
	[[Category: Bechthold, A]]		[[Category: Bechthold, A]]
	[[Category: Mosbacher, T G]]		[[Category: Mosbacher, T G]]

OCA at 15:16, 12 October 2017

OCA — Thu, 12 Oct 2017 15:16:28 GMT

←Older revision		Revision as of 15:16, 12 October 2017
Line 1:		Line 1:
		+
	==Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes in complex with the cofactor AdoMet==		==Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes in complex with the cofactor AdoMet==
	<StructureSection load='1x7p' size='340' side='right' caption='[[1x7p]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='1x7p' size='340' side='right' caption='[[1x7p]], [[Resolution\|resolution]] 2.55Å' scene=''>
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x7o\|1x7o]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x7o\|1x7o]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7p OCA], [http://pdbe.org/1x7p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x7p RCSB], [http://www.ebi.ac.uk/pdbsum/1x7p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7p OCA], [http://pdbe.org/1x7p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x7p RCSB], [http://www.ebi.ac.uk/pdbsum/1x7p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1x7p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x7p ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 02:09, 12 September 2015

OCA — Sat, 12 Sep 2015 02:09:32 GMT

←Older revision		Revision as of 02:09, 12 September 2015
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	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x7o\|1x7o]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x7o\|1x7o]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x7p RCSB], [http://www.ebi.ac.uk/pdbsum/1x7p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7p OCA], [http://pdbe.org/1x7p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x7p RCSB], [http://www.ebi.ac.uk/pdbsum/1x7p PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
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	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1x7p" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 22:29, 25 December 2014

OCA — Thu, 25 Dec 2014 22:29:49 GMT

←Older revision		Revision as of 22:29, 25 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1x7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_viridochromogenes"_krainsky_1914 "actinomyces viridochromogenes" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X7P FirstGlance]. <br>		<table><tr><td colspan='2'>[[1x7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_viridochromogenes"_krainsky_1914 "actinomyces viridochromogenes" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X7P FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x7o\|1x7o]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x7o\|1x7o]]</td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x7p RCSB], [http://www.ebi.ac.uk/pdbsum/1x7p PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x7p RCSB], [http://www.ebi.ac.uk/pdbsum/1x7p PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/AVRB_STRVR AVRB_STRVR]] Specifically methylates the 2'-O-ribose position of uridine-2479 in 23S ribosomal RNA. Confers resistance to antibiotic avilamycin, an orthosomycin antibiotic.<ref>PMID:11181344</ref> <ref>PMID:12828631</ref>
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
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	</StructureSection>		</StructureSection>
	[[Category: Actinomyces viridochromogenes krainsky 1914]]		[[Category: Actinomyces viridochromogenes krainsky 1914]]
-	[[Category: Bechthold, A.]]	+	[[Category: Bechthold, A]]
-	[[Category: Mosbacher, T G.]]	+	[[Category: Mosbacher, T G]]
-	[[Category: Schulz, G E.]]	+	[[Category: Schulz, G E]]
	[[Category: Bound cofactor adomet]]		[[Category: Bound cofactor adomet]]
	[[Category: C-terminal knot]]		[[Category: C-terminal knot]]
	[[Category: Spou]]		[[Category: Spou]]
	[[Category: Transferase]]		[[Category: Transferase]]

OCA at 11:34, 3 October 2014

OCA — Fri, 03 Oct 2014 11:34:53 GMT

←Older revision		Revision as of 11:34, 3 October 2014
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-	~~{{STRUCTURE_1x7p\| PDB=1x7p \| SCENE= }}~~	+	==Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes in complex with the cofactor AdoMet==
-	===Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes in complex with the cofactor AdoMet===	+	<StructureSection load='1x7p' size='340' side='right' caption='[[1x7p]], [[Resolution\|resolution]] 2.55Å' scene=''>
-	~~{{ABSTRACT_PUBMED_15581897}}~~	+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1x7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_viridochromogenes"_krainsky_1914 "actinomyces viridochromogenes" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X7P FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x7o\|1x7o]]</td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x7p OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x7p RCSB], [http://www.ebi.ac.uk/pdbsum/1x7p PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x7/1x7p_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The emergence of antibiotic-resistant bacterial strains is a widespread problem in medical practice and drug design, and each case requires the elucidation of the underlying mechanism. AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide (orthosomycin) antibiotic avilamycin. The structure of AviRb with and without bound cofactor S-adenosyl-L-methionine (AdoMet) was determined, showing that it is a homodimer belonging to the SpoU family within the SPOUT class of methyltransferases. The relationships within this class were analyzed in detail and, in addition, a novel fourth SpoU sequence fingerprint is proposed. Each subunit of AviRb consists of two domains. The N-terminal domain, being related to the ribosomal proteins L30 and L7Ae, is likely to bind RNA. The C-terminal domain is related to all SPOUT methyltransferases, and is responsible for AdoMet-binding, catalysis and dimerization. The cofactor binds at the characteristic knot of the polypeptide in an unusually bent conformation. The transferred methyl group points to a broad cleft formed with the L30-type domain of the other subunit. Measurements of mutant activity revealed four important residues responsible for catalysis and allowed the modeling of a complex between AviRb and the RNA target. The model includes a specificity pocket for uracil but does not contain a base for deprotonating the 2'-O atom of U2479 on methylation.

