1x8g - Revision history

OCA at 06:18, 3 April 2024

OCA — Wed, 03 Apr 2024 06:18:28 GMT

←Older revision		Revision as of 06:18, 3 April 2024
Line 3:		Line 3:
	<StructureSection load='1x8g' size='340' side='right'caption='[[1x8g]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1x8g' size='340' side='right'caption='[[1x8g]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1x8g]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"aeromonas_liquefaciens"_kluyver_and_van_niel_1936 "aeromonas liquefaciens" kluyver and van niel 1936~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=1X8G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1x8g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1X8G FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>~~, ~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.7Å</td></tr>
-	~~<tr id='related'><td class="sblockLbl"><b>~~[[~~Related_structure~~\|~~Related:~~]]~~</b></td><td class="sblockDat"><div style='overflow: auto~~; ~~max-height: 3em;'>[[1x8h\|1x8h]], [[1x8i\|1x8i]]</div>~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>cphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~644~~ "~~Aeromonas liquefaciens~~" ~~Kluyver and van Niel 1936])~~<~~/td~~></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1x8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8g OCA], [https://pdbe.org/1x8g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1x8g RCSB], [https://www.ebi.ac.uk/pdbsum/1x8g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1x8g ProSAT]</span></td></tr>
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6]~~ </~~span~~></td></tr>	+
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=1x8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8g OCA], [~~http~~://pdbe.org/1x8g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=1x8g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/1x8g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=1x8g ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/BLAB_AERHY BLAB_AERHY]] Can hydrolyze carbapenem compounds.	+	[https://www.uniprot.org/uniprot/BLAB_AERHY BLAB_AERHY] Can hydrolyze carbapenem compounds.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 22:		Line 20:
	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x8g ConSurf].		</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x8g ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	One strategy developed by bacteria to resist the action of beta-lactam antibiotics is the expression of metallo-beta-lactamases. CphA from Aeromonas hydrophila is a member of a clinically important subclass of metallo-beta-lactamases that have only one zinc ion in their active site and for which no structure is available. The crystal structures of wild-type CphA and its N220G mutant show the structural features of the active site of this enzyme, which is modeled specifically for carbapenem hydrolysis. The structure of CphA after reaction with a carbapenem substrate, biapenem, reveals that the enzyme traps a reaction intermediate in the active site. These three X-ray structures have allowed us to propose how the enzyme recognizes carbapenems and suggest a mechanistic pathway for hydrolysis of the beta-lactam. This will be relevant for the design of metallo-beta-lactamase inhibitors as well as of antibiotics that escape their hydrolytic activity.
-
-	A metallo-beta-lactamase enzyme in action: crystal structures of the monozinc carbapenemase CphA and its complex with biapenem.,Garau G, Bebrone C, Anne C, Galleni M, Frere JM, Dideberg O J Mol Biol. 2005 Jan 28;345(4):785-95. PMID:15588826<ref>PMID:15588826</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 1x8g" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]		*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aeromonas ~~liquefaciens kluyver and van niel 1936]]~~	+	[[Category: Aeromonas hydrophila]]
-	~~[[Category: Beta-lactamase~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dideberg, O]]	+	[[Category: Dideberg O]]
-	[[Category: Garau, G]]	+	[[Category: Garau G]]
-	~~[[Category: Hydrolase~~]]	+

OCA at 09:47, 6 January 2021

OCA — Wed, 06 Jan 2021 09:47:38 GMT

←Older revision		Revision as of 09:47, 6 January 2021
Line 1:		Line 1:

	==Crystal Structure of the Mono-Zinc Carbapenemase CphA from Aeromonas Hydrophyla==		==Crystal Structure of the Mono-Zinc Carbapenemase CphA from Aeromonas Hydrophyla==
-	<StructureSection load='1x8g' size='340' side='right' caption='[[1x8g]], [[Resolution\|resolution]] 1.70Å' scene=''>	+	<StructureSection load='1x8g' size='340' side='right'caption='[[1x8g]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1x8g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aeromonas_liquefaciens"_kluyver_and_van_niel_1936 "aeromonas liquefaciens" kluyver and van niel 1936]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1X8G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1x8g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aeromonas_liquefaciens"_kluyver_and_van_niel_1936 "aeromonas liquefaciens" kluyver and van niel 1936]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1X8G FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x8h\|1x8h]], [[1x8i\|1x8i]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1x8h\|1x8h]], [[1x8i\|1x8i]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=644 "Aeromonas liquefaciens" Kluyver and van Niel 1936])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=644 "Aeromonas liquefaciens" Kluyver and van Niel 1936])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=1x8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8g OCA], [http://pdbe.org/1x8g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x8g RCSB], [http://www.ebi.ac.uk/pdbsum/1x8g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1x8g ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1x8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8g OCA], [http://pdbe.org/1x8g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x8g RCSB], [http://www.ebi.ac.uk/pdbsum/1x8g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1x8g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 33:		Line 33:

	==See Also==		==See Also==
-	*[[Beta-lactamase\|Beta-lactamase]]	+	*[[Beta-lactamase 3D structures\|Beta-lactamase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
Line 40:		Line 40:
	[[Category: Aeromonas liquefaciens kluyver and van niel 1936]]		[[Category: Aeromonas liquefaciens kluyver and van niel 1936]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
		+	[[Category: Large Structures]]
	[[Category: Dideberg, O]]		[[Category: Dideberg, O]]
	[[Category: Garau, G]]		[[Category: Garau, G]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 07:38, 18 April 2018

OCA — Wed, 18 Apr 2018 07:38:34 GMT

←Older revision		Revision as of 07:38, 18 April 2018
Line 1:		Line 1:
		+
	==Crystal Structure of the Mono-Zinc Carbapenemase CphA from Aeromonas Hydrophyla==		==Crystal Structure of the Mono-Zinc Carbapenemase CphA from Aeromonas Hydrophyla==
	<StructureSection load='1x8g' size='340' side='right' caption='[[1x8g]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1x8g' size='340' side='right' caption='[[1x8g]], [[Resolution\|resolution]] 1.70Å' scene=''>
Line 7:		Line 8:
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=644 "Aeromonas liquefaciens" Kluyver and van Niel 1936])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=644 "Aeromonas liquefaciens" Kluyver and van Niel 1936])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8g OCA], [http://pdbe.org/1x8g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x8g RCSB], [http://www.ebi.ac.uk/pdbsum/1x8g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8g OCA], [http://pdbe.org/1x8g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x8g RCSB], [http://www.ebi.ac.uk/pdbsum/1x8g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1x8g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 15:		Line 16:
	Check<jmol>		Check<jmol>
	<jmolCheckbox>		<jmolCheckbox>
-	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/1x8g_consurf.spt"</scriptWhenChecked>	+	<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/1x8g_consurf.spt"</scriptWhenChecked>
	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>		<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>

OCA at 06:54, 8 February 2016

OCA — Mon, 08 Feb 2016 06:54:13 GMT

←Older revision		Revision as of 06:54, 8 February 2016
Line 19:		Line 19:
	<text>to colour the structure by Evolutionary Conservation</text>		<text>to colour the structure by Evolutionary Conservation</text>
	</jmolCheckbox>		</jmolCheckbox>
-	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/~~chain_selection~~.php?pdb_ID=~~2ata~~ ConSurf].	+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1x8g ConSurf].
	<div style="clear:both"></div>		<div style="clear:both"></div>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">

OCA at 09:00, 10 September 2015

OCA — Thu, 10 Sep 2015 09:00:46 GMT

←Older revision		Revision as of 09:00, 10 September 2015
Line 2:		Line 2:
	<StructureSection load='1x8g' size='340' side='right' caption='[[1x8g]], [[Resolution\|resolution]] 1.70Å' scene=''>		<StructureSection load='1x8g' size='340' side='right' caption='[[1x8g]], [[Resolution\|resolution]] 1.70Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[1x8g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/~~Aeromonas_hydrophila Aeromonas hydrophila~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X8G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[1x8g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"aeromonas_liquefaciens"_kluyver_and_van_niel_1936 "aeromonas liquefaciens" kluyver and van niel 1936]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X8G FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x8h\|1x8h]], [[1x8i\|1x8i]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x8h\|1x8h]], [[1x8i\|1x8i]]</td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=644 Aeromonas ~~hydrophila~~])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=644 "Aeromonas liquefaciens" Kluyver and van Niel 1936])</td></tr>
	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>		<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x8g RCSB], [http://www.ebi.ac.uk/pdbsum/1x8g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8g OCA], [http://pdbe.org/1x8g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1x8g RCSB], [http://www.ebi.ac.uk/pdbsum/1x8g PDBsum]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 29:		Line 29:
	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>		From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
	</div>		</div>
		+	<div class="pdbe-citations 1x8g" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
Line 36:		Line 37:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Aeromonas ~~hydrophila~~]]	+	[[Category: Aeromonas liquefaciens kluyver and van niel 1936]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
	[[Category: Dideberg, O]]		[[Category: Dideberg, O]]
	[[Category: Garau, G]]		[[Category: Garau, G]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 21:27, 24 December 2014