-	~~==Function==~~	+	Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes.,Mosbacher TG, Bechthold A, Schulz GE J Mol Biol. 2005 Jan 21;345(3):535-45. PMID:15581897<ref>PMID:15581897</ref>
-	~~[[http://www.uniprot.org/uniprot/AVRB_STRVR AVRB_STRVR]] Specifically methylates~~ the 2'-~~O-ribose position of uridine-2479 in 23S ribosomal RNA~~. ~~Confers resistance to antibiotic avilamycin~~, ~~an orthosomycin antibiotic~~.~~<ref>~~PMID:~~11181344</ref>~~ <ref>PMID:~~12828631~~</ref>	+

-	~~==About this Structure==~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	~~[[1x7p]] is~~ a ~~2 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/"actinomyces_viridochromogenes"_krainsky_1914 "actinomyces viridochromogenes" krainsky 1914]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA].~~	+	</div>
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	~~<ref group="xtra">PMID:015581897</ref>~~<references ~~group="xtra"~~/><~~references~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Actinomyces viridochromogenes krainsky 1914]]		[[Category: Actinomyces viridochromogenes krainsky 1914]]
	[[Category: Bechthold, A.]]		[[Category: Bechthold, A.]]

OCA at 07:58, 16 April 2014

OCA — Wed, 16 Apr 2014 07:58:03 GMT

←Older revision		Revision as of 07:58, 16 April 2014
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-	[[Image:1x7p.png\|left\|200px]]
-
	{{STRUCTURE_1x7p\| PDB=1x7p \| SCENE= }}		{{STRUCTURE_1x7p\| PDB=1x7p \| SCENE= }}
-
	===Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes in complex with the cofactor AdoMet===		===Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes in complex with the cofactor AdoMet===
		+	{{ABSTRACT_PUBMED_15581897}}

-	~~{{ABSTRACT_PUBMED_15581897}}~~	+	==Function==
		+	[[http://www.uniprot.org/uniprot/AVRB_STRVR AVRB_STRVR]] Specifically methylates the 2'-O-ribose position of uridine-2479 in 23S ribosomal RNA. Confers resistance to antibiotic avilamycin, an orthosomycin antibiotic.<ref>PMID:11181344</ref> <ref>PMID:12828631</ref>

	==About this Structure==		==About this Structure==
-	[[1x7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Streptomyces_viridochromogenes Streptomyces~~ viridochromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA].	+	[[1x7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"actinomyces_viridochromogenes"_krainsky_1914 "actinomyces viridochromogenes" krainsky 1914]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:015581897</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015581897</ref><references group="xtra"/><references/>
-	[[Category: ~~Streptomyces~~ viridochromogenes]]	+	[[Category: Actinomyces viridochromogenes krainsky 1914]]
	[[Category: Bechthold, A.]]		[[Category: Bechthold, A.]]
	[[Category: Mosbacher, T G.]]		[[Category: Mosbacher, T G.]]

OCA: Protected "1x7p" [edit=sysop:move=sysop]

OCA — Sat, 05 Jan 2013 13:19:02 GMT

Protected "1x7p" [edit=sysop:move=sysop]

←Older revision

Revision as of 13:19, 5 January 2013

OCA at 13:19, 5 January 2013

OCA — Sat, 05 Jan 2013 13:19:00 GMT

←Older revision		Revision as of 13:19, 5 January 2013
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-	{{Seed}}
	[[Image:1x7p.png\|left\|200px]]		[[Image:1x7p.png\|left\|200px]]

-	<!--
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-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
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	{{STRUCTURE_1x7p\| PDB=1x7p \| SCENE= }}		{{STRUCTURE_1x7p\| PDB=1x7p \| SCENE= }}

	===Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes in complex with the cofactor AdoMet===		===Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes in complex with the cofactor AdoMet===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15581897}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15581897 is the PubMed ID number.
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	{{ABSTRACT_PUBMED_15581897}}		{{ABSTRACT_PUBMED_15581897}}

	==About this Structure==		==About this Structure==
-	~~1X7P~~ is a 2 ~~chains~~ structure ~~of sequences~~ from [http://en.wikipedia.org/wiki/Streptomyces_viridochromogenes Streptomyces viridochromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA].	+	[[1x7p]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Streptomyces_viridochromogenes Streptomyces viridochromogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X7P OCA].

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15581897~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015581897</ref><references group="xtra"/>
	[[Category: Streptomyces viridochromogenes]]		[[Category: Streptomyces viridochromogenes]]
	[[Category: Bechthold, A.]]		[[Category: Bechthold, A.]]
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	[[Category: C-terminal knot]]		[[Category: C-terminal knot]]
	[[Category: Spou]]		[[Category: Spou]]
-		+	[[Category: Transferase]]
-	~~''Page seeded by~~ [~~http~~:~~//oca.weizmann.ac.il/oca OCA~~ ] ~~on Wed Feb 18 09:46:05 2009''~~	+