OCA — Wed, 24 Dec 2014 21:27:53 GMT

←Older revision		Revision as of 21:27, 24 December 2014
Line 3:		Line 3:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[1x8g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X8G FirstGlance]. <br>		<table><tr><td colspan='2'>[[1x8g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X8G FirstGlance]. <br>
-	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x8h\|1x8h]], [[1x8i\|1x8i]]</td></tr>	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x8h\|1x8h]], [[1x8i\|1x8i]]</td></tr>
-	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=644 Aeromonas hydrophila])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=644 Aeromonas hydrophila])</td></tr>
-	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>	+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
-	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x8g RCSB], [http://www.ebi.ac.uk/pdbsum/1x8g PDBsum]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x8g RCSB], [http://www.ebi.ac.uk/pdbsum/1x8g PDBsum]</span></td></tr>
-	<table>	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BLAB_AERHY BLAB_AERHY]] Can hydrolyze carbapenem compounds.
	== Evolutionary Conservation ==		== Evolutionary Conservation ==
	[[Image:Consurf_key_small.gif\|200px\|right]]		[[Image:Consurf_key_small.gif\|200px\|right]]
Line 36:		Line 38:
	[[Category: Aeromonas hydrophila]]		[[Category: Aeromonas hydrophila]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]
-	[[Category: Dideberg, O.]]	+	[[Category: Dideberg, O]]
-	[[Category: Garau, G.]]	+	[[Category: Garau, G]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 23:38, 28 September 2014

OCA — Sun, 28 Sep 2014 23:38:58 GMT

←Older revision		Revision as of 23:38, 28 September 2014
Line 1:		Line 1:
-	[[~~Image:~~1x8g.~~png~~\|~~left~~\|200px]]	+	==Crystal Structure of the Mono-Zinc Carbapenemase CphA from Aeromonas Hydrophyla==
		+	<StructureSection load='1x8g' size='340' side='right' caption='[[1x8g]], [[Resolution\|resolution]] 1.70Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[1x8g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1X8G FirstGlance]. <br>
		+	</td></tr><tr><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO3:CARBONATE+ION'>CO3</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene><br>
		+	<tr><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[1x8h\|1x8h]], [[1x8i\|1x8i]]</td></tr>
		+	<tr><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">cphA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=644 Aeromonas hydrophila])</td></tr>
		+	<tr><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr>
		+	<tr><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1x8g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1x8g OCA], [http://www.rcsb.org/pdb/explore.do?structureId=1x8g RCSB], [http://www.ebi.ac.uk/pdbsum/1x8g PDBsum]</span></td></tr>
		+	<table>
		+	== Evolutionary Conservation ==
		+	[[Image:Consurf_key_small.gif\|200px\|right]]
		+	Check<jmol>
		+	<jmolCheckbox>
		+	<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/x8/1x8g_consurf.spt"</scriptWhenChecked>
		+	<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
		+	<text>to colour the structure by Evolutionary Conservation</text>
		+	</jmolCheckbox>
		+	</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary\|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
		+	<div style="clear:both"></div>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	One strategy developed by bacteria to resist the action of beta-lactam antibiotics is the expression of metallo-beta-lactamases. CphA from Aeromonas hydrophila is a member of a clinically important subclass of metallo-beta-lactamases that have only one zinc ion in their active site and for which no structure is available. The crystal structures of wild-type CphA and its N220G mutant show the structural features of the active site of this enzyme, which is modeled specifically for carbapenem hydrolysis. The structure of CphA after reaction with a carbapenem substrate, biapenem, reveals that the enzyme traps a reaction intermediate in the active site. These three X-ray structures have allowed us to propose how the enzyme recognizes carbapenems and suggest a mechanistic pathway for hydrolysis of the beta-lactam. This will be relevant for the design of metallo-beta-lactamase inhibitors as well as of antibiotics that escape their hydrolytic activity.

-	~~{{STRUCTURE_1x8g\| PDB=1x8g \| SCENE= }}~~	+	A metallo-beta-lactamase enzyme in action: crystal structures of the monozinc carbapenemase CphA and its complex with biapenem.,Garau G, Bebrone C, Anne C, Galleni M, Frere JM, Dideberg O J Mol Biol. 2005 Jan 28;345(4):785-95. PMID:15588826<ref>PMID:15588826</ref>

-	~~===Crystal Structure~~ of the ~~Mono-Zinc Carbapenemase CphA from Aeromonas Hydrophyla===~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-		+	</div>
-	~~{{ABSTRACT_PUBMED_15588826}}~~	+
-		+
-	~~==About this Structure==~~	+
-	~~[[1x8g]] is a 1 chain structure with sequence from [http://en~~.~~wikipedia~~.~~org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]~~. ~~Full crystallographic information is available from [http:~~/~~/oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA].~~	+

	==See Also==		==See Also==
	*[[Beta-lactamase\|Beta-lactamase]]		*[[Beta-lactamase\|Beta-lactamase]]
-		+	== References ==
-	==~~Reference~~==	+	<references/>
-	<~~ref group="xtra">PMID:015588826<~~/~~ref~~><~~ref group="xtra">PMID:015215079</ref><ref group="xtra">PMID:007588620</ref><references group="xtra"~~/>	+	__TOC__
		+	</StructureSection>
	[[Category: Aeromonas hydrophila]]		[[Category: Aeromonas hydrophila]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]

OCA at 09:18, 20 October 2012

OCA — Sat, 20 Oct 2012 09:18:38 GMT

←Older revision		Revision as of 09:18, 20 October 2012
Line 8:		Line 8:

	==About this Structure==		==About this Structure==
-	[[1x8g]] is a 1 chain structure ~~of [[Beta-lactamase]]~~ with sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA].	+	[[1x8g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA].

	==See Also==		==See Also==
Line 14:		Line 14:

	==Reference==		==Reference==
-	<ref group="xtra">PMID:015588826</ref><ref group="xtra">PMID:015215079</ref><ref group="xtra">PMID:007588620~~</ref><ref group="xtra">PMID:012507470~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015588826</ref><ref group="xtra">PMID:015215079</ref><ref group="xtra">PMID:007588620</ref><references group="xtra"/>
	[[Category: Aeromonas hydrophila]]		[[Category: Aeromonas hydrophila]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]

OCA at 22:24, 25 July 2012

OCA — Wed, 25 Jul 2012 22:24:36 GMT

←Older revision		Revision as of 22:24, 25 July 2012
Line 1:		Line 1:
	[[Image:1x8g.png\|left\|200px]]		[[Image:1x8g.png\|left\|200px]]

-	<!--
-	The line below this paragraph, containing "STRUCTURE_1x8g", creates the "Structure Box" on the page.
-	You may change the PDB parameter (which sets the PDB file loaded into the applet)
-	or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-	or leave the SCENE parameter empty for the default display.
-	-->
	{{STRUCTURE_1x8g\| PDB=1x8g \| SCENE= }}		{{STRUCTURE_1x8g\| PDB=1x8g \| SCENE= }}

	===Crystal Structure of the Mono-Zinc Carbapenemase CphA from Aeromonas Hydrophyla===		===Crystal Structure of the Mono-Zinc Carbapenemase CphA from Aeromonas Hydrophyla===

-
-	<!--
-	The line below this paragraph, {{ABSTRACT_PUBMED_15588826}}, adds the Publication Abstract to the page
-	(as it appears on PubMed at http://www.pubmed.gov), where 15588826 is the PubMed ID number.
-	-->
	{{ABSTRACT_PUBMED_15588826}}		{{ABSTRACT_PUBMED_15588826}}

	==About this Structure==		==About this Structure==
-	[[1x8g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA].	+	[[1x8g]] is a 1 chain structure of [[Beta-lactamase]] with sequence from [http://en.wikipedia.org/wiki/Aeromonas_hydrophila Aeromonas hydrophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X8G OCA].
		+
		+	==See Also==
		+	*[[Beta-lactamase\|Beta-lactamase]]

	==Reference==		==Reference==
-	<ref group="xtra">PMID:~~15588826~~</ref><ref group="xtra">PMID:~~15215079~~</ref><ref group="xtra">PMID:~~7588620~~</ref><ref group="xtra">PMID:~~12507470~~</ref><references group="xtra"/>	+	<ref group="xtra">PMID:015588826</ref><ref group="xtra">PMID:015215079</ref><ref group="xtra">PMID:007588620</ref><ref group="xtra">PMID:012507470</ref><references group="xtra"/>
	[[Category: Aeromonas hydrophila]]		[[Category: Aeromonas hydrophila]]
	[[Category: Beta-lactamase]]		[[Category: Beta-lactamase]]

OCA: Protected "1x8g" [edit=sysop:move=sysop]

OCA — Mon, 14 Mar 2011 22:57:38 GMT

Protected "1x8g" [edit=sysop:move=sysop]

←Older revision

Revision as of 22:57, 14 March 2011